ViewGene
Locus Name:
GeneID:
Location:

GeneID:3123
TrivialName: PA14_15990
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_15990
PAO1 Ortholog Locus:PA3743
Sequence Length:759
Protein Length:252
Start: 1361846
Stop: 1362604
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07130.1|AE004793_7 (AE004793) tRNA (guanine-N1)-methyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 251/252 (99%), Positives = 251/252 (99%)
Sequence:
ATGGACAAGCGTTTGTGGGTGGGCGTCGTCAGCATCTTTCCGGAGATGTTCCGCGCGATCAGTGACTATGGC
ATCACCAGCCGTGCGGTAAAGCAGGGGTTGCTCACGCTGACCTGCTGGAATCCACGGGTCTACACCGAGGAC
CGCCACCAGACGGTGGACGACCGGCCCTTCGGCGGTGGCCCTGGCATGGTGATGAAGATCAAGCCTCTGGAG
GGCGCGCTGGCGGATGCCAGGCAAGCCGCCGGGGGCAGGAAGGCGAAGGTGATCTACCTTTCGCCGCAAGGC
CGCCAGCTGACGCAGGCGGGCGTACGCGAACTGGCCGAAGAGGAAGCGTTGATCCTCATCGCCGGGCGCTAC
GAAGGCATCGACGAGCGCTTCATCGAAGAGCACGTCGATGAAGAATGGTCGATCGGCGATTATGTCCTGTCC
GGCGGTGAGTTGCCGGCCATGGTGCTGGTCGATGCAGTGACGCGGTTGCTGCCCGGTGCATTGGGTCATGCA
GATTCCGCCGAGGAGGACTCCTTCACGGATGGCCTGCTCGACTGCCCGCACTACACCCGACCCGAGGTGTAT
GCGGACAAACGTGTTCCTGAAGTGTTGCTCAGTGGCAACCATGAACACATCCGGCGTTGGCGTTTGCAGCAG
GCCCTTGGGCGGACCTGGGAACGACGCGCCGATCTTCTGGATAGCCGCTCGCTTTCTGGAGAAGAGCAGAAG
CTGCTGGCGGAATACATCCGTCAGCGGGACGATAGTTAA
Translation:
MDKRLWVGVVSIFPEMFRAISDYGITSRAVKQGLLTLTCWNPRVYTEDRHQTVDDRPFGGGPGMVMKIKPLE
GALADARQAAGGRKAKVIYLSPQGRQLTQAGVRELAEEEALILIAGRYEGIDERFIEEHVDEEWSIGDYVLS
GGELPAMVLVDAVTRLLPGALGHADSAEEDSFTDGLLDCPHYTRPEVYADKRVPEVLLSGNHEHIRRWRLQQ
ALGRTWERRADLLDSRSLSGEEQKLLAEYIRQRDDS*
AnnotationID:3131GeneID:3123
AnnotatorUID: diggins
Modification Date/Time: 2005-04-22 09:09:58
Gene Name:trmD
Confidence Code:2
GeneProduct:tRNA (guanine-N1)-methyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(24) Transcription, RNA processing and degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0336,pfam01746
Secondary Functional Category(ies):
EC Number:2.1.1.31
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67086.1|AE016337_9 (AE016779) tRNA (guanine-N1)-methyltransferase [Pseudomonas putida
           KT2440]
          Length = 250

 Score =  421 bits (1082), Expect = e-117
 Identities = 206/248 (83%), Positives = 227/248 (91%), Gaps = 1/248 (0%)

>gb|AAN81578.1|AE016764_260 (AE016764) tRNA (Guanine-N(1)-)-methyltransferase [Escherichia coli
           CFT073]
          Length = 255

 Score =  340 bits (873), Expect = 3e-93
 Identities = 159/241 (65%), Positives = 197/241 (81%), Gaps = 1/241 (0%)






 
Structural Features:
COG0336, TrmD, tRNA-(guanine-N1)-methyltransferase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 240 residues,  99.6% aligned
Score =  359 bits (922), Expect = 5e-100
Identities = 143/242 (59%), Positives = 179/242 (73%), Gaps = 4/242 (1%)

pfam01746, tRNA_m1G_MT, tRNA (Guanine-1)-methyltransferase.
CD-Length = 208 residues,  95.7% aligned
Score =  269 bits (690), Expect = 4e-73
Identities = 113/202 (55%), Positives = 149/202 (73%), Gaps = 3/202 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6249




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23391




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31815




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3123_cdd.html


    		




 
URL:

















    ReferenceID:34263




  
Author/Investigator(s):
        Jin S, Benedik MJ.





  
Title:
        Sequences of the Serratia marcescens rplS and trmD genes.




  
PubMed:
        8045416


MedLine:
        




    
Source:
Gene. 1994 Jul 22;145(1):147-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8045416

















    ReferenceID:34264




  
Author/Investigator(s):
        Bystrom AS, Hjalmarsson KJ, Wikstrom PM, Bjork GR.





  
Title:
        The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide.




  
PubMed:
        6357787


MedLine:
        




    
Source:
EMBO J. 1983;2(6):899-905.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6357787









           
Homologs By Global Alignment 

           

       



     
Gene ID:3123
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5020
                           PA3743_tr
                           translation of PA3743
                           252
                           99.60
                           99.60
                           0
                           1305.0
     

     
     
141090
                           gb|AAG07130.1|AE004793_7
                           (AE004793) tRNA (guanine-N1)-methyltransferase [Pseudomonas aeruginosa PAO1]
                           252
                           99.60
                           99.60
                           0
                           1305.0
     

     
     
141091
                           gb|AAO54996.1|
                           (AE016861) tRNA (guanine-N1)-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           252
                           85.71
                           91.26
                           2
                           1118.0
     

     
     
141092
                           gb|AAN67086.1|AE016337_9
                           (AE016779) tRNA (guanine-N1)-methyltransferase [Pseudomonas putida KT2440]
                           252
                           81.74
                           90.07
                           2
                           1079.0
     

     
     
141094
                           emb|CAC92525.1|
                           (AJ414156) tRNA (guanine-N1)-methyltransferase [Yersinia pestis CO92]
                           252
                           64.28
                           78.96
                           6
                           882.0
     

     






      

          
GC ORFID: 46125How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3743Glimmer Score: 

          
Start: 3592091Stop: 3591333

          
Length: 759


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3743 translation of PA3743Homolog Bit Score 507.0

          
Other Homologs:  t_PA3743 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGACAAGCGTTTGTGGGTGGGCGTCGTCAGCATCTTTCCGGAGATGTTCCGCGCGATCAGTGACTATGGC 
ATCACCAGCCGTGCGGTAAAGCAGGGGTTGCTCACGCTGACCTGCTGGAATCCACGGGTCTACACCGAGGAC 
CGCCACCAGACGGTGGACGACCGGCCCTTCGGCGGTGGCCCTGGCATGGTGATGAAGATCAAGCCTCTGGAG 
GGCGCGCTGGCGGATGCCAGGCAAGCCGCCGGGGGCAGGAAGGCGAAGGTGATCTACCTTTCGCCGCAAGGC 
CGCCAGCTGACGCAGGCGGGCGTACGCGAACTGGCCGAAGAGGAAGCGTTGATCCTCATCGCCGGGCGCTAC 
GAAGGCATCGACGAGCGCTTCATCGAAGAGCACGTCGATGAAGAATGGTCGATCGGCGATTATGTCCTGTCC 
GGCGGTGAGTTGCCGGCCATGGTGCTGGTCGATGCAGTGACGCGGTTGCTGCCCGGTGCATTGGGTCATGCA 
GATTCCGCCGAGGAGGACTCCTTCACGGATGGCCTGCTCGACTGCCCGCACTACACCCGACCCGAGGTGTAT 
GCGGACAAACGTGTTCCTGAAGTGTTGCTCAGTGGCAACCATGAACACATCCGGCGTTGGCGTTTGCAGCAG 
GCCCTTGGGCGGACCTGGGAACGACGCGCCGATCTTCTGGATAGCCGCTCGCTTTCTGGAGAAGAGCAGAAG 
CTGCTGGCGGAATACATCCGTCAGCGGGACGATAGTTAA