ViewGene
Locus Name:
GeneID:
Location:

GeneID:3020
TrivialName: PA14_20330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20330
PAO1 Ortholog Locus:PA3382
Sequence Length:795
Protein Length:264
Start: 1750250
Stop: 1751044
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06770.1|AE004759_17 (AE004759) phosphonate transport protein PhnE [Pseudomonas aeruginosa PAO1]
Identities = 264/264 (100%), Positives = 264/264 (100%)
Sequence:
ATGAGCACTCTGAGCACCACCGCCGCGGCGGCCGCCACCGGCAAGCCATCCTGGGGACGCCTGGTCGGCTGG
GGCCTGTTCCTCGCCATCCTCGCCTGGTCCTGGAAGGGCGCGGAAATGAACCCGGCCGTGCTGTTCCGCGAC
GCCGGCAACATGGCGACCTTCGCCGCCGACTTCTTCCCGCCGGACTTCGGCAACTGGAAGCTCTACCTCAGC
GAGATGCTGGTCACCGTGCAGATCGCCCTCTGGGGCACGGTGCTGGCCATCGTCTGCGCTATCCCGCTGTCG
ATCCTGGCCTCCGAGAACATCGTGCCCTGGTGGGTCTACCAGCCGGTGCGGCGGGTGATGGATGCCTGCCGT
TCGATCAACGAGATGGTCTTCGCCATGCTCTTCGTGGTCGCCGTCGGCCTGGGCCCGTTCGCCGGCGTGCTG
GCGCTGTTCATCAGCACCACCGGGGTGCTCGCCAAGCTCTTCGCCGAGGCGGTCGAGGCGATCGACCCCGGT
CCGGTCGAAGGCGTCCGCGCCACCGGTGCCAGCGCCCTGCAGGAGGTCATCTTCGGAGTCATCCCGCAGGTC
CTGCCGCTGTGGATCTCCTATTCGCTGTACCGCTTCGAGTCCAACGTGCGTTCCGCCACCGTGGTCGGGATG
GTCGGCGCCGGCGGCATCGGCGTGATCCTCTGGGAGGCCATCCGCGGCTTCCAGTTCGGCCAGACCTGCGCC
GTGCTGATCGTCATCATCGTGGTGGTGAGCCTGATCGACGTTCTGTCACAACGCCTGCGCAAGCTGTTCATC
TGA
Translation:
MSTLSTTAAAAATGKPSWGRLVGWGLFLAILAWSWKGAEMNPAVLFRDAGNMATFAADFFPPDFGNWKLYLS
EMLVTVQIALWGTVLAIVCAIPLSILASENIVPWWVYQPVRRVMDACRSINEMVFAMLFVVAVGLGPFAGVL
ALFISTTGVLAKLFAEAVEAIDPGPVEGVRATGASALQEVIFGVIPQVLPLWISYSLYRFESNVRSATVVGM
VGAGGIGVILWEAIRGFQFGQTCAVLIVIIVVVSLIDVLSQRLRKLFI*
AnnotationID:3028GeneID:3020
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 07:07:09
Gene Name:phnE
Confidence Code:2
GeneProduct:phosphonate ABC tranporter permease protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:membrane channel protein component of Pn transporter
Functional Category Confidence Code:5
COGs:COG3639
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56062.1| (AE016865) phosphonates ABC transporter, permease protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 262

 Score =  415 bits (1067), Expect = e-115
 Identities = 205/257 (79%), Positives = 227/257 (88%)

>gb|AAG59303.1|AE005643_10 (AE005643) membrane channel protein component of Pn transporter
           [Escherichia coli O157:H7 EDL933]
          Length = 276

 Score =  392 bits (1007), Expect = e-108
 Identities = 188/253 (74%), Positives = 222/253 (87%)

>dbj|BAB73926.1| (AP003588) phosphonate ABC tranporter permease protein [Nostoc sp.
           PCC 7120]
          Length = 274

 Score =  302 bits (774), Expect = 1e-81
 Identities = 139/236 (58%), Positives = 193/236 (81%)








 
Structural Features:
COG3639, ABC-type phosphate/phosphonate transport system, 
permease component 
[Inorganic ion transport and metabolism]
CD-Length = 283 residues,  95.4% aligned
Score =  245 bits (626), Expect = 5e-66
Identities = 128/270 (47%), Positives = 179/270 (66%), Gaps = 6/270 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6043




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23185




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32179




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3020_cdd.html


    		




 
URL:

















    ReferenceID:34027




  
Author/Investigator(s):
        Elashvili I, Defrank JJ, Culotta VC.





  
Title:
        phnE and glpT genes enhance utilization of organophosphates in Escherichia coli K-12.




  
PubMed:
        9647836


MedLine:
        




    
Source:
Appl Environ Microbiol. 1998 Jul;64(7):2601-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9647836

















    ReferenceID:34028




  
Author/Investigator(s):
        Wanner BL, Metcalf WW.





  
Title:
        Molecular genetic studies of a 10.9-kb operon in Escherichia coli for phosphonate uptake and biodegradation.




  
PubMed:
        1335942


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1992 Dec 15;79(1-3):133-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1335942

















    ReferenceID:34029




  
Author/Investigator(s):
        Wanner BL.





  
Title:
        Genes for phosphonate biodegradation in Escherichia coli.




  
PubMed:
        1368181


MedLine:
        




    
Source:
SAAS Bull Biochem Biotechnol. 1992 Jan;5:1-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1368181

















    ReferenceID:34030




  
Author/Investigator(s):
        Chen CM, Ye QZ, Zhu ZM, Wanner BL, Walsh CT.





  
Title:
        Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B.




  
PubMed:
        2155230


MedLine:
        




    
Source:
J Biol Chem. 1990 Mar 15;265(8):4461-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2155230









           
Homologs By Global Alignment 

           

       



     
Gene ID:3020
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4881
                           PA3382_tr
                           translation of PA3382
                           264
                           99.99
                           99.99
                           0
                           1344.0
     

     
     
138496
                           gb|AAG06770.1|AE004759_17
                           (AE004759) phosphonate transport protein PhnE [Pseudomonas aeruginosa PAO1]
                           264
                           99.99
                           99.99
                           0
                           1344.0
     

     
     
138497
                           gb|AAO56062.1|
                           (AE016865) phosphonates ABC transporter, permease protein [Pseudomonas syringae pv. tomato str. DC3000]
                           265
                           77.73
                           86.79
                           4
                           1065.0
     

     
     
138501
                           gb|AAN83533.1|AE016771_44
                           (AE016771) membrane channel protein component of Pn transporter [Escherichia coli CFT073]
                           280
                           68.21
                           80.71
                           16
                           1000.0
     

     
     
138498
                           gb|AAG59303.1|AE005643_10
                           (AE005643) membrane channel protein component of Pn transporter [Escherichia coli O157:H7 EDL933]
                           281
                           67.61
                           80.42
                           22
                           1007.0
     

     






      

          
GC ORFID: 46022How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3382Glimmer Score: 

          
Start: 3203682Stop: 3202888

          
Length: 795


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3382 translation of PA3382Homolog Bit Score 522.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1218 (ORF: BLASTX 413.0), t_PA3382 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCACTCTGAGCACCACCGCCGCGGCGGCCGCCACCGGCAAGCCATCCTGGGGACGCCTGGTCGGCTGG 
GGCCTGTTCCTCGCCATCCTCGCCTGGTCCTGGAAGGGCGCGGAAATGAACCCGGCCGTGCTGTTCCGCGAC 
GCCGGCAACATGGCGACCTTCGCCGCCGACTTCTTCCCGCCGGACTTCGGCAACTGGAAGCTCTACCTCAGC 
GAGATGCTGGTCACCGTGCAGATCGCCCTCTGGGGCACGGTGCTGGCCATCGTCTGCGCTATCCCGCTGTCG 
ATCCTGGCCTCCGAGAACATCGTGCCCTGGTGGGTCTACCAGCCGGTGCGGCGGGTGATGGATGCCTGCCGT 
TCGATCAACGAGATGGTCTTCGCCATGCTCTTCGTGGTCGCCGTCGGCCTGGGCCCGTTCGCCGGCGTGCTG 
GCGCTGTTCATCAGCACCACCGGGGTGCTCGCCAAGCTCTTCGCCGAGGCGGTCGAGGCGATCGACCCCGGT 
CCGGTCGAAGGCGTCCGCGCCACCGGTGCCAGCGCCCTGCAGGAGGTCATCTTCGGAGTCATCCCGCAGGTC 
CTGCCGCTGTGGATCTCCTATTCGCTGTACCGCTTCGAGTCCAACGTGCGTTCCGCCACCGTGGTCGGGATG 
GTCGGCGCCGGCGGCATCGGCGTGATCCTCTGGGAGGCCATCCGCGGCTTCCAGTTCGGCCAGACCTGCGCC 
GTGCTGATCGTCATCATCGTGGTGGTGAGCCTGATCGACGTTCTGTCACAACGCCTGCGCAAGCTGTTCATC 
TGA