ViewGene
Locus Name:
GeneID:
Location:

GeneID:3084
TrivialName: PA14_05150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05150
PAO1 Ortholog Locus:PA0393
Sequence Length:822
Protein Length:273
Start: 450387
Stop: 449566
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03782.1|AE004476_12 (AE004476) pyrroline-5-carboxylate reductase [Pseudomonas aeruginosa PAO1]
Identities = 272/273 (99%), Positives = 272/273 (99%)
Sequence:
ATGAGCACACCCCGCATAGCGTTCATCGGCGCCGGCAACATGGCCGCCAGCCTGATCGGCGGCCTGCGCGCC
CAAGGCGTGCCGGCGGCGCAGATCCGCGCCAGCGACCCGGGCGCCGAGCAACGCGCGAAGATCGCCGGCGAG
TTCGCCATCGACGTGGTCGAGTCCAACGCCGAGGCCGTGGCCGACGCCGACGTCGTGGTCCTGTCGGTCAAG
CCGCAGGCCATGAAAGCCGTGTGCCAGGCCTTGGCGCCGGCGCTGAAGCCGGAGCAACTGATCGTCTCCATC
GCCGCCGGCATCCCCTGCGCCAGCCTCGAAGCCTGGCTCGGCCAGCCGCGCCCGGTGGTCCGCTGCATGCCC
AACACCCCGGCGCTGCTGCGCCAGGGCGCCAGCGGGCTGTACGCCAACGCGCAGGTCAGCGCCGCGCAACGC
GAGCAGGCCGGGCAGCTGCTGTCTGCGGTAGGCATCGCCCTGTGGCTGGACGACGAAGCGCAGATCGACGCG
GTGACCGCGGTGTCGGGCAGCGGCCCGGCGTATTTCTTCCTGCTGATGCAGGCCATGACCGACGCCGGCGAG
AAGCTCGGCCTGTCCCGCGAGACAGCCTCGCGCCTGACCCTGCAGACCGCCCTCGGCGCGGCGCAGATGGCG
CTGTCCAGCGAGGTCGAGCCGGCCGAACTGCGGCGCCGGGTGACTTCGCCCAACGGCACCACCGAGGCGGCG
ATCAAATCCTTCCAGGCCAACGGCTTCGAAGCACTAGTGGAGCAGGCCCTGAACGCCGCCAGCCAGCGCTCC
GCCGAGCTGGCCGAACAGCTTGGCCAATAA
Translation:
MSTPRIAFIGAGNMAASLIGGLRAQGVPAAQIRASDPGAEQRAKIAGEFAIDVVESNAEAVADADVVVLSVK
PQAMKAVCQALAPALKPEQLIVSIAAGIPCASLEAWLGQPRPVVRCMPNTPALLRQGASGLYANAQVSAAQR
EQAGQLLSAVGIALWLDDEAQIDAVTAVSGSGPAYFFLLMQAMTDAGEKLGLSRETASRLTLQTALGAAQMA
LSSEVEPAELRRRVTSPNGTTEAAIKSFQANGFEALVEQALNAASQRSAELAEQLGQ*
AnnotationID:3092GeneID:3084
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 07:07:02
Gene Name:proC
Confidence Code:1
GeneProduct:pyrroline-5-carboxylate reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0345,pfam01089
Secondary Functional Category(ies):
EC Number:1.5.1.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70660.1|AE016710_5 (AE016793) pyrroline-5-carboxylate reductase [Pseudomonas putida
           KT2440]
          Length = 272

 Score =  392 bits (1007), Expect = e-108
 Identities = 208/273 (76%), Positives = 236/273 (86%), Gaps = 1/273 (0%)

>gb|AAO58475.1| (AE016874) pyrroline-5-carboxylate reductase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 272

 Score =  358 bits (918), Expect = 2e-98
 Identities = 192/273 (70%), Positives = 220/273 (80%), Gaps = 1/273 (0%)

>gb|AAM37771.1| (AE011934) pyrroline-5-carboxylate reductase [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 284

 Score =  275 bits (704), Expect = 2e-73
 Identities = 152/268 (56%), Positives = 183/268 (68%), Gaps = 1/268 (0%)






 
Structural Features:
COG0345, ProC, Pyrroline-5-carboxylate reductase 
[Amino acid transport and metabolism]
CD-Length = 266 residues,  99.2% aligned
Score =  260 bits (665), Expect = 1e-70
Identities = 124/267 (46%), Positives = 171/267 (64%), Gaps = 4/267 (1%)

pfam01089, P5CR, Delta 1-pyrroline-5-carboxylate reductase.
CD-Length = 248 residues,  99.6% aligned
Score =  249 bits (637), Expect = 3e-67
Identities = 118/249 (47%), Positives = 155/249 (62%), Gaps = 3/249 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6171




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23313




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33728




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3084_cdd.html


    		




 
URL:

















    ReferenceID:35383




  
Author/Investigator(s):
        Savioz A, Jeenes DJ, Kocher HP, Haas D.





  
Title:
        Comparison of proC and other housekeeping genes of Pseudomonas aeruginosa with their counterparts in Escherichia coli.




  
PubMed:
        2107123


MedLine:
        




    
Source:
Gene. 1990 Jan 31;86(1):107-11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2107123

















    ReferenceID:35384




  
Author/Investigator(s):
        Savioz A, Zimmermann A, Haas D.





  
Title:
        Pseudomonas aeruginosa promoters which contain a conserved GG-N10-GC motif but appear to be RpoN-independent.




  
PubMed:
        8479442


MedLine:
        




    
Source:
Mol Gen Genet. 1993 Apr;238(1-2):74-80.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8479442









           
Homologs By Global Alignment 

           

       



     
Gene ID:3084
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4969
                           PA0393_tr
                           translation of PA0393
                           273
                           99.63
                           99.63
                           0
                           1321.0
     

     
     
140292
                           gb|AAG03782.1|AE004476_12
                           (AE004476) pyrroline-5-carboxylate reductase [Pseudomonas aeruginosa PAO1]
                           273
                           99.63
                           99.63
                           0
                           1321.0
     

     
     
140293
                           gb|AAA25975.1|
                           (M33557) delta-1-pyrroline-5-carboxylate reductase (EC 1.5.1.2) [Pseudomonas aeruginosa]
                           273
                           99.63
                           99.63
                           0
                           1321.0
     

     
     
140294
                           gb|AAN70660.1|AE016710_5
                           (AE016793) pyrroline-5-carboxylate reductase [Pseudomonas putida KT2440]
                           273
                           76.19
                           86.44
                           1
                           1009.0
     

     
     
140295
                           gb|AAO58475.1|
                           (AE016874) pyrroline-5-carboxylate reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           273
                           70.32
                           80.58
                           1
                           920.0
     

     






      

          
GC ORFID: 46086How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0393Glimmer Score: 

          
Start: 6251729Stop: 6250908

          
Length: 822


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0393 translation of PA0393Homolog Bit Score 513.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3719 (ORF: BLASTX 354.0), t_PA0393 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCACACCCCGCATAGCGTTCATCGGCGCCGGCAACATGGCCGCCAGCCTGATCGGCGGCCTGCGCGCC 
CAAGGCGTGCCGGCGGCGCAGATCCGCGCCAGCGACCCGGGCGCCGAGCAACGCGCGAAGATCGCCGGCGAG 
TTCGCCATCGACGTGGTCGAGTCCAACGCCGAGGCCGTGGCCGACGCCGACGTCGTGGTCCTGTCGGTCAAG 
CCGCAGGCCATGAAAGCCGTGTGCCAGGCCTTGGCGCCGGCGCTGAAGCCGGAGCAACTGATCGTCTCCATC 
GCCGCCGGCATCCCCTGCGCCAGCCTCGAAGCCTGGCTCGGCCAGCCGCGCCCGGTGGTCCGCTGCATGCCC 
AACACCCCGGCGCTGCTGCGCCAGGGCGCCAGCGGGCTGTACGCCAACGCGCAGGTCAGCGCCGCGCAACGC 
GAGCAGGCCGGGCAGCTGCTGTCTGCGGTAGGCATCGCCCTGTGGCTGGACGACGAAGCGCAGATCGACGCG 
GTGACCGCGGTGTCGGGCAGCGGCCCGGCGTATTTCTTCCTGCTGATGCAGGCCATGACCGACGCCGGCGAG 
AAGCTCGGCCTGTCCCGCGAGACAGCCTCGCGCCTGACCCTGCAGACCGCCCTCGGCGCGGCGCAGATGGCG 
CTGTCCAGCGAGGTCGAGCCGGCCGAACTGCGGCGCCGGGTGACTTCGCCCAACGGCACCACCGAGGCGGCG 
ATCAAATCCTTCCAGGCCAACGGCTTCGAAGCACTAGTGGAGCAGGCCCTGAACGCCGCCAGCCAGCGCTCC 
GCCGAGCTGGCCGAACAGCTTGGCCAATAA