ViewGene
Locus Name:
GeneID:
Location:

GeneID:3113
TrivialName: PA14_03870
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03870
PAO1 Ortholog Locus:PA0297
Sequence Length:753
Protein Length:250
Start: 348859
Stop: 349611
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03686.1|AE004467_8 (AE004467) probable glutamine amidotransferase [Pseudomonas aeruginosa PAO1]
Identities = 250/250 (100%), Positives = 250/250 (100%)
Sequence:
ATGTCTCGCCTGCCGTTAATCGGCGTCACCGCCTGCACCAAGCAGATCGGTCTTCATCCCTACCACATTGCC
GGCGACAAATATCTGCGTGCCGTCGTCAACGGCGCTGGCGGTCTCCCGCTGATCATTCCTGCGCTGGGCGAG
TCGATCGACCAGGCAGCCCTGCTGGACAGCGTCGACGGCTTGCTCTTCACTGGCTCGCCGTCGAACGTCGAG
CCCCGTCATTATAGTGGACCGGCGAGCGAGCCCGGCACCCTCCACGACTCCGACCGCGACGCCACCACGCTG
CCGCTCGTCCGCGCCGCCATCGACGCCGGCATTCCGGTCCTCGGCATCTGCCGCGGTTTCCAGGAAATGAAC
GTGGCTTTCGGCGGTAGCCTGCACCAGAAGGTGCACGAGGTCGGAACCTTCATGGACCACCGCGAGCCGGCC
GACCAGCCGCTCGAGGTTCAGTACGCGCCGCGCCATGCCATGCATGTGCAGCCGGGCGGAGTGCTCGCCGGC
ATCGGCTTGCCGAGCGAGTTCCAGGTCAATTCCATCCATGGCCAGGGCGTTGATCGTCTGGCGCCGGGCCTT
CGAGTAGAAGCACTGGCGCCTGACGGGTTGGTCGAAGCCATCTCCGTAGAAGGTGCAAAAGCCTTCGCTCTT
GGTGTGCAATGGCACCCGGAGTGGCAGGTTCTGACGAACCCCAACTATCTCGCTATCTTCCAGGCTTTCGGT
AAGGCCTGCAGCAAGAGGGCGGGGCAACGTTGA
Translation:
MSRLPLIGVTACTKQIGLHPYHIAGDKYLRAVVNGAGGLPLIIPALGESIDQAALLDSVDGLLFTGSPSNVE
PRHYSGPASEPGTLHDSDRDATTLPLVRAAIDAGIPVLGICRGFQEMNVAFGGSLHQKVHEVGTFMDHREPA
DQPLEVQYAPRHAMHVQPGGVLAGIGLPSEFQVNSIHGQGVDRLAPGLRVEALAPDGLVEAISVEGAKAFAL
GVQWHPEWQVLTNPNYLAIFQAFGKACSKRAGQR*
AnnotationID:3121GeneID:3113
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 07:07:40
Gene Name:spuA
Confidence Code:1
GeneProduct:glutamine amidotransferase
Cell Localization:()
Synonyms:guaA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2071
Secondary Functional Category(ies):
EC Number:6.3.5.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56395.1| (AE016866) glutamine amidotransferase, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 254

 Score =  300 bits (767), Expect = 7e-81
 Identities = 150/250 (60%), Positives = 181/250 (72%), Gaps = 2/250 (0%)

>gb|AAM41625.1| (AE012342) glutamine amidotransferase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 251

 Score =  267 bits (683), Expect = 4e-71
 Identities = 134/250 (53%), Positives = 168/250 (67%), Gaps = 1/250 (0%)










 
Structural Features:
COG2071, Predicted glutamine amidotransferases
CD-Length = 243 residues,  99.6% aligned
Score =  282 bits (722), Expect = 3e-77
Identities = 117/250 (46%), Positives = 155/250 (62%), Gaps = 12/250 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6229




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23371




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33648




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3113_cdd.html


    		




 
URL:

















    ReferenceID:35433




  
Author/Investigator(s):
        Lu CD, Itoh Y, Nakada Y, Jiang Y.





  
Title:
        Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.




  
PubMed:
        12081945


MedLine:
        




    
Source:
J Bacteriol. 2002 Jul;184(14):3765-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12081945









           
Homologs By Global Alignment 

           

       



     
Gene ID:3113
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5009
                           PA0297_tr
                           translation of PA0297
                           250
                           99.99
                           99.99
                           0
                           1310.0
     

     
     
140993
                           gb|AAG03686.1|AE004467_8
                           (AE004467) probable glutamine amidotransferase [Pseudomonas aeruginosa PAO1]
                           250
                           99.99
                           99.99
                           0
                           1310.0
     

     
     
140994
                           gb|AAN67792.1|AE016410_8
                           (AE016782) conserved hypothetical protein [Pseudomonas putida KT2440]
                           257
                           67.70
                           78.98
                           7
                           935.0
     

     
     
140995
                           gb|AAN54335.1|AE015570_9
                           (AE015570) conserved hypothetical protein [Shewanella oneidensis MR-1]
                           253
                           63.63
                           75.09
                           3
                           818.0
     

     
     
140996
                           gb|AAO56395.1|
                           (AE016866) glutamine amidotransferase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           256
                           58.59
                           70.70
                           8
                           769.0
     

     






      

          
GC ORFID: 46115How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0297Glimmer Score: 

          
Start: 6150201Stop: 6150953

          
Length: 753


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0297 translation of PA0297Homolog Bit Score 509.0

          
Other Homologs:  t_PA0297 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTCGCCTGCCGTTAATCGGCGTCACCGCCTGCACCAAGCAGATCGGTCTTCATCCCTACCACATTGCC 
GGCGACAAATATCTGCGTGCCGTCGTCAACGGCGCTGGCGGTCTCCCGCTGATCATTCCTGCGCTGGGCGAG 
TCGATCGACCAGGCAGCCCTGCTGGACAGCGTCGACGGCTTGCTCTTCACTGGCTCGCCGTCGAACGTCGAG 
CCCCGTCATTATAGTGGACCGGCGAGCGAGCCCGGCACCCTCCACGACTCCGACCGCGACGCCACCACGCTG 
CCGCTCGTCCGCGCCGCCATCGACGCCGGCATTCCGGTCCTCGGCATCTGCCGCGGTTTCCAGGAAATGAAC 
GTGGCTTTCGGCGGTAGCCTGCACCAGAAGGTGCACGAGGTCGGAACCTTCATGGACCACCGCGAGCCGGCC 
GACCAGCCGCTCGAGGTTCAGTACGCGCCGCGCCATGCCATGCATGTGCAGCCGGGCGGAGTGCTCGCCGGC 
ATCGGCTTGCCGAGCGAGTTCCAGGTCAATTCCATCCATGGCCAGGGCGTTGATCGTCTGGCGCCGGGCCTT 
CGAGTAGAAGCACTGGCGCCTGACGGGTTGGTCGAAGCCATCTCCGTAGAAGGTGCAAAAGCCTTCGCTCTT 
GGTGTGCAATGGCACCCGGAGTGGCAGGTTCTGACGAACCCCAACTATCTCGCTATCTTCCAGGCTTTCGGT 
AAGGCCTGCAGCAAGAGGGCGGGGCAACGTTGA