ViewGene
Locus Name:
GeneID:
Location:

GeneID:3083
TrivialName: PA14_02770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02770
PAO1 Ortholog Locus:PA0227
Sequence Length:783
Protein Length:260
Start: 246480
Stop: 247262
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03616.1|AE004460_9 (AE004460) probable CoA transferase, subunit B [Pseudomonas aeruginosa PAO1]
Identities = 260/260 (100%), Positives = 260/260 (100%)
Sequence:
ATGAGCAGCTACAGCACCAACGAGATGATGACCGTGGCCGCCGCCCGCCGCCTGCACAACGGTGCGGTGTGC
TTCGTCGGCATCGGCCTGCCGTCGAAGGCCGCCAACCTGGCGCGCCTGACTTCCTCGCCGGACGTCGTGCTG
ATCTACGAGTCCGGCCCGATCGGCGCCAAGCCCAGCGTCCTGCCACTGTCCATCGGCGACGGCGAGTTGGCC
GAGACCGCCGATACCGTGGTCCCCACCGGTGAGATATTCCGCTACTGGCTGCAGGGCGGACGGATCGACGTC
GGCTTCCTCGGCGCGGCCCAGGTCGACCGCTTCGGCAACATCAACACCACCGTGATCGGCGACTACCGCCAG
CCCAAGGTGCGCCTGCCGGGAGCCGGCGGCGCGCCGGAAATCGCCGGCAGCGCCAAGGAAGTGCTGATCATC
CTCAAGCAGTCGCACCGCACCTTCGTCGACAAGCTGGCCTTCGTCACCTCGGTCGGCCATGGCGAGGGCGGC
GACTCGCGCCAGCGCCTGGGCCTGCCGGGCAAGGGGCCGGTGGCGATCATCACCGACCTGTGCATCATGGAG
CCGGAAGCCGGCAGCAACGAGTTCGTGGTCACTTCGCTGCATCCGGGCGTGACCCGCGAGCAGGTGGTCGAG
GCCACCGGTTGGGCGATCCGCTTCGCCGAGCGGGTCGCCGAGACGCCCGCGCCGAGCGAGGTCGAACTCGCC
GCGCTGCGCGACCTCGAGGCGCGCACCGCGGCCGCCCATGGACAGAAGGGGAGCGACGAATGA
Translation:
MSSYSTNEMMTVAAARRLHNGAVCFVGIGLPSKAANLARLTSSPDVVLIYESGPIGAKPSVLPLSIGDGELA
ETADTVVPTGEIFRYWLQGGRIDVGFLGAAQVDRFGNINTTVIGDYRQPKVRLPGAGGAPEIAGSAKEVLII
LKQSHRTFVDKLAFVTSVGHGEGGDSRQRLGLPGKGPVAIITDLCIMEPEAGSNEFVVTSLHPGVTREQVVE
ATGWAIRFAERVAETPAPSEVELAALRDLEARTAAAHGQKGSDE*
AnnotationID:3091GeneID:3083
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 13:01:35
Gene Name:
Confidence Code:3
GeneProduct:putative CoA transferase, subunit B
Cell Localization:()
Synonyms:pcaJ
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2057,pfam01144
Secondary Functional Category(ies):fatty acid and phospholipid metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL02406.1| (AY044272) 3-oxoadipate:succinyl-CoA transferase subunit B
           [Pseudomonas sp. B13]
          Length = 260

 Score =  478 bits (1230), Expect = e-134
 Identities = 239/260 (91%), Positives = 251/260 (96%)

>gb|AAO57759.1| (AE016871) 3-oxoadipate:succinyl-CoA transferase, subunit B
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 259

 Score =  437 bits (1124), Expect = e-122
 Identities = 218/253 (86%), Positives = 235/253 (92%)

>gb|AAM39684.1| (AE012133) glutaconate CoA transferase subunit B [Xanthomonas
           campestris pv. campestris str. ATCC 33913]
          Length = 262

 Score =  379 bits (973), Expect = e-105
 Identities = 187/258 (72%), Positives = 214/258 (82%)








 
Structural Features:
COG2057, AtoA, Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit 
[Lipid metabolism]
CD-Length = 225 residues,  99.6% aligned
Score =  222 bits (567), Expect = 4e-59
Identities = 73/241 (30%), Positives = 108/241 (44%), Gaps = 22/241 (9%)

pfam01144, CoA_trans, Coenzyme A transferase.
CD-Length = 218 residues, 100.0% aligned
Score =  147 bits (372), Expect = 1e-36
Identities = 63/227 (27%), Positives = 85/227 (37%), Gaps = 24/227 (10%)







   
Genomic Context:






   
Comment:
other possible gene name: catJ
other possible gene product: 
3-oxoadipate:succinyl-CoA transferase subunit B

another possible gene name: gctB
another possible gene product: 
glutaconate CoA transferase subunit B


















    ReferenceID:6169




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23311




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33587




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3083_cdd.html


    		




 
URL:

















    ReferenceID:35456




  
Author/Investigator(s):
        Parales RE, Harwood CS.





  
Title:
        Characterization of the genes encoding beta-ketoadipate: succinyl-coenzyme A transferase in Pseudomonas putida.




  
PubMed:
        1624453


MedLine:
        




    
Source:
J Bacteriol. 1992 Jul;174(14):4657-66.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1624453

















    ReferenceID:35457




  
Author/Investigator(s):
        Gobel M, Kassel-Cati K, Schmidt E, Reineke W.





  
Title:
        Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase.




  
PubMed:
        11741863


MedLine:
        




    
Source:
J Bacteriol. 2002 Jan;184(1):216-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11741863

















    ReferenceID:35458




  
Author/Investigator(s):
        Mack M, Bendrat K, Zelder O, Eckel E, Linder D, Buckel W.





  
Title:
        Location of the two genes encoding glutaconate coenzyme A-transferase at the beginning of the hydroxyglutarate operon in Acidaminococcus fermentans.




  
PubMed:
        7957258


MedLine:
        




    
Source:
Eur J Biochem. 1994 Nov 15;226(1):41-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7957258









           
Homologs By Global Alignment 

           

       



     
Gene ID:3083
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4968
                           PA0227_tr
                           translation of PA0227
                           260
                           99.99
                           99.99
                           0
                           1321.0
     

     
     
140284
                           gb|AAG03616.1|AE004460_9
                           (AE004460) probable CoA transferase, subunit B [Pseudomonas aeruginosa PAO1]
                           260
                           99.99
                           99.99
                           0
                           1321.0
     

     
     
140285
                           gb|AAL02406.1|
                           (AY044272) 3-oxoadipate:succinyl-CoA transferase subunit B [Pseudomonas sp. B13]
                           260
                           91.92
                           96.53
                           0
                           1230.0
     

     
     
140286
                           gb|AAO57759.1|
                           (AE016871) 3-oxoadipate:succinyl-CoA transferase, subunit B [Pseudomonas syringae pv. tomato str. DC3000]
                           260
                           83.84
                           90.76
                           1
                           1122.0
     

     
     
140287
                           gb|AAM39684.1|
                           (AE012133) glutaconate CoA transferase subunit B [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           262
                           71.37
                           81.67
                           2
                           971.0
     

     






      

          
GC ORFID: 46085How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0227Glimmer Score: 

          
Start: 6047821Stop: 6048603

          
Length: 783


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0227 translation of PA0227Homolog Bit Score 513.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6192 (ORF: BLASTX 441.0), t_PA0227 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAGCTACAGCACCAACGAGATGATGACCGTGGCCGCCGCCCGCCGCCTGCACAACGGTGCGGTGTGC 
TTCGTCGGCATCGGCCTGCCGTCGAAGGCCGCCAACCTGGCGCGCCTGACTTCCTCGCCGGACGTCGTGCTG 
ATCTACGAGTCCGGCCCGATCGGCGCCAAGCCCAGCGTCCTGCCACTGTCCATCGGCGACGGCGAGTTGGCC 
GAGACCGCCGATACCGTGGTCCCCACCGGTGAGATATTCCGCTACTGGCTGCAGGGCGGACGGATCGACGTC 
GGCTTCCTCGGCGCGGCCCAGGTCGACCGCTTCGGCAACATCAACACCACCGTGATCGGCGACTACCGCCAG 
CCCAAGGTGCGCCTGCCGGGAGCCGGCGGCGCGCCGGAAATCGCCGGCAGCGCCAAGGAAGTGCTGATCATC 
CTCAAGCAGTCGCACCGCACCTTCGTCGACAAGCTGGCCTTCGTCACCTCGGTCGGCCATGGCGAGGGCGGC 
GACTCGCGCCAGCGCCTGGGCCTGCCGGGCAAGGGGCCGGTGGCGATCATCACCGACCTGTGCATCATGGAG 
CCGGAAGCCGGCAGCAACGAGTTCGTGGTCACTTCGCTGCATCCGGGCGTGACCCGCGAGCAGGTGGTCGAG 
GCCACCGGTTGGGCGATCCGCTTCGCCGAGCGGGTCGCCGAGACGCCCGCGCCGAGCGAGGTCGAACTCGCC 
GCGCTGCGCGACCTCGAGGCGCGCACCGCGGCCGCCCATGGACAGAAGGGGAGCGACGAATGA