ViewGene
Locus Name:
GeneID:
Location:

GeneID:3095
TrivialName: PA14_66900
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66900
PAO1 Ortholog Locus:PA5063
Sequence Length:771
Protein Length:256
Start: 5974053
Stop: 5974823
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08448.1|AE004919_9 (AE004919) ubiquinone biosynthesis methyltransferase UbiE [Pseudomonas aeruginosa PAO1]
Identities = 256/256 (100%), Positives = 256/256 (100%)
Sequence:
ATGAACGATCCCCGCAAAGGCGCCGACGCCGAACCCACCACCCACTTCGGCTACCAGAACGTTCCGGAAAGC
CAGAAGGCGAAGAAGGTCGCCGAGGTCTTCCACTCGGTGGCGGCCAAGTACGACCTGATGAATGACCTGATG
TCCGGCGGCATCCATCGCCTGTGGAAGCGCTTCACCATCGAGCTGTCCGGCGTGCGCAGCGGGAACCGCGTG
CTCGACATCGCCGGCGGCACCGGCGACCTGACCCGGCAGTTCTCGCGCCTGGTCGGCCCGACCGGCGAGGTG
GTGCTGGCCGACATCAACGCCTCGATGCTCAAGGTCGGCCGCGACAAGCTGCTCGACAAGGGCGTCTCCGGC
AACGTCAGCTTCGTCCAGGCGGACGCCGAGAAGCTGCCCTTCCCGGACAACCACTTCGACTGCGTGACCATC
GCCTTCGGCCTGCGCAACGTCACCCACAAGGACGAAGCGATCCGCTCCATGCTGCGCGTGCTCAAGCCCGGT
GGGCGCCTGCTGGTGCTGGAGTTCTCCAAGCCGAGCAGCAACCTGCTGTCCAAGGCCTACGACGCCTATTCG
TTCAGCCTGCTGCCGCTGATGGGCAAGCTGGTCACCAACGACTCCGAGAGCTATCGCTACCTGGCCGAGTCG
ATCCGCATGCACCCCGACCAGGAAACCCTGAAGGCGATGATGGTGGAAGCCGGCTTCGACCGCGTGACCTAC
CACAACATGACCGGCGGCATCGTCGCCCTGCACCGCGGTATCAAGCCTTAA
Translation:
MNDPRKGADAEPTTHFGYQNVPESQKAKKVAEVFHSVAAKYDLMNDLMSGGIHRLWKRFTIELSGVRSGNRV
LDIAGGTGDLTRQFSRLVGPTGEVVLADINASMLKVGRDKLLDKGVSGNVSFVQADAEKLPFPDNHFDCVTI
AFGLRNVTHKDEAIRSMLRVLKPGGRLLVLEFSKPSSNLLSKAYDAYSFSLLPLMGKLVTNDSESYRYLAES
IRMHPDQETLKAMMVEAGFDRVTYHNMTGGIVALHRGIKP*
AnnotationID:3103GeneID:3095
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:17
Gene Name:ubiE
Confidence Code:2
GeneProduct:ubiquinone biosynthesis methyltransferase UbiE
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2226,pfam01209
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:2.1.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58576.1| (AE016874) ubiquinone/menaquinone biosynthesis methlytransferase
           UbiE [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 256

 Score =  469 bits (1207), Expect = e-132
 Identities = 227/256 (88%), Positives = 248/256 (96%)

>gb|AAN70577.1|AE016700_5 (AE016792) ubiquinone biosynthesis methyltransferase [Pseudomonas
           putida KT2440]
          Length = 256

 Score =  463 bits (1191), Expect = e-130
 Identities = 224/256 (87%), Positives = 244/256 (95%)

>emb|CAC93248.1| (AJ414158) ubiquinone/menaquinone biosynthesis methyltransferase
           [Yersinia pestis CO92]
          Length = 251

 Score =  375 bits (963), Expect = e-103
 Identities = 182/243 (74%), Positives = 210/243 (86%)






 
Structural Features:
COG2226, UbiE, Methylase involved in ubiquinone/menaquinone biosynthesis 
[Coenzyme metabolism]
CD-Length = 238 residues, 100.0% aligned
Score =  317 bits (815), Expect = 6e-88
Identities = 146/240 (60%), Positives = 179/240 (74%), Gaps = 3/240 (1%)

pfam01209, Ubie_methyltran, ubiE/COQ5 methyltransferase family.
CD-Length = 233 residues,  98.3% aligned
Score =  317 bits (813), Expect = 9e-88
Identities = 122/230 (53%), Positives = 163/230 (70%), Gaps = 1/230 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:6193




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23335




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34981




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3095_cdd.html


    		




 
URL:

















    ReferenceID:35827




  
Author/Investigator(s):
        Lee PT, Hsu AY, Ha HT, Clarke CF.





  
Title:
        A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene.




  
PubMed:
        9045837


MedLine:
        




    
Source:
J Bacteriol. 1997 Mar;179(5):1748-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9045837









           
Homologs By Global Alignment 

           

       



     
Gene ID:3095
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4986
                           PA5063_tr
                           translation of PA5063
                           256
                           99.99
                           99.99
                           0
                           1316.0
     

     
     
140595
                           gb|AAG08448.1|AE004919_9
                           (AE004919) ubiquinone biosynthesis methyltransferase UbiE [Pseudomonas aeruginosa PAO1]
                           256
                           99.99
                           99.99
                           0
                           1316.0
     

     
     
140596
                           gb|AAO58576.1|
                           (AE016874) ubiquinone/menaquinone biosynthesis methlytransferase UbiE [Pseudomonas syringae pv. tomato str. DC3000]
                           256
                           88.67
                           96.87
                           0
                           1207.0
     

     
     
140597
                           gb|AAD02212.1|
                           (AF042276) o251 homolog [Pseudomonas putida]
                           256
                           88.67
                           95.70
                           0
                           1202.0
     

     
     
140598
                           gb|AAN70577.1|AE016700_5
                           (AE016792) ubiquinone biosynthesis methyltransferase [Pseudomonas putida KT2440]
                           256
                           87.50
                           95.31
                           0
                           1191.0
     

     






      

          
GC ORFID: 46097How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5063Glimmer Score: 

          
Start: 5237737Stop: 5238507

          
Length: 771


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5063 translation of PA5063Homolog Bit Score 511.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3794 (ORF: BLASTX 470.0), t_PA5063 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGATCCCCGCAAAGGCGCCGACGCCGAACCCACCACCCACTTCGGCTACCAGAACGTTCCGGAAAGC 
CAGAAGGCGAAGAAGGTCGCCGAGGTCTTCCACTCGGTGGCGGCCAAGTACGACCTGATGAATGACCTGATG 
TCCGGCGGCATCCATCGCCTGTGGAAGCGCTTCACCATCGAGCTGTCCGGCGTGCGCAGCGGGAACCGCGTG 
CTCGACATCGCCGGCGGCACCGGCGACCTGACCCGGCAGTTCTCGCGCCTGGTCGGCCCGACCGGCGAGGTG 
GTGCTGGCCGACATCAACGCCTCGATGCTCAAGGTCGGCCGCGACAAGCTGCTCGACAAGGGCGTCTCCGGC 
AACGTCAGCTTCGTCCAGGCGGACGCCGAGAAGCTGCCCTTCCCGGACAACCACTTCGACTGCGTGACCATC 
GCCTTCGGCCTGCGCAACGTCACCCACAAGGACGAAGCGATCCGCTCCATGCTGCGCGTGCTCAAGCCCGGT 
GGGCGCCTGCTGGTGCTGGAGTTCTCCAAGCCGAGCAGCAACCTGCTGTCCAAGGCCTACGACGCCTATTCG 
TTCAGCCTGCTGCCGCTGATGGGCAAGCTGGTCACCAACGACTCCGAGAGCTATCGCTACCTGGCCGAGTCG 
ATCCGCATGCACCCCGACCAGGAAACCCTGAAGGCGATGATGGTGGAAGCCGGCTTCGACCGCGTGACCTAC 
CACAACATGACCGGCGGCATCGTCGCCCTGCACCGCGGTATCAAGCCTTAA