ViewGene
Locus Name:
GeneID:
Location:

GeneID:3012
TrivialName: PA14_20170
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20170
PAO1 Ortholog Locus:PA3395
Sequence Length:828
Protein Length:275
Start: 1738169
Stop: 1737342
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06783.1|AE004760_11 (AE004760) NosY protein [Pseudomonas aeruginosa PAO1]
Identities = 274/275 (99%), Positives = 274/275 (99%)
Sequence:
ATGCCCGTAGTCTGGACCATCGCCCGCAAGGAACTGGCCGACGGCCTGCGCAATCGCTGGCTGCTGGCGATC
AGCCTGCTGTTCGCCCTGCTCTCGGTGGGCATCGCCTGGTTCGGCGCCGCCGCGGCCGGCCAGGTCGGCTTC
ACCTCGGTGCCGGCGACGGTCGCCAGCCTGACCAGCCTGGCCACCTTCCTGATGCCGCTGATCGCCCTGCTG
CTGGCCTACGACGCCATCGTCGGCGAGGAGGAAGGCGGCACCCTGCTGCTGCTCCTGACCTACCCGCTGGGG
CGCGGCCAGTTGCTGCTCGGCAAGTTCCTCGGTCACGGCCTGATCCTCGCCCTGGCCACCCTGATCGGCTTC
GGCAGCGCGGCGCTGGCGATCCTCGTGCTGGTGCCGGAGGTCGAGGCGGCCATCCTGCTGGGCGCCTTCGGC
CGCTTCATGGGTTCCTCGCTGCTGCTCGGCTGCGTGTTCCTCGCCCTGGCCTACGCCCTGAGCAGCCGCGCC
AGCGAGAAATCCAGCGCCGCCGGGCAGGCGCTCGGCCTGTGGTTCTTCTTCGTCCTGCTGTTCGACCTGGCC
CTGCTGGCGATCCTGGTCCTCAGCCAGGGTCACCTGAGCCCGCGGCTGCTGCCCTGGCTGTTGCTGCTCAAC
CCGACCGACCTCTACCGGCTGATCAACCTGTCCGGCTTCGACGCAGCCGCCGCCGGCGGGGTGGTTCCCCTG
GCCAGCGACCTGCCGGTGTCCGCCAGCGCCCTCTGGCTGGCCCTGGCCCTCTGGGCCGGCGCTGCCCTGGCG
CTGGCCCACGGCCTGTTCCGCCGCCGCCCCATCTGA
Translation:
MPVVWTIARKELADGLRNRWLLAISLLFALLSVGIAWFGAAAAGQVGFTSVPATVASLTSLATFLMPLIALL
LAYDAIVGEEEGGTLLLLLTYPLGRGQLLLGKFLGHGLILALATLIGFGSAALAILVLVPEVEAAILLGAFG
RFMGSSLLLGCVFLALAYALSSRASEKSSAAGQALGLWFFFVLLFDLALLAILVLSQGHLSPRLLPWLLLLN
PTDLYRLINLSGFDAAAAGGVVPLASDLPVSASALWLALALWAGAALALAHGLFRRRPI*
AnnotationID:3020GeneID:3012
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 09:09:06
Gene Name:nosY
Confidence Code:2
GeneProduct:nitrous-oxide reductase, nosY component
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1277
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAC00877.1| (AB054991) NosY [Pseudomonas sp. MT-1]
          Length = 275

 Score =  404 bits (1039), Expect = e-112
 Identities = 204/274 (74%), Positives = 234/274 (85%)

>gb|AAG34389.1|AF197468_5 (AF197468) NosY [Pseudomonas fluorescens]
          Length = 275

 Score =  391 bits (1005), Expect = e-108
 Identities = 207/274 (75%), Positives = 233/274 (85%), Gaps = 2/274 (0%)

>dbj|BAC45583.1| (AP005936) nitrous oxide metabolic protein [Bradyrhizobium
           japonicum USDA 110]
          Length = 275

 Score =  247 bits (630), Expect = 6e-65
 Identities = 132/276 (47%), Positives = 185/276 (67%), Gaps = 2/276 (0%)








 
Structural Features:
COG1277, NosY, ABC-type transport system involved 
in multi-copper enzyme maturation, permease component
CD-Length = 278 residues,  99.6% aligned
Score = 79.4 bits (195), Expect = 4e-16
Identities = 87/280 (31%), Positives = 140/280 (50%), Gaps = 8/280 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6027




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23169




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32168




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3012_cdd.html


    		




 
URL:

















    ReferenceID:34057




  
Author/Investigator(s):
        Velasco L, Mesa S, Xu CA, Delgado MJ, Bedmar EJ.





  
Title:
        Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum.




  
PubMed:
        15028871


MedLine:
        




    
Source:
Antonie Van Leeuwenhoek. 2004 Apr;85(3):229-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15028871

















    ReferenceID:34058




  
Author/Investigator(s):
        Honisch U, Zumft WG.





  
Title:
        Operon structure and regulation of the nos gene region of Pseudomonas stutzeri, encoding an ABC-Type ATPase for maturation of nitrous oxide reductase.




  
PubMed:
        12618453


MedLine:
        




    
Source:
J Bacteriol. 2003 Mar;185(6):1895-902.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12618453

















    ReferenceID:34059




  
Author/Investigator(s):
        McGuirl MA, Nelson LK, Bollinger JA, Chan YK, Dooley DM.





  
Title:
        The nos (nitrous oxide reductase) gene cluster from the soil bacterium Achromobacter cycloclastes: cloning, sequence analysis, and expression.




  
PubMed:
        9720302


MedLine:
        




    
Source:






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9720302

















    ReferenceID:34060




  
Author/Investigator(s):
        Chan YK, McCormick WA, Watson RJ.





  
Title:
        A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti.




  
PubMed:
        9274035


MedLine:
        




    
Source:
Microbiology. 1997 Aug;143 ( Pt 8):2817-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9274035

















    ReferenceID:34061




  
Author/Investigator(s):
        Zumft WG, Viebrock-Sambale A, Braun C.





  
Title:
        Nitrous oxide reductase from denitrifying Pseudomonas stutzeri. Genes for copper-processing and properties of the deduced products, including a new member of the family of ATP/GTP-binding proteins.




  
PubMed:
        2170125


MedLine:
        




    
Source:
Eur J Biochem. 1990 Sep 24;192(3):591-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2170125









           
Homologs By Global Alignment 

           

       



     
Gene ID:3012
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4872
                           PA3395_tr
                           translation of PA3395
                           275
                           99.63
                           99.63
                           0
                           1346.0
     

     
     
138351
                           gb|AAG06783.1|AE004760_11
                           (AE004760) NosY protein [Pseudomonas aeruginosa PAO1]
                           275
                           99.63
                           99.63
                           0
                           1346.0
     

     
     
138354
                           gb|AAG34389.1|AF197468_5
                           (AF197468) NosY [Pseudomonas fluorescens]
                           277
                           75.09
                           84.47
                           4
                           1006.0
     

     
     
138352
                           dbj|BAC00877.1|
                           (AB054991) NosY [Pseudomonas sp. MT-1]
                           275
                           74.18
                           85.09
                           0
                           1038.0
     

     
     
138353
                           emb|CAA37717.1|
                           (X53676) nosY [Pseudomonas stutzeri]
                           276
                           73.55
                           84.42
                           1
                           1013.0
     

     






      

          
GC ORFID: 46014How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3395Glimmer Score: 

          
Start: 3215763Stop: 3216590

          
Length: 828


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3395 translation of PA3395Homolog Bit Score 523.0

          
Other Homologs:  t_PA3395 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCGTAGTCTGGACCATCGCCCGCAAGGAACTGGCCGACGGCCTGCGCAATCGCTGGCTGCTGGCGATC 
AGCCTGCTGTTCGCCCTGCTCTCGGTGGGCATCGCCTGGTTCGGCGCCGCCGCGGCCGGCCAGGTCGGCTTC 
ACCTCGGTGCCGGCGACGGTCGCCAGCCTGACCAGCCTGGCCACCTTCCTGATGCCGCTGATCGCCCTGCTG 
CTGGCCTACGACGCCATCGTCGGCGAGGAGGAAGGCGGCACCCTGCTGCTGCTCCTGACCTACCCGCTGGGG 
CGCGGCCAGTTGCTGCTCGGCAAGTTCCTCGGTCACGGCCTGATCCTCGCCCTGGCCACCCTGATCGGCTTC 
GGCAGCGCGGCGCTGGCGATCCTCGTGCTGGTGCCGGAGGTCGAGGCGGCCATCCTGCTGGGCGCCTTCGGC 
CGCTTCATGGGTTCCTCGCTGCTGCTCGGCTGCGTGTTCCTCGCCCTGGCCTACGCCCTGAGCAGCCGCGCC 
AGCGAGAAATCCAGCGCCGCCGGGCAGGCGCTCGGCCTGTGGTTCTTCTTCGTCCTGCTGTTCGACCTGGCC 
CTGCTGGCGATCCTGGTCCTCAGCCAGGGTCACCTGAGCCCGCGGCTGCTGCCCTGGCTGTTGCTGCTCAAC 
CCGACCGACCTCTACCGGCTGATCAACCTGTCCGGCTTCGACGCAGCCGCCGCCGGCGGGGTGGTTCCCCTG 
GCCAGCGACCTGCCGGTGTCCGCCAGCGCCCTCTGGCTGGCCCTGGCCCTCTGGGCCGGCGCTGCCCTGGCG 
CTGGCCCACGGCCTGTTCCGCCGCCGCCCCATCTGA