ViewGene
Locus Name:
GeneID:
Location:

GeneID:3109
TrivialName: PA14_56570
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56570
PAO1 Ortholog Locus:PA4351
Sequence Length:777
Protein Length:258
Start: 5043095
Stop: 5043871
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07739.1|AE004851_7 (AE004851) probable acyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 258/258 (100%), Positives = 258/258 (100%)
Sequence:
ATGGCGCGGCTGCGCCTGCTGCTGCGCAGCGCCCGCCTGCTCGGGCTGGTCGCGCTCGGACTGGGCCTGGCC
GCTTGGGTCAGCCTGCGCGAGCGGCTGCCCGGCGCCGACGTCACGCCGCTGCGCCAGCGCCTGACCCGCTGG
TGGCTGGCGCGCCTCTGCGCGGCCCTGCCCTTCGAGGTCAGGGTCAGCGGCGAAGCGCCCCGGCAACCCATG
CTGTGGGTAGCCAACCATGTGTCCTGGACCGATATCCCGCTGCTCGGCGCCCTGGCGCCGCTGACCTTCCTG
TCCAAGGCCGAGGTGCGTGCCTGGCCGCTGGCCGGCTGGCTGGCGGAAAAGGCCGGCACCCTGTTCATCCGC
CGCGGCTCGGGCGACAGCCGGCTGATCAACCAGCGCTTGGCCGAACAGCTGCACCGCGGACGCAACCTGCTG
ATCTTCCCGGAAGGGACCACCACCAACGGCGAAAGCCTGCGCACCTTCCACGGTCGCCTGATGGCCAGCGCC
CTGGAAGCCGGCGTGGCGGTGCAGCCGGTGGCGATCAGCTACCGCCGCGACGGCGTGCCCGATGCGCAGGCG
CCGTTCATCGGCGACGACGACCTGCTCAGCCACCTCGGCCGCCTGCTGCGCGGCGAGCGCGGCAGCGTGCAT
ATCCAACTGCTGGAACCGATCCCCAGCCAGGGCCTGGACCGCGCCGAACTGGCCCGCCAGGCCCAGCAGGCG
GTGCGCCTGGCGCTGTTCGGCACTGCCGCCCCTACGCAAACCCGGCGCGCCGCCTAG
Translation:
MARLRLLLRSARLLGLVALGLGLAAWVSLRERLPGADVTPLRQRLTRWWLARLCAALPFEVRVSGEAPRQPM
LWVANHVSWTDIPLLGALAPLTFLSKAEVRAWPLAGWLAEKAGTLFIRRGSGDSRLINQRLAEQLHRGRNLL
IFPEGTTTNGESLRTFHGRLMASALEAGVAVQPVAISYRRDGVPDAQAPFIGDDDLLSHLGRLLRGERGSVH
IQLLEPIPSQGLDRAELARQAQQAVRLALFGTAAPTQTRRAA*
AnnotationID:3117GeneID:3109
AnnotatorUID: diggins
Modification Date/Time: 2005-05-01 08:08:07
Gene Name:
Confidence Code:3
GeneProduct:putative acyltransferase
Cell Localization:()
Synonyms:PA4351
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0204,smart00563,pfam01553
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57804.1| (AE016871) acyltransferase domain protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 268

 Score =  312 bits (800), Expect = 1e-84
 Identities = 161/247 (65%), Positives = 190/247 (76%)

>gb|AAN66548.1|AE016283_7 (AE016777) acyltransferase [Pseudomonas putida KT2440]
          Length = 262

 Score =  296 bits (758), Expect = 8e-80
 Identities = 159/262 (60%), Positives = 195/262 (74%), Gaps = 4/262 (1%)

>dbj|BAC75022.1| (AP005050) putative 1-acylglycerol-3-phosphate O-acyltransferase
           [Streptomyces avermitilis MA-4680]
          Length = 299

 Score =  140 bits (354), Expect = 6e-33
 Identities = 102/250 (40%), Positives = 137/250 (54%), Gaps = 17/250 (6%)






 
Structural Features:
COG0204, PlsC, 1-acyl-sn-glycerol-3-phosphate acyltransferase 
[Lipid metabolism]
CD-Length = 255 residues,  97.3% aligned
Score =  111 bits (279), Expect = 1e-25
Identities = 79/248 (31%), Positives = 101/248 (40%), Gaps = 10/248 (4%)

smart00563, PlsC, Phosphate acyltransferases
CD-Length = 118 residues,  99.2% aligned
Score = 85.8 bits (212), Expect = 1e-17
Identities = 47/117 (40%), Positives = 57/117 (48%), Gaps = 6/117 (5%)

pfam01553, Acyltransferase, Acyltransferase.
CD-Length = 132 residues,  99.2% aligned
Score = 83.5 bits (206), Expect = 4e-17
Identities = 47/131 (35%), Positives = 60/131 (45%), Gaps = 10/131 (7%)







   
Genomic Context:






   
Comment:


















    ReferenceID:6221




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17104




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3109_cdd.html


    		




 
URL:

















    ReferenceID:23363




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3109
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5005
                           PA4351_tr
                           translation of PA4351
                           258
                           99.99
                           99.99
                           0
                           1310.0
     

     
     
140940
                           gb|AAG07739.1|AE004851_7
                           (AE004851) probable acyltransferase [Pseudomonas aeruginosa PAO1]
                           258
                           99.99
                           99.99
                           0
                           1310.0
     

     
     
140941
                           gb|AAO57804.1|
                           (AE016871) acyltransferase domain protein [Pseudomonas syringae pv. tomato str. DC3000]
                           268
                           61.94
                           72.76
                           10
                           797.0
     

     
     
140942
                           gb|AAN66548.1|AE016283_7
                           (AE016777) acyltransferase [Pseudomonas putida KT2440]
                           262
                           60.68
                           74.42
                           4
                           761.0
     

     
     
140943
                           dbj|BAC75022.1|
                           (AP005050) putative 1-acylglycerol-3-phosphate O-acyltransferase [Streptomyces avermitilis MA-4680]
                           319
                           34.16
                           45.14
                           81
                           373.0
     

     






      

          
GC ORFID: 46111How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4351Glimmer Score: 

          
Start: 4306771Stop: 4307547

          
Length: 777


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4351 translation of PA4351Homolog Bit Score 509.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene1971 (ORF: BLASTX 310.0), t_PA4351 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGCGGCTGCGCCTGCTGCTGCGCAGCGCCCGCCTGCTCGGGCTGGTCGCGCTCGGACTGGGCCTGGCC 
GCTTGGGTCAGCCTGCGCGAGCGGCTGCCCGGCGCCGACGTCACGCCGCTGCGCCAGCGCCTGACCCGCTGG 
TGGCTGGCGCGCCTCTGCGCGGCCCTGCCCTTCGAGGTCAGGGTCAGCGGCGAAGCGCCCCGGCAACCCATG 
CTGTGGGTAGCCAACCATGTGTCCTGGACCGATATCCCGCTGCTCGGCGCCCTGGCGCCGCTGACCTTCCTG 
TCCAAGGCCGAGGTGCGTGCCTGGCCGCTGGCCGGCTGGCTGGCGGAAAAGGCCGGCACCCTGTTCATCCGC 
CGCGGCTCGGGCGACAGCCGGCTGATCAACCAGCGCTTGGCCGAACAGCTGCACCGCGGACGCAACCTGCTG 
ATCTTCCCGGAAGGGACCACCACCAACGGCGAAAGCCTGCGCACCTTCCACGGTCGCCTGATGGCCAGCGCC 
CTGGAAGCCGGCGTGGCGGTGCAGCCGGTGGCGATCAGCTACCGCCGCGACGGCGTGCCCGATGCGCAGGCG 
CCGTTCATCGGCGACGACGACCTGCTCAGCCACCTCGGCCGCCTGCTGCGCGGCGAGCGCGGCAGCGTGCAT 
ATCCAACTGCTGGAACCGATCCCCAGCCAGGGCCTGGACCGCGCCGAACTGGCCCGCCAGGCCCAGCAGGCG 
GTGCGCCTGGCGCTGTTCGGCACTGCCGCCCCTACGCAAACCCGGCGCGCCGCCTAG