ViewGene
Locus Name:
GeneID:
Location:

GeneID:3244
TrivialName: PA14_57050
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57050
PAO1 Ortholog Locus:PA4389
Sequence Length:759
Protein Length:252
Start: 5082597
Stop: 5083355
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07777.1|AE004854_13 (AE004854) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 252/252 (100%), Positives = 252/252 (100%)
Sequence:
ATGCAATTGAAAGACAAGGTCATCATCATCACCGGCGGCTGCCAGGGGCTCGGTCGGGCAATGGGCGAATAT
CTCGCCGGCAAGGGCGCCAGGCTGGCCCTGGTCGATCTCAACCGCGAACGTCTCGACGAAGCCGTCGCTGCC
TGCAAGGCCGCCGGCGGCGACGCTCGCGCCTACGTGTGCAACGTGGCGGACGAGGAACAGGTTACCCACATG
GTGGCCCAGGTCGCCAGCGACTTCGGCGCGATCAACGGCCTGGTCAACAACGCCGGCATCCTCCGCGACGGC
CTGACCATCAAGGTCAAGGACGGCCAGTTGAGCAAGATGAGCCTGGCCCAATGGCAATCGGTGATCGACGTC
AACCTCACCGGTGTGTTCCTTTGCACCCGCGAAGTGGCGGCGAAGATGATCGAGCTGAAGAACGAAGGCGCG
ATCGTCAACATTTCCTCGATCTCCCGCGCCGGCAACATGGGCCAGGCCAACTACTCGGCAGCCAAGGCCGGG
GTCGCCGCGGATACCGTGGTCTGGGCGAAGGAACTGGCGCGCTACGGCATCCGCGTGGCCGGCGTGGCGCCG
GGCTTCATCGAGACCGAGATGACCGCCGGGATGAAACCCGAAGCGCTGGAGAAGATGACCGCCGGCATCCCT
CTGAAGCGCATGGGCAAGCCGGTGGAGATCGCCCATTCGGTGGCCTACATCTTCGAGAACGACTACTACACC
GGCCGCGTTCTCGAACTGGACGGCGGCCTGCGCCTGTAG
Translation:
MQLKDKVIIITGGCQGLGRAMGEYLAGKGARLALVDLNRERLDEAVAACKAAGGDARAYVCNVADEEQVTHM
VAQVASDFGAINGLVNNAGILRDGLTIKVKDGQLSKMSLAQWQSVIDVNLTGVFLCTREVAAKMIELKNEGA
IVNISSISRAGNMGQANYSAAKAGVAADTVVWAKELARYGIRVAGVAPGFIETEMTAGMKPEALEKMTAGIP
LKRMGKPVEIAHSVAYIFENDYYTGRVLELDGGLRL*
AnnotationID:3252GeneID:3244
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 12:12:31
Gene Name:
Confidence Code:3
GeneProduct:putative short chain dehydrogenase
Cell Localization:()
Synonyms:PA4389
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:KOG1200,pfam00106,COG1028,KOG1199
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57830.1| (AE016871) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 252

 Score =  401 bits (1031), Expect = e-111
 Identities = 198/252 (78%), Positives = 229/252 (90%)

>gb|AAN54738.1|AE015614_5 (AE015614) 3-oxoacyl-(acyl-carrier-protein) reductase, putative
           [Shewanella oneidensis MR-1]
          Length = 252

 Score =  275 bits (703), Expect = 2e-73
 Identities = 144/253 (56%), Positives = 185/253 (73%), Gaps = 2/253 (0%)

>dbj|BAC62460.1| (AP005087) putative short-chain dehydrogenase [Vibrio
           parahaemolyticus]
          Length = 252

 Score =  273 bits (699), Expect = 5e-73
 Identities = 136/252 (53%), Positives = 183/252 (72%)







 
Structural Features:
KOG1200, Mitochondrial/plastidial beta-ketoacyl-ACP reductase 
[Lipid transport and metabolism]
CD-Length = 256 residues,  95.3% aligned
Score =  196 bits (499), Expect = 4e-51
Identities = 108/253 (42%), Positives = 148/253 (58%), Gaps = 13/253 (5%)

pfam00106, adh_short, short chain dehydrogenase. 
CD-Length = 247 residues, 100.0% aligned
Score =  179 bits (456), Expect = 4e-46
Identities = 96/256 (37%), Positives = 140/256 (54%), Gaps = 22/256 (8%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues, 100.0% aligned
Score =  162 bits (411), Expect = 7e-41
Identities = 110/262 (41%), Positives = 147/262 (56%), Gaps = 22/262 (8%)

KOG1199, Short-chain alcohol dehydrogenase/3-hydroxyacyl-CoA dehydrogenase 
[Secondary metabolites biosynthesis, transport and catabolism]
CD-Length = 260 residues,  96.9% aligned
Score =  133 bits (335), Expect = 5e-32
Identities = 92/257 (35%), Positives = 131/257 (50%), Gaps = 12/257 (4%)







   
Genomic Context:






   
Comment:


















    ReferenceID:6491




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19824




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3244_cdd.html


    		




 
URL:

















    ReferenceID:23633




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3244
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5219
                           PA4389_tr
                           translation of PA4389
                           252
                           99.99
                           99.99
                           0
                           1265.0
     

     
     
146163
                           gb|AAG07777.1|AE004854_13
                           (AE004854) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           252
                           99.99
                           99.99
                           0
                           1265.0
     

     
     
146164
                           gb|AAO57830.1|
                           (AE016871) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas syringae pv. tomato str. DC3000]
                           252
                           78.57
                           90.87
                           0
                           1031.0
     

     
     
146165
                           gb|AAO07439.1|AE016809_201
                           (AE016809) Dehydrogenase [Vibrio vulnificus CMCP6]
                           252
                           58.33
                           69.84
                           0
                           726.0
     

     
     
146166
                           dbj|BAC97063.1|
                           (AP005348) dehydrogenase [Vibrio vulnificus YJ016]
                           252
                           57.93
                           69.44
                           0
                           720.0
     

     






      

          
GC ORFID: 46246How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4389Glimmer Score: 

          
Start: 4346273Stop: 4347031

          
Length: 759


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4389 translation of PA4389Homolog Bit Score 491.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene1988 (ORF: BLASTX 406.0), t_PA4389 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAATTGAAAGACAAGGTCATCATCATCACCGGCGGCTGCCAGGGGCTCGGTCGGGCAATGGGCGAATAT 
CTCGCCGGCAAGGGCGCCAGGCTGGCCCTGGTCGATCTCAACCGCGAACGTCTCGACGAAGCCGTCGCTGCC 
TGCAAGGCCGCCGGCGGCGACGCTCGCGCCTACGTGTGCAACGTGGCGGACGAGGAACAGGTTACCCACATG 
GTGGCCCAGGTCGCCAGCGACTTCGGCGCGATCAACGGCCTGGTCAACAACGCCGGCATCCTCCGCGACGGC 
CTGACCATCAAGGTCAAGGACGGCCAGTTGAGCAAGATGAGCCTGGCCCAATGGCAATCGGTGATCGACGTC 
AACCTCACCGGTGTGTTCCTTTGCACCCGCGAAGTGGCGGCGAAGATGATCGAGCTGAAGAACGAAGGCGCG 
ATCGTCAACATTTCCTCGATCTCCCGCGCCGGCAACATGGGCCAGGCCAACTACTCGGCAGCCAAGGCCGGG 
GTCGCCGCGGATACCGTGGTCTGGGCGAAGGAACTGGCGCGCTACGGCATCCGCGTGGCCGGCGTGGCGCCG 
GGCTTCATCGAGACCGAGATGACCGCCGGGATGAAACCCGAAGCGCTGGAGAAGATGACCGCCGGCATCCCT 
CTGAAGCGCATGGGCAAGCCGGTGGAGATCGCCCATTCGGTGGCCTACATCTTCGAGAACGACTACTACACC 
GGCCGCGTTCTCGAACTGGACGGCGGCCTGCGCCTGTAG