ViewGene

Locus Name:
GeneID:
Location:

GeneID: 3133

TrivialName: PA14_43440

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_43440

PAO1 Ortholog Locus: PA1629

Sequence Length: 786

Protein Length: 261

Start: 3870059

Stop: 3870844

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG05018.1|AE004590_9 (AE004590) probable enoyl-CoA hydratase/isomerase [Pseudomonas aeruginosa PAO1] Identities = 259/261 (99%), Positives = 259/261 (99%)

Sequence:

ATGTCCGCCGATCCTTCGCCCGTGGTTCTGCTCGAATTTCCCGCAGCCGATATCGCCCTGCTTCGCCTCAAT
CGTCCGCAAGCCAGGAACGCGCTGAACGACGAAGTGCGCCAGCGCCTCGCCAGCCACTTCCAGACGCTCGGC
GCGGATCCGGCGATCCGCGTCATCGTGCTCACCGGCGACTCGCGCTGCTTTGCAGCTGGCGCCGATCTCCGC
GATCTCTCGACGAGCACGGCGATCGGCCTGTACGGCCGCCACAGCGAGCGCTACTGGGAAGCCATCGCGCGC
TGCCCGAAGCCGGTGATCGCCGCGGTCAACGGCTTCGCCCTGGGCGGCGGCTGCGAACTGGCGATGCATTGC
GACCTGATCGTCGCCGGCGAGTCGGCACAGTTCGCCCAGCCGGAGATCAAGGTCGGGGTGATGCCGGGTGCC
GGCGGCACCCAGCGCCTGGTACGGGCGGTGGGCAAGTTCCAGGCGCTGCGCATGCTGTTCACCGGCTGCCTG
GTGAAGGCTCCGCAAGCGCTGGCCATGGGCCTGGTCAGCGAAGTGGTGGCGGACGAGTCGACGCTGGCGCGC
GCCCTGGAACTGGCCACGGAGATCGCCCGCCTGCCCCCTCTGGCGCTGGCGCAGATCAAGGAGGTGGTCCTG
GCCGGTGCCGACCTGCCGCTGGACAGTGCCCTGGCCCTGGAACGCAAGGCGTTCCAGTTGCTGTTCGACTCG
CAGGACCAGAAGGAAGGCATGCACGCCTTCCTGGAGAAACGCCCGCCGAACTACCAGGGGAAATAG

Translation:

MSADPSPVVLLEFPAADIALLRLNRPQARNALNDEVRQRLASHFQTLGADPAIRVIVLTGDSRCFAAGADLR
DLSTSTAIGLYGRHSERYWEAIARCPKPVIAAVNGFALGGGCELAMHCDLIVAGESAQFAQPEIKVGVMPGA
GGTQRLVRAVGKFQALRMLFTGCLVKAPQALAMGLVSEVVADESTLARALELATEIARLPPLALAQIKEVVL
AGADLPLDSALALERKAFQLLFDSQDQKEGMHAFLEKRPPNYQGK*

AnnotationID:3141 GeneID:3133

AnnotatorUID: diggins

Modification Date/Time: 2005-05-18 17:05:15

Gene Name:

Confidence Code: 3

GeneProduct: putative enoyl-CoA hydratase/isomerase

Cell Localization: ()

Synonyms: PA1629

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (13) Fatty acid and phospholipid metabolism

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: COG1024,pfam00378

Secondary Functional Category(ies):

EC Number: 4.2.1.17

Status: ACTIVE

Pathway:

Homology:

>gb|AAL54263.1| (AE009734) ENOYL-COA HYDRATASE [Brucella melitensis 16M]
          Length = 261

 Score =  339 bits (870), Expect = 8e-93
 Identities = 169/261 (64%), Positives = 207/261 (79%)

>gb|AAG10018.1|AF282240_2 (AF282240) enoyl CoA dehydratase [Acinetobacter sp. SE19]
          Length = 258

 Score =  326 bits (835), Expect = 9e-89
 Identities = 154/254 (60%), Positives = 207/254 (81%)

>gb|AAN68891.1|AE016520_11 (AE016786) enoyl-CoA hydratase/isomerase [Pseudomonas putida
           KT2440]
          Length = 257

 Score =  221 bits (563), Expect = 3e-57
 Identities = 115/251 (45%), Positives = 162/251 (64%)

Structural Features:

COG1024, CaiD, Enoyl-CoA hydratase/carnithine racemase [Lipid metabolism]
CD-Length = 257 residues,  96.9% aligned
Score =  222 bits (566), Expect = 4e-59
Identities = 119/250 (47%), Positives = 157/250 (62%), Gaps = 5/250 (2%)

pfam00378, ECH, Enoyl-CoA hydratase/isomerase family. 
CD-Length = 168 residues, 100.0% aligned
Score =  168 bits (427), Expect = 6e-43
Identities = 78/168 (46%), Positives = 113/168 (67%), Gaps = 1/168 (0%)

Genomic Context:

Comment:

ReferenceID:6269

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:23411

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:37250

Author/Investigator(s):

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: Misc (text/plain)

Data: 3133_cdd.html

URL:

Homologs By Global Alignment

Gene ID:3133

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

5032 PA1629_tr translation of PA1629 261 99.23 99.23 0 1301.0

141524 gb|AAG05018.1|AE004590_9 (AE004590) probable enoyl-CoA hydratase/isomerase [Pseudomonas aeruginosa PAO1] 261 99.23 99.23 0 1301.0

5033 PA3591_tr translation of PA3591 265 70.18 78.49 4 893.0

141525 gb|AAG06979.1|AE004779_11 (AE004779) probable enoyl-CoA hydratase/isomerase [Pseudomonas aeruginosa PAO1] 265 70.18 78.49 4 893.0

141528 emb|CAC28159.1| (AJ278289) putative hydrolase [Thauera aromatica] 261 65.13 76.24 5 855.0

GC ORFID: `46135`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA1629`	Glimmer Score:
Start: `1083850`	Stop: `1083065`
Length: `786`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TAG`	Truncated Stop:
Homolog: `t_PA1629 translation of PA1629`	Homolog Bit Score `505.0`
Other Homologs: `t_PA3591 (ORF: BLASTX 347.0), psyr_15may02_Scaffold4_revised_gene5257 (ORF: BLASTX 198.0), t_PA1629 (ORF: GLIMMER)`
GC ORF Sequence
ATGTCCGCCGATCCTTCGCCCGTGGTTCTGCTCGAATTTCCCGCAGCCGATATCGCCCTGCTTCGCCTCAAT CGTCCGCAAGCCAGGAACGCGCTGAACGACGAAGTGCGCCAGCGCCTCGCCAGCCACTTCCAGACGCTCGGC GCGGATCCGGCGATCCGCGTCATCGTGCTCACCGGCGACTCGCGCTGCTTTGCAGCTGGCGCCGATCTCCGC GATCTCTCGACGAGCACGGCGATCGGCCTGTACGGCCGCCACAGCGAGCGCTACTGGGAAGCCATCGCGCGC TGCCCGAAGCCGGTGATCGCCGCGGTCAACGGCTTCGCCCTGGGCGGCGGCTGCGAACTGGCGATGCATTGC GACCTGATCGTCGCCGGCGAGTCGGCACAGTTCGCCCAGCCGGAGATCAAGGTCGGGGTGATGCCGGGTGCC GGCGGCACCCAGCGCCTGGTACGGGCGGTGGGCAAGTTCCAGGCGCTGCGCATGCTGTTCACCGGCTGCCTG GTGAAGGCTCCGCAAGCGCTGGCCATGGGCCTGGTCAGCGAAGTGGTGGCGGACGAGTCGACGCTGGCGCGC GCCCTGGAACTGGCCACGGAGATCGCCCGCCTGCCCCCTCTGGCGCTGGCGCAGATCAAGGAGGTGGTCCTG GCCGGTGCCGACCTGCCGCTGGACAGTGCCCTGGCCCTGGAACGCAAGGCGTTCCAGTTGCTGTTCGACTCG CAGGACCAGAAGGAAGGCATGCACGCCTTCCTGGAGAAACGCCCGCCGAACTACCAGGGGAAATAG