ViewGene
Locus Name:
GeneID:
Location:

GeneID:3221
TrivialName: PA14_26510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_26510
PAO1 Ortholog Locus:PA2904
Sequence Length:753
Protein Length:250
Start: 2310252
Stop: 2311004
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06292.1|AE004716_10 (AE004716) precorrin-2 methyltransferase CobI [Pseudomonas aeruginosa PAO1]
Identities = 250/250 (100%), Positives = 250/250 (100%)
Sequence:
ATGATGAGTGTCGTGAAGGGCCGCCTGCTGGGCCTGGGCGTCGGGCCCGGCGATCCCGAACTGATCACCCTC
AAGGCCCTGCGCCTGCTGCGCGCGGCGCCGGTGGTCGGCTATTTCGTGGCCAAGGGCAAGAAGGGCAACGCC
TTCGGCATCATCGAGGCGCACCTGGACCAGGCCCAGGTGCGCCTGCCGCTGGTCTATCCGGTGACCACCGAG
AAGCTGGAACCGCCGCTGTGCTACGAGACCATCATCAGCGATTTCTACGACACCGCCGCCGAACAGCTGGCC
GCGCACCTGGACGCCGGCCGCGACGTGGCGGTGATCTGCGAGGGCGACCCGTTCTTCTACGGTTCCTACATG
TACCTCCACGACCGCCTGGCGACGCGCTACGAAAGCGAAGTGGTGCCTGGCGTTTGCTCGATGCTCGGCGGC
GCGGCGGTGCTCGGTGCGCCGCTGGTGTACCGCAACCAGAGCCTCTCGGTGCTCTCCGGAGTGCTGCCCGAG
GAGGAGTTGCGCCGGCGCCTGGCGGATGCCGACGCGGCGGTGGTGATGAAGCTCGGCCGCAATTTCGACAAG
GTCCGCCGCGTGCTGGTCGAGCTTGGCCTCGAGCGCCGCGCGCTGTACGTCGAGCGGGCGACCATGGCCAAC
CAGCGGATCGTGCCGTTGGAACGGGTGGAGCCGATGGCCTCGCCGTACTTCTCGCTGATCGTCGTCCCCGGC
GACAAGTGGCAGGGAGGGGCCGGCGGTGAGTGA
Translation:
MMSVVKGRLLGLGVGPGDPELITLKALRLLRAAPVVGYFVAKGKKGNAFGIIEAHLDQAQVRLPLVYPVTTE
KLEPPLCYETIISDFYDTAAEQLAAHLDAGRDVAVICEGDPFFYGSYMYLHDRLATRYESEVVPGVCSMLGG
AAVLGAPLVYRNQSLSVLSGVLPEEELRRRLADADAAVVMKLGRNFDKVRRVLVELGLERRALYVERATMAN
QRIVPLERVEPMASPYFSLIVVPGDKWQGGAGGE*
AnnotationID:3229GeneID:3221
AnnotatorUID: diggins
Modification Date/Time: 2005-04-07 18:06:25
Gene Name:cobI
Confidence Code:2
GeneProduct:precorrin-2 methyltransferase CobI
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:precorrin-2 C20-methyltransferase
Functional Category Confidence Code:5
COGs:COG2243
Secondary Functional Category(ies):
EC Number:2.1.1.130
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70396.1|AE016681_5 (AE016792) precorrin-2 C20-methyltransferase [Pseudomonas putida
           KT2440]
          Length = 244

 Score =  409 bits (1050), Expect = e-113
 Identities = 201/244 (82%), Positives = 221/244 (90%)

>gb|AAO58304.1| (AE016873) precorrin-2 C20-methyltransferase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 243

 Score =  397 bits (1020), Expect = e-110
 Identities = 191/240 (79%), Positives = 218/240 (90%)

>gb|AAL51894.1| (AE009513) PRECORRIN-2 C20-METHYLTRANSFERASE [Brucella melitensis
           16M]
          Length = 244

 Score =  337 bits (865), Expect = 3e-92
 Identities = 167/240 (69%), Positives = 194/240 (80%)






 
Structural Features:
COG2243, CobF, Precorrin-2 methylase [Coenzyme metabolism]
CD-Length = 234 residues, 100.0% aligned
Score =  270 bits (692), Expect = 1e-73
Identities = 120/241 (49%), Positives = 155/241 (64%), Gaps = 8/241 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6445




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23587




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36138




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3221_cdd.html


    		




 
URL:

















    ReferenceID:36412




  
Author/Investigator(s):
        Crouzet J, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Thibaut D, Debussche L.





  
Title:
        Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid.




  
PubMed:
        2211521


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5980-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211521









           
Homologs By Global Alignment 

           

       



     
Gene ID:3221
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5192
                           PA2904_tr
                           translation of PA2904
                           250
                           99.99
                           99.99
                           0
                           1272.0
     

     
     
145806
                           gb|AAG06292.1|AE004716_10
                           (AE004716) precorrin-2 methyltransferase CobI [Pseudomonas aeruginosa PAO1]
                           250
                           99.99
                           99.99
                           0
                           1272.0
     

     
     
145807
                           gb|AAN70396.1|AE016681_5
                           (AE016792) precorrin-2 C20-methyltransferase [Pseudomonas putida KT2440]
                           250
                           80.40
                           88.40
                           6
                           1050.0
     

     
     
145808
                           gb|AAO58304.1|
                           (AE016873) precorrin-2 C20-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           250
                           76.40
                           87.20
                           7
                           1017.0
     

     
     
145809
                           gb|AAN75031.1|
                           (AY150801) CobI [Rhodospirillum rubrum]
                           250
                           68.40
                           80.00
                           6
                           904.0
     

     






      

          
GC ORFID: 46223How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2904Glimmer Score: 

          
Start: 2643674Stop: 2642922

          
Length: 753


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2904 translation of PA2904Homolog Bit Score 494.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5298 (ORF: BLASTX 394.0), t_PA2904 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATGAGTGTCGTGAAGGGCCGCCTGCTGGGCCTGGGCGTCGGGCCCGGCGATCCCGAACTGATCACCCTC 
AAGGCCCTGCGCCTGCTGCGCGCGGCGCCGGTGGTCGGCTATTTCGTGGCCAAGGGCAAGAAGGGCAACGCC 
TTCGGCATCATCGAGGCGCACCTGGACCAGGCCCAGGTGCGCCTGCCGCTGGTCTATCCGGTGACCACCGAG 
AAGCTGGAACCGCCGCTGTGCTACGAGACCATCATCAGCGATTTCTACGACACCGCCGCCGAACAGCTGGCC 
GCGCACCTGGACGCCGGCCGCGACGTGGCGGTGATCTGCGAGGGCGACCCGTTCTTCTACGGTTCCTACATG 
TACCTCCACGACCGCCTGGCGACGCGCTACGAAAGCGAAGTGGTGCCTGGCGTTTGCTCGATGCTCGGCGGC 
GCGGCGGTGCTCGGTGCGCCGCTGGTGTACCGCAACCAGAGCCTCTCGGTGCTCTCCGGAGTGCTGCCCGAG 
GAGGAGTTGCGCCGGCGCCTGGCGGATGCCGACGCGGCGGTGGTGATGAAGCTCGGCCGCAATTTCGACAAG 
GTCCGCCGCGTGCTGGTCGAGCTTGGCCTCGAGCGCCGCGCGCTGTACGTCGAGCGGGCGACCATGGCCAAC 
CAGCGGATCGTGCCGTTGGAACGGGTGGAGCCGATGGCCTCGCCGTACTTCTCGCTGATCGTCGTCCCCGGC 
GACAAGTGGCAGGGAGGGGCCGGCGGTGAGTGA