ViewGene
Locus Name:
GeneID:
Location:

GeneID:3094
TrivialName: PA14_61660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_61660
PAO1 Ortholog Locus:PA4662
Sequence Length:798
Protein Length:265
Start: 5502854
Stop: 5502057
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08049.1|AE004880_5 (AE004880) glutamate racemase [Pseudomonas aeruginosa PAO1]
Identities = 264/265 (99%), Positives = 264/265 (99%)
Sequence:
ATGGCGGTTGAGTCGGCCGCGGTCGGCGTTTTCGATTCCGGGGTCGGCGGGCTCAGCGTATTGCGTGAGATT
CGTGCGCGCCTGCCATCCGAATCGTTGCTCTACGTCGCCGACAACGCCCATGTGCCCTACGGCGAGAAGAGC
GCGGAGTACATCCGCGAACGCTGCGAGCGGATCGGTGATTTCCTTCTGGAGCAGGGCGCCAAGGCTCTGGTA
CTGGCCTGCAATACCGCGACGGCAGCGGCTGCGGCGGAATTGCGCGAACGTTATCCGCAGGTGCAGCTGGTG
GCCATGGAGCCGGCGGTGAAGCCGGCCGCGGCGGCCACCCGCAACGGTCGGGTCGGCGTCCTCGCCACCACC
GGAACTCTGAAGAGCGCGCGTTTCGCCGCCTTGCTCGACCGCTTCGCCAGCGATGTACAGGTCTTCACCCAG
CCCTGTCCGGGGCTGGTCGAGCGCATCGAAGCCGGCGATCTCTATGGGCCGCAAACCCGGGCACTGCTGGAA
CGCCTGCTGGCACCCATCCTCGAACAGGGCTGCGATACGCTGATCCTCGGCTGTACTCATTATCCCTTCGTC
AAACCCCTGCTGGCCGAGCTGATCCCGGCGGAGATGGCGGTGATCGATACGGGAGCGGCTGTCGCCCGGCAG
CTGGAGCGCGTATTGTCGGCGCGGGCGCTACTGGCCTCTGGCCAGGCCGCGACGCCGCGTTTCTGGACCAGC
GCGCTGCCGGAGGAGATGGAAAGGATCCTGCCGATCCTCTGGGGAAGCCCGGAAAGCGTAGGAAAATTAGTC
GTTTAG
Translation:
MAVESAAVGVFDSGVGGLSVLREIRARLPSESLLYVADNAHVPYGEKSAEYIRERCERIGDFLLEQGAKALV
LACNTATAAAAAELRERYPQVQLVAMEPAVKPAAAATRNGRVGVLATTGTLKSARFAALLDRFASDVQVFTQ
PCPGLVERIEAGDLYGPQTRALLERLLAPILEQGCDTLILGCTHYPFVKPLLAELIPAEMAVIDTGAAVARQ
LERVLSARALLASGQAATPRFWTSALPEEMERILPILWGSPESVGKLVV*
AnnotationID:3102GeneID:3094
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 20:08:54
Gene Name:murI
Confidence Code:2
GeneProduct:glutamate racemase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0796,pfam01177
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:5.1.1.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66361.1|AE016264_5 (AE016776) glutamate racemase [Pseudomonas putida KT2440]
          Length = 265

 Score =  357 bits (915), Expect = 5e-98
 Identities = 179/260 (68%), Positives = 207/260 (79%)

>gb|AAO54641.1| (AE016859) glutamate racemase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 275

 Score =  334 bits (856), Expect = 4e-91
 Identities = 169/248 (68%), Positives = 196/248 (79%)

>emb|CAD15658.1| (AL646067) PROBABLE GLUTAMATE RACEMASE PROTEIN [Ralstonia
           solanacearum]
          Length = 277

 Score =  237 bits (605), Expect = 5e-62
 Identities = 130/250 (52%), Positives = 169/250 (67%), Gaps = 1/250 (0%)








 
Structural Features:
COG0796, MurI, Glutamate racemase [Cell envelope biogenesis, outer membrane]
CD-Length = 269 residues,  96.7% aligned
Score =  309 bits (794), Expect = 2e-85
Identities = 131/261 (50%), Positives = 173/261 (66%), Gaps = 3/261 (1%)

pfam01177, Asp_Glu_race, Asp/Glu/Hydantoin racemase.
CD-Length = 212 residues,  99.5% aligned
Score =  202 bits (515), Expect = 4e-53
Identities = 105/215 (48%), Positives = 133/215 (61%), Gaps = 4/215 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6191




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23333




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36551




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3094_cdd.html


    		




 
URL:

















    ReferenceID:36597




  
Author/Investigator(s):
        Liu L, Yoshimura T, Endo K, Esaki N, Soda K.





  
Title:
        Cloning and expression of the glutamate racemase gene of Bacillus pumilus.




  
PubMed:
        9354391


MedLine:
        




    
Source:
J Biochem (Tokyo). 1997 Jun;121(6):1155-61.






    
Reference Type: 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9354391

















    ReferenceID:36598




  
Author/Investigator(s):
        Malathi KC, Wachi M, Nagai K.





  
Title:
        Isolation of the murI gene from Brevibacterium lactofermentum ATCC 13869 encoding D-glutamate racemase.




  
PubMed:
        10386367


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 Jun 15;175(2):193-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10386367

















    ReferenceID:36599




  
Author/Investigator(s):
        Doublet P, van Heijenoort J, Bohin JP, Mengin-Lecreulx D.





  
Title:
        The murI gene of Escherichia coli is an essential gene that encodes a glutamate racemase activity.




  
PubMed:
        8098327


MedLine:
        




    
Source:
J Bacteriol. 1993 May;175(10):2970-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8098327









           
Homologs By Global Alignment 

           

       



     
Gene ID:3094
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4985
                           PA4662_tr
                           translation of PA4662
                           265
                           99.62
                           99.62
                           0
                           1315.0
     

     
     
140568
                           gb|AAG08049.1|AE004880_5
                           (AE004880) glutamate racemase [Pseudomonas aeruginosa PAO1]
                           265
                           99.62
                           99.62
                           0
                           1315.0
     

     
     
140569
                           gb|AAN66361.1|AE016264_5
                           (AE016776) glutamate racemase [Pseudomonas putida KT2440]
                           265
                           67.54
                           78.49
                           0
                           914.0
     

     
     
140570
                           gb|AAO54641.1|
                           (AE016859) glutamate racemase [Pseudomonas syringae pv. tomato str. DC3000]
                           275
                           62.18
                           72.72
                           10
                           840.0
     

     
     
140571
                           emb|CAD15658.1|
                           (AL646067) PROBABLE GLUTAMATE RACEMASE PROTEIN [Ralstonia solanacearum]
                           278
                           47.12
                           62.58
                           14
                           602.0
     

     






      

          
GC ORFID: 46096How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4662Glimmer Score: 

          
Start: 4766534Stop: 4765737

          
Length: 798


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4662 translation of PA4662Homolog Bit Score 511.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4299 (ORF: BLASTX 328.0), t_PA4662 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGGTTGAGTCGGCCGCGGTCGGCGTTTTCGATTCCGGGGTCGGCGGGCTCAGCGTATTGCGTGAGATT 
CGTGCGCGCCTGCCATCCGAATCGTTGCTCTACGTCGCCGACAACGCCCATGTGCCCTACGGCGAGAAGAGC 
GCGGAGTACATCCGCGAACGCTGCGAGCGGATCGGTGATTTCCTTCTGGAGCAGGGCGCCAAGGCTCTGGTA 
CTGGCCTGCAATACCGCGACGGCAGCGGCTGCGGCGGAATTGCGCGAACGTTATCCGCAGGTGCAGCTGGTG 
GCCATGGAGCCGGCGGTGAAGCCGGCCGCGGCGGCCACCCGCAACGGTCGGGTCGGCGTCCTCGCCACCACC 
GGAACTCTGAAGAGCGCGCGTTTCGCCGCCTTGCTCGACCGCTTCGCCAGCGATGTACAGGTCTTCACCCAG 
CCCTGTCCGGGGCTGGTCGAGCGCATCGAAGCCGGCGATCTCTATGGGCCGCAAACCCGGGCACTGCTGGAA 
CGCCTGCTGGCACCCATCCTCGAACAGGGCTGCGATACGCTGATCCTCGGCTGTACTCATTATCCCTTCGTC 
AAACCCCTGCTGGCCGAGCTGATCCCGGCGGAGATGGCGGTGATCGATACGGGAGCGGCTGTCGCCCGGCAG 
CTGGAGCGCGTATTGTCGGCGCGGGCGCTACTGGCCTCTGGCCAGGCCGCGACGCCGCGTTTCTGGACCAGC 
GCGCTGCCGGAGGAGATGGAAAGGATCCTGCCGATCCTCTGGGGAAGCCCGGAAAGCGTAGGAAAATTAGTC 
GTTTAG