ViewGene
Locus Name:
GeneID:
Location:

GeneID:301
TrivialName: PA14_39690
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_39690
PAO1 Ortholog Locus:PA1920
Sequence Length:2028
Protein Length:675
Start: 3535976
Stop: 3538003
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05308.1|AE004618_2 (AE004618) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 673/675 (99%), Positives = 674/675 (99%)
Sequence:
ATGATCCAGCCCCGGAAGGCTACCTGGCCGGCCACCCTGCTCAAGCGCAACGGACGTGAAGTCCCCTTCGAT
CCCGGCAAGATCGAACGCGCCATCGCCGCCGCCGGCCAGGCCACCGGCGAGTTCGACCCGCAGCAGGCGCAG
GCGCTCGCCGAACGCGTGGTGGAACGCCTGCCGGAACGCGCCTGCCTGGATATCGAACGGGTCCAGGACCTG
GTCGAACGGGTGCTGATGGAGGCCGGCCACTACCCCACGGCGCGCGCCTACGTGGTCTACCGCGAACGCCAC
GGCCGGCTGCGCCGCGAGCGCAAGACCCTGGTGGACGTCGCCGCGTCGATGAACGAATACCTTTCCCGCGAG
GATTGGCGGGTGCGCGCCAACGCCAACCAGGGCTATTCCCTCGGCGGGCTGATCCTCAACGTATCCGGCAAG
GTCACCGCGAACTACTGGCTGGACGAGGTCTACGATGCCGACATCGCCGCCGCCCACCGCGACGCCGACCTG
CATATCCACGACCTCGACATGCTCGCCGGCTATTGCGCCGGCTGGTCGCTGCGCACCTTGCTGAACGAAGGC
CTGAACGGCGTGCCGGGGCGGGTcGAGGcCGGTCCGCCGAAGCACCTGgGCAGCGCCCTGGGGCAGATGGTC
AACTTCCTCGGCACCCTGCAGAACGAATGGGCCGGGGCCCAGGCCTTCAGTTCCTTCGATACCTACCTGGCG
CCCTACGTGCGCAAGGACCGCCTCGACTACGCCAGCGTGCGCCAGGCCCTCCAGGAGTTCATCTACAACCTC
AACGTGCCGTCGCGCTGGGGCACCCAGACGCCCTTCACCAACCTGACCTTCGACTGGGTCTGCCCGGACGAC
CTGCGCGACCAGGTGCCGCGCATCGGCGGCGAGGAGATGCCCTTCGCCTACGGCGAGCTGCAGGCCGAGATG
GACCTGATCAACCGCGCCTACATCGAGGTGATGCAGGCCGGCGACGCCCATGGCCGGGTGTTCACCTTCCCG
ATCCCGACCTACAACATCACCCACGACTTCCCCTGGGACAGCGACAACGCCACGCGCCTGTTCGAGATGACC
GCGCGCTACGGCCTGCCCTACTTCCAGAACTTCCTCAACTCGGACATGCAGCCCAACCAGGTGCGCTCGATG
TGCTGCCGCCTGCAACTGGACGTGCGCGAACTGCTCAAGCGCGGCAACGGCCTGTTCGGCTCGGCGGAGCAG
ACCGGCTCGCTGGGCGTGGTGACGATCAACTGCGCGCGCCTGGGCTATCGCCACCGTGGCGACGCCGCCGGC
CTCTACCGCCAGCTCGACCACCTGCTCGAACTCGCCTTCGCCAGCCTGGAGCTGAAGCGCAAGGTGATCCAG
CAGCACATGGATGCCGGCCTCTATCCCTACACCCGACGCTACCTGGGTACCCTGCGCAACCATTTCTCCACC
ATCGGCGTGAACGGCCTGCACGAGATGGTGCGCAACTTCAGCGACGACCGCGAGGGACTGCACAGCGAAGCC
GGGCGCGAGATGGCCCTGGCCCTGCTCGATCACGTGCGCGCGCGGCTGGTGGGTTTCCAGGAGAACAGCGGG
CACCTCTACAACCTGGAAGCCACGCCCGCCGAGGGCACCACCTATCGCTTCGCCCGCGAGGACCGCAAGCGC
TATCCGCGCATCCTCCAGGCCGGCACGCCGGAGGCGCCCTACTACACCAACTCCTCGCAACTGCCGGTGGGC
TTCACCGACGACCCGTTCGAGGCGCTGGAGCTGCAGGACGCCCTGCAATGCAAGTACACCGGCGGCACCGTG
CTGCATCTCTACATGGCCGAGCGGATTTCCTCCGCCGAAGCCTGCAAGACCCTGGTGCGCACCGCCCTCGGT
CGCTTCCGCCTGCCGTACCTGACCGTCACCCCCACCTTCTCGATCTGCCCGCGGCACGGCTACCTGGCCGGC
GAGCACGAGTTCTGCCCGAAGTGCGACGAGGAGCTGCTGCAACGCTCCACGCCCAAGAGCTGTTGCGGCGGC
TGCGGCGGTTGA
Translation:
MIQPRKATWPATLLKRNGREVPFDPGKIERAIAAAGQATGEFDPQQAQALAERVVERLPERACLDIERVQDL
VERVLMEAGHYPTARAYVVYRERHGRLRRERKTLVDVAASMNEYLSREDWRVRANANQGYSLGGLILNVSGK
VTANYWLDEVYDADIAAAHRDADLHIHDLDMLAGYCAGWSLRTLLNEGLNGVPGRVEAGPPKHLGSALGQMV
NFLGTLQNEWAGAQAFSSFDTYLAPYVRKDRLDYASVRQALQEFIYNLNVPSRWGTQTPFTNLTFDWVCPDD
LRDQVPRIGGEEMPFAYGELQAEMDLINRAYIEVMQAGDAHGRVFTFPIPTYNITHDFPWDSDNATRLFEMT
ARYGLPYFQNFLNSDMQPNQVRSMCCRLQLDVRELLKRGNGLFGSAEQTGSLGVVTINCARLGYRHRGDAAG
LYRQLDHLLELAFASLELKRKVIQQHMDAGLYPYTRRYLGTLRNHFSTIGVNGLHEMVRNFSDDREGLHSEA
GREMALALLDHVRARLVGFQENSGHLYNLEATPAEGTTYRFAREDRKRYPRILQAGTPEAPYYTNSSQLPVG
FTDDPFEALELQDALQCKYTGGTVLHLYMAERISSAEACKTLVRTALGRFRLPYLTVTPTFSICPRHGYLAG
EHEFCPKCDEELLQRSTPKSCCGGCGG*
AnnotationID:311GeneID:301
AnnotatorUID: diggins
Modification Date/Time: 2005-06-06 12:12:13
Gene Name:
Confidence Code:3
GeneProduct:putative ribonucleotide reductase
Cell Localization:()
Synonyms:nrdD
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:putative oxygen-sensitive ribonucleoside-triphosphate reductase
Functional Category Confidence Code:5
COGs:COG1328
Secondary Functional Category(ies):
EC Number:1.17.4.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAP85992.1| (AY305378) anaerobic class III ribonucleotide reductase [Ralstonia
           eutropha]
          Length = 676

 Score =  994 bits (2570), Expect = 0.0
 Identities = 478/660 (72%), Positives = 551/660 (83%), Gaps = 3/660 (0%)

>emb|CAD18114.1| (AL646082) PUTATIVE RIBONUCLEOTIDE REDUCTASE OXIDOREDUCTASE PROTEIN
           [Ralstonia solanacearum]
          Length = 683

 Score =  993 bits (2568), Expect = 0.0
 Identities = 475/659 (72%), Positives = 547/659 (83%), Gaps = 2/659 (0%)

>gb|AAM25039.1| (AE013136) Oxygen-sensitive ribonucleoside-triphosphate reductase
           [Thermoanaerobacter tengcongensis]
          Length = 692

 Score =  675 bits (1741), Expect = 0.0
 Identities = 335/657 (50%), Positives = 458/657 (69%), Gaps = 14/657 (2%)







 
Structural Features:
COG1328, NrdD, Oxygen-sensitive ribonucleoside-triphosphate reductase 
[Nucleotide transport and metabolism]
CD-Length = 700 residues,  96.7% aligned
Score =  651 bits (1682), Expect = 0.0
Identities = 200/689 (29%), Positives = 288/689 (41%), Gaps = 40/689 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:605




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17747




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37026




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:301_cdd.html


    		




 
URL:

















    ReferenceID:38185




  
Author/Investigator(s):
        Sun X, Harder J, Krook M, Jornvall H, Sjoberg BM, Reichard P.





  
Title:
        A possible glycine radical in anaerobic ribonucleotide reductase from Escherichia coli: nucleotide sequence of the cloned nrdD gene.




  
PubMed:
        8421692


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):577-81.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8421692









           
Homologs By Global Alignment 

           

       



     
Gene ID:301
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
493
                           PA1920_tr
                           translation of PA1920
                           675
                           99.70
                           99.85
                           0
                           3578.0
     

     
     
25155
                           gb|AAG05308.1|AE004618_2
                           (AE004618) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           675
                           99.70
                           99.85
                           0
                           3578.0
     

     
     
25156
                           gb|AAP85992.1|
                           (AY305378) anaerobic class III ribonucleotide reductase [Ralstonia eutropha]
                           681
                           70.19
                           81.20
                           11
                           2566.0
     

     
     
25157
                           emb|CAA10041.1|
                           (AJ012479) NrdD protein [Ralstonia eutropha]
                           681
                           70.19
                           81.20
                           11
                           2566.0
     

     
     
25158
                           emb|CAD18114.1|
                           (AL646082) PUTATIVE RIBONUCLEOTIDE REDUCTASE OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
                           692
                           68.64
                           79.19
                           26
                           2563.0
     

     






      

          
GC ORFID: 43303How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1920Glimmer Score: 

          
Start: 1417935Stop: 1415908

          
Length: 2028


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1920 translation of PA1920Homolog Bit Score 1382.0

          
Other Homologs:  t_PA1920 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCCAGCCCCGGAAGGCTACCTGGCCGGCCACCCTGCTCAAGCGCAACGGACGTGAAGTCCCCTTCGAT 
CCCGGCAAGATCGAACGCGCCATCGCCGCCGCCGGCCAGGCCACCGGCGAGTTCGACCCGCAGCAGGCGCAG 
GCGCTCGCCGAACGCGTGGTGGAACGCCTGCCGGAACGCGCCTGCCTGGATATCGAACGGGTCCAGGACCTG 
GTCGAACGGGTGCTGATGGAGGCCGGCCACTACCCCACGGCGCGCGCCTACGTGGTCTACCGCGAACGCCAC 
GGCCGGCTGCGCCGCGAGCGCAAGACCCTGGTGGACGTCGCCGCGTCGATGAACGAATACCTTTCCCGCGAG 
GATTGGCGGGTGCGCGCCAACGCCAACCAGGGCTATTCCCTCGGCGGGCTGATCCTCAACGTATCCGGCAAG 
GTCACCGCGAACTACTGGCTGGACGAGGTCTACGATGCCGACATCGCCGCCGCCCACCGCGACGCCGACCTG 
CATATCCACGACCTCGACATGCTCGCCGGCTATTGCGCCGGCTGGTCGCTGCGCACCTTGCTGAACGAAGGC 
CTGAACGGCGTGCCGGGGCGGGTcGAGGcCGGTCCGCCGAAGCACCTGgGCAGCGCCCTGGGGCAGATGGTC 
AACTTCCTCGGCACCCTGCAGAACGAATGGGCCGGGGCCCAGGCCTTCAGTTCCTTCGATACCTACCTGGCG 
CCCTACGTGCGCAAGGACCGCCTCGACTACGCCAGCGTGCGCCAGGCCCTCCAGGAGTTCATCTACAACCTC 
AACGTGCCGTCGCGCTGGGGCACCCAGACGCCCTTCACCAACCTGACCTTCGACTGGGTCTGCCCGGACGAC 
CTGCGCGACCAGGTGCCGCGCATCGGCGGCGAGGAGATGCCCTTCGCCTACGGCGAGCTGCAGGCCGAGATG 
GACCTGATCAACCGCGCCTACATCGAGGTGATGCAGGCCGGCGACGCCCATGGCCGGGTGTTCACCTTCCCG 
ATCCCGACCTACAACATCACCCACGACTTCCCCTGGGACAGCGACAACGCCACGCGCCTGTTCGAGATGACC 
GCGCGCTACGGCCTGCCCTACTTCCAGAACTTCCTCAACTCGGACATGCAGCCCAACCAGGTGCGCTCGATG 
TGCTGCCGCCTGCAACTGGACGTGCGCGAACTGCTCAAGCGCGGCAACGGCCTGTTCGGCTCGGCGGAGCAG 
ACCGGCTCGCTGGGCGTGGTGACGATCAACTGCGCGCGCCTGGGCTATCGCCACCGTGGCGACGCCGCCGGC 
CTCTACCGCCAGCTCGACCACCTGCTCGAACTCGCCTTCGCCAGCCTGGAGCTGAAGCGCAAGGTGATCCAG 
CAGCACATGGATGCCGGCCTCTATCCCTACACCCGACGCTACCTGGGTACCCTGCGCAACCATTTCTCCACC 
ATCGGCGTGAACGGCCTGCACGAGATGGTGCGCAACTTCAGCGACGACCGCGAGGGACTGCACAGCGAAGCC 
GGGCGCGAGATGGCCCTGGCCCTGCTCGATCACGTGCGCGCGCGGCTGGTGGGTTTCCAGGAGAACAGCGGG 
CACCTCTACAACCTGGAAGCCACGCCCGCCGAGGGCACCACCTATCGCTTCGCCCGCGAGGACCGCAAGCGC 
TATCCGCGCATCCTCCAGGCCGGCACGCCGGAGGCGCCCTACTACACCAACTCCTCGCAACTGCCGGTGGGC 
TTCACCGACGACCCGTTCGAGGCGCTGGAGCTGCAGGACGCCCTGCAATGCAAGTACACCGGCGGCACCGTG 
CTGCATCTCTACATGGCCGAGCGGATTTCCTCCGCCGAAGCCTGCAAGACCCTGGTGCGCACCGCCCTCGGT 
CGCTTCCGCCTGCCGTACCTGACCGTCACCCCCACCTTCTCGATCTGCCCGCGGCACGGCTACCTGGCCGGC 
GAGCACGAGTTCTGCCCGAAGTGCGACGAGGAGCTGCTGCAACGCTCCACGCCCAAGAGCTGTTGCGGCGGC 
TGCGGCGGTTGA