ViewGene

Locus Name:
GeneID:
Location:

GeneID:3035
TrivialName: PA14_17210
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17210
PAO1 Ortholog Locus:PA3644
Sequence Length:777
Protein Length:258
Start: 1475049
Stop: 1475825
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07032.1|AE004784_5 (AE004784) UDP-N-acetylglucosamine acyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 258/258 (100%), Positives = 258/258 (100%)
Sequence:
ATGAGTTTGATCGATCCTCGCGCCATCATCGATCCGAGCGCCAGGCTGGCGGCTGACGTCCAGGTGGGCCCG
TGGTCCATCGTCGGGGCGGAAGTGGAGATCGGTGAAGGGACGGTGATCGGTCCGCACGTGGTGCTCAAGGGC
CCCACGAAGATCGGCAAGCACAACCGCATCTACCaGTTTTCCAGCGTCGGCGAGGACACTCCCGACCTGAAA
TACAAGGGCGAGCCGACGCGCCTGGTGATCGGTGACCACAACGTGATCCGCGAAGGCGTGACCATCCATCGC
GGAACCGTCCAGGATCGCGCGGAAACCACCATTGGCGACCACAACCTGATCATGGCCTATGCGCACATCGGC
CATGACAGCGTGATCGGCAACCATTGCATCCTGGTGAACAACACGGCGCTGGCCGGGCATGTCCACGTGGAC
GACTGGGCGATCCTGTCCGGCTACACCCTGGTGCACCAGTACTGCCGGATCGGCGCGCACAGCTTTTCCGGC
ATGGGCAGCGCCATCGGCAAGGACGTCCCGGCCTACGTGACGGTCTTCGGCAACCCGGCCGAAGCCCGCAGC
ATGAACTTCGAAGGCATGCGTCGGCGCGGCTTCAGCAGCGAGGCCATCCATGCCCTGCGGCGTGCCTACAAG
GTGGTCTATCGCCAGGGACATACCGTCGAGGAAGCCCTGGCCGAGCTGGCCGAAAGCGCTGCCCAGTTCCCC
GAGGTCGCGGTGTTCCGTGATTCCATCCAGAGCGCAACCCGCGGCATCACCCGCTGA
Translation:
MSLIDPRAIIDPSARLAADVQVGPWSIVGAEVEIGEGTVIGPHVVLKGPTKIGKHNRIYQFSSVGEDTPDLK
YKGEPTRLVIGDHNVIREGVTIHRGTVQDRAETTIGDHNLIMAYAHIGHDSVIGNHCILVNNTALAGHVHVD
DWAILSGYTLVHQYCRIGAHSFSGMGSAIGKDVPAYVTVFGNPAEARSMNFEGMRRRGFSSEAIHALRRAYK
VVYRQGHTVEEALAELAESAAQFPEVAVFRDSIQSATRGITR*
AnnotationID:3043GeneID:3035
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 21:09:56
Gene Name:lpxA
Confidence Code:1
GeneProduct:UDP-N-acetylglucosamine acetyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name: acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Functional Category Confidence Code:5
COGs:COG1043
Secondary Functional Category(ies):
EC Number:2.3.1.129
Status:ACTIVE
Pathway:
Homology:
>gb|AAN67224.1|AE016349_5 (AE016779) acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine
           O-acyltransferase [Pseudomonas putida KT2440]
          Length = 258

 Score =  461 bits (1187), Expect = e-129
 Identities = 223/258 (86%), Positives = 239/258 (92%)

>gb|AAP15714.1| (AE016978) UDP-N-acetylglucosamine acetyltransferase [Shigella
           flexneri 2a str. 2457T]
          Length = 262

 Score =  285 bits (728), Expect = 2e-76
 Identities = 140/257 (54%), Positives = 174/257 (67%)

>gb|AAC73292.1| (AE000127) UDP-N-acetylglucosamine acetyltransferase; lipid A
           biosynthesis [Escherichia coli K12]
          Length = 262

 Score =  285 bits (728), Expect = 2e-76
 Identities = 140/257 (54%), Positives = 174/257 (67%)

Structural Features:
COG1043, LpxA, Acyl-[acyl carrier protein]
CD-Length = 260 residues,  99.2% aligned
Score =  379 bits (976), Expect = 3e-106
Identities = 153/258 (59%), Positives = 192/258 (74%)
Genomic Context:
Comment:
ReferenceID:6073
Author/Investigator(s): GenNotator
Title:
PubMed:
MedLine:
Source:
Reference Type:BLASTP
Data:
URL:
ReferenceID:23215
Author/Investigator(s): GenNotator
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:RPSBLAST
Data:
URL:
ReferenceID:31899
Author/Investigator(s):
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:Misc (text/plain)
Data:3035_cdd.html
URL:
ReferenceID:34210
Author/Investigator(s): Dotson GD, Kaltashov IA, Cotter RJ, Raetz CR.
Title: Expression cloning of a Pseudomonas gene encoding a hydroxydecanoyl-acyl carrier protein-dependent UDP-GlcNAc acyltransferase.
PubMed: 9440522
MedLine:
Source:
J Bacteriol. 1998 Jan;180(2):330-7
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9440522
ReferenceID:34211
Author/Investigator(s): Steeghs L, Jennings MP, Poolman JT, van der Ley P.
Title: Isolation and characterization of the Neisseria meningitidis lpxD-fabZ-lpxA gene cluster involved in lipid A biosynthesis.
PubMed: 9197543
MedLine:
Source:
Gene. 1997 May 6;190(2):263-70.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9197543
ReferenceID:34212
Author/Investigator(s): Foster SJ.
Title: Purification and characterization of an 'actomyosin' complex from Escherichia coli W3110.
PubMed: 8354462
MedLine:
Source:
FEMS Microbiol Lett. 1993 Jul 1;110(3):295-8.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8354462
ReferenceID:34213
Author/Investigator(s): Coleman J, Raetz CR.
Title: First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.
PubMed: 3277952
MedLine:
Source:
J Bacteriol. 1988 Mar;170(3):1268-74.
Reference Type:Journal
Data:
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3277952
Homologs By Global Alignment
Gene ID:3035

Identity:

0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score
4901 PA3644_tr translation of PA3644 258 99.99 99.99 0 1340.0
138840 gb|AAG07032.1|AE004784_5 (AE004784) UDP-N-acetylglucosamine acyltransferase [Pseudomonas aeruginosa PAO1] 258 99.99 99.99 0 1340.0
138841 gb|AAD30149.1|AF142597_1 (AF142597) hydroxydecanoyl-acyl carrier protein-dependent UDP-N-acetylglucosamine-3-O-acyltransferase [Pseudomonas aeruginosa] 258 99.99 99.99 0 1340.0
138842 gb|AAO55066.1| (AE016861) acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase [Pseudomonas syringae pv. tomato str. DC3000] 258 87.59 93.79 0 1192.0
138843 gb|AAN67224.1|AE016349_5 (AE016779) acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase [Pseudomonas putida KT2440] 258 86.43 92.63 0 1187.0
GC ORFID: 46037How Found: BLASTX
GC_TrimmedSeqID: 69Blast Result ID
Subject Sequence Name: t_PA3644Glimmer Score:
Start: 3478888Stop: 3478112
Length: 777
Start Codon: ATGTruncated Start:
Stop Codon: TGATruncated Stop:
Homolog: t_PA3644 translation of PA3644Homolog Bit Score 520.0
Other Homologs: psyr_15may02_Scaffold3_revised_gene4631 (ORF: BLASTX 464.0), t_PA3644 (ORF: GLIMMER)
GC ORF Sequence
ATGAGTTTGATCGATCCTCGCGCCATCATCGATCCGAGCGCCAGGCTGGCGGCTGACGTCCAGGTGGGCCCG
TGGTCCATCGTCGGGGCGGAAGTGGAGATCGGTGAAGGGACGGTGATCGGTCCGCACGTGGTGCTCAAGGGC
CCCACGAAGATCGGCAAGCACAACCGCATCTACCaGTTTTCCAGCGTCGGCGAGGACACTCCCGACCTGAAA
TACAAGGGCGAGCCGACGCGCCTGGTGATCGGTGACCACAACGTGATCCGCGAAGGCGTGACCATCCATCGC
GGAACCGTCCAGGATCGCGCGGAAACCACCATTGGCGACCACAACCTGATCATGGCCTATGCGCACATCGGC
CATGACAGCGTGATCGGCAACCATTGCATCCTGGTGAACAACACGGCGCTGGCCGGGCATGTCCACGTGGAC
GACTGGGCGATCCTGTCCGGCTACACCCTGGTGCACCAGTACTGCCGGATCGGCGCGCACAGCTTTTCCGGC
ATGGGCAGCGCCATCGGCAAGGACGTCCCGGCCTACGTGACGGTCTTCGGCAACCCGGCCGAAGCCCGCAGC
ATGAACTTCGAAGGCATGCGTCGGCGCGGCTTCAGCAGCGAGGCCATCCATGCCCTGCGGCGTGCCTACAAG
GTGGTCTATCGCCAGGGACATACCGTCGAGGAAGCCCTGGCCGAGCTGGCCGAAAGCGCTGCCCAGTTCCCC
GAGGTCGCGGTGTTCCGTGATTCCATCCAGAGCGCAACCCGCGGCATCACCCGCTGA