ViewGene
Locus Name:
GeneID:
Location:

GeneID:3242
TrivialName: PA14_14850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14850
PAO1 Ortholog Locus:PA3805
Sequence Length:759
Protein Length:252
Start: 1261203
Stop: 1261961
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07192.1|AE004798_15 (AE004798) type 4 fimbrial biogenesis protein PilF [Pseudomonas aeruginosa PAO1]
Identities = 251/252 (99%), Positives = 251/252 (99%)
Sequence:
ATGACTGTACGCGCCGCGCTGGTGTTCCTGTTGGCAGTTGGGCTGACAGGCTGCGTGACGAGCGGAGATCAG
AATCCCCTGAAAACCGACAAGGGCCGTGACGAGGCGCGCGATGCCTATATCCAGCTCGGCCTCGGCTATCTC
CAGCGGGGGAACACCGAGCAGGCCAAGGTGCCGCTGCGCAAGGCCCTCGAGATCGACCCTTCCAGCGCCGAC
GCGCATGCCGCGCTGGCGGTGGTGTTCCAGACCGAGATGGAGCCCAAGCTGGCCGACGAGGAGTACCGCAAG
GCGCTCGCCAGCGATAGCCGCAATGCCCGGGTACTGAACAACTACGGCGGTTTCCTCTACGAGCAGAAGCGC
TACGAGGAGGCCTACCAGCGTCTCCTGGAGGCCAGCCAGGACACCCTCTATCCCGAGCGCTCGCGGGTCTTC
GAGAACCTCGGCCTGGTTTCGCTGCAAATGAAGAAACCGGCCCAGGCCAAGGAGTACTTCGAGAAATCCCTG
CGTCTGAACCGTAACCAGCCCAGCGTCGCGCTGGAGATGGCCGACCTGCTCTACAAGGAGCGCGAGTACGTG
CCGGCTCGCCAGTACTATGATCTCTTTGCCCAGGGCGGCGGGCAGAACGCCCGCAGCCTGTTGCTGGGCATT
CGTCTCGCGAAGGTCTTCGAGGACCGCGACACGGCCGCCAGCTATGGCCTGCAGTTGAAACGCCTGTACCCA
GGCTCTCCCGAATATCAGGAATTCCAGGCGGAAAAATGA
Translation:
MTVRAALVFLLAVGLTGCVTSGDQNPLKTDKGRDEARDAYIQLGLGYLQRGNTEQAKVPLRKALEIDPSSAD
AHAALAVVFQTEMEPKLADEEYRKALASDSRNARVLNNYGGFLYEQKRYEEAYQRLLEASQDTLYPERSRVF
ENLGLVSLQMKKPAQAKEYFEKSLRLNRNQPSVALEMADLLYKEREYVPARQYYDLFAQGGGQNARSLLLGI
RLAKVFEDRDTAASYGLQLKRLYPGSPEYQEFQAEK*
AnnotationID:3250GeneID:3242
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 11:11:12
Gene Name:pilF
Confidence Code:1
GeneProduct:type 4 fimbrial biogenesis protein PilF
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3063
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54953.1| (AE016860) type IV pilus biogenesis protein PilF [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 252

 Score =  327 bits (839), Expect = 3e-89
 Identities = 159/252 (63%), Positives = 207/252 (82%)

>gb|AAN66476.1|AE016276_11 (AE016777) type IV pili biogenesis protein PilF [Pseudomonas putida
           KT2440]
          Length = 255

 Score =  274 bits (701), Expect = 3e-73
 Identities = 142/252 (56%), Positives = 180/252 (71%)






 
Structural Features:
COG3063, PilF, Tfp pilus assembly protein PilF 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 250 residues, 100.0% aligned
Score =  273 bits (699), Expect = 3e-74
Identities = 113/251 (45%), Positives = 139/251 (55%), Gaps = 1/251 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6487




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23629




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31751




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3242_cdd.html


    		




 
URL:

















    ReferenceID:34281




  
Author/Investigator(s):
        Watson AA, Alm RA, Mattick JS.





  
Title:
        Identification of a gene, pilF, required for type 4 fimbrial biogenesis and twitching motility in Pseudomonas aeruginosa.




  
PubMed:
        8973346


MedLine:
        




    
Source:
Gene. 1996 Nov 21;180(1-2):49-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8973346









           
Homologs By Global Alignment 

           

       



     
Gene ID:3242
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5217
                           PA3805_tr
                           translation of PA3805
                           252
                           99.60
                           99.60
                           0
                           1263.0
     

     
     
146147
                           gb|AAG07192.1|AE004798_15
                           (AE004798) type 4 fimbrial biogenesis protein PilF [Pseudomonas aeruginosa PAO1]
                           252
                           99.60
                           99.60
                           0
                           1263.0
     

     
     
146148
                           gb|AAB40949.1|
                           (L49434) homologous to HI0366 in Haemophilus influenzae [Pseudomonas aeruginosa]
                           252
                           77.38
                           77.38
                           54
                           978.0
     

     
     
146149
                           gb|AAO54953.1|
                           (AE016860) type IV pilus biogenesis protein PilF [Pseudomonas syringae pv. tomato str. DC3000]
                           252
                           63.09
                           82.14
                           0
                           839.0
     

     
     
146150
                           gb|AAN66476.1|AE016276_11
                           (AE016777) type IV pili biogenesis protein PilF [Pseudomonas putida KT2440]
                           255
                           55.68
                           70.58
                           3
                           701.0
     

     






      

          
GC ORFID: 46244How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3805Glimmer Score: 

          
Start: 3692735Stop: 3691977

          
Length: 759


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3805 translation of PA3805Homolog Bit Score 491.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4526 (ORF: BLASTX 317.0), t_PA3805 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTGTACGCGCCGCGCTGGTGTTCCTGTTGGCAGTTGGGCTGACAGGCTGCGTGACGAGCGGAGATCAG 
AATCCCCTGAAAACCGACAAGGGCCGTGACGAGGCGCGCGATGCCTATATCCAGCTCGGCCTCGGCTATCTC 
CAGCGGGGGAACACCGAGCAGGCCAAGGTGCCGCTGCGCAAGGCCCTCGAGATCGACCCTTCCAGCGCCGAC 
GCGCATGCCGCGCTGGCGGTGGTGTTCCAGACCGAGATGGAGCCCAAGCTGGCCGACGAGGAGTACCGCAAG 
GCGCTCGCCAGCGATAGCCGCAATGCCCGGGTACTGAACAACTACGGCGGTTTCCTCTACGAGCAGAAGCGC 
TACGAGGAGGCCTACCAGCGTCTCCTGGAGGCCAGCCAGGACACCCTCTATCCCGAGCGCTCGCGGGTCTTC 
GAGAACCTCGGCCTGGTTTCGCTGCAAATGAAGAAACCGGCCCAGGCCAAGGAGTACTTCGAGAAATCCCTG 
CGTCTGAACCGTAACCAGCCCAGCGTCGCGCTGGAGATGGCCGACCTGCTCTACAAGGAGCGCGAGTACGTG 
CCGGCTCGCCAGTACTATGATCTCTTTGCCCAGGGCGGCGGGCAGAACGCCCGCAGCCTGTTGCTGGGCATT 
CGTCTCGCGAAGGTCTTCGAGGACCGCGACACGGCCGCCAGCTATGGCCTGCAGTTGAAACGCCTGTACCCA 
GGCTCTCCCGAATATCAGGAATTCCAGGCGGAAAAATGA