ViewGene
Locus Name:
GeneID:
Location:

GeneID:3166
TrivialName: PA14_20270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20270
PAO1 Ortholog Locus:PA3387
Sequence Length:771
Protein Length:256
Start: 1746985
Stop: 1746215
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06775.1|AE004760_3 (AE004760) beta-ketoacyl reductase [Pseudomonas aeruginosa PAO1]
Identities = 254/256 (99%), Positives = 255/256 (99%)
Sequence:
ATGCATCCCTATTTCAGCCTCGCCGGCCGCGTCGCCCTGGTCACCGGCGGCAGCCGCGGCATCGGCCAGATG
ATCGCCCAGGGTCTTCTCGAAGCCGGCGCCCGCGTCTTCATCTGCGCACGTGACGCAGAGGCCTGCGCCGAC
ACCGCCACACACCTCTCGGCCTACGGCGATTGCCAGGCGATCCCTGCCGACCTTTCCAGCGAAGCCGGTGCC
CGGCGCCTGGCGCAGGCGCTCGGCGAACTCAGCGCGCGGCTGGATATCCTGGTGAACAACGCCGGCACCAGT
TGGGGCGCAGCGCTGGAAAGCTATCCGGTGTCCGGCTGGGAAAAGGTCATGCAGCTCAATGTCACCTCGGTG
TTTTCCTGCATCCAGCAACTGCTGCCGCTGCTGCGCCGCTCGGCCAGCGCGGAAAATCCGGCGCGAGTGATC
AACATCGGCTCGGTGGCGGGCATCAGCGCCATGGGCGAACAAGCCTATGCCTACGGGCCGAGCAAGGCCGCG
CTGCATCAACTGTCGCGGATGCTGGCGAAGGAACTGGTCGGCGAGCACATCAATGTCAACGTGATCGCTCCC
GGACGCTTCCCGAGCCGCATGACCCGTCACATCGCCAATGACCCGCAGGCACTGGAGGCGGACAGCGCGTCG
ATTCCCATGGGCCGCTGGGGTCGCCCGGAGGAGATGGCGGCGCTGGCGATCAGCCTGGCCGGCACCGCCGGC
GCCTACATGACCGGCAACGTGATCCCCATCGACGGCGGTTTTCATCTCTGA
Translation:
MHPYFSLAGRVALVTGGSRGIGQMIAQGLLEAGARVFICARDAEACADTATHLSAYGDCQAIPADLSSEAGA
RRLAQALGELSARLDILVNNAGTSWGAALESYPVSGWEKVMQLNVTSVFSCIQQLLPLLRRSASAENPARVI
NIGSVAGISAMGEQAYAYGPSKAALHQLSRMLAKELVGEHINVNVIAPGRFPSRMTRHIANDPQALEADSAS
IPMGRWGRPEEMAALAISLAGTAGAYMTGNVIPIDGGFHL*
AnnotationID:3174GeneID:3166
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 08:08:07
Gene Name:rhlG
Confidence Code:1
GeneProduct:beta-ketoacyl reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:NADPH-dependent beta-ketoacyl reductase
Functional Category Confidence Code:5
COGs:pfam00106,COG1028
Secondary Functional Category(ies):fatty acid and phospholipid metabolism
EC Number:1.1.1.100
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAC46347.1| (AP005938) 2-deoxy-D-gluconate 3-dehydrogenase [Bradyrhizobium
           japonicum USDA 110]
          Length = 266

 Score =  221 bits (563), Expect = 3e-57
 Identities = 130/254 (51%), Positives = 164/254 (64%), Gaps = 10/254 (3%)

>dbj|BAB50065.1| (AP003001) short chain dehydrogenase/reductase family
           [Mesorhizobium loti]
          Length = 271

 Score =  221 bits (562), Expect = 4e-57
 Identities = 116/252 (46%), Positives = 156/252 (61%), Gaps = 3/252 (1%)








 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase.
CD-Length = 247 residues, 100.0% aligned
Score =  163 bits (413), Expect = 2e-41
Identities = 86/252 (34%), Positives = 124/252 (49%), Gaps = 14/252 (5%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  99.6% aligned
Score =  159 bits (402), Expect = 4e-40
Identities = 104/258 (40%), Positives = 140/258 (54%), Gaps = 16/258 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6335




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23477




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32176




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3166_cdd.html


    		




 
URL:

















    ReferenceID:34044




  
Author/Investigator(s):
        Campos-Garcia J, Caro AD, Najera R, Miller-Maier RM, Al-Tahhan RA, Soberon-Chavez G.





  
Title:
        The Pseudomonas aeruginosa rhlG gene encodes an NADPH-dependent beta-ketoacyl reductase which is specifically involved in rhamnolipid synthesis.




  
PubMed:
        9721281


MedLine:
        




    
Source:
J Bacteriol. 1998 Sep;180(17):4442-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9721281









           
Homologs By Global Alignment 

           

       



     
Gene ID:3166
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5093
                           PA3387_tr
                           translation of PA3387
                           256
                           99.21
                           99.60
                           0
                           1289.0
     

     
     
142844
                           gb|AAG06775.1|AE004760_3
                           (AE004760) beta-ketoacyl reductase [Pseudomonas aeruginosa PAO1]
                           256
                           99.21
                           99.60
                           0
                           1289.0
     

     
     
142845
                           gb|AAD53514.1|AF148964_1
                           (AF148964) NADPH-dependent beta-ketoacyl reductase [Pseudomonas aeruginosa]
                           256
                           99.21
                           99.60
                           0
                           1289.0
     

     
     
142846
                           gb|AAC62538.1|
                           (AF052586) beta-ketoacyl reductase [Pseudomonas aeruginosa]
                           344
                           52.61
                           59.59
                           88
                           869.0
     

     
     
142847
                           dbj|BAC46347.1|
                           (AP005938) 2-deoxy-D-gluconate 3-dehydrogenase [Bradyrhizobium japonicum USDA 110]
                           271
                           47.97
                           60.88
                           20
                           569.0
     

     






      

          
GC ORFID: 46168How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3387Glimmer Score: 

          
Start: 3206947Stop: 3207717

          
Length: 771


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3387 translation of PA3387Homolog Bit Score 501.0

          
Other Homologs:  t_PA3387 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCATCCCTATTTCAGCCTCGCCGGCCGCGTCGCCCTGGTCACCGGCGGCAGCCGCGGCATCGGCCAGATG 
ATCGCCCAGGGTCTTCTCGAAGCCGGCGCCCGCGTCTTCATCTGCGCACGTGACGCAGAGGCCTGCGCCGAC 
ACCGCCACACACCTCTCGGCCTACGGCGATTGCCAGGCGATCCCTGCCGACCTTTCCAGCGAAGCCGGTGCC 
CGGCGCCTGGCGCAGGCGCTCGGCGAACTCAGCGCGCGGCTGGATATCCTGGTGAACAACGCCGGCACCAGT 
TGGGGCGCAGCGCTGGAAAGCTATCCGGTGTCCGGCTGGGAAAAGGTCATGCAGCTCAATGTCACCTCGGTG 
TTTTCCTGCATCCAGCAACTGCTGCCGCTGCTGCGCCGCTCGGCCAGCGCGGAAAATCCGGCGCGAGTGATC 
AACATCGGCTCGGTGGCGGGCATCAGCGCCATGGGCGAACAAGCCTATGCCTACGGGCCGAGCAAGGCCGCG 
CTGCATCAACTGTCGCGGATGCTGGCGAAGGAACTGGTCGGCGAGCACATCAATGTCAACGTGATCGCTCCC 
GGACGCTTCCCGAGCCGCATGACCCGTCACATCGCCAATGACCCGCAGGCACTGGAGGCGGACAGCGCGTCG 
ATTCCCATGGGCCGCTGGGGTCGCCCGGAGGAGATGGCGGCGCTGGCGATCAGCCTGGCCGGCACCGCCGGC 
GCCTACATGACCGGCAACGTGATCCCCATCGACGGCGGTTTTCATCTCTGA