ViewGene
Locus Name:
GeneID:
Location:

GeneID:31
TrivialName: PA14_40670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_40670
PAO1 Ortholog Locus:PA1843
Sequence Length:3705
Protein Length:1234
Start: 3631084
Stop: 3634788
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05232.1|AE004610_5 (AE004610) methionine synthase [Pseudomonas aeruginosa PAO1]
Identities = 1233/1234 (99%), Positives = 1233/1234 (99%)
Sequence:
ATGTCCAGCCCGCTCACCGATCGCAGCGCCCGCCTGCAAGCCCTCCAGCACGCCCTCAGGGAACGTATCCTG
ATCCTCGATGGCGGCATGGGCACCATGATCCAGAGCTACAAGCTGGAAGAAGCCGACTACCGCGGCGAGCGC
TTCGCCGACTGGCCGAGCGACGTGAAAGGCAACAACGACCTCTTGCTGCTCAGCCGCCCGGACGTGATCCAG
GCCATCGAGAATGCCTACCTCGACGCCGGCGCCGACATCCTCGAGACCAATACCTTCAACGCCACCCAGGTG
TCCCAGGCCGACTACGGCATGCAGTCGCTGGCCTACGAACTCAACGTCGAAGGGGCGCGCCTGGCTCGCCAG
GTGGCGGACGCGAAGACCGCCGAGACCCCGGACAAGCCGCGCTTCGTCGCCGGCGTGCTCGGCCCGACCAGC
CGCACCTGCTCGATTTCCCCGGACGTGAACAACCCCGGCTACCGCAACGTCACCTTCGACGAGCTGGTGGAG
AACTACGTCGAGGCGACCCGCGGCCTGATCGAGGGCGGCGCCGACCTGATCCTGATCGAGACCATCTTCGAC
ACCCTCAATGCCAAGGCGGCGATATTCGCCGTCCAGGGCGTGTTCGAGGAACTCGGCGTGGAGCTGCCGATC
ATGATCTCCGGGACCATCACCGACGCCTCCGGCCGCACCCTGTCGGGCCAGACCACCGAGGCCTTCTGGAAC
TCGGTGCGGCATGCCCGGCCGATCTCGGTAGGCCTGAACTGCGCCCTCGGCGCCAAGGAATTGCGGCCGTAC
ATCGAGGAACTGTCGACCAAGGCCGACACTCATGTCTCGGCCCACCCCAACGCCGGCCTGCCGAACGCCTTC
GGCGAATACGACGAATCGCCGGCGGAAATGGCCGTGGTGGTCGAGGAATTCGCCGCCGCCGGCTTCCTCAAT
ATCGTCGGCGGCTGCTGCGGCACCACCCCGGCGCACATCGAGGCGATCGCCAAGGCAGTGGCCAAGTACCCG
CCGCGGGCCATCCCGGAGATTCCCCGGGCCTGTCGCCTGTCCGGCCTGGAGCCGTTCACCATCGACCGCAGC
TCGCTGTTCGTCAACGTCGGCGAGCGCACCAACATCACCGGTTCGGCCAAGTTCGCCCGGCTGATCCGCGAG
GAAAACTACGCGGAAGCTCTCGAGGTCGCCCAGCAGCAGGTGGAAGCCGGCGCCCAGGTGATCGACATCAAC
ATGGACGAAGGCATGCTGGACTCGAAGGCGGCCATGGTCACCTTCCTCAACCTGATCGCCTCCGAGCCCGAC
ATCTCGCGCGTGCCGATCATGATCGACTCCTCCAAGTGGGAAGTGATCGAGGCCGGCCTGAAGTGCATCCAG
GGCAAGGGCATCGTCAACTCGATCTCGATGAAGGAAGGCGTCGAGGCCTTCAAGCACCATGCCCGCCTGTGC
AAGCGCTACGGCGCCGCGGTGGTGGTGATGGCCTTCGACGAGGACGGCCAGGCCGACACCCAGGCGCGCAAG
GAAGAAATCTGCAAGCGCTCCTACGACATCCTGGTCGACGAAGTCGGCTTCCCACCGGAAGACATCATCTTC
GATGCGAACATCTTCGCCATCGCCACCGGCATCGAGGAACACAACAACTACGCGGTCGATTTCATCAACGCC
TGCGCCTACATCCGCGACAACCTCCCCTACGCCCTGAGTTCGGGCGGGGTGTCCAACGTGTCCTTCTCGTTC
CGCGGCAACAACCCGGTACGCGAGGCGATCCACTCGGTGTTCCTCTACTACGCGATCCGCAACGGCCTGACC
ATGGGCATCGTCAACGCCGGCCAGCTGGAAATCTACGACGAGATTCCGAAAGCGCTGCGCGACCGGGTCGAG
GACGTGGTGCTCAACCGCACGCCCGAGGCCACCGAGGCCCTGCTGGCGATCGCCGACGACTACAAGGGCGGC
GGCGCGGTCAAGGAGGCCGAGGACGAGGAATGGCGCAGCTACAGTGTCGAGAAGCGCCTCGAGCATGCGCTG
GTCAAGGGCATCACCACCTGGATCGTCGAGGACACCGAGGAATGCCGCCAGCAGTGTGCGCGTCCCATCGAG
GTCATCGAAGGTCCGCTGATGTCCGGGATGAACGTGGTCGGCGACCTGTTCGGCGCCGGCAAGATGTTCCTC
CCGCAGGTGGTCAAGTCCGCGCGAGTGATGAAGCAGGCGGTGGCCCACCTGATTCCCTTCATCGAGGCGGAG
AAAGGCGACAAGCCGGAAGCCAAGGGCAAGATCCTGATGGCCACGGTGAAGGGCGACGTGCACGACATCGGC
AAGAACATCGTCGGCGTGGTGCTCGGCTGCAACGGCTATGACGTGGTCGACCTCGGCGTGATGGTGCCGGCG
GAGAAGATCCTGCAGACCGCCATCGCCGAGAAATGCGACATCATCGGCCTGTCTGGCCTGATCACGCCGTCG
CTGGACGAGATGGTCCACGTCGCCAAGGAAATGCAGCGGCAGAATTTCCAGTTGCCGCTGATGATCGGCGGC
GCCACTACCTCGAAGGCGCATACCGCGGTGAAGATCGATCCGCAGTACAGCAACGACGCGGTGGTCTACGTC
ACCGACGCCTCGCGCGCGGTAGGCGTGGCCACCAGCCTGCTGTCCAAGGAGCTGAAGGCCGACTACGTGGCC
CGCACCCGCGCCGACTACGCGGTGGTCCGCGAACGCACGGCCAACCGCAGCGCCCGCACCGAGCGGCTGAGC
TACGAACAGGCGATCGCCAACAAGCCGGCGTTCGACTGGGCCGGCTACCAGGCGCCGACGCCTTCCTTCACC
GGCGTCAGGGTGCTCGACGAGATCGACCTCGCGGTGCTCGCCGAGTACATCGACTGGACGCCGTTCTTCATT
TCCTGGGACCTGGCCGGCAAGTACCCGCGCATCCTCACCGACGAGGTGGTCGGCgagGCCGCCACCTCGTTG
TTCAATGACGCCCAGGCGATGCTGAAGAAGCTGATCGACGAGAAGCTGATCAAGGCCCGTGCGGTGTTCGGC
TTCTGGCCGGCCAACCAGGTCGAGCACGACGACCTGGAGGTCTACGGCGCCGAtgGCGAGACCCTCGCCACc
ctGCACCACCTGCGGCAGCAGACGATCAAGCCGGACGGCAAGCCGAACCTGTCGCTGGCCGATTTCGTCGCG
CCGAAGGAAAGCGGCGTGCGCGACtaCATCGGCGGCTTCATCACCACCGCCGGGATCGGCGCCGAGGAAGTG
GCCAAGGCGTACGAAGCCAAGGGCGACGACTACAACAGCATCATGGTCAAGGCGCTCGCCGACCGCCTCGCC
GAAGCCTGCGCCGAGTGGCTGCACGAGCGGGTGCGCAAGGAGTACTGGGGCTACGCCCGCGACGAACACCTC
GACAACGAGGCCTTGATCAAGGAGCAATACGTCGGCATCCGCCCGGCACCGGGCTACCCGGCCTGCCCCGAC
CATACCGAGAAAGGCACTCTGTTCGAACTGCTCGATCCGCAGGGCCTGTCCGGCGTCAGCCTGACCGAGCAC
TACGCGATGTTCCCGGCCGCGGCGGTCAGCGGTTGGTATTTCGCCCACCCGCAGGCGCAGTACTTCGCGGTC
GGCAAGATCGACAAGGACCAGGTGGAACGCTACAGCCAGCGCAAGGGCCAGGAAGCCAGCGTCAGCGAGCGC
TGGCTGGCGCCGAACCTTGGCTACGATGACTGA
Translation:
MSSPLTDRSARLQALQHALRERILILDGGMGTMIQSYKLEEADYRGERFADWPSDVKGNNDLLLLSRPDVIQ
AIENAYLDAGADILETNTFNATQVSQADYGMQSLAYELNVEGARLARQVADAKTAETPDKPRFVAGVLGPTS
RTCSISPDVNNPGYRNVTFDELVENYVEATRGLIEGGADLILIETIFDTLNAKAAIFAVQGVFEELGVELPI
MISGTITDASGRTLSGQTTEAFWNSVRHARPISVGLNCALGAKELRPYIEELSTKADTHVSAHPNAGLPNAF
GEYDESPAEMAVVVEEFAAAGFLNIVGGCCGTTPAHIEAIAKAVAKYPPRAIPEIPRACRLSGLEPFTIDRS
SLFVNVGERTNITGSAKFARLIREENYAEALEVAQQQVEAGAQVIDINMDEGMLDSKAAMVTFLNLIASEPD
ISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISMKEGVEAFKHHARLCKRYGAAVVVMAFDEDGQADTQARK
EEICKRSYDILVDEVGFPPEDIIFDANIFAIATGIEEHNNYAVDFINACAYIRDNLPYALSSGGVSNVSFSF
RGNNPVREAIHSVFLYYAIRNGLTMGIVNAGQLEIYDEIPKALRDRVEDVVLNRTPEATEALLAIADDYKGG
GAVKEAEDEEWRSYSVEKRLEHALVKGITTWIVEDTEECRQQCARPIEVIEGPLMSGMNVVGDLFGAGKMFL
PQVVKSARVMKQAVAHLIPFIEAEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDVVDLGVMVPA
EKILQTAIAEKCDIIGLSGLITPSLDEMVHVAKEMQRQNFQLPLMIGGATTSKAHTAVKIDPQYSNDAVVYV
TDASRAVGVATSLLSKELKADYVARTRADYAVVRERTANRSARTERLSYEQAIANKPAFDWAGYQAPTPSFT
GVRVLDEIDLAVLAEYIDWTPFFISWDLAGKYPRILTDEVVGEAATSLFNDAQAMLKKLIDEKLIKARAVFG
FWPANQVEHDDLEVYGADGETLATLHHLRQQTIKPDGKPNLSLADFVAPKESGVRDYIGGFITTAGIGAEEV
AKAYEAKGDDYNSIMVKALADRLAEACAEWLHERVRKEYWGYARDEHLDNEALIKEQYVGIRPAPGYPACPD
HTEKGTLFELLDPQGLSGVSLTEHYAMFPAAAVSGWYFAHPQAQYFAVGKIDKDQVERYSQRKGQEASVSER
WLAPNLGYDD*
AnnotationID:41GeneID:31
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 14:02:27
Gene Name:metH
Confidence Code:2
GeneProduct:methionine synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:5-methyltetrahydrofolate--homocysteine methyltransferase
Functional Category Confidence Code:5
COGs:COG1410,COG0646,pfam02965
Secondary Functional Category(ies):
EC Number:2.1.1.13
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67988.1|AE016431_4 (AE016782) 5-methyltetrahydrofolate--homocysteine methyltransferase
            [Pseudomonas putida KT2440]
          Length = 1235

 Score = 2185 bits (5661), Expect = 0.0
 Identities = 1089/1234 (88%), Positives = 1158/1234 (93%), Gaps = 5/1234 (0%)

>gb|AAO56233.1| (AE016865) 5-methyltetrahydrofolate--homocysteine methyltransferase
            [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 1239

 Score = 2122 bits (5498), Expect = 0.0
 Identities = 1058/1239 (85%), Positives = 1134/1239 (91%), Gaps = 9/1239 (0%)

>gb|AAL23012.1| (AE008895) B12-dependent homocysteine-N5-methyltetrahydrofolate
            transmethylase [Salmonella typhimurium LT2]
          Length = 1256

 Score = 1640 bits (4248), Expect = 0.0
 Identities = 837/1229 (68%), Positives = 986/1229 (80%), Gaps = 9/1229 (0%)






 
Structural Features:
COG1410, MetH, Methionine synthase I, cobalamin-binding domain 
[Amino acid transport and metabolism]
CD-Length = 842 residues, 100.0% aligned
Score = 1192 bits (3086), Expect = 0.0
Identities = 534/867 (61%), Positives = 623/867 (71%), Gaps = 26/867 (2%)

COG0646, MetH, Methionine synthase I (cobalamin-dependent), methyltransferase domain 
[Amino acid transport and metabolism]
CD-Length = 311 residues, 100.0% aligned
Score =  432 bits (1113), Expect = 1e-121
Identities = 204/330 (61%), Positives = 243/330 (73%), Gaps = 22/330 (6%)

pfam02965, Met_synt_B12, Vitamin B12 dependent methionine synthase, activation domain.
CD-Length = 291 residues,  99.7% aligned
Score =  354 bits (909), Expect = 4e-98
Identities = 165/294 (56%), Positives = 195/294 (66%), Gaps = 11/294 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:65




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17207




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37090




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:31_cdd.html


    		




 
URL:

















    ReferenceID:37791




  
Author/Investigator(s):
        Kasai S, Yamazaki T.





  
Title:
        Identification of the cobalamin-dependent methionine synthase gene, metH, in Vibrio fischeri ATCC 7744 by sequencing using genomic DNA as a template.




  
PubMed:
        11250084


MedLine:
        




    
Source:
Gene. 2001 Feb 21;264(2):281-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11250084

















    ReferenceID:37792




  
Author/Investigator(s):
        Banerjee RV, Johnston NL, Sobeski JK, Datta P, Matthews RG.





  
Title:
        Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain.




  
PubMed:
        2668277


MedLine:
        




    
Source:
J Biol Chem. 1989 Aug 15;264(23):13888-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=89340482&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:31
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
67
                           PA1843_tr
                           translation of PA1843
                           1234
                           99.91
                           99.91
                           0
                           6336.0
     

     
     
11255
                           gb|AAG05232.1|AE004610_5
                           (AE004610) methionine synthase [Pseudomonas aeruginosa PAO1]
                           1234
                           99.91
                           99.91
                           0
                           6336.0
     

     
     
11256
                           gb|AAN67988.1|AE016431_4
                           (AE016782) 5-methyltetrahydrofolate--homocysteine methyltransferase [Pseudomonas putida KT2440]
                           1239
                           87.89
                           93.46
                           9
                           5665.0
     

     
     
11257
                           gb|AAK29461.1|AF363276_1
                           (AF363276) MetH [Pseudomonas putida]
                           1241
                           87.34
                           92.82
                           11
                           5616.0
     

     
     
11258
                           gb|AAO56233.1|
                           (AE016865) 5-methyltetrahydrofolate--homocysteine methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           1243
                           85.11
                           91.23
                           13
                           5504.0
     

     






      

          
GC ORFID: 43033How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1843Glimmer Score: 

          
Start: 1322826Stop: 1319122

          
Length: 3705


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1843 translation of PA1843Homolog Bit Score 2445.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8075 (ORF: BLASTX 2123.0), t_PA1843 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCAGCCCGCTCACCGATCGCAGCGCCCGCCTGCAAGCCCTCCAGCACGCCCTCAGGGAACGTATCCTG 
ATCCTCGATGGCGGCATGGGCACCATGATCCAGAGCTACAAGCTGGAAGAAGCCGACTACCGCGGCGAGCGC 
TTCGCCGACTGGCCGAGCGACGTGAAAGGCAACAACGACCTCTTGCTGCTCAGCCGCCCGGACGTGATCCAG 
GCCATCGAGAATGCCTACCTCGACGCCGGCGCCGACATCCTCGAGACCAATACCTTCAACGCCACCCAGGTG 
TCCCAGGCCGACTACGGCATGCAGTCGCTGGCCTACGAACTCAACGTCGAAGGGGCGCGCCTGGCTCGCCAG 
GTGGCGGACGCGAAGACCGCCGAGACCCCGGACAAGCCGCGCTTCGTCGCCGGCGTGCTCGGCCCGACCAGC 
CGCACCTGCTCGATTTCCCCGGACGTGAACAACCCCGGCTACCGCAACGTCACCTTCGACGAGCTGGTGGAG 
AACTACGTCGAGGCGACCCGCGGCCTGATCGAGGGCGGCGCCGACCTGATCCTGATCGAGACCATCTTCGAC 
ACCCTCAATGCCAAGGCGGCGATATTCGCCGTCCAGGGCGTGTTCGAGGAACTCGGCGTGGAGCTGCCGATC 
ATGATCTCCGGGACCATCACCGACGCCTCCGGCCGCACCCTGTCGGGCCAGACCACCGAGGCCTTCTGGAAC 
TCGGTGCGGCATGCCCGGCCGATCTCGGTAGGCCTGAACTGCGCCCTCGGCGCCAAGGAATTGCGGCCGTAC 
ATCGAGGAACTGTCGACCAAGGCCGACACTCATGTCTCGGCCCACCCCAACGCCGGCCTGCCGAACGCCTTC 
GGCGAATACGACGAATCGCCGGCGGAAATGGCCGTGGTGGTCGAGGAATTCGCCGCCGCCGGCTTCCTCAAT 
ATCGTCGGCGGCTGCTGCGGCACCACCCCGGCGCACATCGAGGCGATCGCCAAGGCAGTGGCCAAGTACCCG 
CCGCGGGCCATCCCGGAGATTCCCCGGGCCTGTCGCCTGTCCGGCCTGGAGCCGTTCACCATCGACCGCAGC 
TCGCTGTTCGTCAACGTCGGCGAGCGCACCAACATCACCGGTTCGGCCAAGTTCGCCCGGCTGATCCGCGAG 
GAAAACTACGCGGAAGCTCTCGAGGTCGCCCAGCAGCAGGTGGAAGCCGGCGCCCAGGTGATCGACATCAAC 
ATGGACGAAGGCATGCTGGACTCGAAGGCGGCCATGGTCACCTTCCTCAACCTGATCGCCTCCGAGCCCGAC 
ATCTCGCGCGTGCCGATCATGATCGACTCCTCCAAGTGGGAAGTGATCGAGGCCGGCCTGAAGTGCATCCAG 
GGCAAGGGCATCGTCAACTCGATCTCGATGAAGGAAGGCGTCGAGGCCTTCAAGCACCATGCCCGCCTGTGC 
AAGCGCTACGGCGCCGCGGTGGTGGTGATGGCCTTCGACGAGGACGGCCAGGCCGACACCCAGGCGCGCAAG 
GAAGAAATCTGCAAGCGCTCCTACGACATCCTGGTCGACGAAGTCGGCTTCCCACCGGAAGACATCATCTTC 
GATGCGAACATCTTCGCCATCGCCACCGGCATCGAGGAACACAACAACTACGCGGTCGATTTCATCAACGCC 
TGCGCCTACATCCGCGACAACCTCCCCTACGCCCTGAGTTCGGGCGGGGTGTCCAACGTGTCCTTCTCGTTC 
CGCGGCAACAACCCGGTACGCGAGGCGATCCACTCGGTGTTCCTCTACTACGCGATCCGCAACGGCCTGACC 
ATGGGCATCGTCAACGCCGGCCAGCTGGAAATCTACGACGAGATTCCGAAAGCGCTGCGCGACCGGGTCGAG 
GACGTGGTGCTCAACCGCACGCCCGAGGCCACCGAGGCCCTGCTGGCGATCGCCGACGACTACAAGGGCGGC 
GGCGCGGTCAAGGAGGCCGAGGACGAGGAATGGCGCAGCTACAGTGTCGAGAAGCGCCTCGAGCATGCGCTG 
GTCAAGGGCATCACCACCTGGATCGTCGAGGACACCGAGGAATGCCGCCAGCAGTGTGCGCGTCCCATCGAG 
GTCATCGAAGGTCCGCTGATGTCCGGGATGAACGTGGTCGGCGACCTGTTCGGCGCCGGCAAGATGTTCCTC 
CCGCAGGTGGTCAAGTCCGCGCGAGTGATGAAGCAGGCGGTGGCCCACCTGATTCCCTTCATCGAGGCGGAG 
AAAGGCGACAAGCCGGAAGCCAAGGGCAAGATCCTGATGGCCACGGTGAAGGGCGACGTGCACGACATCGGC 
AAGAACATCGTCGGCGTGGTGCTCGGCTGCAACGGCTATGACGTGGTCGACCTCGGCGTGATGGTGCCGGCG 
GAGAAGATCCTGCAGACCGCCATCGCCGAGAAATGCGACATCATCGGCCTGTCTGGCCTGATCACGCCGTCG 
CTGGACGAGATGGTCCACGTCGCCAAGGAAATGCAGCGGCAGAATTTCCAGTTGCCGCTGATGATCGGCGGC 
GCCACTACCTCGAAGGCGCATACCGCGGTGAAGATCGATCCGCAGTACAGCAACGACGCGGTGGTCTACGTC 
ACCGACGCCTCGCGCGCGGTAGGCGTGGCCACCAGCCTGCTGTCCAAGGAGCTGAAGGCCGACTACGTGGCC 
CGCACCCGCGCCGACTACGCGGTGGTCCGCGAACGCACGGCCAACCGCAGCGCCCGCACCGAGCGGCTGAGC 
TACGAACAGGCGATCGCCAACAAGCCGGCGTTCGACTGGGCCGGCTACCAGGCGCCGACGCCTTCCTTCACC 
GGCGTCAGGGTGCTCGACGAGATCGACCTCGCGGTGCTCGCCGAGTACATCGACTGGACGCCGTTCTTCATT 
TCCTGGGACCTGGCCGGCAAGTACCCGCGCATCCTCACCGACGAGGTGGTCGGCgagGCCGCCACCTCGTTG 
TTCAATGACGCCCAGGCGATGCTGAAGAAGCTGATCGACGAGAAGCTGATCAAGGCCCGTGCGGTGTTCGGC 
TTCTGGCCGGCCAACCAGGTCGAGCACGACGACCTGGAGGTCTACGGCGCCGAtgGCGAGACCCTCGCCACc 
ctGCACCACCTGCGGCAGCAGACGATCAAGCCGGACGGCAAGCCGAACCTGTCGCTGGCCGATTTCGTCGCG 
CCGAAGGAAAGCGGCGTGCGCGACtaCATCGGCGGCTTCATCACCACCGCCGGGATCGGCGCCGAGGAAGTG 
GCCAAGGCGTACGAAGCCAAGGGCGACGACTACAACAGCATCATGGTCAAGGCGCTCGCCGACCGCCTCGCC 
GAAGCCTGCGCCGAGTGGCTGCACGAGCGGGTGCGCAAGGAGTACTGGGGCTACGCCCGCGACGAACACCTC 
GACAACGAGGCCTTGATCAAGGAGCAATACGTCGGCATCCGCCCGGCACCGGGCTACCCGGCCTGCCCCGAC 
CATACCGAGAAAGGCACTCTGTTCGAACTGCTCGATCCGCAGGGCCTGTCCGGCGTCAGCCTGACCGAGCAC 
TACGCGATGTTCCCGGCCGCGGCGGTCAGCGGTTGGTATTTCGCCCACCCGCAGGCGCAGTACTTCGCGGTC 
GGCAAGATCGACAAGGACCAGGTGGAACGCTACAGCCAGCGCAAGGGCCAGGAAGCCAGCGTCAGCGAGCGC 
TGGCTGGCGCCGAACCTTGGCTACGATGACTGA