ViewGene
Locus Name:
GeneID:
Location:

GeneID:3163
TrivialName: PA14_67880
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67880
PAO1 Ortholog Locus:PA5140
Sequence Length:771
Protein Length:256
Start: 6061551
Stop: 6060781
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08525.1|AE004927_3 (AE004927) imidazoleglycerol-phosphate synthase, cyclase subunit [Pseudomonas aeruginosa PAO1]
Identities = 256/256 (100%), Positives = 256/256 (100%)
Sequence:
ATGGCACTGGCAAAACGCATCATCCCCTGCCTCGACGTGGACAATGGCCGAGTGGTCAAGGGCGTCAAGTTC
GAGAACATCCGCGACGCCGGCGATCCGGTCGAGATCGCCCGCCGCTACGATGAGCAGGGTGCCGACGAGATC
ACCTTCCTCGACATCACCGCCAGCGTCGACGGGCGCGACACCACCCTGCATACCGTCGAGCGCATGGCCAGT
CAGGTGTTCATTCCGCTGACCGTGGGCGGCGGCGTACGCAGCGTGCAGGACATCCGCAACCTGTTGAATGCC
GGCGCGGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCCGAGTTCGTCGGCGAGGCCGCCGACCGC
TTCGGCTCGCAGTGCATCGTGGTCGCCATCGATGCGAAGAAGGTTTCCGCCCCGGGCGAGGCGCCGCGCTGG
GAAATCTTCACCCATGGCGGGCGCAAGCCCACCGGGCTGGATGCCGTGCTCTGGGCGAAGAAGATGGAAGAC
CTGGGCGCCGGCGAGATTCTCCTGACCAGCATGGACCAGGACGGCGTGAAGAGCGGTTACGACCTGGGCGTG
ACCCGCGCCATCAGCGAGGCGGTGAACGTACCGGTGATTGCTTCCGGCGGCGTCGGCAACCTGGAGCACCTG
GCCGCCGGCATCCTCGAGGGCAAGGCCGACGCGGTGCTCGCGGCGAGCATCTTCCACTTCGGCGAGTACACC
GTGCCGGAAGCCAAGGCCTACCTCGCCAGCCGCGGTATCGTGGTGCGCTGA
Translation:
MALAKRIIPCLDVDNGRVVKGVKFENIRDAGDPVEIARRYDEQGADEITFLDITASVDGRDTTLHTVERMAS
QVFIPLTVGGGVRSVQDIRNLLNAGADKVSINTAAVFNPEFVGEAADRFGSQCIVVAIDAKKVSAPGEAPRW
EIFTHGGRKPTGLDAVLWAKKMEDLGAGEILLTSMDQDGVKSGYDLGVTRAISEAVNVPVIASGGVGNLEHL
AAGILEGKADAVLAASIFHFGEYTVPEAKAYLASRGIVVR*
AnnotationID:3171GeneID:3163
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 12:12:35
Gene Name:hisF1
Confidence Code:2
GeneProduct:imidazoleglycerol phosphate synthase, cyclase subunit
Cell Localization:()
Synonyms:hisF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:histidine biosynthesis protein
Functional Category Confidence Code:5
COGs:COG0107,pfam00977
Secondary Functional Category(ies):
EC Number:4.1.3.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58760.1| (AE016875) imidazoleglycerol phosphate synthase, cyclase subunit
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 256

 Score =  480 bits (1235), Expect = e-135
 Identities = 240/256 (93%), Positives = 251/256 (98%)

>gb|AAN65924.1|AE016220_6 (AE016775) imidazoleglycerol phosphate synthase, cyclase subunit
           [Pseudomonas putida KT2440]
          Length = 256

 Score =  476 bits (1226), Expect = e-134
 Identities = 240/256 (93%), Positives = 249/256 (97%)

>dbj|BAC65276.1| (AB091436) imidazole glycerol phosphate synthase [Burkholderia
           multivorans]
          Length = 257

 Score =  383 bits (983), Expect = e-106
 Identities = 190/256 (74%), Positives = 224/256 (87%)








 
Structural Features:
COG0107, HisF, Imidazoleglycerol-phosphate synthase [Amino acid transport and metabolism]
CD-Length = 256 residues,  98.8% aligned
Score =  411 bits (1059), Expect = 3e-116
Identities = 183/254 (72%), Positives = 213/254 (83%), Gaps = 1/254 (0%)

pfam00977, His_biosynth, Histidine biosynthesis protein.
CD-Length = 240 residues, 100.0% aligned
Score =  306 bits (786), Expect = 1e-84
Identities = 147/247 (59%), Positives = 185/247 (74%), Gaps = 10/247 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6329




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23471




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34948




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3163_cdd.html


    		




 
URL:

















    ReferenceID:35787




  
Author/Investigator(s):
        Erbeznik M, Strobel HJ, Dawson KA.





  
Title:
        Organization and sequence of histidine biosynthesis genes hisH, -A, -F, and -IE in Thermoanaerobacter ethanolicus.




  
PubMed:
        10594232


MedLine:
        




    
Source:
Curr Microbiol. 2000 Feb;40(2):140-2.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10594232

















    ReferenceID:35789




  
Author/Investigator(s):
        Burrows LL, Charter DF, Lam JS.





  
Title:
        Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.




  
PubMed:
        8939432


MedLine:
        




    
Source:
Mol Microbiol. 1996 Nov;22(3):481-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8939432









           
Homologs By Global Alignment 

           

       



     
Gene ID:3163
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5090
                           PA5140_tr
                           translation of PA5140
                           256
                           99.99
                           99.99
                           0
                           1293.0
     

     
     
142675
                           gb|AAG08525.1|AE004927_3
                           (AE004927) imidazoleglycerol-phosphate synthase, cyclase subunit [Pseudomonas aeruginosa PAO1]
                           256
                           99.99
                           99.99
                           0
                           1293.0
     

     
     
142676
                           gb|AAO58760.1|
                           (AE016875) imidazoleglycerol phosphate synthase, cyclase subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           256
                           93.75
                           98.04
                           0
                           1235.0
     

     
     
142677
                           gb|AAN65924.1|AE016220_6
                           (AE016775) imidazoleglycerol phosphate synthase, cyclase subunit [Pseudomonas putida KT2440]
                           256
                           93.75
                           97.26
                           0
                           1226.0
     

     
     
142679
                           emb|CAD16653.1|
                           (AL646072) PROBABLE CYCLASE (IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE-SUBUNIT WITH HISH) PROTEIN [Ralstonia solanacearum]
                           257
                           73.92
                           85.60
                           2
                           977.0
     

     






      

          
GC ORFID: 46165How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5140Glimmer Score: 

          
Start: 5325236Stop: 5324466

          
Length: 771


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5140 translation of PA5140Homolog Bit Score 502.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6488 (ORF: BLASTX 478.0), t_PA5140 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACTGGCAAAACGCATCATCCCCTGCCTCGACGTGGACAATGGCCGAGTGGTCAAGGGCGTCAAGTTC 
GAGAACATCCGCGACGCCGGCGATCCGGTCGAGATCGCCCGCCGCTACGATGAGCAGGGTGCCGACGAGATC 
ACCTTCCTCGACATCACCGCCAGCGTCGACGGGCGCGACACCACCCTGCATACCGTCGAGCGCATGGCCAGT 
CAGGTGTTCATTCCGCTGACCGTGGGCGGCGGCGTACGCAGCGTGCAGGACATCCGCAACCTGTTGAATGCC 
GGCGCGGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCCGAGTTCGTCGGCGAGGCCGCCGACCGC 
TTCGGCTCGCAGTGCATCGTGGTCGCCATCGATGCGAAGAAGGTTTCCGCCCCGGGCGAGGCGCCGCGCTGG 
GAAATCTTCACCCATGGCGGGCGCAAGCCCACCGGGCTGGATGCCGTGCTCTGGGCGAAGAAGATGGAAGAC 
CTGGGCGCCGGCGAGATTCTCCTGACCAGCATGGACCAGGACGGCGTGAAGAGCGGTTACGACCTGGGCGTG 
ACCCGCGCCATCAGCGAGGCGGTGAACGTACCGGTGATTGCTTCCGGCGGCGTCGGCAACCTGGAGCACCTG 
GCCGCCGGCATCCTCGAGGGCAAGGCCGACGCGGTGCTCGCGGCGAGCATCTTCCACTTCGGCGAGTACACC 
GTGCCGGAAGCCAAGGCCTACCTCGCCAGCCGCGGTATCGTGGTGCGCTGA