ViewGene
Locus Name:
GeneID:
Location:

GeneID:3243
TrivialName: PA14_11070
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11070
PAO1 Ortholog Locus:PA4085
Sequence Length:747
Protein Length:248
Start: 957412
Stop: 958158
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07472.1|AE004825_1 (AE004825) chaperone CupB2 [Pseudomonas aeruginosa PAO1]
Identities = 245/248 (98%), Positives = 246/248 (99%)
Sequence:
ATGGCGCCGCTAATGCATCGTTTTCATTCTTTCGTGGCGGCAAGCGCCGTGGCCATCGCCCTGTGCGCCGGA
ACGGCTCATGCCGGGCTGATCGCACAGGGCACTCGCGTCGTCTTTCCGGCCAGCGAGCGCGAGGTCACCTTG
CGGGTCAGCAATACGTCCGGCACTCCCGTGCTGGCGCAAGCCTGGATCGACGATGGCCGGCAGGACGTTCCC
CCCGAGGAACTGCAGGTCCCCTTCAGCGTCACGCCGGCCGTGACGCGAGTGGACCCCAATGGCGGCGCCGTA
TTGCGCATCGCCTACCTGAAGGCCCCGTTGCCGACGGATCGCGAATCGCTGTTCTGGCTGAATATCCTGGAA
GTTCCGCCCAGGGACGAAGACGAAAACAATGCATTGCAGTTTTCCTTTCGTTCGCGCTTCAAACTCTTCTTT
CGACCCAGCCAGTTGAAGAGTGTCGATTCGGCTGCCGGGAAACTTCAATGGAAGTTTCTGGAGTCAGGGGGA
GCAGGAAAAAAGACCGTAGTACAGGTAAATAATCCGACGCCTTACTACGTTTCGTTCGCCAGCGTCGAATTG
ATTGTCGATGGCCGTGTCATGTCCGTGGGAAAGGGAATGGTCGCGCCATTCTCGACGAAGGAGTTCGACTGG
CAGGGAAATCCGAAGAACATGGAAGCCGCTTCCGTCAGATACGAAGTCATCAACGATTACGGTGGCCGCAAT
ACCCTCGATAGGGCACTCGGCAAATAA
Translation:
MAPLMHRFHSFVAASAVAIALCAGTAHAGLIAQGTRVVFPASEREVTLRVSNTSGTPVLAQAWIDDGRQDVP
PEELQVPFSVTPAVTRVDPNGGAVLRIAYLKAPLPTDRESLFWLNILEVPPRDEDENNALQFSFRSRFKLFF
RPSQLKSVDSAAGKLQWKFLESGGAGKKTVVQVNNPTPYYVSFASVELIVDGRVMSVGKGMVAPFSTKEFDW
QGNPKNMEAASVRYEVINDYGGRNTLDRALGK*
AnnotationID:3251GeneID:3243
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 08:08:20
Gene Name:cupB2
Confidence Code:2
GeneProduct:chaperone CupB2
Cell Localization:()
Synonyms:fimC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:P pilus assembly protein
Functional Category Confidence Code:5
COGs:COG3121,pfam00345
Secondary Functional Category(ies):chaperones and heat shock proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG54444.1|AE005190_6 (AE005190) putative fimbrial chaperone protein [Escherichia coli
           O157:H7 EDL933]
          Length = 243

 Score =  179 bits (454), Expect = 1e-44
 Identities = 92/232 (39%), Positives = 138/232 (59%), Gaps = 12/232 (5%)








 
Structural Features:
COG3121, FimC, P pilus assembly protein, chaperone PapD 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 235 residues,  98.7% aligned
Score =  213 bits (544), Expect = 2e-56
Identities = 97/247 (39%), Positives = 133/247 (53%), Gaps = 18/247 (7%)

pfam00345, Pili_assembly_N, Gram-negative pili assembly chaperone, N-terminal domain.
CD-Length = 122 residues, 100.0% aligned
Score =  138 bits (349), Expect = 6e-34
Identities = 53/126 (42%), Positives = 74/126 (58%), Gaps = 8/126 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6489




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23631




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36832




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3243_cdd.html


    		




 
URL:

















    ReferenceID:36941




  
Author/Investigator(s):
        Vallet I, Olson JW, Lory S, Lazdunski A, Filloux A.





  
Title:
        The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation.




  
PubMed:
        11381121


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6911-6. Epub 2001 May 29.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11381121

















    ReferenceID:36944




  
Author/Investigator(s):
        Klemm P, Jorgensen BJ, van Die I, de Ree H, Bergmans H.





  
Title:
        The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization.




  
PubMed:
        2863734


MedLine:
        




    
Source:
Mol Gen Genet. 1985;199(3):410-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2863734









           
Homologs By Global Alignment 

           

       



     
Gene ID:3243
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5218
                           PA4085_tr
                           translation of PA4085
                           248
                           98.79
                           99.19
                           0
                           1264.0
     

     
     
146151
                           gb|AAG07472.1|AE004825_1
                           (AE004825) chaperone CupB2 [Pseudomonas aeruginosa PAO1]
                           248
                           98.79
                           99.19
                           0
                           1264.0
     

     
     
146154
                           emb|CAE42164.1|
                           (BX640416) chaperone protein [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           255
                           40.78
                           55.68
                           18
                           440.0
     

     
     
146155
                           emb|CAA47265.1|
                           (X66729) filamentous hemagglutinin [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           255
                           40.78
                           55.68
                           18
                           440.0
     

     
     
146158
                           emb|CAA46089.1|
                           (X64876) FimB chaperone-like protein precursor [Bordetella pertussis]
                           258
                           39.53
                           55.81
                           24
                           437.0
     

     






      

          
GC ORFID: 46245How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4085Glimmer Score: 

          
Start: 3996525Stop: 3995779

          
Length: 747


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4085 translation of PA4085Homolog Bit Score 491.0

          
Other Homologs:  t_PA4085 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGCCGCTAATGCATCGTTTTCATTCTTTCGTGGCGGCAAGCGCCGTGGCCATCGCCCTGTGCGCCGGA 
ACGGCTCATGCCGGGCTGATCGCACAGGGCACTCGCGTCGTCTTTCCGGCCAGCGAGCGCGAGGTCACCTTG 
CGGGTCAGCAATACGTCCGGCACTCCCGTGCTGGCGCAAGCCTGGATCGACGATGGCCGGCAGGACGTTCCC 
CCCGAGGAACTGCAGGTCCCCTTCAGCGTCACGCCGGCCGTGACGCGAGTGGACCCCAATGGCGGCGCCGTA 
TTGCGCATCGCCTACCTGAAGGCCCCGTTGCCGACGGATCGCGAATCGCTGTTCTGGCTGAATATCCTGGAA 
GTTCCGCCCAGGGACGAAGACGAAAACAATGCATTGCAGTTTTCCTTTCGTTCGCGCTTCAAACTCTTCTTT 
CGACCCAGCCAGTTGAAGAGTGTCGATTCGGCTGCCGGGAAACTTCAATGGAAGTTTCTGGAGTCAGGGGGA 
GCAGGAAAAAAGACCGTAGTACAGGTAAATAATCCGACGCCTTACTACGTTTCGTTCGCCAGCGTCGAATTG 
ATTGTCGATGGCCGTGTCATGTCCGTGGGAAAGGGAATGGTCGCGCCATTCTCGACGAAGGAGTTCGACTGG 
CAGGGAAATCCGAAGAACATGGAAGCCGCTTCCGTCAGATACGAAGTCATCAACGATTACGGTGGCCGCAAT 
ACCCTCGATAGGGCACTCGGCAAATAA