ViewGene
Locus Name:
GeneID:
Location:

GeneID:305
TrivialName: PA14_21880
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_21880
PAO1 Ortholog Locus:PA3257
Sequence Length:2097
Protein Length:698
Start: 1904259
Stop: 1902163
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06645.1|AE004748_3 (AE004748) periplasmic tail-specific protease [Pseudomonas aeruginosa PAO1]
Identities = 698/698 (100%), Positives = 698/698 (100%)
Sequence:
ATGAAGCGTTTTCTGCCCCGTACCGCCCTGTTGCTCCTCCTCGGCGCCAGTTCGCTGCCGCTGTTCGCCTCG
ACCGCCGTCTCCGTCGGCAGCGCCCAGAATTCCTGGGACAGCCTGCAGCCGGACCGCGAGCAGGTCATCGCC
AGCCTCAACGTAGTGGAGCTGCTCAAGCGGCACCACTACAACAAGCCGCCGCTGAACGATGAACGCTCGGCC
AAGATCTACGACAGCTACCTGAAGATGCTCGATCCGGCGCGGATGTACTTCACCGCCGGCGACATCGCCGAG
TTCGATCGCTGGCGCAACAGGTTCGACGACTTCCTCAAGAGCGGCGATCTCGATCCCGGCTTCGTCATCTAC
AAGCGTCACCTCGACCGCCTGAAAGGCCGTCTCGACTACGCCCTGGCGATGCTCGACAAGGGCGTCGACAAG
ATCGACTTCAGCGTCGACGAGAGCCTGCTGATCGACCGCGAGAAGGCCCCCTGGGCGAAGGACACCGCAGAG
CTCGACGACCTCTGGCGCAAGAAGGTGAAGGACGAGGTCCTGCGCCTGAAGATCACCGGCAAGGACGACAAG
GCGATCCAGGAACAGCTCACCAAGCGCTACAAGAACCAGCTGTCGCGCCTGAAGCAGACCCGCAGCGAAGAC
ATCTTCCAGGCCTACATCAACGCCTTCGCCCAGTCCTACGATCCGCACACCCAGTACCTGTCGCCGGACAAC
GCGGAAAACTTCGACATCAACATGAGCCTCTCGCTGGAAGGCATCGGCGCGGTGCTGCAGAGCGACAACGAC
TACGTCAAGGTCGTACGCCTGGTGCCGGCCGGCCCGGCGGCGAAGAGCAAGCAGATCGCCACCTCCGACAAG
ATCATCGGCGTCGCCCAGGGCAAGGGCGAAATGGTCGACGTGGTCGGCTGGCGCCTGGATGAAGTGGTCAAG
CTGATCCGCGGGCCGAAAGGCTCCCAGGTACGCCTGGAAGTGATTCCGGCGAGCAACGCGCCGAACGACCAG
ACCAGCAAGATCGTCGCCATCACCCGCGAGGCGGTGAAGCTGGAGGACCAGGCGGCGAAGAAATCCATCATC
AAGGTCGACCACGAAGGCCGCAGCTACAAGCTCGGCGTGATCGACCTGCCGGCCTTCTACCTGGACTTCAAG
GCCTACCGCGCCGGCGACCCGAACTACAAGAGCACCACCCGCGACGTGAAGAAGCTGGTCGCCGAATTGCAG
AAGGACAAGGTCGACGGCATCGTCCTCGACCTGCGCAACAACGGCGGCGGCTCCCTGCAGGAAGCCACCGAG
CTGACCGGCCTGTTCATCGACCAGGGCCCGACCGTACTGGTGCGCAACAGCGACGGCCGCGTCGACGTGCTC
AACGACGACGAAGGCAAGGCCTTCTATACCGGCCCGCTGGCGGTGCTGGTCAACCGTCTCTCGGCCTCCGCC
TCGGAGATCTTCGCCGGCGCCATGCAGGACTACCACCGCGCGCTGATCCTCGGTGGACAGACCTTCGGCAAG
GGCACCGTGCAGACCATCCAGCCGCTCAACCACGGCGAACTGAAGCTGACCCTGGCGAAGTTCTACCGTGTC
TCCGGGCAGAGCACCCAGCACCAGGGCGTGATCCCGGACATCAGCTACCCGTCCATCGTCGACGACAAGGAA
ATCGGCGAAAGCGCCCTGCCCGACTCGATGCCCTGGGACACCATCCGCCCGGTGGTGAAGGACGAGGCCAAC
CCGTTCAAGCCGTTCCTCGCGCAACTCAAGGCCCAGCACGACGCGCGCACCGAGAACAACGCCGACTTCGTC
TACGCCCGCGAGCGCCTGGCGCTGGCCCAGGAGCTGATGAAGGAGAAGACCGTCAGCCTGAACGAGGCCAAG
CGCCGCGCCCAGCAGACCAGCATCGAGAACCGCCAGTTGGCCATGGAGAACGCCCTGCGCAAGTCCAAGGGC
CAGGAGCCGCTGAAGGAACTCAAGCGCGAGGACGAGAACGCCCTGCCGGAAGAGGACGACAAGACCAAGCCG
CAGGACGACGCCTACCTCACCGAGTCCGGGCACATCCTGCTGGACTACCTGAACCTCAACTCGGCGGTGGCC
AAGCACTGA
Translation:
MKRFLPRTALLLLLGASSLPLFASTAVSVGSAQNSWDSLQPDREQVIASLNVVELLKRHHYNKPPLNDERSA
KIYDSYLKMLDPARMYFTAGDIAEFDRWRNRFDDFLKSGDLDPGFVIYKRHLDRLKGRLDYALAMLDKGVDK
IDFSVDESLLIDREKAPWAKDTAELDDLWRKKVKDEVLRLKITGKDDKAIQEQLTKRYKNQLSRLKQTRSED
IFQAYINAFAQSYDPHTQYLSPDNAENFDINMSLSLEGIGAVLQSDNDYVKVVRLVPAGPAAKSKQIATSDK
IIGVAQGKGEMVDVVGWRLDEVVKLIRGPKGSQVRLEVIPASNAPNDQTSKIVAITREAVKLEDQAAKKSII
KVDHEGRSYKLGVIDLPAFYLDFKAYRAGDPNYKSTTRDVKKLVAELQKDKVDGIVLDLRNNGGGSLQEATE
LTGLFIDQGPTVLVRNSDGRVDVLNDDEGKAFYTGPLAVLVNRLSASASEIFAGAMQDYHRALILGGQTFGK
GTVQTIQPLNHGELKLTLAKFYRVSGQSTQHQGVIPDISYPSIVDDKEIGESALPDSMPWDTIRPVVKDEAN
PFKPFLAQLKAQHDARTENNADFVYARERLALAQELMKEKTVSLNEAKRRAQQTSIENRQLAMENALRKSKG
QEPLKELKREDENALPEEDDKTKPQDDAYLTESGHILLDYLNLNSAVAKH*
AnnotationID:315GeneID:305
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 14:02:40
Gene Name:tsp
Confidence Code:2
GeneProduct:periplasmic tail-specific protease
Cell Localization:()
Synonyms:prc
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:smart00245,COG0793
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:3.4.21.102
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67340.1|AE016360_6 (AE016780) tail-specific protease prc [Pseudomonas putida KT2440]
          Length = 704

 Score = 1098 bits (2841), Expect = 0.0
 Identities = 541/697 (77%), Positives = 622/697 (89%), Gaps = 5/697 (0%)

>gb|AAO57351.1| (AE016870) tail-specific protease [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 693

 Score = 1080 bits (2794), Expect = 0.0
 Identities = 530/697 (76%), Positives = 617/697 (88%), Gaps = 5/697 (0%)






 
Structural Features:
smart00245, TSPc, tail specific protease; tail specific protease
CD-Length = 193 residues,  97.9% aligned
Score =  160 bits (407), Expect = 4e-40
Identities = 79/207 (38%), Positives = 111/207 (53%), Gaps = 19/207 (9%)

COG0793, Prc, Periplasmic protease 
[Cell envelope biogenesis, outer membrane]
CD-Length = 406 residues,  96.6% aligned
Score =  328 bits (841), Expect = 2e-90
Identities = 166/436 (38%), Positives = 227/436 (52%), Gaps = 47/436 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:613




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17755




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32286




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:305_cdd.html


    		




 
URL:

















    ReferenceID:33988




  
Author/Investigator(s):
        Hara H, Yamamoto Y, Higashitani A, Suzuki H, Nishimura Y.





  
Title:
        Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3.




  
PubMed:
        1856173


MedLine:
        




    
Source:
J Bacteriol. 1991 Aug;173(15):4799-813.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1856173









           
Homologs By Global Alignment 

           

       



     
Gene ID:305
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
497
                           PA3257_tr
                           translation of PA3257
                           698
                           99.99
                           99.99
                           0
                           3552.0
     

     
     
25187
                           gb|AAG06645.1|AE004748_3
                           (AE004748) periplasmic tail-specific protease [Pseudomonas aeruginosa PAO1]
                           698
                           99.99
                           99.99
                           0
                           3552.0
     

     
     
25188
                           gb|AAN67340.1|AE016360_6
                           (AE016780) tail-specific protease prc [Pseudomonas putida KT2440]
                           709
                           76.30
                           87.72
                           16
                           2844.0
     

     
     
25189
                           gb|AAO57351.1|
                           (AE016870) tail-specific protease [Pseudomonas syringae pv. tomato str. DC3000]
                           698
                           75.93
                           88.39
                           5
                           2797.0
     

     
     
25191
                           gb|AAO10991.1|AE016805_248
                           (AE016805) Periplasmic protease [Vibrio vulnificus CMCP6]
                           710
                           42.53
                           59.71
                           56
                           1395.0
     

     






      

          
GC ORFID: 43307How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3257Glimmer Score: 

          
Start: 3049672Stop: 3051768

          
Length: 2097


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3257 translation of PA3257Homolog Bit Score 1372.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2682 (ORF: BLASTX 1079.0), t_PA3257 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCGTTTTCTGCCCCGTACCGCCCTGTTGCTCCTCCTCGGCGCCAGTTCGCTGCCGCTGTTCGCCTCG 
ACCGCCGTCTCCGTCGGCAGCGCCCAGAATTCCTGGGACAGCCTGCAGCCGGACCGCGAGCAGGTCATCGCC 
AGCCTCAACGTAGTGGAGCTGCTCAAGCGGCACCACTACAACAAGCCGCCGCTGAACGATGAACGCTCGGCC 
AAGATCTACGACAGCTACCTGAAGATGCTCGATCCGGCGCGGATGTACTTCACCGCCGGCGACATCGCCGAG 
TTCGATCGCTGGCGCAACAGGTTCGACGACTTCCTCAAGAGCGGCGATCTCGATCCCGGCTTCGTCATCTAC 
AAGCGTCACCTCGACCGCCTGAAAGGCCGTCTCGACTACGCCCTGGCGATGCTCGACAAGGGCGTCGACAAG 
ATCGACTTCAGCGTCGACGAGAGCCTGCTGATCGACCGCGAGAAGGCCCCCTGGGCGAAGGACACCGCAGAG 
CTCGACGACCTCTGGCGCAAGAAGGTGAAGGACGAGGTCCTGCGCCTGAAGATCACCGGCAAGGACGACAAG 
GCGATCCAGGAACAGCTCACCAAGCGCTACAAGAACCAGCTGTCGCGCCTGAAGCAGACCCGCAGCGAAGAC 
ATCTTCCAGGCCTACATCAACGCCTTCGCCCAGTCCTACGATCCGCACACCCAGTACCTGTCGCCGGACAAC 
GCGGAAAACTTCGACATCAACATGAGCCTCTCGCTGGAAGGCATCGGCGCGGTGCTGCAGAGCGACAACGAC 
TACGTCAAGGTCGTACGCCTGGTGCCGGCCGGCCCGGCGGCGAAGAGCAAGCAGATCGCCACCTCCGACAAG 
ATCATCGGCGTCGCCCAGGGCAAGGGCGAAATGGTCGACGTGGTCGGCTGGCGCCTGGATGAAGTGGTCAAG 
CTGATCCGCGGGCCGAAAGGCTCCCAGGTACGCCTGGAAGTGATTCCGGCGAGCAACGCGCCGAACGACCAG 
ACCAGCAAGATCGTCGCCATCACCCGCGAGGCGGTGAAGCTGGAGGACCAGGCGGCGAAGAAATCCATCATC 
AAGGTCGACCACGAAGGCCGCAGCTACAAGCTCGGCGTGATCGACCTGCCGGCCTTCTACCTGGACTTCAAG 
GCCTACCGCGCCGGCGACCCGAACTACAAGAGCACCACCCGCGACGTGAAGAAGCTGGTCGCCGAATTGCAG 
AAGGACAAGGTCGACGGCATCGTCCTCGACCTGCGCAACAACGGCGGCGGCTCCCTGCAGGAAGCCACCGAG 
CTGACCGGCCTGTTCATCGACCAGGGCCCGACCGTACTGGTGCGCAACAGCGACGGCCGCGTCGACGTGCTC 
AACGACGACGAAGGCAAGGCCTTCTATACCGGCCCGCTGGCGGTGCTGGTCAACCGTCTCTCGGCCTCCGCC 
TCGGAGATCTTCGCCGGCGCCATGCAGGACTACCACCGCGCGCTGATCCTCGGTGGACAGACCTTCGGCAAG 
GGCACCGTGCAGACCATCCAGCCGCTCAACCACGGCGAACTGAAGCTGACCCTGGCGAAGTTCTACCGTGTC 
TCCGGGCAGAGCACCCAGCACCAGGGCGTGATCCCGGACATCAGCTACCCGTCCATCGTCGACGACAAGGAA 
ATCGGCGAAAGCGCCCTGCCCGACTCGATGCCCTGGGACACCATCCGCCCGGTGGTGAAGGACGAGGCCAAC 
CCGTTCAAGCCGTTCCTCGCGCAACTCAAGGCCCAGCACGACGCGCGCACCGAGAACAACGCCGACTTCGTC 
TACGCCCGCGAGCGCCTGGCGCTGGCCCAGGAGCTGATGAAGGAGAAGACCGTCAGCCTGAACGAGGCCAAG 
CGCCGCGCCCAGCAGACCAGCATCGAGAACCGCCAGTTGGCCATGGAGAACGCCCTGCGCAAGTCCAAGGGC 
CAGGAGCCGCTGAAGGAACTCAAGCGCGAGGACGAGAACGCCCTGCCGGAAGAGGACGACAAGACCAAGCCG 
CAGGACGACGCCTACCTCACCGAGTCCGGGCACATCCTGCTGGACTACCTGAACCTCAACTCGGCGGTGGCC 
AAGCACTGA