ViewGene
Locus Name:
GeneID:
Location:

GeneID:3160
TrivialName: PA14_25530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25530
PAO1 Ortholog Locus:PA2979
Sequence Length:765
Protein Length:254
Start: 2230872
Stop: 2231636
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06367.1|AE004723_11 (AE004723) 3-deoxy-manno-octulosonate cytidylyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 252/253 (99%), Positives = 252/253 (99%)
Sequence:
ATGACCCAGGCGTTCACTGTCGTCATTCCCGCCCGCTACGCCTCCACTCGTCTGCCCGGCAAGCCATTGCAG
GATATCGCCGGCCAGCCAATGATCCAGCGCGTCTGGAACCAGGCCCGCAAGAGCGCCGCCAGCCGCGTGGTC
GTCGCCACCGACGACGAACGCATCCTGGCGGCGTGCCAGGGGTTCGGCGCCGAGGCGCTGCTGACGCGTGCC
GAGCACAACTCCGGCACCGATCGCCTGGAGGAAGTCGCTTCCCGGCTCGGCCTGGCCAGCGATGCCATCGTG
GTCAACGTCCAGGGCGACGAACCGCTGATCCCCCCGGCGCTCATCGACCAGGTGGCGGCCAACCTGGCGGCG
CACCCGGAAGCCGCCATCGCTACCCTGGCCGAGCCGATCCATGAGGTCTCCGCGCTGTTCAATCCGAACGTG
GTCAAGGTCGCCACCGACATCGACGGCCTGGCCCTGACTTTCAGTCGTGCGCCCTTGCCCTGGGCTCGCGAC
GCCTTCGCCCGCGATCGCGACAGCCTGCCGGAGGGCGTGCCCTATCGTCGCCACATCGGCATCTACGCCTAT
CGCGTGGGTTTCCTTGCCGATTTCGTCGCCTGGGGACCGTGCTGGCTGGAGAACGCCGAAAGCCTCGAGCAA
TTGCGCGCGCTCTGGCACGGGGTGCGAATCCATGTGGCCGATGCCCGCGAAACCATGCTTCCGGGCGTGGAT
ACGCCGGAAGACCTCGAACGCGTTCGCCGCGTGCTGGGGGACTGA
Translation:
MTQAFTVVIPARYASTRLPGKPLQDIAGQPMIQRVWNQARKSAASRVVVATDDERILAACQGFGAEALLTRA
EHNSGTDRLEEVASRLGLASDAIVVNVQGDEPLIPPALIDQVAANLAAHPEAAIATLAEPIHEVSALFNPNV
VKVATDIDGLALTFSRAPLPWARDAFARDRDSLPEGVPYRRHIGIYAYRVGFLADFVAWGPCWLENAESLEQ
LRALWHGVRIHVADARETMLPGVDTPEDLERVRRVLGD*
AnnotationID:3168GeneID:3160
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 08:08:22
Gene Name:kdsB
Confidence Code:2
GeneProduct:3-deoxy-manno-octulosonate cytidylyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1212,pfam02348
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:2.7.7.38
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57310.1| (AE016869) 3-deoxy-D-manno-octulosonate cytidylyltransferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 254

 Score =  407 bits (1046), Expect = e-113
 Identities = 199/252 (78%), Positives = 223/252 (88%)

>gb|AAN67521.1|AE016380_9 (AE016781) 3-deoxy-D-manno-octulosonate cytidylyltransferase
           [Pseudomonas putida KT2440]
          Length = 254

 Score =  399 bits (1025), Expect = e-111
 Identities = 196/252 (77%), Positives = 215/252 (85%)

>gb|AAF95023.1| (AE004263) 3-deoxy-manno-octulosonate cytidylyltransferase [Vibrio
           cholerae]
          Length = 252

 Score =  278 bits (710), Expect = 3e-74
 Identities = 146/251 (58%), Positives = 178/251 (70%), Gaps = 3/251 (1%)






 
Structural Features:
COG1212, KdsB, CMP-2-keto-3-deoxyoctulosonic acid synthetase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 247 residues,  98.8% aligned
Score =  347 bits (892), Expect = 6e-97
Identities = 148/253 (58%), Positives = 169/253 (66%), Gaps = 9/253 (3%)

pfam02348, CTP_transf_3, Cytidylyltransferase. 
CD-Length = 213 residues,  99.5% aligned
Score =  204 bits (520), Expect = 1e-53
Identities = 92/221 (41%), Positives = 119/221 (53%), Gaps = 10/221 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6323




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23465




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36049




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3160_cdd.html


    		




 
URL:

















    ReferenceID:36450




  
Author/Investigator(s):
        Pazzani C, Rosenow C, Boulnois GJ, Bronner D, Jann K, Roberts IS.





  
Title:
        Molecular analysis of region 1 of the Escherichia coli K5 antigen gene cluster: a region encoding proteins involved in cell surface expression of capsular polysaccharide.




  
PubMed:
        8397187


MedLine:
        




    
Source:
J Bacteriol. 1993 Sep;175(18):5978-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8397187









           
Homologs By Global Alignment 

           

       



     
Gene ID:3160
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5085
                           PA2979_tr
                           translation of PA2979
                           254
                           99.21
                           99.21
                           0
                           1292.0
     

     
     
142607
                           gb|AAG06367.1|AE004723_11
                           (AE004723) 3-deoxy-manno-octulosonate cytidylyltransferase [Pseudomonas aeruginosa PAO1]
                           254
                           99.21
                           99.21
                           0
                           1292.0
     

     
     
142608
                           gb|AAO57310.1|
                           (AE016869) 3-deoxy-D-manno-octulosonate cytidylyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           254
                           78.34
                           87.79
                           0
                           1042.0
     

     
     
142609
                           gb|AAN67521.1|AE016380_9
                           (AE016781) 3-deoxy-D-manno-octulosonate cytidylyltransferase [Pseudomonas putida KT2440]
                           254
                           77.16
                           84.64
                           0
                           1021.0
     

     
     
142610
                           gb|AAQ61008.1|
                           (AE016922) 3-deoxy-manno-octulosonate cytidylyltransferase [Chromobacterium violaceum ATCC 12472]
                           254
                           61.81
                           72.04
                           3
                           748.0
     

     






      

          
GC ORFID: 46162How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2979Glimmer Score: 

          
Start: 2723053Stop: 2722289

          
Length: 765


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2979 translation of PA2979Homolog Bit Score 502.0

          
Other Homologs:  t_PA2979 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCAGGCGTTCACTGTCGTCATTCCCGCCCGCTACGCCTCCACTCGTCTGCCCGGCAAGCCATTGCAG 
GATATCGCCGGCCAGCCAATGATCCAGCGCGTCTGGAACCAGGCCCGCAAGAGCGCCGCCAGCCGCGTGGTC 
GTCGCCACCGACGACGAACGCATCCTGGCGGCGTGCCAGGGGTTCGGCGCCGAGGCGCTGCTGACGCGTGCC 
GAGCACAACTCCGGCACCGATCGCCTGGAGGAAGTCGCTTCCCGGCTCGGCCTGGCCAGCGATGCCATCGTG 
GTCAACGTCCAGGGCGACGAACCGCTGATCCCCCCGGCGCTCATCGACCAGGTGGCGGCCAACCTGGCGGCG 
CACCCGGAAGCCGCCATCGCTACCCTGGCCGAGCCGATCCATGAGGTCTCCGCGCTGTTCAATCCGAACGTG 
GTCAAGGTCGCCACCGACATCGACGGCCTGGCCCTGACTTTCAGTCGTGCGCCCTTGCCCTGGGCTCGCGAC 
GCCTTCGCCCGCGATCGCGACAGCCTGCCGGAGGGCGTGCCCTATCGTCGCCACATCGGCATCTACGCCTAT 
CGCGTGGGTTTCCTTGCCGATTTCGTCGCCTGGGGACCGTGCTGGCTGGAGAACGCCGAAAGCCTCGAGCAA 
TTGCGCGCGCTCTGGCACGGGGTGCGAATCCATGTGGCCGATGCCCGCGAAACCATGCTTCCGGGCGTGGAT 
ACGCCGGAAGACCTCGAACGCGTTCGCCGCGTGCTGGGGGACTGA