ViewGene
Locus Name:
GeneID:
Location:

GeneID:3209
TrivialName: PA14_40900
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_40900
PAO1 Ortholog Locus:PA1827
Sequence Length:762
Protein Length:253
Start: 3648611
Stop: 3647850
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05216.1|AE004609_3 (AE004609) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 252/253 (99%), Positives = 252/253 (99%)
Sequence:
ATGCACACTCATTCTCCAATCCCCCGTACCGCCCTGGTCACCGGCGGCTCGTCGGGCATCGGCCTGGCCATC
GCCCGGCGCCTTCGGCAACAGGGTTATCGGATCGCCCTGGTGGCCCGTGATTCCCAGCGCCTGCAACGCGCT
GCCGCCGAGTTGGGCGGTGTGCCCTGGATGGCCGCCGATCTTTCCCGCCGCGAAGCTGTCGAGGCGGTGGCC
GCCTGGTTTGGCGACACCTTCCAGGGGCTCGACGTGCTGGTGAACAACGCCGGGTTCACCCGAAAGATCGCC
GCCACCACTCCGCTGGCTGAGGCCGAGCGGGAATGGGATGCGCTGCTGGACGGCAACCTGAAAAGCGCGTTC
CTCATGTCGCTGGCGGTGTTGCCGCATTTCGCGGAAGAGGGCGGGCGGATCATCCATATCGGATCGATCGCT
GCGCAGACCGGTAGCGGCAGTCCCGGTGCGCTGGGCTATGCGGCAGCCAAGGCCGGCCTGCATGGCTTCGGC
GTGGCCCTGGCGCGGGAACTCGGTTCGCGCGGGATCACGGTGAACAGCGTGGCACCGGGTTACATCGCCGAC
ACCCGGTTCTTTCCCGGCGGCCTCGATCCGCAACGGGTCGCCGCCATCGTCGGCGAGACCCCGCTGGGCAGG
ACCGGCCGGGTCGAGGACATCGCCGCGGCGGTGGCCTGGCTGGCCTCGCCCGAGGCGGGGTTCGTCACCGGA
ACGGTGGTGTCGGTGAACGGTGGCTGGCGGGTCGGCAACTGA
Translation:
MHTHSPIPRTALVTGGSSGIGLAIARRLRQQGYRIALVARDSQRLQRAAAELGGVPWMAADLSRREAVEAVA
AWFGDTFQGLDVLVNNAGFTRKIAATTPLAEAEREWDALLDGNLKSAFLMSLAVLPHFAEEGGRIIHIGSIA
AQTGSGSPGALGYAAAKAGLHGFGVALARELGSRGITVNSVAPGYIADTRFFPGGLDPQRVAAIVGETPLGR
TGRVEDIAAAVAWLASPEAGFVTGTVVSVNGGWRVGN*
AnnotationID:3217GeneID:3209
AnnotatorUID: diggins
Modification Date/Time: 2005-03-01 20:08:00
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA1827
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00106,COG1028,COG0300
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAC73847.1| (AP005045) putative 3-oxacyl-ACP reductase [Streptomyces
           avermitilis MA-4680]
          Length = 246

 Score =  171 bits (434), Expect = 3e-42
 Identities = 107/249 (42%), Positives = 141/249 (56%), Gaps = 9/249 (3%)

>gb|AAL52658.1| (AE009584) SHORT-CHAIN DEHYDROGENASE [Brucella melitensis 16M]
          Length = 245

 Score =  165 bits (418), Expect = 2e-40
 Identities = 106/242 (43%), Positives = 145/242 (59%), Gaps = 9/242 (3%)








 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase.
CD-Length = 247 residues, 100.0% aligned
Score =  160 bits (405), Expect = 2e-40
Identities = 100/252 (39%), Positives = 143/252 (56%), Gaps = 18/252 (7%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  98.0% aligned
Score =  148 bits (373), Expect = 8e-37
Identities = 104/252 (41%), Positives = 139/252 (55%), Gaps = 16/252 (6%)

COG4221, COG4221, Short-chain alcohol dehydrogenase of unknown specificity
CD-Length = 246 residues,  90.2% aligned
Score =  139 bits (353), Expect = 2e-34
Identities = 88/232 (37%), Positives = 122/232 (52%), Gaps = 17/232 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6421




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23563




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37109




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3209_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3209
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5176
                           PA1827_tr
                           translation of PA1827
                           253
                           99.60
                           99.60
                           0
                           1277.0
     

     
     
145476
                           gb|AAG05216.1|AE004609_3
                           (AE004609) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           253
                           99.60
                           99.60
                           0
                           1277.0
     

     
     
145480
                           emb|CAD14754.1|
                           (AL646062) PROBABLE 3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE OXIDOREDUCTASE [Ralstonia solanacearum]
                           263
                           44.48
                           57.03
                           24
                           435.0
     

     
     
145484
                           gb|AAN67532.1|AE016381_9
                           (AE016781) 3-oxoacyl-(acyl-carrier-protein) reductase [Pseudomonas putida KT2440]
                           260
                           43.07
                           55.38
                           21
                           409.0
     

     
     
145489
                           gb|AAF11496.1|AE002033_4
                           (AE002033) 3-oxoacyl-acyl carrier protein reductase [Deinococcus radiodurans] [Deinococcus radiodurans R1]
                           269
                           42.00
                           54.27
                           31
                           417.0
     

     






      

          
GC ORFID: 46211How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1827Glimmer Score: 

          
Start: 1305299Stop: 1306060

          
Length: 762


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1827 translation of PA1827Homolog Bit Score 496.0

          
Other Homologs:  t_PA5524 (ORF: BLASTX 161.0), t_PA2967 (ORF: BLASTX 140.0), t_PA1827 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCACACTCATTCTCCAATCCCCCGTACCGCCCTGGTCACCGGCGGCTCGTCGGGCATCGGCCTGGCCATC 
GCCCGGCGCCTTCGGCAACAGGGTTATCGGATCGCCCTGGTGGCCCGTGATTCCCAGCGCCTGCAACGCGCT 
GCCGCCGAGTTGGGCGGTGTGCCCTGGATGGCCGCCGATCTTTCCCGCCGCGAAGCTGTCGAGGCGGTGGCC 
GCCTGGTTTGGCGACACCTTCCAGGGGCTCGACGTGCTGGTGAACAACGCCGGGTTCACCCGAAAGATCGCC 
GCCACCACTCCGCTGGCTGAGGCCGAGCGGGAATGGGATGCGCTGCTGGACGGCAACCTGAAAAGCGCGTTC 
CTCATGTCGCTGGCGGTGTTGCCGCATTTCGCGGAAGAGGGCGGGCGGATCATCCATATCGGATCGATCGCT 
GCGCAGACCGGTAGCGGCAGTCCCGGTGCGCTGGGCTATGCGGCAGCCAAGGCCGGCCTGCATGGCTTCGGC 
GTGGCCCTGGCGCGGGAACTCGGTTCGCGCGGGATCACGGTGAACAGCGTGGCACCGGGTTACATCGCCGAC 
ACCCGGTTCTTTCCCGGCGGCCTCGATCCGCAACGGGTCGCCGCCATCGTCGGCGAGACCCCGCTGGGCAGG 
ACCGGCCGGGTCGAGGACATCGCCGCGGCGGTGGCCTGGCTGGCCTCGCCCGAGGCGGGGTTCGTCACCGGA 
ACGGTGGTGTCGGTGAACGGTGGCTGGCGGGTCGGCAACTGA