ViewGene
Locus Name:
GeneID:
Location:

GeneID:3044
TrivialName: PA14_19700
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19700
PAO1 Ortholog Locus:PA3430
Sequence Length:780
Protein Length:259
Start: 1699716
Stop: 1700495
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06818.1|AE004764_4 (AE004764) probable aldolase [Pseudomonas aeruginosa PAO1]
Identities = 258/259 (99%), Positives = 258/259 (99%)
Sequence:
GTGACCGCCGTTCACTCCCTGCCCGCCGATGTGCAAGCCCAGGTCTCCGCCGCCGAATGGCAGACCCGCGTC
GATCTCGCTGCCTGCTACCGGCTGGTCGCCCTGCACGGTTGGGACGACCTGATCTTCACCCACATCTCGGCC
AAGGTGCCGGGCACCGAGGACTTCCTGATCAACCCGTTCGGCCTGATGTTCCACGAGATCACCGCCTCCAGC
CTGGTGAAGGTGGACGCCAGCGGCAAGAAGCTGATGGACAGCCCCTACGAGATCAACCCGGCCGGCTACACC
ATCCACAGCGCGGTGCACGAGGTCCGCCATGACGTCGAATGCGTGCTGCACACCCATACCGCCGCGGGGATC
GCGGTGTCCTGCCAGAAGCAGGGCCTGTTGCCGCTGTCGCAGCAATCGTTGTTCGTGCTCTCCAGCCTGTCC
TACCACGCCTACGAGGGCGTCGCCCTGAACCATGAGGAAAAGGCCCGCCTGCAGGCTGACCTGGGGACCAGC
AACTTCCTGATCCTGCCCAACCACGGCCTGCTCACCTGCGGCGGTTCCATCGCCGATACCTTCCTCATGATG
TTCACTCTGCAACGCGCCTGCGAGGTACAGGTGATGGCGCAGAGCGGCGGCGCCGAGCTGATCCACATCCCG
GGGCAGATCCTCGCCGGCGCGCGCGACATGATCGCCGGGGTGATGCGCAGCAAGACCGGCATGGGCGGCCAG
CTCGCCTGGCCGGCGCTGCTGCGCAAGCTCGACCAGCAGAACCCGGGCTACCGCCAGTGA
Translation:
MTAVHSLPADVQAQVSAAEWQTRVDLAACYRLVALHGWDDLIFTHISAKVPGTEDFLINPFGLMFHEITASS
LVKVDASGKKLMDSPYEINPAGYTIHSAVHEVRHDVECVLHTHTAAGIAVSCQKQGLLPLSQQSLFVLSSLS
YHAYEGVALNHEEKARLQADLGTSNFLILPNHGLLTCGGSIADTFLMMFTLQRACEVQVMAQSGGAELIHIP
GQILAGARDMIAGVMRSKTGMGGQLAWPALLRKLDQQNPGYRQ*
AnnotationID:3052GeneID:3044
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 17:05:00
Gene Name:
Confidence Code:3
GeneProduct:putative class II aldolase/adducin domain protein
Cell Localization:()
Synonyms:PA3430
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00398,pfam00596,COG0235
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK23184.1| (AE005797) class II aldolase/adducin domain protein [Caulobacter
           crescentus CB15]
          Length = 300

 Score =  272 bits (696), Expect = 1e-72
 Identities = 140/248 (56%), Positives = 176/248 (70%), Gaps = 4/248 (1%)

>gb|AAO55994.1| (AE016864) class II aldolase/adducin domain protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 271

 Score =  232 bits (591), Expect = 2e-60
 Identities = 127/253 (50%), Positives = 167/253 (66%), Gaps = 6/253 (2%)

>gb|AAL17922.1| (AY054120) putative aldolase [Mycobacterium smegmatis]
          Length = 255

 Score =  227 bits (579), Expect = 5e-59
 Identities = 121/250 (48%), Positives = 161/250 (64%), Gaps = 3/250 (1%)








 
Structural Features:
cd00398, Aldolase_II, Class II Aldolase and Adducin head (N-terminal) domain.
CD-Length = 209 residues,  91.9% aligned
Score =  112 bits (282), Expect = 4e-26
Identities = 63/197 (31%), Positives = 95/197 (48%), Gaps = 10/197 (5%)

pfam00596, Aldolase_II, Class II Aldolase and Adducin N-terminal domain. 
CD-Length = 198 residues,  95.5% aligned
Score =  166 bits (421), Expect = 3e-42
Identities = 65/191 (34%), Positives = 104/191 (54%), Gaps = 6/191 (3%)

COG0235, AraD, Ribulose-5-phosphate 4-epimerase and related 
epimerases and aldolases 
[Carbohydrate transport and metabolism]
CD-Length = 219 residues,  92.2% aligned
Score =  158 bits (402), Expect = 4e-40
Identities = 60/204 (29%), Positives = 94/204 (46%), Gaps = 4/204 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14731




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23233




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32137




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3044_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3044
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4915
                           PA3430_tr
                           translation of PA3430
                           259
                           99.61
                           99.61
                           0
                           1336.0
     

     
     
139104
                           gb|AAG06818.1|AE004764_4
                           (AE004764) probable aldolase [Pseudomonas aeruginosa PAO1]
                           259
                           99.22
                           99.61
                           0
                           1333.0
     

     
     
139106
                           dbj|BAC48330.1|
                           (AP005946) hypothetical aldolase class II protein [Bradyrhizobium japonicum USDA 110]
                           260
                           52.30
                           68.84
                           2
                           688.0
     

     
     
139114
                           gb|AAN68831.1|AE016516_1
                           (AE016786) class II aldolase/adducin domain protein [Pseudomonas putida KT2440]
                           271
                           49.07
                           63.83
                           19
                           593.0
     

     
     
139108
                           gb|AAR05337.1|
                           (AY372455) putative aldolase class II protein [uncultured marine alpha proteobacterium HOT2C01]
                           260
                           48.07
                           62.30
                           13
                           615.0
     

     






      

          
GC ORFID: 46046How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3430Glimmer Score: 

          
Start: 3254216Stop: 3253437

          
Length: 780


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3430 translation of PA3430Homolog Bit Score 519.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1258 (ORF: BLASTX 229.0), t_PA3430 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGACCGCCGTTCACTCCCTGCCCGCCGATGTGCAAGCCCAGGTCTCCGCCGCCGAATGGCAGACCCGCGTC 
GATCTCGCTGCCTGCTACCGGCTGGTCGCCCTGCACGGTTGGGACGACCTGATCTTCACCCACATCTCGGCC 
AAGGTGCCGGGCACCGAGGACTTCCTGATCAACCCGTTCGGCCTGATGTTCCACGAGATCACCGCCTCCAGC 
CTGGTGAAGGTGGACGCCAGCGGCAAGAAGCTGATGGACAGCCCCTACGAGATCAACCCGGCCGGCTACACC 
ATCCACAGCGCGGTGCACGAGGTCCGCCATGACGTCGAATGCGTGCTGCACACCCATACCGCCGCGGGGATC 
GCGGTGTCCTGCCAGAAGCAGGGCCTGTTGCCGCTGTCGCAGCAATCGTTGTTCGTGCTCTCCAGCCTGTCC 
TACCACGCCTACGAGGGCGTCGCCCTGAACCATGAGGAAAAGGCCCGCCTGCAGGCTGACCTGGGGACCAGC 
AACTTCCTGATCCTGCCCAACCACGGCCTGCTCACCTGCGGCGGTTCCATCGCCGATACCTTCCTCATGATG 
TTCACTCTGCAACGCGCCTGCGAGGTACAGGTGATGGCGCAGAGCGGCGGCGCCGAGCTGATCCACATCCCG 
GGGCAGATCCTCGCCGGCGCGCGCGACATGATCGCCGGGGTGATGCGCAGCAAGACCGGCATGGGCGGCCAG 
CTCGCCTGGCCGGCGCTGCTGCGCAAGCTCGACCAGCAGAACCCGGGCTACCGCCAGTGA