ViewGene
Locus Name:
GeneID:
Location:

GeneID:3054
TrivialName: PA14_26240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_26240
PAO1 Ortholog Locus:PA2923
Sequence Length:786
Protein Length:261
Start: 2290717
Stop: 2289932
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06311.1|AE004718_8 (AE004718) periplasmic histidine-binding protein HisJ [Pseudomonas aeruginosa PAO1]
Identities = 260/261 (99%), Positives = 260/261 (99%)
Sequence:
ATGACAAGAACCCTCGGCAGCATCGCCCTCTCCCTCCTCCTCGCCGCACCGGCCGGCGCCGCCGACTGGACC
GGCAAGGTCCTGCGCCTGGGCGTCGACCCGACCTACCCGCCGCTCGAATACAAGCAGCCGGACGGCCGCCTG
GCAGGCTTCGGCATCGAGATCGGCGAAGCCCTCTGCGCGGAGCTGCGAGCCCGCTGCGAATGGGTCGAAAGC
AACTGGGACGGCATCATCCCGGCGCTGCTGTCGCGCAAGATCGACGCCATCGTCTCGTCGATGACCATCACG
CCAAAACGCGCACAGCACATCGCCTTCACCGACCGCGTCTCCAACGCGCCGAGCCGCCTGGTGGTACGCCGC
GGCTCGGCGCTCCTGCCCGACGCCGAGAGCCTGCGCGGCAAGCGCGTGGGGGTCGGCCAGGGCTCCAACCAG
GAGGCCTACGCCAAGGCCGAATGGGCCGCCAAGGGGGTGCGGGTCGTCTCCTACCAGAACCAGGACCAGGTC
TACGCCGACCTCGTCATGGGCCGCCTCGACGCCTCGCTGCAAGGTGCGGTGCAGGCCAGCTACGGCTTCCTC
GACAAGCCGGTCGGCAAGGATTTCGAACTGGCCGGAGCGGTGCTGGACGACCCTCGCTACTTCGGGGTCGGC
GACGGCATCGGCCTGCGCAAGGAAGATGTGGCGCTGCGCGAGGACCTGAACAAGGCGCTGGCGACGATCCGC
GCCAACGGGACCTACGCGCGAATCAACGCCCGTTACTTCGATTTCGACCTCTACGGCGCGCCCTGA
Translation:
MTRTLGSIALSLLLAAPAGAADWTGKVLRLGVDPTYPPLEYKQPDGRLAGFGIEIGEALCAELRARCEWVES
NWDGIIPALLSRKIDAIVSSMTITPKRAQHIAFTDRVSNAPSRLVVRRGSALLPDAESLRGKRVGVGQGSNQ
EAYAKAEWAAKGVRVVSYQNQDQVYADLVMGRLDASLQGAVQASYGFLDKPVGKDFELAGAVLDDPRYFGVG
DGIGLRKEDVALREDLNKALATIRANGTYARINARYFDFDLYGAP*
AnnotationID:3062GeneID:3054
AnnotatorUID: diggins
Modification Date/Time: 2005-04-08 09:09:20
Gene Name:hisJ
Confidence Code:2
GeneProduct:periplasmic histidine-binding protein HisJ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00497,smart00062,cd00134,COG0834
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM85176.1|AE013763_10 (AE013763) histidine-binding periplasmic protein of high-affinity
           histidine transport system [Yersinia pestis KIM]
          Length = 351

 Score =  261 bits (668), Expect = 2e-69
 Identities = 130/259 (50%), Positives = 178/259 (68%)

>gb|AAP17716.1| (AE016986) histidine-binding periplasmic protein of high-affinity
           histidine transport system [Shigella flexneri 2a str.
           2457T]
          Length = 260

 Score =  261 bits (667), Expect = 3e-69
 Identities = 129/259 (49%), Positives = 176/259 (67%)

>gb|AAG57438.1|AE005463_1 (AE005463) histidine-binding periplasmic protein of high-affinity
           histidine transport system [Escherichia coli O157:H7
           EDL933]
          Length = 260

 Score =  261 bits (666), Expect = 4e-69
 Identities = 129/254 (50%), Positives = 176/254 (69%), Gaps = 2/254 (0%)








 
Structural Features:
pfam00497, SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3.
CD-Length = 224 residues, 100.0% aligned
Score =  212 bits (542), Expect = 3e-56
Identities = 89/229 (38%), Positives = 136/229 (59%), Gaps = 6/229 (2%)

cd00134, PBPb, Bacterial periplasmic transport system
CD-Length = 218 residues, 100.0% aligned
Score =  160 bits (407), Expect = 1e-40
Identities = 73/226 (32%), Positives = 126/226 (55%), Gaps = 8/226 (3%)

smart00062, PBPb, Bacterial periplasmic substrate-binding proteins
CD-Length = 222 residues, 100.0% aligned
Score =  162 bits (411), Expect = 4e-41
Identities = 75/230 (32%), Positives = 122/230 (53%), Gaps = 8/230 (3%)

COG0834, HisJ, ABC-type amino acid transport/signal transduction systems, 
periplasmic component/domain 
[Amino acid transport and metabolism / Signal transduction mechanisms]
CD-Length = 275 residues,  97.1% aligned
Score =  155 bits (392), Expect = 4e-39
Identities = 84/270 (31%), Positives = 136/270 (50%), Gaps = 12/270 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6111




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23253




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36142




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3054_cdd.html


    		




 
URL:

















    ReferenceID:36416




  
Author/Investigator(s):
        Higgins CF, Haag PD, Nikaido K, Ardeshir F, Garcia G, Ames GF.





  
Title:
        Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium.




  
PubMed:
        7050725


MedLine:
        




    
Source:
Nature. 1982 Aug 19;298(5876):723-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7050725









           
Homologs By Global Alignment 

           

       



     
Gene ID:3054
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4929
                           PA2923_tr
                           translation of PA2923
                           261
                           99.61
                           99.61
                           0
                           1331.0
     

     
     
139349
                           gb|AAG06311.1|AE004718_8
                           (AE004718) periplasmic histidine-binding protein HisJ [Pseudomonas aeruginosa PAO1]
                           261
                           99.61
                           99.61
                           0
                           1331.0
     

     
     
139350
                           gb|AAQ58527.1|
                           (AE016912) subunit of the lysine/arginine/ornithine transporter (ABC superfamily) [Chromobacterium violaceum ATCC 12472]
                           262
                           51.14
                           69.08
                           4
                           683.0
     

     
     
139358
                           dbj|BAA16155.1|
                           (D90862) HISTIDINE-BINDING PERIPLASMIC PROTEIN PRECURSOR (HBP). [Escherichia coli]
                           263
                           49.42
                           68.06
                           5
                           664.0
     

     
     
139357
                           dbj|BAB36616.1|
                           (AP002561) histidine transport system histidine-binding periplasmic protein [Escherichia coli O157:H7]
                           263
                           49.42
                           68.06
                           5
                           664.0
     

     






      

          
GC ORFID: 46056How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2923Glimmer Score: 

          
Start: 2663208Stop: 2663993

          
Length: 786


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2923 translation of PA2923Homolog Bit Score 517.0

          
Other Homologs:  t_PA0888 (ORF: BLASTX 231.0), t_PA3865 (ORF: BLASTX 230.0), psyr_15may02_Scaffold8_revised_gene7808 (ORF: BLASTX 220.0), psyr_15may02_Scaffold5_revised_gene6099 (ORF: BLASTX 209.0), t_PA2923 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACAAGAACCCTCGGCAGCATCGCCCTCTCCCTCCTCCTCGCCGCACCGGCCGGCGCCGCCGACTGGACC 
GGCAAGGTCCTGCGCCTGGGCGTCGACCCGACCTACCCGCCGCTCGAATACAAGCAGCCGGACGGCCGCCTG 
GCAGGCTTCGGCATCGAGATCGGCGAAGCCCTCTGCGCGGAGCTGCGAGCCCGCTGCGAATGGGTCGAAAGC 
AACTGGGACGGCATCATCCCGGCGCTGCTGTCGCGCAAGATCGACGCCATCGTCTCGTCGATGACCATCACG 
CCAAAACGCGCACAGCACATCGCCTTCACCGACCGCGTCTCCAACGCGCCGAGCCGCCTGGTGGTACGCCGC 
GGCTCGGCGCTCCTGCCCGACGCCGAGAGCCTGCGCGGCAAGCGCGTGGGGGTCGGCCAGGGCTCCAACCAG 
GAGGCCTACGCCAAGGCCGAATGGGCCGCCAAGGGGGTGCGGGTCGTCTCCTACCAGAACCAGGACCAGGTC 
TACGCCGACCTCGTCATGGGCCGCCTCGACGCCTCGCTGCAAGGTGCGGTGCAGGCCAGCTACGGCTTCCTC 
GACAAGCCGGTCGGCAAGGATTTCGAACTGGCCGGAGCGGTGCTGGACGACCCTCGCTACTTCGGGGTCGGC 
GACGGCATCGGCCTGCGCAAGGAAGATGTGGCGCTGCGCGAGGACCTGAACAAGGCGCTGGCGACGATCCGC 
GCCAACGGGACCTACGCGCGAATCAACGCCCGTTACTTCGATTTCGACCTCTACGGCGCGCCCTGA