ViewGene
Locus Name:
GeneID:
Location:

GeneID:320
TrivialName: PA14_07130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_07130
PAO1 Ortholog Locus:PA0548
Sequence Length:1998
Protein Length:665
Start: 616135
Stop: 618132
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03937.1|AE004491_4 (AE004491) transketolase [Pseudomonas aeruginosa PAO1]
Identities = 665/665 (100%), Positives = 665/665 (100%)
Sequence:
ATGCCCAGCCGTCGTGAGCGAGCCAATGCCATCCGTGCACTGAGCATGGATGCCGTGCAGAAAGCCAACAGC
GGCCACCCGGGCGCCCCGATGGGCATGGCCGATATCGCCGAGGTCCTCTGGCGCGACTACATGCAGCACAAC
CCGAGCAACCCGCAGTGGGCCAACCGCGACCGCTTCGTGCTGTCCAACGGCCACGGCTCGATGCTGATCTAC
TCCCTGCTGCACCTCACCGGGTACGACCTCGGCATCGAGGACCTGAAGAACTTCCGCCAGCTCAACTCGCGC
ACCCCGGGCCATCCGGAGTACGGCTACACCGCCGGCGTCGAGACCACCACCGGTCCGCTCGGCCAGGGCATC
GCCAATGCGGTGGGCATGGCGCTGGCGGAGAAGGTCCTGGCCGCCCAGTTCAACCGCGACGGCCACGCGGTG
GTCGACCACTACACCTACGCCTTCCTCGGCGACGGCTGCATGATGGAAGGCATTTCCCATGAGGTCGCCTCG
CTGGCCGGCACCCTGCGCCTGAACAAGCTGATCGCCTTCTACGACGACAACGGCATTTCCATCGACGGCGAG
GTCCACGGCTGGTTCACCGACGACACCCCGAAGCGCTTCGAGGCCTATGGCTGGCAAGTGATCCGCAACGTC
GACGGGCATGACGCCGACGAGATCAAGACCGCCATCGATACCGCGCGCAAGAGCGACCAGCCGACCCTGATC
TGCTGCAAGACCGTGATCGGCTTCGGCTCGCCGAACAAGCAGGGCAAGGAAGAGTGCCACGGCGCGCCGCTG
GGTGCCGACGAGATCGCCGCGACCCGCGCCGCGCTGGGCTGGGAGCACGCTCCGTTCGAGATCCCGGCGCAG
ATCTACGCCGAGTGGGACGCCAAGGAAACCGGCGCCGCCCAGGAAGCCGAGTGGAACAAGCGTTTCGCCGCC
TACCAGGCCGCCCATCCGGAACTGGCCGCCGAACTGCTGCGCCGCCTGAAGGGCGAGCTGCCGGCCGACTTC
GCCGAGAAGGCCGCGGCCTACGTCGCCGATGTTGCCAACAAGGGTGAGACCATCGCCAGCCGCAAGGCCAGC
CAGAACGCGCTGAACGCCTTCGGCCCGCTGCTGCCGGAGCTGCTCGGCGGTTCCGCCGACCTGGCCGGCTCC
AACCTGACCCTGTGGAAGGGCTGCAAGGGCGTCAGCGCCGACGACGCCGCCGGCAATTACGTGTTCTACGGC
GTGCGCGAATTCGGCATGAGCGCGATCATGAATGGCGTCGCCCTGCACGGCGGTTTCATTCCCTACGGTGCG
ACCTTCCTGATCTTCATGGAATACGCGCGCAACGCCGTGCGCATGTCCGCACTGATGAAGCAGCGCGTGCTC
TACGTGTTCACCCACGACTCCATCGGCCTCGGCGAGGACGGCCCGACCCACCAGCCGATCGAACAACTGGCC
AGCCTGCGCCTGACCCCGAACCTGGACACCTGGCGCCCGGCCGACGCGGTCGAGTCGGCGGTGGCCTGGAAG
CATGCCATCGAGCGTGCCGACGGTCCGTCCGCGCTGATCTTCTCCCGCCAGAACCTGCCGCACCAGGCGCGC
GACGTCGCCCAGGTGGCCGATATCGCCCGCGGCGGCTACGTGCTGAAGGACTGCGAAGGCGAGCCGGAACTG
ATCCTGATCGCCACCGGTTCGGAAGTCGGCCTGGCCGTGCAGGCCTACGACAAGCTCAGCGAGCAGGGCCGC
AAGGTCCGCGTGGTATCGATGCCGTGCACCAGCGTCTACGAGCAGCAGGACGAGTCCTACAAGCAGTCCGTG
CTGCCGGTGGAAGTCGGCGCGCGCATCGCCATCGAGGCCGCCCATGCCGACTACTGGTACAAGTACGTCGGT
CTCGACGGGCGCATCATCGGCATGACCAGCTTCGGCGAGTCGGCTCCGGCCCCGGCGCTGTTCGAGCACTTC
GGCTTCACCCTGGACAACGTCCTGGCGGTGGCCGAGGAGCTGCTGGAAGACTGA
Translation:
MPSRRERANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWRDYMQHNPSNPQWANRDRFVLSNGHGSMLIY
SLLHLTGYDLGIEDLKNFRQLNSRTPGHPEYGYTAGVETTTGPLGQGIANAVGMALAEKVLAAQFNRDGHAV
VDHYTYAFLGDGCMMEGISHEVASLAGTLRLNKLIAFYDDNGISIDGEVHGWFTDDTPKRFEAYGWQVIRNV
DGHDADEIKTAIDTARKSDQPTLICCKTVIGFGSPNKQGKEECHGAPLGADEIAATRAALGWEHAPFEIPAQ
IYAEWDAKETGAAQEAEWNKRFAAYQAAHPELAAELLRRLKGELPADFAEKAAAYVADVANKGETIASRKAS
QNALNAFGPLLPELLGGSADLAGSNLTLWKGCKGVSADDAAGNYVFYGVREFGMSAIMNGVALHGGFIPYGA
TFLIFMEYARNAVRMSALMKQRVLYVFTHDSIGLGEDGPTHQPIEQLASLRLTPNLDTWRPADAVESAVAWK
HAIERADGPSALIFSRQNLPHQARDVAQVADIARGGYVLKDCEGEPELILIATGSEVGLAVQAYDKLSEQGR
KVRVVSMPCTSVYEQQDESYKQSVLPVEVGARIAIEAAHADYWYKYVGLDGRIIGMTSFGESAPAPALFEHF
GFTLDNVLAVAEELLED*
AnnotationID:330GeneID:320
AnnotatorUID: diggins
Modification Date/Time: 2005-03-28 18:06:19
Gene Name:tktA
Confidence Code:2
GeneProduct:transketolase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:transketolase 1 isozyme
Functional Category Confidence Code:5
COGs:COG0021,pfam00456
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.2.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70532.1|AE016695_6 (AE016792) transketolase [Pseudomonas putida KT2440]
          Length = 665

 Score = 1177 bits (3045), Expect = 0.0
 Identities = 570/665 (85%), Positives = 614/665 (92%)

>gb|AAO09963.1|AE016802_6 (AE016802) Transketolase [Vibrio vulnificus CMCP6]
          Length = 670

 Score = 1010 bits (2611), Expect = 0.0
 Identities = 483/663 (72%), Positives = 561/663 (84%)

>dbj|BAB37233.1| (AP002563) transketolase 1 isozyme [Escherichia coli O157:H7]
          Length = 663

 Score = 1010 bits (2611), Expect = 0.0
 Identities = 486/664 (73%), Positives = 567/664 (85%), Gaps = 2/664 (0%)






 
Structural Features:
COG0021, TktA, Transketolase [Carbohydrate transport and metabolism]
CD-Length = 663 residues,  99.7% aligned
Score = 1057 bits (2735), Expect = 0.0
Identities = 429/667 (64%), Positives = 512/667 (76%), Gaps = 9/667 (1%)

pfam00456, Transketolase_N, Transketolase, thiamine diphosphate binding domain.
CD-Length = 333 residues, 100.0% aligned
Score =  525 bits (1353), Expect = 7e-150
Identities = 226/333 (67%), Positives = 267/333 (80%), Gaps = 2/333 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:643




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17785




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33857




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:320_cdd.html


    		




 
URL:

















    ReferenceID:35293




  
Author/Investigator(s):
        de Sury d'Aspremont R, Toussaint B, Vignais PM.





  
Title:
        Isolation of Rhodobacter capsulatus transketolase: cloning and sequencing of its structural tktA gene.




  
PubMed:
        8635754


MedLine:
        




    
Source:
Gene. 1996 Feb 22;169(1):81-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8635754

















    ReferenceID:35294




  
Author/Investigator(s):
        Iida A, Teshiba S, Mizobuchi K.





  
Title:
        Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli K-12.




  
PubMed:
        8396116


MedLine:
        




    
Source:
J Bacteriol. 1993 Sep;175(17):5375-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8396116









           
Homologs By Global Alignment 

           

       



     
Gene ID:320
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
529
                           PA0548_tr
                           translation of PA0548
                           665
                           99.99
                           99.99
                           0
                           3485.0
     

     
     
26070
                           gb|AAG03937.1|AE004491_4
                           (AE004491) transketolase [Pseudomonas aeruginosa PAO1]
                           665
                           99.99
                           99.99
                           0
                           3485.0
     

     
     
26071
                           gb|AAN70532.1|AE016695_6
                           (AE016792) transketolase [Pseudomonas putida KT2440]
                           665
                           85.71
                           92.33
                           0
                           3045.0
     

     
     
26072
                           gb|AAO53929.1|
                           (AE016857) transketolase [Pseudomonas syringae pv. tomato str. DC3000]
                           665
                           83.60
                           92.18
                           0
                           3005.0
     

     
     
26078
                           dbj|BAB37233.1|
                           (AP002563) transketolase 1 isozyme [Escherichia coli O157:H7]
                           666
                           72.97
                           85.13
                           4
                           2615.0
     

     






      

          
GC ORFID: 43322How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0548Glimmer Score: 

          
Start: 6417477Stop: 6419474

          
Length: 1998


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0548 translation of PA0548Homolog Bit Score 1347.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6571 (ORF: BLASTX 1157.0), t_PA0548 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCAGCCGTCGTGAGCGAGCCAATGCCATCCGTGCACTGAGCATGGATGCCGTGCAGAAAGCCAACAGC 
GGCCACCCGGGCGCCCCGATGGGCATGGCCGATATCGCCGAGGTCCTCTGGCGCGACTACATGCAGCACAAC 
CCGAGCAACCCGCAGTGGGCCAACCGCGACCGCTTCGTGCTGTCCAACGGCCACGGCTCGATGCTGATCTAC 
TCCCTGCTGCACCTCACCGGGTACGACCTCGGCATCGAGGACCTGAAGAACTTCCGCCAGCTCAACTCGCGC 
ACCCCGGGCCATCCGGAGTACGGCTACACCGCCGGCGTCGAGACCACCACCGGTCCGCTCGGCCAGGGCATC 
GCCAATGCGGTGGGCATGGCGCTGGCGGAGAAGGTCCTGGCCGCCCAGTTCAACCGCGACGGCCACGCGGTG 
GTCGACCACTACACCTACGCCTTCCTCGGCGACGGCTGCATGATGGAAGGCATTTCCCATGAGGTCGCCTCG 
CTGGCCGGCACCCTGCGCCTGAACAAGCTGATCGCCTTCTACGACGACAACGGCATTTCCATCGACGGCGAG 
GTCCACGGCTGGTTCACCGACGACACCCCGAAGCGCTTCGAGGCCTATGGCTGGCAAGTGATCCGCAACGTC 
GACGGGCATGACGCCGACGAGATCAAGACCGCCATCGATACCGCGCGCAAGAGCGACCAGCCGACCCTGATC 
TGCTGCAAGACCGTGATCGGCTTCGGCTCGCCGAACAAGCAGGGCAAGGAAGAGTGCCACGGCGCGCCGCTG 
GGTGCCGACGAGATCGCCGCGACCCGCGCCGCGCTGGGCTGGGAGCACGCTCCGTTCGAGATCCCGGCGCAG 
ATCTACGCCGAGTGGGACGCCAAGGAAACCGGCGCCGCCCAGGAAGCCGAGTGGAACAAGCGTTTCGCCGCC 
TACCAGGCCGCCCATCCGGAACTGGCCGCCGAACTGCTGCGCCGCCTGAAGGGCGAGCTGCCGGCCGACTTC 
GCCGAGAAGGCCGCGGCCTACGTCGCCGATGTTGCCAACAAGGGTGAGACCATCGCCAGCCGCAAGGCCAGC 
CAGAACGCGCTGAACGCCTTCGGCCCGCTGCTGCCGGAGCTGCTCGGCGGTTCCGCCGACCTGGCCGGCTCC 
AACCTGACCCTGTGGAAGGGCTGCAAGGGCGTCAGCGCCGACGACGCCGCCGGCAATTACGTGTTCTACGGC 
GTGCGCGAATTCGGCATGAGCGCGATCATGAATGGCGTCGCCCTGCACGGCGGTTTCATTCCCTACGGTGCG 
ACCTTCCTGATCTTCATGGAATACGCGCGCAACGCCGTGCGCATGTCCGCACTGATGAAGCAGCGCGTGCTC 
TACGTGTTCACCCACGACTCCATCGGCCTCGGCGAGGACGGCCCGACCCACCAGCCGATCGAACAACTGGCC 
AGCCTGCGCCTGACCCCGAACCTGGACACCTGGCGCCCGGCCGACGCGGTCGAGTCGGCGGTGGCCTGGAAG 
CATGCCATCGAGCGTGCCGACGGTCCGTCCGCGCTGATCTTCTCCCGCCAGAACCTGCCGCACCAGGCGCGC 
GACGTCGCCCAGGTGGCCGATATCGCCCGCGGCGGCTACGTGCTGAAGGACTGCGAAGGCGAGCCGGAACTG 
ATCCTGATCGCCACCGGTTCGGAAGTCGGCCTGGCCGTGCAGGCCTACGACAAGCTCAGCGAGCAGGGCCGC 
AAGGTCCGCGTGGTATCGATGCCGTGCACCAGCGTCTACGAGCAGCAGGACGAGTCCTACAAGCAGTCCGTG 
CTGCCGGTGGAAGTCGGCGCGCGCATCGCCATCGAGGCCGCCCATGCCGACTACTGGTACAAGTACGTCGGT 
CTCGACGGGCGCATCATCGGCATGACCAGCTTCGGCGAGTCGGCTCCGGCCCCGGCGCTGTTCGAGCACTTC 
GGCTTCACCCTGGACAACGTCCTGGCGGTGGCCGAGGAGCTGCTGGAAGACTGA