ViewGene
Locus Name:
GeneID:
Location:

GeneID:3105
TrivialName: PA14_28570
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_28570
PAO1 Ortholog Locus:PA2749
Sequence Length:714
Protein Length:237
Start: 2463071
Stop: 2462358
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06137.1|AE004703_3 (AE004703) DNA-specific endonuclease I [Pseudomonas aeruginosa PAO1]
Identities = 237/237 (100%), Positives = 237/237 (100%)
Sequence:
ATGCTTTCCCGTTTGTTTGTAGGCCTTTTCGCCCTCCTCCTCGCCAGCGCCGGCCAGGCCAACGCCCCGCGC
ACGTTCTCCGAAGCGAAGAAAATCGGCTGGAAGCTCTACGCCCGGCAATCCGTGGAGTTCTACTGCGGCTGT
CGCTACTCGGGAAATCGTGTCGACCTCGCCAGTTGCGGCTACAAGCCGAGGAAGAACGCCAACCGCGCGCGG
CGCATCGAGTGGGAACACATCGTTCCGGCCTGGGTGATCGGTCACCAGCGACGCTGCTGGCAGAACGGCGGG
CGGAGCAACTGCGCGAGGCACGACAGCACCTACCAGCGCGCCGAGGCCGACCTGCACAACCTGGTGCCGAGC
ATCGGCGAGGTCAACGGCGACCGCAGCAACTTCGGCTTCGGCTGGCTGCCGCAGAAACCCCAGCAATACGGC
GCCTGCCCGATGGTGGTGGACTTCAAGGCGCGCAAGGCGATGCCACGCCCGCAGATTCGCGGGATGATCGCC
CGCACCTACTTCTACATGAGCGACCGCTACGGTCTGCGCCTGTCGAAGCAGGATCGCCAGCTCTACAACGCC
TGGCACAAGCAGTACCCGCCACAGACCTGGGAGCGCCAGCGCAACCACATGGTCGGCTGCGTGATGGGCTGG
GGCAATCCCTATGTCGGCCCGGTGGACCTGGCAACCTGCAGCAACCTATCCTCGCGTCGACGCTGA
Translation:
MLSRLFVGLFALLLASAGQANAPRTFSEAKKIGWKLYARQSVEFYCGCRYSGNRVDLASCGYKPRKNANRAR
RIEWEHIVPAWVIGHQRRCWQNGGRSNCARHDSTYQRAEADLHNLVPSIGEVNGDRSNFGFGWLPQKPQQYG
ACPMVVDFKARKAMPRPQIRGMIARTYFYMSDRYGLRLSKQDRQLYNAWHKQYPPQTWERQRNHMVGCVMGW
GNPYVGPVDLATCSNLSSRRR*
AnnotationID:3113GeneID:3105
AnnotatorUID: diggins
Modification Date/Time: 2005-04-05 08:08:30
Gene Name:endA
Confidence Code:2
GeneProduct:DNA-specific endonuclease I
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2356,pfam04231
Secondary Functional Category(ies):
EC Number:3.1.21.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68063.1|AE016438_9 (AE016783) endonuclease I [Pseudomonas putida KT2440]
          Length = 230

 Score =  363 bits (932), Expect = e-100
 Identities = 165/225 (73%), Positives = 181/225 (80%)

>gb|AAO55875.1| (AE016864) endonuclease I [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 233

 Score =  351 bits (900), Expect = 2e-96
 Identities = 163/232 (70%), Positives = 180/232 (77%), Gaps = 3/232 (1%)








 
Structural Features:
COG2356, EndA, Endonuclease I [DNA replication, recombination, and repair]
CD-Length = 237 residues, 100.0% aligned
Score =  310 bits (795), Expect = 1e-85
Identities = 129/239 (53%), Positives = 154/239 (64%), Gaps = 9/239 (3%)

pfam04231, Endonuclease I
CD-Length = 207 residues,  98.6% aligned
Score =  299 bits (767), Expect = 2e-82
Identities = 120/204 (58%), Positives = 132/204 (64%), Gaps = 4/204 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6213




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23355




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36284




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3105_cdd.html


    		




 
URL:

















    ReferenceID:36391




  
Author/Investigator(s):
        Puyet A, Greenberg B, Lacks SA.





  
Title:
        Genetic and structural characterization of endA. A membrane-bound nuclease required for transformation of Streptococcus pneumoniae.




  
PubMed:
        2359120


MedLine:
        




    
Source:
J Mol Biol. 1990 Jun 20;213(4):727-38.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2359120

















    ReferenceID:36392




  
Author/Investigator(s):
        Jekel M, Wackernagel W.





  
Title:
        Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome.




  
PubMed:
        8113204


MedLine:
        




    
Source:
J Bacteriol. 1994 Mar;176(5):1550-1.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8113204









           
Homologs By Global Alignment 

           

       



     
Gene ID:3105
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4996
                           PA2749_tr
                           translation of PA2749
                           237
                           99.99
                           99.99
                           0
                           1311.0
     

     
     
140742
                           gb|AAG06137.1|AE004703_3
                           (AE004703) DNA-specific endonuclease I [Pseudomonas aeruginosa PAO1]
                           237
                           99.99
                           99.99
                           0
                           1311.0
     

     
     
140743
                           gb|AAN68063.1|AE016438_9
                           (AE016783) endonuclease I [Pseudomonas putida KT2440]
                           237
                           70.04
                           76.79
                           7
                           932.0
     

     
     
140744
                           gb|AAO55875.1|
                           (AE016864) endonuclease I [Pseudomonas syringae pv. tomato str. DC3000]
                           240
                           67.91
                           75.00
                           10
                           903.0
     

     
     
140750
                           emb|CAD02917.1|
                           (AL627277) endonuclease I [Salmonella enterica subsp. enterica serovar Typhi]
                           244
                           51.63
                           61.88
                           16
                           602.0
     

     






      

          
GC ORFID: 46107How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2749Glimmer Score: 

          
Start: 2490855Stop: 2491568

          
Length: 714


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2749 translation of PA2749Homolog Bit Score 509.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene328 (ORF: BLASTX 355.0), t_PA2749 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTTTCCCGTTTGTTTGTAGGCCTTTTCGCCCTCCTCCTCGCCAGCGCCGGCCAGGCCAACGCCCCGCGC 
ACGTTCTCCGAAGCGAAGAAAATCGGCTGGAAGCTCTACGCCCGGCAATCCGTGGAGTTCTACTGCGGCTGT 
CGCTACTCGGGAAATCGTGTCGACCTCGCCAGTTGCGGCTACAAGCCGAGGAAGAACGCCAACCGCGCGCGG 
CGCATCGAGTGGGAACACATCGTTCCGGCCTGGGTGATCGGTCACCAGCGACGCTGCTGGCAGAACGGCGGG 
CGGAGCAACTGCGCGAGGCACGACAGCACCTACCAGCGCGCCGAGGCCGACCTGCACAACCTGGTGCCGAGC 
ATCGGCGAGGTCAACGGCGACCGCAGCAACTTCGGCTTCGGCTGGCTGCCGCAGAAACCCCAGCAATACGGC 
GCCTGCCCGATGGTGGTGGACTTCAAGGCGCGCAAGGCGATGCCACGCCCGCAGATTCGCGGGATGATCGCC 
CGCACCTACTTCTACATGAGCGACCGCTACGGTCTGCGCCTGTCGAAGCAGGATCGCCAGCTCTACAACGCC 
TGGCACAAGCAGTACCCGCCACAGACCTGGGAGCGCCAGCGCAACCACATGGTCGGCTGCGTGATGGGCTGG 
GGCAATCCCTATGTCGGCCCGGTGGACCTGGCAACCTGCAGCAACCTATCCTCGCGTCGACGCTGA