ViewGene

Locus Name:
GeneID:
Location:

GeneID: 3132

TrivialName: PA14_50550

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_50550

PAO1 Ortholog Locus: PA1071

Sequence Length: 768

Protein Length: 255

Start: 4490502

Stop: 4491269

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG04460.1|AE004539_2 (AE004539) branched-chain amino acid transport protein BraF [Pseudomonas aeruginosa PAO1] Identities = 255/255 (100%), Positives = 255/255 (100%)

Sequence:

ATGAGCCGACCGATTCTCGAAGTGAGCGGCCTGACCATGCGCTTCGGCGGCCTGCTGGCCGTCAACGGCGTC
AACCTGAAGGTCGAGGAAAAGCAGGTGGTCTCGATGATCGGCCCGAACGGCGCCGGCAAGACCACCGTGTTC
AACTGCCTGACCGGCTTCTACCAGCCCACCGGCGGCCTGATCCGCCTCGACGGCGAGGAGATCCAGGGCCTG
CCCGGCCACAAGATCGCTCGCAAGGGCGTGGTGCGGACCTTCCAGAACGTCCGCCTGTTCAAGGAAATGACC
GCGGTGGAGAACCTGCTGGTCGCCCAGCACCGCCACCTCAACACCAACTTCCTCGCCGGCCTGTTCAAGACC
CCGGCATTCCGCCGCAGCGAGCGCGAGGCCATGGAGTACGCCGCGCACTGGCTGGAGGAAGTCAACCTTACC
GAGTTCGCCAACCGCAGCGCCGGTACCCTCGCCTACGGCCAGCAGCGACGCCTGGAGATCGCCCGCTGCATG
ATGACCCGCCCGCGGATCCTCATGCTCGACGAGCCGGCCGCCGGCCTCAACCCGAAGGAGACCGACGACCTC
AAGGCGCTGATCGCCAAGCTGCGCAGCGAGCACAACGTGACGGTATTGCTGATCGAGCACGACATGAAGCTG
GTGATGAGCATTTCCGACCATATCGTGGTGATCAACCAGGGCGCCCCCCTGGCCGACGGGACGCCGGAGCAG
ATCCGCGACAACCCGGACGTGATCAAGGCTTATCTGGGGGAAGCGTGA

Translation:

MSRPILEVSGLTMRFGGLLAVNGVNLKVEEKQVVSMIGPNGAGKTTVFNCLTGFYQPTGGLIRLDGEEIQGL
PGHKIARKGVVRTFQNVRLFKEMTAVENLLVAQHRHLNTNFLAGLFKTPAFRRSEREAMEYAAHWLEEVNLT
EFANRSAGTLAYGQQRRLEIARCMMTRPRILMLDEPAAGLNPKETDDLKALIAKLRSEHNVTVLLIEHDMKL
VMSISDHIVVINQGAPLADGTPEQIRDNPDVIKAYLGEA*

AnnotationID:3140 GeneID:3132

AnnotatorUID: danlee

Modification Date/Time: 2004-05-19 17:05:15

Gene Name: braF

Confidence Code: 1

GeneProduct: branched-chain amino acid transport protein BraF

Cell Localization: (5) Inner membrane-associated

Synonyms: PA1071

Cell Localization Confidence Code: 1

MolecularFunction:

Functional Category: (27) Transport of small molecules

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: COG0411

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Homology:

Structural Features:

COG0411, LivG, ABC-type branched-chain amino acid transport systems, ATPase 
component. Score: 352, 3e-98
COG4674, Uncharacterized ABC-type transport system, ATPase 
component. Score: 200, 1e-52
COG0410, LivF, ABC-type branched-chain amino acid transp

Genomic Context:

Adjacent to genes BraC, BraD, BraE, and BraG, reported to constitute a
transport system for branched-chain amino acids�

Comment:

ReferenceID:6267

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:23409

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:32219

Author/Investigator(s): Hoshino T, Kose K.

Title: Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.

PubMed: 91008919

MedLine:

Source:

J Bacteriol. 1990 Oct;172(10):5531-9

Reference Type: Journal

Data:

URL:

Homologs By Global Alignment

Gene ID:3132

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

5030 PA1071_tr translation of PA1071 255 99.99 99.99 0 1300.0

141402 gb|AAG04460.1|AE004539_2 (AE004539) branched-chain amino acid transport protein BraF [Pseudomonas aeruginosa PAO1] 255 99.99 99.99 0 1300.0

141403 dbj|BAA14257.1| (D90223) peripheral membrane protein [Pseudomonas aeruginosa] 255 99.99 99.99 0 1300.0

141404 gb|AAR19312.1| (AY426537) putative branched-chain amino acid transporter ATP-binding protein [symbiont bacterium of Paederus fuscipes] 255 91.37 95.68 0 1198.0

141405 gb|AAO57567.1| (AE016870) high-affinity branched-chain amino acid ABC transporter, ATP-binding protein [Pseudomonas syringae pv. tomato str. DC3000] 255 87.84 92.94 0 1152.0

GC ORFID: `46134`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA1071`	Glimmer Score:
Start: `463406`	Stop: `462639`
Length: `768`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA1071 translation of PA1071`	Homolog Bit Score `505.0`
Other Homologs: `psyr_15may02_Scaffold5_revised_gene6076 (ORF: BLASTX 450.0), t_PA4910 (ORF: BLASTX 285.0), psyr_15may02_Scaffold8_revised_gene7645 (ORF: BLASTX 274.0), t_PA1071 (ORF: GLIMMER)`
GC ORF Sequence
ATGAGCCGACCGATTCTCGAAGTGAGCGGCCTGACCATGCGCTTCGGCGGCCTGCTGGCCGTCAACGGCGTC AACCTGAAGGTCGAGGAAAAGCAGGTGGTCTCGATGATCGGCCCGAACGGCGCCGGCAAGACCACCGTGTTC AACTGCCTGACCGGCTTCTACCAGCCCACCGGCGGCCTGATCCGCCTCGACGGCGAGGAGATCCAGGGCCTG CCCGGCCACAAGATCGCTCGCAAGGGCGTGGTGCGGACCTTCCAGAACGTCCGCCTGTTCAAGGAAATGACC GCGGTGGAGAACCTGCTGGTCGCCCAGCACCGCCACCTCAACACCAACTTCCTCGCCGGCCTGTTCAAGACC CCGGCATTCCGCCGCAGCGAGCGCGAGGCCATGGAGTACGCCGCGCACTGGCTGGAGGAAGTCAACCTTACC GAGTTCGCCAACCGCAGCGCCGGTACCCTCGCCTACGGCCAGCAGCGACGCCTGGAGATCGCCCGCTGCATG ATGACCCGCCCGCGGATCCTCATGCTCGACGAGCCGGCCGCCGGCCTCAACCCGAAGGAGACCGACGACCTC AAGGCGCTGATCGCCAAGCTGCGCAGCGAGCACAACGTGACGGTATTGCTGATCGAGCACGACATGAAGCTG GTGATGAGCATTTCCGACCATATCGTGGTGATCAACCAGGGCGCCCCCCTGGCCGACGGGACGCCGGAGCAG ATCCGCGACAACCCGGACGTGATCAAGGCTTATCTGGGGGAAGCGTGA