ViewGene
Locus Name:
GeneID:
Location:

GeneID:3179
TrivialName: PA14_37040
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37040
PAO1 Ortholog Locus:PA2129
Sequence Length:747
Protein Length:248
Start: 3300263
Stop: 3299517
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05517.1|AE004640_6 (AE004640) chaperone CupA2 [Pseudomonas aeruginosa PAO1]
Identities = 248/248 (100%), Positives = 248/248 (100%)
Sequence:
ATGCCCAGACATTCGTACCGATCCGGCAGGACATCGGCGCTTCTGCTCGTCCTGCTGGCGAGCATCGCCCAG
GCGCGGGCAAGCGTGGTGGTGACCGGGACCCGGGTGATCTATCCCGGCGAGGCACGGGAGAAGACCGTGCAA
CTGAGCAATCGCGATGCGTTTCCCAACGTCGTCCAGGCCTGGGTCGACATCGACGCCCCGGACGCGCCGCCG
GACCAGGCGGACGCGCCGTTCCTGGTCAACCCGGCGGTATTCCGCATGGCCCCGGACAGCGGCCAGACGCTG
CGCATCGTCTACACCGGGCAGGGCCTGCCGGGCGATCGCGAATCGCTGTTCCATCTCAACGTCCTGCAGATC
CCGCCACGCAACAGCAGCCATGCGGATCGGAACCAGATGCTGCTGATGCTGCGCAATCGCCTGAAGCTGTTC
TACCGCCCGGCGGGCATCCAGGGCCGTCCCGAGGACCTGCCCGGGCAACTGCGTTTCGCACTGGTCCGGCGC
AGCGCCGGCTGGGCGGTGCGGGTGGACAACCCGAGCGGCTACTACGCCTCGTTCGCCAGCGCCACGCTCAGC
GTCGGCCAGCGCCGCTGGCGCTTGCGCAGCGGCATGCTGGAGCCGCGCAGCCATGCCGAATGGCAGGCCGAA
ACCCGAGAAGCCCTGCCGCCGGGACGCGTGCGCCTGCACGCGCTGCTGATCAACGACTACGGCGCCCATATG
GATATCCGTCATGACCTATCGCCATGA
Translation:
MPRHSYRSGRTSALLLVLLASIAQARASVVVTGTRVIYPGEAREKTVQLSNRDAFPNVVQAWVDIDAPDAPP
DQADAPFLVNPAVFRMAPDSGQTLRIVYTGQGLPGDRESLFHLNVLQIPPRNSSHADRNQMLLMLRNRLKLF
YRPAGIQGRPEDLPGQLRFALVRRSAGWAVRVDNPSGYYASFASATLSVGQRRWRLRSGMLEPRSHAEWQAE
TREALPPGRVRLHALLINDYGAHMDIRHDLSP*
AnnotationID:3187GeneID:3179
AnnotatorUID: diggins
Modification Date/Time: 2005-03-03 10:10:06
Gene Name:cupA2
Confidence Code:1
GeneProduct:chaperone CupA2
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:fimbrial chaperone
Functional Category Confidence Code:5
COGs:COG3121,pfam00345
Secondary Functional Category(ies):motility and attachment
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM83941.1|AE013635_6 (AE013635) chaperone [Yersinia pestis KIM]
          Length = 251

 Score =  191 bits (484), Expect = 4e-48
 Identities = 97/222 (43%), Positives = 141/222 (63%), Gaps = 3/222 (1%)

>gb|AAG55652.1|AE005303_8 (AE005303) putative chaperone [Escherichia coli O157:H7 EDL933]
          Length = 240

 Score =  161 bits (408), Expect = 3e-39
 Identities = 91/238 (38%), Positives = 140/238 (58%), Gaps = 13/238 (5%)

>gb|AAO70108.1| (AE016842) fimbrial chaperone protein [Salmonella enterica subsp.
           enterica serovar Typhi Ty2]
          Length = 253

 Score =  158 bits (399), Expect = 3e-38
 Identities = 90/238 (37%), Positives = 141/238 (59%), Gaps = 6/238 (2%)








 
Structural Features:
COG3121, FimC, P pilus assembly protein, chaperone PapD 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 235 residues, 100.0% aligned
Score =  215 bits (549), Expect = 3e-57
Identities = 101/246 (41%), Positives = 139/246 (56%), Gaps = 11/246 (4%)

pfam00345, Pili_assembly_N, Gram-negative pili assembly chaperone, 
N-terminal domain.
CD-Length = 122 residues,  99.2% aligned
Score =  133 bits (336), Expect = 2e-32
Identities = 59/125 (47%), Positives = 81/125 (64%), Gaps = 6/125 (4%)









   
Genomic Context:






   
Comment:


















    ReferenceID:6361




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23503




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32594




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3179_cdd.html


    		




 
URL:

















    ReferenceID:34419




  
Author/Investigator(s):
        Vallet I, Olson JW, Lory S, Lazdunski A, Filloux A.





  
Title:
        The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation.




  
PubMed:
        11381121


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6911-6. Epub 2001 May 29.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11381121









           
Homologs By Global Alignment 

           

       



     
Gene ID:3179
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5112
                           PA2129_tr
                           translation of PA2129
                           248
                           99.99
                           99.99
                           0
                           1286.0
     

     
     
143307
                           gb|AAG05517.1|AE004640_6
                           (AE004640) chaperone CupA2 [Pseudomonas aeruginosa PAO1]
                           248
                           99.99
                           99.99
                           0
                           1286.0
     

     
     
143308
                           gb|AAP84170.1|
                           (AY273869) putative pili assembly chaperone [Pseudomonas aeruginosa]
                           248
                           62.09
                           75.00
                           0
                           782.0
     

     
     
143310
                           emb|CAC93345.1|
                           (AJ414159) putative fimbrial chaperone [Yersinia pestis CO92]
                           261
                           39.46
                           56.70
                           31
                           478.0
     

     
     
143309
                           gb|AAM83941.1|AE013635_6
                           (AE013635) chaperone [Yersinia pestis KIM]
                           269
                           38.28
                           55.01
                           39
                           478.0
     

     






      

          
GC ORFID: 46181How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2129Glimmer Score: 

          
Start: 1653651Stop: 1654397

          
Length: 747


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2129 translation of PA2129Homolog Bit Score 499.0

          
Other Homologs:  t_RL043 (ORF: BLASTX 310.0), t_PA2129 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCAGACATTCGTACCGATCCGGCAGGACATCGGCGCTTCTGCTCGTCCTGCTGGCGAGCATCGCCCAG 
GCGCGGGCAAGCGTGGTGGTGACCGGGACCCGGGTGATCTATCCCGGCGAGGCACGGGAGAAGACCGTGCAA 
CTGAGCAATCGCGATGCGTTTCCCAACGTCGTCCAGGCCTGGGTCGACATCGACGCCCCGGACGCGCCGCCG 
GACCAGGCGGACGCGCCGTTCCTGGTCAACCCGGCGGTATTCCGCATGGCCCCGGACAGCGGCCAGACGCTG 
CGCATCGTCTACACCGGGCAGGGCCTGCCGGGCGATCGCGAATCGCTGTTCCATCTCAACGTCCTGCAGATC 
CCGCCACGCAACAGCAGCCATGCGGATCGGAACCAGATGCTGCTGATGCTGCGCAATCGCCTGAAGCTGTTC 
TACCGCCCGGCGGGCATCCAGGGCCGTCCCGAGGACCTGCCCGGGCAACTGCGTTTCGCACTGGTCCGGCGC 
AGCGCCGGCTGGGCGGTGCGGGTGGACAACCCGAGCGGCTACTACGCCTCGTTCGCCAGCGCCACGCTCAGC 
GTCGGCCAGCGCCGCTGGCGCTTGCGCAGCGGCATGCTGGAGCCGCGCAGCCATGCCGAATGGCAGGCCGAA 
ACCCGAGAAGCCCTGCCGCCGGGACGCGTGCGCCTGCACGCGCTGCTGATCAACGACTACGGCGCCCATATG 
GATATCCGTCATGACCTATCGCCATGA