ViewGene
Locus Name:
GeneID:
Location:

GeneID:3085
TrivialName: PA14_08440
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_08440
PAO1 Ortholog Locus:PA0658
Sequence Length:801
Protein Length:266
Start: 723004
Stop: 722204
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04047.1|AE004500_13 (AE004500) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 264/266 (99%), Positives = 264/266 (99%)
Sequence:
ATGCCCCGTTATGCCCTGATCACCGGCGCTTCCAGCGGTATCGGCCTGGCCCTAGCCGAAGCCCTGGCCCGG
CGCGGTCGCGCCCTGATCCTGGTGGCGCGCCAGCGCGACGCGCTGGAGAGCATCGCCTATGAGCTGGCGCAG
CGTTTCGGCGTGGAAGTGCTGTTCCGCACCTGCGATCTTTCCGAGCCGCTGCAACTGTCAGGGTTGTTGCAC
GAACTGGAACAGGGCGAACGACAGATCGACCTGCTGGTTAACAATGCCGGGATCGGTACCTCCGGCCCCTTC
ATCGACCAGGAATGGGCGCGGGAACAGGAGCTGATCGAACTCAACATCCTGGCCCTGGCCCGCCTTTGCCAC
TCCCTCGGCGCCCGCATGGCGGCGCAGGGCGGTGGCCAGATACTCAATATCGCCTCGGTGGCCGCCTTCCAG
CCCGGCGCCTGGATGAGCAACTACTACGCCAGCAAGGCCTACGTCCTGCACTTCTCGGAAGGCTTGCGGGAA
GAACTGAAAGGGCGCGGCGTCAAGGTCTCGGTGCTGTGCCCTGGGCCAACCCGTACGGCATTCTTCCGGGAA
GCCGGGATGCGGCTCGGCAGGCTCGACCGGAGCAAGCTGATGATGACCCCGGAGGAAGTCGCGCTGCTGGCC
CTGCGCGGGCTGGACAAGAACCGCGCCCTGATCGTCCCCGGTTGGCGCAATCGCCTGCTGGCGCTGAGCCCA
CGCCTGGCGCCACGCTGGCTGGTGCGCCGACTCGCCGCGCGGATCAACGCCTCGGCGGTACCCTCCGTCCCA
CGGGGTTGA
Translation:
MPRYALITGASSGIGLALAEALARRGRALILVARQRDALESIAYELAQRFGVEVLFRTCDLSEPLQLSGLLH
ELEQGERQIDLLVNNAGIGTSGPFIDQEWAREQELIELNILALARLCHSLGARMAAQGGGQILNIASVAAFQ
PGAWMSNYYASKAYVLHFSEGLREELKGRGVKVSVLCPGPTRTAFFREAGMRLGRLDRSKLMMTPEEVALLA
LRGLDKNRALIVPGWRNRLLALSPRLAPRWLVRRLAARINASAVPSVPRG*
AnnotationID:3093GeneID:3085
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 18:06:33
Gene Name:
Confidence Code:3
GeneProduct:putative short chain alcohol dehydrogenase
Cell Localization:()
Synonyms:PA0658
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0300,COG4221,pfam00106
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54143.1| (AE016858) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 258

 Score =  358 bits (918), Expect = 2e-98
 Identities = 186/261 (71%), Positives = 213/261 (81%), Gaps = 4/261 (1%)

>gb|AAN66059.1|AE016234_12 (AE016775) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas putida KT2440]
          Length = 260

 Score =  338 bits (866), Expect = 2e-92
 Identities = 174/262 (66%), Positives = 206/262 (78%), Gaps = 5/262 (1%)








 
Structural Features:
COG0300, DltE, Short-chain dehydrogenases of various substrate specificities
CD-Length = 265 residues,  97.4% aligned
Score =  301 bits (773), Expect = 5e-83
Identities = 132/258 (51%), Positives = 170/258 (65%)

COG4221, Short-chain alcohol dehydrogenase of unknown specificity 
CD-Length = 246 residues,  87.4% aligned
Score =  157 bits (399), Expect = 1e-39
Identities = 71/218 (32%), Positives = 112/218 (51%), Gaps = 8/218 (3%)

pfam00106, adh_short, short chain dehydrogenase
CD-Length = 247 residues,  96.8% aligned
Score =  153 bits (387), Expect = 3e-38
Identities = 73/240 (30%), Positives = 116/240 (48%), Gaps = 14/240 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6173




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23315




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34595




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3085_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3085
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4970
                           PA0658_tr
                           translation of PA0658
                           266
                           99.24
                           99.24
                           0
                           1322.0
     

     
     
140331
                           gb|AAG04047.1|AE004500_13
                           (AE004500) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           266
                           99.24
                           99.24
                           0
                           1322.0
     

     
     
140332
                           gb|AAO54143.1|
                           (AE016858) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas syringae pv. tomato str. DC3000]
                           266
                           69.92
                           80.07
                           8
                           922.0
     

     
     
140333
                           gb|AAN66059.1|AE016234_12
                           (AE016775) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas putida KT2440]
                           268
                           65.29
                           77.23
                           10
                           865.0
     

     
     
140334
                           dbj|BAB75452.1|
                           (AP003594) ORF_ID:all3753~probable short-chain dehydrogenase [Nostoc sp. PCC 7120]
                           270
                           39.62
                           55.92
                           11
                           486.0
     

     






      

          
GC ORFID: 46087How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0658Glimmer Score: 

          
Start: 6524347Stop: 6523547

          
Length: 801


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0658 translation of PA0658Homolog Bit Score 513.0

          
Other Homologs:  psyr_15may02_Scaffold14_revised_gene2398 (ORF: BLASTX 361.0), t_PA0658 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCCGTTATGCCCTGATCACCGGCGCTTCCAGCGGTATCGGCCTGGCCCTAGCCGAAGCCCTGGCCCGG 
CGCGGTCGCGCCCTGATCCTGGTGGCGCGCCAGCGCGACGCGCTGGAGAGCATCGCCTATGAGCTGGCGCAG 
CGTTTCGGCGTGGAAGTGCTGTTCCGCACCTGCGATCTTTCCGAGCCGCTGCAACTGTCAGGGTTGTTGCAC 
GAACTGGAACAGGGCGAACGACAGATCGACCTGCTGGTTAACAATGCCGGGATCGGTACCTCCGGCCCCTTC 
ATCGACCAGGAATGGGCGCGGGAACAGGAGCTGATCGAACTCAACATCCTGGCCCTGGCCCGCCTTTGCCAC 
TCCCTCGGCGCCCGCATGGCGGCGCAGGGCGGTGGCCAGATACTCAATATCGCCTCGGTGGCCGCCTTCCAG 
CCCGGCGCCTGGATGAGCAACTACTACGCCAGCAAGGCCTACGTCCTGCACTTCTCGGAAGGCTTGCGGGAA 
GAACTGAAAGGGCGCGGCGTCAAGGTCTCGGTGCTGTGCCCTGGGCCAACCCGTACGGCATTCTTCCGGGAA 
GCCGGGATGCGGCTCGGCAGGCTCGACCGGAGCAAGCTGATGATGACCCCGGAGGAAGTCGCGCTGCTGGCC 
CTGCGCGGGCTGGACAAGAACCGCGCCCTGATCGTCCCCGGTTGGCGCAATCGCCTGCTGGCGCTGAGCCCA 
CGCCTGGCGCCACGCTGGCTGGTGCGCCGACTCGCCGCGCGGATCAACGCCTCGGCGGTACCCTCCGTCCCA 
CGGGGTTGA