ViewGene
Locus Name:
GeneID:
Location:

GeneID:312
TrivialName: PA14_47190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47190
PAO1 Ortholog Locus:PA1318
Sequence Length:1977
Protein Length:658
Start: 4205551
Stop: 4203575
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04707.1|AE004561_3 (AE004561) cytochrome o ubiquinol oxidase subunit I [Pseudomonas aeruginosa PAO1]
Identities = 657/658 (99%), Positives = 657/658 (99%)
Sequence:
ATGTTCGGGAAACTGACACTCTCGGCCGTGCCTTACCACGAGCCCATCGTCATGGTCACACTGGCCGTCGTC
GCCCTGCTGGGCCTCGGCGTGGTCGGTGCGATCACCTACTACCGCAAGTGGACCTACCTGTGGACCGAGTGG
CTGACCTCGGTCGACCACAAGAAGATCGGCGTGATGTACATCGTCGTCGCCCTGGTCATGCTGGTCCGCGGC
TTCGCCGACGCGATCATGATGCGCGGCCAGCTGGCCCTGGCCGAAGGCGCCAACCACGGCTACCTGCCGCCG
GAGCACTACGACCAGATCTTCACCGCGCATGGCGTGATCATGATCATCTTCATGGCCATGCCGTTCATGACC
GGCCTGATGAACCTGGCCGTGCCGCTGCAGATCGGCGCGCGCGACGTGGCGTTCCCCTTCCTCAACTCGCTG
AGCTTCTGGCTGCTCGTGGTCAGCGCCATGCTGGTCAACGTGTCGCTGGGCCTGGGCGAATTCGCCCGTACC
GGCTGGGTGGCCTATCCGCCGCTGTCGGAGCTGGCCTATAGCCCCGGGGTAGGGGTGGATTACTACATCTGG
GCGCTACAGATATCCGGGATGGGCACGTTGCTCACGGGGATCAACTTCCTGGTCACCGTGTTCAAGATGCGT
GCCCCGGGCATGAAGCTGATGCAGATGCCGATCTTCACCTGGACCTGCACCTTCGCCAACATCCTGATCGTC
GCCTCGTTCCCGATCCTCACCGCTGCGCTGGGCCTGCTGTCGCTCGACCGCTACCTGGACATGCACTTCTTC
ACCAACGAGCTCGGCGGCAACGCCATGATGTACATCAACCTCTTCTGGGCCTGGGGCCATCCCGAGGTGTAC
ATCCTGATCCTGCCGGCCTTCGGTATCTTCTCCGAGGTCACCGCGACCTTCGCCGGCAAGCGCATGTTCGGC
TACAAGTCGATGGTCTGGGCCAGCGCCGCGATCACCTTCCTCGGCTTCACCGTGTGGCTGCACCACTTCTTC
ACCATGGGTTCGGGCGGTGACGTCAACGGCTTCTTCGGCGTGGCCACCATGCTGATCTCGATCCCCACCGGG
GTGAAGCTGTTCAACTGGCTGTTCACCATCTACAAGGGCCGCCTGCGCTTCAGCACGCCGATCCTGTGGACC
CTGGGCTTCATGGTGACCTTCACCATCGGCGGCATGACCGGCGTCCTGCTGGCCATCCCGGGCGCGGACTTC
CTGCTGCACAACAGCCTGTTCCTGATCGCCCACTTCCACAACACCATCATCGGCGGCGCGGTATTCGGCTAC
CTGGCCGGCTTCGCCTTCTGGTTCCCGAAAGCGTTCGGCTTCACCCTCGACGAGAAGTGGGGCAAGCGCTCC
TTCTGGTGCTGGCTGGTGGGCTTCTACATGGCCTTCATGCCGCTCTACATCCTCGGCTTCATGGGCATGACC
CGTCGCCTGAACCACTACGACAACCCGTTGTGGAAGCCCTACCTGGTGGTCGCGTTCTTCGGCGCCGTGCTG
ATCTTCTGCGGCATTGCCTGCCAGCTGATCCAGCTGTTCGTCTCGGTCCGCAACCGCAAGCAACTGGCCGAC
GTCAATGGCGACCCGTGGGAAGGCCGCACCCTGGAATGGGCCACTTCCTCGCCGCCGCCGTTCTACAACTTC
GCCGAGCTGCCGAAGGTACAGGACGTGGATGCCTTCCACGACATGAAGAAGGCCGGCACCGCCTACCGCAAG
CTGCCGGCCTACCAGCCGATCCACATGCCGAAGAACACCGCCGCGGGCTTCTCCATCGCCGTGTTCGCCTTC
GTCTTCGGCTTCGCCGCCATCTGGCATATCTGGTGGCTCATGGCGGTCGGCTTCGTCGGCATGATCGGCTCC
TTCATCGTGCGCAGCTACAACCAGGACGTCGACTACTACGTCCAGCCCGAAGAGATCGAGAAGATCGAAAGC
GCCCGTTTCCAGCAACTCGCCAAGCAGGTCTGA
Translation:
MFGKLTLSAVPYHEPIVMVTLAVVALLGLGVVGAITYYRKWTYLWTEWLTSVDHKKIGVMYIVVALVMLVRG
FADAIMMRGQLALAEGANHGYLPPEHYDQIFTAHGVIMIIFMAMPFMTGLMNLAVPLQIGARDVAFPFLNSL
SFWLLVVSAMLVNVSLGLGEFARTGWVAYPPLSELAYSPGVGVDYYIWALQISGMGTLLTGINFLVTVFKMR
APGMKLMQMPIFTWTCTFANILIVASFPILTAALGLLSLDRYLDMHFFTNELGGNAMMYINLFWAWGHPEVY
ILILPAFGIFSEVTATFAGKRMFGYKSMVWASAAITFLGFTVWLHHFFTMGSGGDVNGFFGVATMLISIPTG
VKLFNWLFTIYKGRLRFSTPILWTLGFMVTFTIGGMTGVLLAIPGADFLLHNSLFLIAHFHNTIIGGAVFGY
LAGFAFWFPKAFGFTLDEKWGKRSFWCWLVGFYMAFMPLYILGFMGMTRRLNHYDNPLWKPYLVVAFFGAVL
IFCGIACQLIQLFVSVRNRKQLADVNGDPWEGRTLEWATSSPPPFYNFAELPKVQDVDAFHDMKKAGTAYRK
LPAYQPIHMPKNTAAGFSIAVFAFVFGFAAIWHIWWLMAVGFVGMIGSFIVRSYNQDVDYYVQPEEIEKIES
ARFQQLAKQV*
AnnotationID:322GeneID:312
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 09:09:57
Gene Name:cyoB
Confidence Code:2
GeneProduct:cytochrome o ubiquinol oxidase subunit I
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:ubiquinol oxidase polypeptide I
Functional Category Confidence Code:5
COGs:COG0843,pfam00115
Secondary Functional Category(ies):
EC Number:1.10.3.-
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA76357.1| (AB016787) cytochrome o ubiquinol oxidase B [Pseudomonas putida]
          Length = 672

 Score = 1068 bits (2761), Expect = 0.0
 Identities = 497/656 (75%), Positives = 568/656 (86%)

>emb|CAD16623.1| (AL646072) PROBABLE TRANSMEMBRANE CYTOCHROME O UBIQUINOL OXIDASE
           (SUBUNIT I) OXIDOREDUCTASE PROTEIN [Ralstonia
           solanacearum]
          Length = 659

 Score = 1047 bits (2707), Expect = 0.0
 Identities = 481/658 (73%), Positives = 559/658 (84%), Gaps = 1/658 (0%)

>gb|AAN79020.1|AE016756_203 (AE016756) Ubiquinol oxidase polypeptide I [Escherichia coli
           CFT073]
          Length = 663

 Score =  980 bits (2533), Expect = 0.0
 Identities = 448/656 (68%), Positives = 544/656 (82%)






 
Structural Features:
COG0843, CyoB, Heme/copper-type cytochrome/quinol oxidases, subunit 1 
[Energy production and conversion]
CD-Length = 566 residues, 100.0% aligned
Score =  611 bits (1576), Expect = 1e-175
Identities = 304/574 (52%), Positives = 388/574 (67%), Gaps = 11/574 (1%)

pfam00115, COX1, Cytochrome C and Quinol oxidase polypeptide I.
CD-Length = 458 residues,  99.8% aligned
Score =  497 bits (1280), Expect = 2e-141
Identities = 224/459 (48%), Positives = 305/459 (66%), Gaps = 3/459 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:627




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17769




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37461




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:312_cdd.html


    		




 
URL:

















    ReferenceID:38016




  
Author/Investigator(s):
        Chepuri V, Lemieux L, Au DC, Gennis RB.





  
Title:
        The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases.




  
PubMed:
        2162835


MedLine:
        




    
Source:
J Biol Chem. 1990 Jul 5;265(19):11185-92






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2162835









           
Homologs By Global Alignment 

           

       



     
Gene ID:312
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
511
                           PA1318_tr
                           translation of PA1318
                           658
                           99.84
                           99.84
                           0
                           3507.0
     

     
     
25594
                           gb|AAG04707.1|AE004561_3
                           (AE004561) cytochrome o ubiquinol oxidase subunit I [Pseudomonas aeruginosa PAO1]
                           658
                           99.84
                           99.84
                           0
                           3507.0
     

     
     
25595
                           dbj|BAA76357.1|
                           (AB016787) cytochrome o ubiquinol oxidase B [Pseudomonas putida]
                           672
                           73.95
                           84.52
                           14
                           2757.0
     

     
     
25596
                           gb|AAN66438.1|AE016273_7
                           (AE016777) cytochrome o ubiquinol oxidase, subunit I [Pseudomonas putida KT2440]
                           672
                           73.66
                           84.07
                           14
                           2740.0
     

     
     
25597
                           dbj|BAB83594.1|
                           (AB066295) cytochrome o oxidase B [Pseudomonas putida]
                           672
                           73.66
                           84.07
                           14
                           2740.0
     

     






      

          
GC ORFID: 43314How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1318Glimmer Score: 

          
Start: 748358Stop: 750334

          
Length: 1977


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1318 translation of PA1318Homolog Bit Score 1355.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4441 (ORF: BLASTX 1044.0), t_PA1318 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTCGGGAAACTGACACTCTCGGCCGTGCCTTACCACGAGCCCATCGTCATGGTCACACTGGCCGTCGTC 
GCCCTGCTGGGCCTCGGCGTGGTCGGTGCGATCACCTACTACCGCAAGTGGACCTACCTGTGGACCGAGTGG 
CTGACCTCGGTCGACCACAAGAAGATCGGCGTGATGTACATCGTCGTCGCCCTGGTCATGCTGGTCCGCGGC 
TTCGCCGACGCGATCATGATGCGCGGCCAGCTGGCCCTGGCCGAAGGCGCCAACCACGGCTACCTGCCGCCG 
GAGCACTACGACCAGATCTTCACCGCGCATGGCGTGATCATGATCATCTTCATGGCCATGCCGTTCATGACC 
GGCCTGATGAACCTGGCCGTGCCGCTGCAGATCGGCGCGCGCGACGTGGCGTTCCCCTTCCTCAACTCGCTG 
AGCTTCTGGCTGCTCGTGGTCAGCGCCATGCTGGTCAACGTGTCGCTGGGCCTGGGCGAATTCGCCCGTACC 
GGCTGGGTGGCCTATCCGCCGCTGTCGGAGCTGGCCTATAGCCCCGGGGTAGGGGTGGATTACTACATCTGG 
GCGCTACAGATATCCGGGATGGGCACGTTGCTCACGGGGATCAACTTCCTGGTCACCGTGTTCAAGATGCGT 
GCCCCGGGCATGAAGCTGATGCAGATGCCGATCTTCACCTGGACCTGCACCTTCGCCAACATCCTGATCGTC 
GCCTCGTTCCCGATCCTCACCGCTGCGCTGGGCCTGCTGTCGCTCGACCGCTACCTGGACATGCACTTCTTC 
ACCAACGAGCTCGGCGGCAACGCCATGATGTACATCAACCTCTTCTGGGCCTGGGGCCATCCCGAGGTGTAC 
ATCCTGATCCTGCCGGCCTTCGGTATCTTCTCCGAGGTCACCGCGACCTTCGCCGGCAAGCGCATGTTCGGC 
TACAAGTCGATGGTCTGGGCCAGCGCCGCGATCACCTTCCTCGGCTTCACCGTGTGGCTGCACCACTTCTTC 
ACCATGGGTTCGGGCGGTGACGTCAACGGCTTCTTCGGCGTGGCCACCATGCTGATCTCGATCCCCACCGGG 
GTGAAGCTGTTCAACTGGCTGTTCACCATCTACAAGGGCCGCCTGCGCTTCAGCACGCCGATCCTGTGGACC 
CTGGGCTTCATGGTGACCTTCACCATCGGCGGCATGACCGGCGTCCTGCTGGCCATCCCGGGCGCGGACTTC 
CTGCTGCACAACAGCCTGTTCCTGATCGCCCACTTCCACAACACCATCATCGGCGGCGCGGTATTCGGCTAC 
CTGGCCGGCTTCGCCTTCTGGTTCCCGAAAGCGTTCGGCTTCACCCTCGACGAGAAGTGGGGCAAGCGCTCC 
TTCTGGTGCTGGCTGGTGGGCTTCTACATGGCCTTCATGCCGCTCTACATCCTCGGCTTCATGGGCATGACC 
CGTCGCCTGAACCACTACGACAACCCGTTGTGGAAGCCCTACCTGGTGGTCGCGTTCTTCGGCGCCGTGCTG 
ATCTTCTGCGGCATTGCCTGCCAGCTGATCCAGCTGTTCGTCTCGGTCCGCAACCGCAAGCAACTGGCCGAC 
GTCAATGGCGACCCGTGGGAAGGCCGCACCCTGGAATGGGCCACTTCCTCGCCGCCGCCGTTCTACAACTTC 
GCCGAGCTGCCGAAGGTACAGGACGTGGATGCCTTCCACGACATGAAGAAGGCCGGCACCGCCTACCGCAAG 
CTGCCGGCCTACCAGCCGATCCACATGCCGAAGAACACCGCCGCGGGCTTCTCCATCGCCGTGTTCGCCTTC 
GTCTTCGGCTTCGCCGCCATCTGGCATATCTGGTGGCTCATGGCGGTCGGCTTCGTCGGCATGATCGGCTCC 
TTCATCGTGCGCAGCTACAACCAGGACGTCGACTACTACGTCCAGCCCGAAGAGATCGAGAAGATCGAAAGC 
GCCCGTTTCCAGCAACTCGCCAAGCAGGTCTGA