ViewGene
Locus Name:
GeneID:
Location:

GeneID:3201
TrivialName: PA14_25920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25920
PAO1 Ortholog Locus:PA2948
Sequence Length:753
Protein Length:250
Start: 2261851
Stop: 2261099
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06336.1|AE004721_4 (AE004721) precorrin-3 methylase [Pseudomonas aeruginosa PAO1]
Identities = 250/250 (100%), Positives = 250/250 (100%)
Sequence:
ATGACCGTCTATTTCATCGGCGCCGGTCCCGGCGACCCTGACCTGATCACCGTCAAGGGGCAGCGCCTGATC
CGCCAGTGCCCGGTGATCCTCTACGCCGGCTCGCTGGTTCCGCAAGCGCTGCTCGAGGGGCACCAGGCCGGC
CAGGTGGTGAACACCGCCGAGCTGGACCTCGAACAGATCGTCGAGCTACTCGCGCAAGCCCATCGCCGCGGC
CTCGACGTAGCCCGCGTGCATTCCGGCGATCCCTCCCTGTACGGCGCCATCGGCGAGCAGATCCGCCACCTG
CGCGAACTCGGCATCCCCTATGAGATCGTCCCCGGAGTCACCGCCACCGCCGCCTGCGCCGCCCTGCTCGGC
TGCGAGCTGACCCTACCCGAAGTGTCCCAGACGCTGATCCTGACCCGCTACGCCGCACGCACGAAGATGCCG
GAAGGCGAGAGCCTGGGCGACCTGGCGCGGCACCGGGCGACCCTGGCGATCCACCTGGGCGTCGCCCACCTG
GCGAAGATCGTCGAGGAACTGCTGCCGCACTACGGCGCCGACTGCCCGGTCGCGGTGATCCACCGGGCCAGT
TGGCCCGACCAGGAACAGGTTCGCGGCACCCTCGGCGACATCCTGCCCAAGGTCGCCGCCAGGAATTTCCGC
CGCACCGCGCTGATCCTGGTCGGCGAGGTGCTGGCCGCCGAAGGCTTCGCCGACTCTTCGCTGTACCGCGCC
GAGCATCGACATCTCTATCGACCCGGCGAATGA
Translation:
MTVYFIGAGPGDPDLITVKGQRLIRQCPVILYAGSLVPQALLEGHQAGQVVNTAELDLEQIVELLAQAHRRG
LDVARVHSGDPSLYGAIGEQIRHLRELGIPYEIVPGVTATAACAALLGCELTLPEVSQTLILTRYAARTKMP
EGESLGDLARHRATLAIHLGVAHLAKIVEELLPHYGADCPVAVIHRASWPDQEQVRGTLGDILPKVAARNFR
RTALILVGEVLAAEGFADSSLYRAEHRHLYRPGE*
AnnotationID:3209GeneID:3201
AnnotatorUID: diggins
Modification Date/Time: 2005-04-08 17:05:16
Gene Name:cobM
Confidence Code:2
GeneProduct:precorrin-3 methylase
Cell Localization:()
Synonyms:cbiF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:precorrin-4 C11-methyltransferase
Functional Category Confidence Code:5
COGs:COG2875
Secondary Functional Category(ies):
EC Number:2.1.1.133
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69012.1|AE016533_4 (AE016786) precorrin-4 C11-methyltransferase [Pseudomonas putida
           KT2440]
          Length = 250

 Score =  384 bits (985), Expect = e-106
 Identities = 186/250 (74%), Positives = 216/250 (86%)

>gb|AAO56640.1| (AE016867) precorrin-4 C11-methyltransferase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 249

 Score =  382 bits (980), Expect = e-105
 Identities = 188/248 (75%), Positives = 212/248 (85%)

>gb|AAC16190.1| (AF010496) precorrin-3 methylase [Rhodobacter capsulatus]
          Length = 261

 Score =  263 bits (673), Expect = 5e-70
 Identities = 139/250 (55%), Positives = 179/250 (71%), Gaps = 3/250 (1%)








 
Structural Features:
COG2875, CobM, Precorrin-4 methylase [Coenzyme metabolism]
CD-Length = 254 residues,  98.4% aligned
Score =  367 bits (944), Expect = 7e-103
Identities = 150/252 (59%), Positives = 186/252 (73%), Gaps = 2/252 (0%)








   
Genomic Context:






   
Comment:


















    ReferenceID:6405




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23547




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36095




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3201_cdd.html


    		




 
URL:

















    ReferenceID:36427




  
Author/Investigator(s):
        Crouzet J, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Thibaut D, Debussche L.





  
Title:
        Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid.




  
PubMed:
        2211521


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5980-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2211521









           
Homologs By Global Alignment 

           

       



     
Gene ID:3201
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5160
                           PA2948_tr
                           translation of PA2948
                           250
                           99.99
                           99.99
                           0
                           1279.0
     

     
     
145037
                           gb|AAG06336.1|AE004721_4
                           (AE004721) precorrin-3 methylase [Pseudomonas aeruginosa PAO1]
                           250
                           99.99
                           99.99
                           0
                           1279.0
     

     
     
145039
                           gb|AAO56640.1|
                           (AE016867) precorrin-4 C11-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           250
                           75.20
                           84.80
                           1
                           978.0
     

     
     
145038
                           gb|AAN69012.1|AE016533_4
                           (AE016786) precorrin-4 C11-methyltransferase [Pseudomonas putida KT2440]
                           250
                           74.40
                           86.40
                           0
                           985.0
     

     
     
145051
                           emb|CAD96950.1|
                           (BX248341) Probable precorrin-4 C11-methyltransferase CobM [Mycobacterium bovis subsp. bovis AF2122/97]
                           253
                           55.73
                           64.82
                           5
                           626.0
     

     






      

          
GC ORFID: 46203How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2948Glimmer Score: 

          
Start: 2692074Stop: 2692826

          
Length: 753


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2948 translation of PA2948Homolog Bit Score 497.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7240 (ORF: BLASTX 384.0), t_PA2948 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGTCTATTTCATCGGCGCCGGTCCCGGCGACCCTGACCTGATCACCGTCAAGGGGCAGCGCCTGATC 
CGCCAGTGCCCGGTGATCCTCTACGCCGGCTCGCTGGTTCCGCAAGCGCTGCTCGAGGGGCACCAGGCCGGC 
CAGGTGGTGAACACCGCCGAGCTGGACCTCGAACAGATCGTCGAGCTACTCGCGCAAGCCCATCGCCGCGGC 
CTCGACGTAGCCCGCGTGCATTCCGGCGATCCCTCCCTGTACGGCGCCATCGGCGAGCAGATCCGCCACCTG 
CGCGAACTCGGCATCCCCTATGAGATCGTCCCCGGAGTCACCGCCACCGCCGCCTGCGCCGCCCTGCTCGGC 
TGCGAGCTGACCCTACCCGAAGTGTCCCAGACGCTGATCCTGACCCGCTACGCCGCACGCACGAAGATGCCG 
GAAGGCGAGAGCCTGGGCGACCTGGCGCGGCACCGGGCGACCCTGGCGATCCACCTGGGCGTCGCCCACCTG 
GCGAAGATCGTCGAGGAACTGCTGCCGCACTACGGCGCCGACTGCCCGGTCGCGGTGATCCACCGGGCCAGT 
TGGCCCGACCAGGAACAGGTTCGCGGCACCCTCGGCGACATCCTGCCCAAGGTCGCCGCCAGGAATTTCCGC 
CGCACCGCGCTGATCCTGGTCGGCGAGGTGCTGGCCGCCGAAGGCTTCGCCGACTCTTCGCTGTACCGCGCC 
GAGCATCGACATCTCTATCGACCCGGCGAATGA