ViewGene
Locus Name:
GeneID:
Location:

GeneID:3024
TrivialName: PA14_41170
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41170
PAO1 Ortholog Locus:PA1806
Sequence Length:798
Protein Length:265
Start: 3672654
Stop: 3673451
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05195.1|AE004607_1 (AE004607) NADH-dependent enoyl-ACP reductase [Pseudomonas aeruginosa PAO1]
Identities = 264/265 (99%), Positives = 265/265 (100%)
Sequence:
ATGGGATTTCTCACAGGAAAACGCGCCCTGATCGTCGGAGTCGCCAGCAAGCTCTCCATCGCTTCCGGCATC
GCCGCCGCCATGCACCGGGAAGGCGCCGAACTGGCCTTCACCTACCAGAACGACAAGCTCAGGGGCCGGGTG
GAGGAGTTCGCCAGTGGCTGGGGCTCGCGGCCGGAGCTGTGCTTCCCCTGTGACGTGGCCGACGACAGCCAG
ATCGAAGCGGTGTTCGCCGCCCTGGGCAAGCACTGGGACGGCCTGGACATCATCGTCCACTCCGTCGGATTC
GCTCCAGGCGATCAGCTCGACGGCGACTTCACCGCGGTGACCACCCGCGAGGGCTTCCGCATCGCCCACAAC
ATCAGCGCCTACAGCTTCATCGCCCTGGCCAAGGCCGGACGGGAAATGATGAAGGGCCGCAACGGCAGCCTG
CTGACCCTCTCCTACCTGGGCGCCGAACGGACCATGCCGAACTACAACGTAATGGGCATGGCCAAGGCCAGC
CTGGAGGCCGGGGTCCGCTACCTGGCCGGCAGCCTGGGCGCGGAAGGCACCCGGGTCAATGCGGTTTCCGCC
GGGCCGATCCGAACGCTCGCCGCCTCCGGGATCAAGAGTTTCCGCAAGATGCTGGCCGCCAACGAGCGGCAG
ACGCCGCTGCGGCGCAACGTCACCATCGAGGAAGTCGGCAATGCCGGCGCCTTCCTCTGTTCAGACCTGGCC
AGCGGTATCAGTGGCGAGATCCTCTACGTCgatggcGGCTTCAACACTACCGCCATGGGCCCGCTGGACGAC
GACTGA
Translation:
MGFLTGKRALIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLRGRVEEFASGWGSRPELCFPCDVADDSQ
IEAVFAALGKHWDGLDIIVHSVGFAPGDQLDGDFTAVTTREGFRIAHNISAYSFIALAKAGREMMKGRNGSL
LTLSYLGAERTMPNYNVMGMAKASLEAGVRYLAGSLGAEGTRVNAVSAGPIRTLAASGIKSFRKMLAANERQ
TPLRRNVTIEEVGNAGAFLCSDLASGISGEILYVDGGFNTTAMGPLDDD*
AnnotationID:3032GeneID:3024
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 13:01:56
Gene Name:fabI
Confidence Code:1
GeneProduct:NADH-dependent enoyl-ACP reductase
Cell Localization:()
Synonyms:envM
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0623
Secondary Functional Category(ies):
EC Number:1.3.1.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57190.1| (AE016869) enoyl-(acyl-carrier-protein) reductase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 264

 Score =  474 bits (1221), Expect = e-133
 Identities = 237/264 (89%), Positives = 248/264 (93%)

>gb|AAO69240.1| (AE016839) enoyl-[acyl-carrier-protein] reductase (NADH)
           [Salmonella enterica subsp. enterica serovar Typhi Ty2]
          Length = 262

 Score =  359 bits (921), Expect = 1e-98
 Identities = 182/263 (69%), Positives = 216/263 (82%), Gaps = 3/263 (1%)

>gb|AAN80225.1|AE016760_84 (AE016760) Enoyl-[acyl-carrier-protein] reductase [NADH]
           [Escherichia coli CFT073]
          Length = 262

 Score =  357 bits (917), Expect = 3e-98
 Identities = 181/263 (68%), Positives = 216/263 (82%), Gaps = 3/263 (1%)








 
Structural Features:
COG0623, FabI, Enoyl-[acyl-carrier-protein]
CD-Length = 259 residues, 100.0% aligned
Score =  382 bits (983), Expect = 2e-107
Identities = 167/263 (63%), Positives = 198/263 (75%), Gaps = 4/263 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6051




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23193




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37129




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3024_cdd.html


    		




 
URL:

















    ReferenceID:37798




  
Author/Investigator(s):
        Hoang TT, Schweizer HP.





  
Title:
        Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): a target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis.




  
PubMed:
        10464225


MedLine:
        




    
Source:
J Bacteriol. 1999 Sep;181(17):5489-97.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10464225









           
Homologs By Global Alignment 

           

       



     
Gene ID:3024
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4888
                           PA1806_tr
                           translation of PA1806
                           265
                           99.62
                           99.99
                           0
                           1342.0
     

     
     
138538
                           gb|AAG05195.1|AE004607_1
                           (AE004607) NADH-dependent enoyl-ACP reductase [Pseudomonas aeruginosa PAO1]
                           265
                           99.62
                           99.99
                           0
                           1342.0
     

     
     
138539
                           gb|AAC95362.1|
                           (AF104262) enoyl-(acyl-carrier protein) reductase [Pseudomonas aeruginosa]
                           265
                           99.62
                           99.99
                           0
                           1342.0
     

     
     
138540
                           gb|AAO57190.1|
                           (AE016869) enoyl-(acyl-carrier-protein) reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           265
                           89.43
                           93.58
                           1
                           1221.0
     

     
     
138542
                           emb|CAD01621.1|
                           (AL627270) enoyl-[acyl-carrier-protein] reductase (NADH) [Salmonella enterica subsp. enterica serovar Typhi]
                           265
                           68.67
                           81.50
                           3
                           920.0
     

     






      

          
GC ORFID: 46026How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1806Glimmer Score: 

          
Start: 1281255Stop: 1280458

          
Length: 798


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1806 translation of PA1806Homolog Bit Score 521.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene639 (ORF: BLASTX 475.0), t_PA1806 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGATTTCTCACAGGAAAACGCGCCCTGATCGTCGGAGTCGCCAGCAAGCTCTCCATCGCTTCCGGCATC 
GCCGCCGCCATGCACCGGGAAGGCGCCGAACTGGCCTTCACCTACCAGAACGACAAGCTCAGGGGCCGGGTG 
GAGGAGTTCGCCAGTGGCTGGGGCTCGCGGCCGGAGCTGTGCTTCCCCTGTGACGTGGCCGACGACAGCCAG 
ATCGAAGCGGTGTTCGCCGCCCTGGGCAAGCACTGGGACGGCCTGGACATCATCGTCCACTCCGTCGGATTC 
GCTCCAGGCGATCAGCTCGACGGCGACTTCACCGCGGTGACCACCCGCGAGGGCTTCCGCATCGCCCACAAC 
ATCAGCGCCTACAGCTTCATCGCCCTGGCCAAGGCCGGACGGGAAATGATGAAGGGCCGCAACGGCAGCCTG 
CTGACCCTCTCCTACCTGGGCGCCGAACGGACCATGCCGAACTACAACGTAATGGGCATGGCCAAGGCCAGC 
CTGGAGGCCGGGGTCCGCTACCTGGCCGGCAGCCTGGGCGCGGAAGGCACCCGGGTCAATGCGGTTTCCGCC 
GGGCCGATCCGAACGCTCGCCGCCTCCGGGATCAAGAGTTTCCGCAAGATGCTGGCCGCCAACGAGCGGCAG 
ACGCCGCTGCGGCGCAACGTCACCATCGAGGAAGTCGGCAATGCCGGCGCCTTCCTCTGTTCAGACCTGGCC 
AGCGGTATCAGTGGCGAGATCCTCTACGTCgatggcGGCTTCAACACTACCGCCATGGGCCCGCTGGACGAC 
GACTGA