ViewGene
Locus Name:
GeneID:
Location:

GeneID:3065
TrivialName: PA14_32410
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32410
PAO1 Ortholog Locus:
Sequence Length:915
Protein Length:304
Start: 2819753
Stop: 2818839
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: emb|CAA07694.1| (AJ007825) MexT protein [Pseudomonas aeruginosa]
Identities = 303/304 (99%), Positives = 304/304 (100%)
Sequence:
ATGAACCGAAACGACCTGCGCCGCGTCGATCTGAACCTGCTGATCGTGTTCGAGACCCTGATGCACGAACGC
AGCGTGACCCGCGCCGCAGAGAAACTGTTCCTCGGCCAGCCGGCCATCAGCGCCGCGCTGTCGCGCCTGCGC
ACGCTGTTCGACGACCCGCTGTTCGTCCGTACCGGACGCAGCATGGAGCCCACCGCGCGAGCCCAGGAAATC
TTCGCCCACCTGTCGCCGGCGCTGGATTCCATCTCCACCGCCATGAGTCGCGCCAGCGAGTTCGATCCGGCG
ACCAGCACCGCGGTGTTCCGCATCGGCCTTTCCGACGACGTCGAGTTCGGCCTGCTGCCGCCCCTGCTCCGC
CGCCTGCGCGCGGAGGCGCCGGGGATCGTCCTCGTCGTGCGCCGCGCCAACTATCTATTGATGCCGAACCTG
CTGGCCTCGGGGGAGATATCGGTGGGCGTCAGCTACACCGACGAACTGCCGGCCAACGCCAAGCGCAAGACC
GTGCGCCGCAGCAAGCCGAAGATCCTCCGCGCCGACTCCGCGCCCGGCCAGCTGACCCTCGACGACTACTGC
GCGCGACCGCACGCGCTGGTGTCCTTCGCCGGCGACCTCAGCGGCTTCGTCGACGAGGAACTGGAGAAATTC
GGCCGCAAGCGCAAGGTGGTCCTGGCGGTGCCGCAGTTCAACGGCCTCGGCACCCTCCTCGCCGGCACCGAC
ATCATCGCCACCGTGCCCGACTACGCCGCCCAGGCGCTGATCGCCGCCGGCGGCCTGCGCGCCGAGGACCCG
CCGTTCGAGACCCGCGCCTTCGAACTGTCGATGGCCTGGCGCGGCGCCCAGGACAACGACCCGGCGGAACGC
TGGCTGCGCTCGCGGATCAGCATGTTCATCGGCGATCCGGACAGTCTCTGA
Translation:
MNRNDLRRVDLNLLIVFETLMHERSVTRAAEKLFLGQPAISAALSRLRTLFDDPLFVRTGRSMEPTARAQEI
FAHLSPALDSISTAMSRASEFDPATSTAVFRIGLSDDVEFGLLPPLLRRLRAEAPGIVLVVRRANYLLMPNL
LASGEISVGVSYTDELPANAKRKTVRRSKPKILRADSAPGQLTLDDYCARPHALVSFAGDLSGFVDEELEKF
GRKRKVVLAVPQFNGLGTLLAGTDIIATVPDYAAQALIAAGGLRAEDPPFETRAFELSMAWRGAQDNDPAER
WLRSRISMFIGDPDSL*
AnnotationID:3073GeneID:3065
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 14:02:44
Gene Name:mexT
Confidence Code:1
GeneProduct:transcriptional regulator MexT
Cell Localization:()
Synonyms:ttgS
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:putative LysR family transcriptional regulator
Functional Category Confidence Code:5
COGs:COG0583
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|84326817|ref|ZP_00974841.1|  COG0583: Transcriptional regulator [Pseudomonas aeruginosa 2192]
 gi|84320865|ref|ZP_00969233.1|  COG0583: Transcriptional regulator [Pseudomonas aeruginosa C3719]
Length=304

 Score =  591 bits (1523),  Expect = 1e-167
 Identities = 304/304 (100%), Positives = 304/304 (100%), Gaps = 0/304 (0%)

gi|3646369|emb|CAA07694.1|  MexT protein [Pseudomonas aeruginosa]
Length=304

 Score =  590 bits (1520),  Expect = 3e-167
 Identities = 303/304 (99%), Positives = 304/304 (100%), Gaps = 0/304 (0%)






 
Structural Features:
COG0583, LysR, Transcriptional regulator [Transcription]
CD-Length = 297 residues,  96.3% aligned
Score =  115 bits (289), Expect = 5e-27
Identities = 75/288 (26%), Positives = 114/288 (39%), Gaps = 5/288 (1%)







   
Genomic Context:






   
Comment:
100% identical to mexT of strains 2192 and C3719.  99% (303/304) identical to MexT from PAO1 as published in 
PMID 10515918 (NCBI Accession CAA07694).  However, PAO1 genome annotation for ths gene (PA2492) is 
longer (347 aa instead of 304 aa).


















    ReferenceID:6133




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23275




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33033




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3065_cdd.html


    		




 
URL:

















    ReferenceID:34495




  
Author/Investigator(s):
        Kohler T, Epp SF, Curty LK, Pechere JC.





  
Title:
        Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa.




  
PubMed:
        10515918


MedLine:
        




    
Source:
J Bacteriol. 1999 Oct;181(20):6300-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10515918

















    ReferenceID:34496




  
Author/Investigator(s):
        Maseda H, Saito K, Nakajima A, Nakae T.





  
Title:
        Variation of the mexT gene, a regulator of the MexEF-oprN efflux pump expression in wild-type strains of Pseudomonas aeruginosa.




  
PubMed:
        11040437


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2000 Nov 1;192(1):107-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11040437









           
Homologs By Global Alignment 

           

       



     
Gene ID:3065
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
139760
                           emb|CAA07694.1|
                           (AJ007825) MexT protein [Pseudomonas aeruginosa]
                           304
                           99.67
                           99.99
                           0
                           1520.0
     

     
     
139765
                           gb|AAO56530.1|
                           (AE016866) transcriptional regulator, LysR family [Pseudomonas syringae pv. tomato str. DC3000]
                           304
                           86.51
                           91.44
                           0
                           1324.0
     

     
     
139762
                           emb|CAD59442.1|
                           (Y19106) putative LysR family transcriptional regulator [Pseudomonas putida]
                           304
                           86.51
                           91.11
                           0
                           1331.0
     

     
     
139763
                           gb|AAN68434.1|AE016476_3
                           (AE016784) transcriptional regulator MexT [Pseudomonas putida KT2440]
                           304
                           86.51
                           90.78
                           0
                           1329.0
     

     
     
139764
                           emb|CAB91105.1|
                           (AJ276408) TtgS protein [Pseudomonas putida]
                           304
                           86.18
                           90.78
                           0
                           1325.0
     

     






      

          
GC ORFID: 46067How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold7_revised_gene7138Glimmer Score: 

          
Start: 2134171Stop: 2135085

          
Length: 915


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: psyr_15may02_Scaffold7_revised_gene7138 related to: gi|7801208|emb|CAB91105.1| (AJ276408) TtgS protein [Pseudomonas putida]Homolog Bit Score 516.0

          
Other Homologs:  t_PA2492 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACCGAAACGACCTGCGCCGCGTCGATCTGAACCTGCTGATCGTGTTCGAGACCCTGATGCACGAACGC 
AGCGTGACCCGCGCCGCAGAGAAACTGTTCCTCGGCCAGCCGGCCATCAGCGCCGCGCTGTCGCGCCTGCGC 
ACGCTGTTCGACGACCCGCTGTTCGTCCGTACCGGACGCAGCATGGAGCCCACCGCGCGAGCCCAGGAAATC 
TTCGCCCACCTGTCGCCGGCGCTGGATTCCATCTCCACCGCCATGAGTCGCGCCAGCGAGTTCGATCCGGCG 
ACCAGCACCGCGGTGTTCCGCATCGGCCTTTCCGACGACGTCGAGTTCGGCCTGCTGCCGCCCCTGCTCCGC 
CGCCTGCGCGCGGAGGCGCCGGGGATCGTCCTCGTCGTGCGCCGCGCCAACTATCTATTGATGCCGAACCTG 
CTGGCCTCGGGGGAGATATCGGTGGGCGTCAGCTACACCGACGAACTGCCGGCCAACGCCAAGCGCAAGACC 
GTGCGCCGCAGCAAGCCGAAGATCCTCCGCGCCGACTCCGCGCCCGGCCAGCTGACCCTCGACGACTACTGC 
GCGCGACCGCACGCGCTGGTGTCCTTCGCCGGCGACCTCAGCGGCTTCGTCGACGAGGAACTGGAGAAATTC 
GGCCGCAAGCGCAAGGTGGTCCTGGCGGTGCCGCAGTTCAACGGCCTCGGCACCCTCCTCGCCGGCACCGAC 
ATCATCGCCACCGTGCCCGACTACGCCGCCCAGGCGCTGATCGCCGCCGGCGGCCTGCGCGCCGAGGACCCG 
CCGTTCGAGACCCGCGCCTTCGAACTGTCGATGGCCTGGCGCGGCGCCCAGGACAACGACCCGGCGGAACGC 
TGGCTGCGCTCGCGGATCAGCATGTTCATCGGCGATCCGGACAGTCTCTGA