ViewGene
Locus Name:
GeneID:
Location:

GeneID:3057
TrivialName: PA14_04980
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04980
PAO1 Ortholog Locus:PA0381
Sequence Length:798
Protein Length:265
Start: 438455
Stop: 439252
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03770.1|AE004475_12 (AE004475) thiamine biosynthesis protein, thiazole moiety [Pseudomonas aeruginosa PAO1]
Identities = 265/265 (100%), Positives = 265/265 (100%)
Sequence:
ATGAGCCAAGCATCCAGTACCGATACCCCCTTCGTCATCGCCGGCCGTACCTATGGCTCGCGCCTGCTGGTG
GGGACCGGCAAGTACAAGGACCTCGACGAGACCCGCCGGGCCATCGAGGCCTCCGGAGCGGAGATCGTCACG
GTGGCGGTGCGGCGCACCAACATCGGGCAGAATCCCGACGAGCCGAACCTACTGGATGTGATCCCGCCGGAT
CGCTACACCATCCTGCCGAACACCGCCGGCTGCTATGACGCGGTCGAGGCCGTGCGCACCTGCCGCCTGGCC
CGCGAGCTGCTGGACGGGCACAACCTGGTCAAGCTGGAAGTCCTCGCCGACCAGAAGACTCTGTTCCCCAAC
GTCGTCGAGACCCTCAAGGCCGCCGAGCAACTGGTGAAGGACGGCTTCGACGTGATGGTGTACACCAGCGAC
GACCCGATCATCGCCCGCCAGCTGGCCGAGATCGGCTGCATCGCGGTGATGCCGCTGGCCGGCCTGATCGGC
TCCGGCCTGGGCATCTGCAACCCCTACAACCTGCGCATCATCCTCGAGGAAGCCAAGGTCCCGGTGCTGGTC
GACGCCGGCGTGGGCACCGCTTCCGATGCAGCGATCGCCATGGAGCTGGGTTGCGAGGCGGTGCTGATGAAC
ACCGCCATCGCCCATGCCAAGGACCCGGTGATGATGGCCGAGGCCATGAAGCACGCGATCGTCGCCGGTCGC
CTCGCCTACCTGGCCGGACGCATGCCGCGCAAGCTGTATGCCAGCGCCTCGTCGCCGCTGGACGGCCTGATC
GACTGA
Translation:
MSQASSTDTPFVIAGRTYGSRLLVGTGKYKDLDETRRAIEASGAEIVTVAVRRTNIGQNPDEPNLLDVIPPD
RYTILPNTAGCYDAVEAVRTCRLARELLDGHNLVKLEVLADQKTLFPNVVETLKAAEQLVKDGFDVMVYTSD
DPIIARQLAEIGCIAVMPLAGLIGSGLGICNPYNLRIILEEAKVPVLVDAGVGTASDAAIAMELGCEAVLMN
TAIAHAKDPVMMAEAMKHAIVAGRLAYLAGRMPRKLYASASSPLDGLID*
AnnotationID:3065GeneID:3057
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 06:06:07
Gene Name:thiG
Confidence Code:2
GeneProduct:thiamine biosynthesis protein, thiazole moiety
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam05690,COG2022
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53978.1| (AE016857) thiazole biosynthesis protein ThiG [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 264

 Score =  462 bits (1188), Expect = e-129
 Identities = 231/258 (89%), Positives = 248/258 (96%)

>gb|AAN70669.1|AE016710_14 (AE016793) thiG protein, putative [Pseudomonas putida KT2440]
          Length = 270

 Score =  441 bits (1135), Expect = e-123
 Identities = 223/264 (84%), Positives = 245/264 (92%), Gaps = 6/264 (2%)

>gb|AAM42420.1| (AE012430) thiamine biosynthesis protein [Xanthomonas campestris
           pv. campestris str. ATCC 33913]
          Length = 264

 Score =  388 bits (996), Expect = e-107
 Identities = 193/260 (74%), Positives = 227/260 (87%)








 
Structural Features:
pfam05690, ThiG, Thiazole biosynthesis protein ThiG.
CD-Length = 255 residues, 100.0% aligned
Score =  383 bits (985), Expect = 1e-107
Identities = 165/256 (64%), Positives = 193/256 (75%), Gaps = 1/256 (0%)

COG2022, ThiG, Uncharacterized enzyme of thiazole biosynthesis 
[Nucleotide transport and metabolism]
CD-Length = 262 residues,  98.9% aligned
Score =  376 bits (968), Expect = 1e-105
Identities = 164/261 (62%), Positives = 196/261 (75%), Gaps = 2/261 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6117




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23259




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33725




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3057_cdd.html


    		




 
URL:

















    ReferenceID:35390




  
Author/Investigator(s):
        Kelleher NL, Taylor SV, Grannis D, Kinsland C, Chiu HJ, Begley TP, McLafferty FW.





  
Title:
        Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry.




  
PubMed:
        10082377


MedLine:
        




    
Source:
Protein Sci. 1998 Aug;7(8):1796-801.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10082377

















    ReferenceID:35391




  
Author/Investigator(s):
        Miranda-Rios J, Morera C, Taboada H, Davalos A, Encarnacion S, Mora J, Soberon M.





  
Title:
        Expression of thiamin biosynthetic genes (thiCOGE) and production of symbiotic terminal oxidase cbb3 in Rhizobium etli.




  
PubMed:
        9371431


MedLine:
        




    
Source:
J Bacteriol. 1997 Nov;179(22):6887-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9371431

















    ReferenceID:35392




  
Author/Investigator(s):
        Vander Horn PB, Backstrom AD, Stewart V, Begley TP.





  
Title:
        Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.




  
PubMed:
        8432721


MedLine:
        




    
Source:
J Bacteriol. 1993 Feb;175(4):982-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8432721









           
Homologs By Global Alignment 

           

       



     
Gene ID:3057
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4935
                           PA0381_tr
                           translation of PA0381
                           265
                           99.99
                           99.99
                           0
                           1332.0
     

     
     
139438
                           gb|AAG03770.1|AE004475_12
                           (AE004475) thiamine biosynthesis protein, thiazole moiety [Pseudomonas aeruginosa PAO1]
                           265
                           99.99
                           99.99
                           0
                           1332.0
     

     
     
139439
                           gb|AAO53978.1|
                           (AE016857) thiazole biosynthesis protein ThiG [Pseudomonas syringae pv. tomato str. DC3000]
                           265
                           88.30
                           94.33
                           1
                           1188.0
     

     
     
139440
                           gb|AAN70669.1|AE016710_14
                           (AE016793) thiG protein, putative [Pseudomonas putida KT2440]
                           271
                           83.39
                           91.14
                           7
                           1139.0
     

     
     
139444
                           emb|CAD58331.1|
                           (AL844858) Thiamine biosynthesis protein [Azoarcus sp. EbN1]
                           267
                           73.40
                           82.77
                           8
                           982.0
     

     






      

          
GC ORFID: 46059How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0381Glimmer Score: 

          
Start: 6239797Stop: 6240594

          
Length: 798


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0381 translation of PA0381Homolog Bit Score 517.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6618 (ORF: BLASTX 460.0), t_PA0381 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAAGCATCCAGTACCGATACCCCCTTCGTCATCGCCGGCCGTACCTATGGCTCGCGCCTGCTGGTG 
GGGACCGGCAAGTACAAGGACCTCGACGAGACCCGCCGGGCCATCGAGGCCTCCGGAGCGGAGATCGTCACG 
GTGGCGGTGCGGCGCACCAACATCGGGCAGAATCCCGACGAGCCGAACCTACTGGATGTGATCCCGCCGGAT 
CGCTACACCATCCTGCCGAACACCGCCGGCTGCTATGACGCGGTCGAGGCCGTGCGCACCTGCCGCCTGGCC 
CGCGAGCTGCTGGACGGGCACAACCTGGTCAAGCTGGAAGTCCTCGCCGACCAGAAGACTCTGTTCCCCAAC 
GTCGTCGAGACCCTCAAGGCCGCCGAGCAACTGGTGAAGGACGGCTTCGACGTGATGGTGTACACCAGCGAC 
GACCCGATCATCGCCCGCCAGCTGGCCGAGATCGGCTGCATCGCGGTGATGCCGCTGGCCGGCCTGATCGGC 
TCCGGCCTGGGCATCTGCAACCCCTACAACCTGCGCATCATCCTCGAGGAAGCCAAGGTCCCGGTGCTGGTC 
GACGCCGGCGTGGGCACCGCTTCCGATGCAGCGATCGCCATGGAGCTGGGTTGCGAGGCGGTGCTGATGAAC 
ACCGCCATCGCCCATGCCAAGGACCCGGTGATGATGGCCGAGGCCATGAAGCACGCGATCGTCGCCGGTCGC 
CTCGCCTACCTGGCCGGACGCATGCCGCGCAAGCTGTATGCCAGCGCCTCGTCGCCGCTGGACGGCCTGATC 
GACTGA