ViewGene
Locus Name:
GeneID:
Location:

GeneID:3239
TrivialName: PA14_45740
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45740
PAO1 Ortholog Locus:PA1448
Sequence Length:777
Protein Length:258
Start: 4068673
Stop: 4067897
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04837.1|AE004574_8 (AE004574) flagellar biosynthetic protein FliR [Pseudomonas aeruginosa PAO1]
Identities = 257/258 (99%), Positives = 257/258 (99%)
Sequence:
ATGCTGGAGCTGACCAACGCGCAGATCGGCGGCTGGATCGCCAGCTTCGTGCTGCCGCTGTTCCGGGTCGCG
GCGCTGCTGATGACCATGCCGGTGATCGGCACCCAGCTGGTGCCGGTGCGGGTGCGCCTGTACCTGGCGCTG
GGCGTCTGCGTGGTGCTGGTGCCGAACCTGCCGCCGATGCCGCAGGTGGACGCGCTGAGCATGAAGGCCATG
CTGCTGATCGGCGAGCAGATCCTGGTCGGCGCCTTGCTCGGCTTTTCCCTGCAGTTGCTGTTCCACGCCTTC
GTGATCGCCGGGCAGATCATCTCGATGCAGATGGGCCTGGGCTTCGCTTCCATGGTCGACCCGGCCAACGGC
GTCTCGGTGCCGGTGCTCGGCCAGTTCTTCACCATGCTGGTGACGCTGCTGTTCCTGGCGATGAACGGCCAC
CTGGTAGTGTTCGAGGTGATCGCCGAGAGCTTCGTCACCCTGCCGGTGGGCGAGGGCCTGAGCGGCAACCAT
TTCTGGATCATCGCCGGCAAGCTCGGCTGGGTGATGGGCGCGGCACTGCTGCTGGCGCTGCCGGCGATCACC
GCGCTGCTGGTGGTGAACCTCGCCTTCGGCGCCATGACCCGCGCGGCGCCGCAACTGAACATCTTCTCCATC
GGCTTTCCGTTGACCCTGGTGCTGGGCCTGGTGATCCTCTGGATCGGCACCGCCGACCTGCTCAGCCAGTAC
CAGGTGCTGGCCGGCGAGGCCCTGCAATTCCTCCGTGAACTGGTGCGGGCGAAATAG
Translation:
MLELTNAQIGGWIASFVLPLFRVAALLMTMPVIGTQLVPVRVRLYLALGVCVVLVPNLPPMPQVDALSMKAM
LLIGEQILVGALLGFSLQLLFHAFVIAGQIISMQMGLGFASMVDPANGVSVPVLGQFFTMLVTLLFLAMNGH
LVVFEVIAESFVTLPVGEGLSGNHFWIIAGKLGWVMGAALLLALPAITALLVVNLAFGAMTRAAPQLNIFSI
GFPLTLVLGLVILWIGTADLLSQYQVLAGEALQFLRELVRAK*
AnnotationID:3247GeneID:3239
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 10:10:32
Gene Name:fliR
Confidence Code:2
GeneProduct:flagellar biosynthetic protein FliR
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1684,pfam01311
Secondary Functional Category(ies):chemotaxis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69931.1|AE016632_2 (AE016790) flagellar biosynthetic protein FliR [Pseudomonas putida
           KT2440]
          Length = 258

 Score =  367 bits (942), Expect = e-101
 Identities = 176/258 (68%), Positives = 215/258 (83%)

>gb|AAO55492.1| (AE016862) flagellar biosynthetic protein FliR [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 258

 Score =  355 bits (912), Expect = 1e-97
 Identities = 175/258 (67%), Positives = 210/258 (81%)

>gb|AAN56215.1|AE015759_7 (AE015759) flagellar biosynthetic protein FliR [Shewanella
           oneidensis MR-1]
          Length = 265

 Score =  224 bits (571), Expect = 4e-58
 Identities = 112/244 (45%), Positives = 165/244 (67%), Gaps = 1/244 (0%)






 
Structural Features:
COG1684, FliR, Flagellar biosynthesis pathway, component FliR 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 258 residues,  98.8% aligned
Score =  187 bits (476), Expect = 1e-48
Identities = 115/256 (44%), Positives = 161/256 (62%), Gaps = 2/256 (0%)

pfam01311, Bac_export_1, Bacterial export proteins, family 1.
CD-Length = 249 residues,  98.0% aligned
Score =  129 bits (327), Expect = 2e-31
Identities = 81/244 (33%), Positives = 128/244 (52%), Gaps = 3/244 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6481




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23623




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37382




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3239_cdd.html


    		




 
URL:

















    ReferenceID:37973




  
Author/Investigator(s):
        Malakooti J, Ely B, Matsumura P.





  
Title:
        Molecular characterization, nucleotide sequence, and expression of the fliO, fliP, fliQ, and fliR genes of Escherichia coli.




  
PubMed:
        8282695


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(1):189-97.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282695

















    ReferenceID:37980




  
Author/Investigator(s):
        Ohnishi K, Fan F, Schoenhals GJ, Kihara M, Macnab RM.





  
Title:
        The FliO, FliP, FliQ, and FliR proteins of Salmonella typhimurium: putative components for flagellar assembly.




  
PubMed:
        9324257


MedLine:
        




    
Source:
J Bacteriol. 1997 Oct;179(19):6092-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9324257

















    ReferenceID:37982




  
Author/Investigator(s):
        Zhuang WY, Shapiro L.





  
Title:
        Caulobacter FliQ and FliR membrane proteins, required for flagellar biogenesis and cell division, belong to a family of virulence factor export proteins.




  
PubMed:
        7814323


MedLine:
        




    
Source:
J Bacteriol. 1995 Jan;177(2):343-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7814323









           
Homologs By Global Alignment 

           

       



     
Gene ID:3239
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5213
                           PA1448_tr
                           translation of PA1448
                           258
                           99.61
                           99.61
                           0
                           1265.0
     

     
     
146108
                           gb|AAG04837.1|AE004574_8
                           (AE004574) flagellar biosynthetic protein FliR [Pseudomonas aeruginosa PAO1]
                           258
                           99.61
                           99.61
                           0
                           1265.0
     

     
     
146109
                           gb|AAN69931.1|AE016632_2
                           (AE016790) flagellar biosynthetic protein FliR [Pseudomonas putida KT2440]
                           258
                           68.21
                           83.33
                           0
                           942.0
     

     
     
146110
                           gb|AAD01928.2|
                           (AF031418) FliR [Pseudomonas putida]
                           258
                           68.21
                           83.72
                           0
                           933.0
     

     
     
146111
                           gb|AAO55492.1|
                           (AE016862) flagellar biosynthetic protein FliR [Pseudomonas syringae pv. tomato str. DC3000]
                           258
                           67.82
                           81.39
                           0
                           912.0
     

     






      

          
GC ORFID: 46241How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1448Glimmer Score: 

          
Start: 885236Stop: 886012

          
Length: 777


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA1448 translation of PA1448Homolog Bit Score 491.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7933 (ORF: BLASTX 360.0), t_PA1448 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGGAGCTGACCAACGCGCAGATCGGCGGCTGGATCGCCAGCTTCGTGCTGCCGCTGTTCCGGGTCGCG 
GCGCTGCTGATGACCATGCCGGTGATCGGCACCCAGCTGGTGCCGGTGCGGGTGCGCCTGTACCTGGCGCTG 
GGCGTCTGCGTGGTGCTGGTGCCGAACCTGCCGCCGATGCCGCAGGTGGACGCGCTGAGCATGAAGGCCATG 
CTGCTGATCGGCGAGCAGATCCTGGTCGGCGCCTTGCTCGGCTTTTCCCTGCAGTTGCTGTTCCACGCCTTC 
GTGATCGCCGGGCAGATCATCTCGATGCAGATGGGCCTGGGCTTCGCTTCCATGGTCGACCCGGCCAACGGC 
GTCTCGGTGCCGGTGCTCGGCCAGTTCTTCACCATGCTGGTGACGCTGCTGTTCCTGGCGATGAACGGCCAC 
CTGGTAGTGTTCGAGGTGATCGCCGAGAGCTTCGTCACCCTGCCGGTGGGCGAGGGCCTGAGCGGCAACCAT 
TTCTGGATCATCGCCGGCAAGCTCGGCTGGGTGATGGGCGCGGCACTGCTGCTGGCGCTGCCGGCGATCACC 
GCGCTGCTGGTGGTGAACCTCGCCTTCGGCGCCATGACCCGCGCGGCGCCGCAACTGAACATCTTCTCCATC 
GGCTTTCCGTTGACCCTGGTGCTGGGCCTGGTGATCCTCTGGATCGGCACCGCCGACCTGCTCAGCCAGTAC 
CAGGTGCTGGCCGGCGAGGCCCTGCAATTCCTCCGTGAACTGGTGCGGGCGAAATAG