ViewGene
Locus Name:
GeneID:
Location:

GeneID:3002
TrivialName: PA14_03680
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03680
PAO1 Ortholog Locus:PA0282
Sequence Length:819
Protein Length:272
Start: 330784
Stop: 329966
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03671.1|AE004466_5 (AE004466) sulfate transport protein CysT [Pseudomonas aeruginosa PAO1]
Identities = 272/272 (100%), Positives = 272/272 (100%)
Sequence:
ATGTCACGACGCATTTCCCCGGTCATACCCGGCTTCGGGCTGACCCTGGGATACACCCTGGTGTACCTCAGC
CTGCTGGTCCTGATCCCGCTGGGGGCCATGTTCCTCAAGACCACCCAGCTCAGCTGGGAGCAATTCTGGGCC
ATCATCAGCGCGCCGCGGGTGCTCGCCGCGCTCAAGCTGAGCTTCGGCACCGCGCTCGCCGCCGCCGTGCTC
AATGGCTTGATCGGTACGCTGCTGGCCTGGGTCCTGGTGCGCTACGAATTCCCCGGACGGAAGATCATCGAC
GCCATGATCGACCTGCCCTTCGCCCTGCCCACCGCCGTCGCCGGTATCGCCCTCACCGCGCTCTATGCGCCG
GCCGGCCTGGTCGGCCAGTTCGCCAGCGACCTCGGCTTCAAGATCGCCTATACCCCCCTGGGGATCACCCTG
GCGCTGACCTTCGTGACCCTGCCGTTCGTGGTCCGCACGGTGCAGCCGGTGCTCGCCGACATTCCCAAGGAA
GTCGAGGAAGCCGCCGCCTGCCTCGGCGCCAGGCCGCTGCAGGTGTTCCGCCATATCCTCGTGCCGGCGCTG
CTGCCGGCCTGGCTGACCGGTTTCGCCCTGGCCTTCGCCCGGGGCGTCGGCGAATACGGCTCGGTGATCTTC
ATCGCCGGCAACATGCCGATGAAGACCGAGATCCTGCCGCTGCTGATCATGGTCAAGCTGGACCAGTACGAC
TACACCGGCGCCACCGCCATCGGCGTGCTGATGCTGGTGGTGTCCTTCATCCTGCTGCTGCTGATCAACCTG
CTGCAGCGTCGCATCGAAACCCCGTAA
Translation:
MSRRISPVIPGFGLTLGYTLVYLSLLVLIPLGAMFLKTTQLSWEQFWAIISAPRVLAALKLSFGTALAAAVL
NGLIGTLLAWVLVRYEFPGRKIIDAMIDLPFALPTAVAGIALTALYAPAGLVGQFASDLGFKIAYTPLGITL
ALTFVTLPFVVRTVQPVLADIPKEVEEAAACLGARPLQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIF
IAGNMPMKTEILPLLIMVKLDQYDYTGATAIGVLMLVVSFILLLLINLLQRRIETP*
AnnotationID:3010GeneID:3002
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 06:06:29
Gene Name:cysT
Confidence Code:2
GeneProduct:sulfate transport protein CysT
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:sulfate ABC transporter, permease protein
Functional Category Confidence Code:5
COGs:COG0555
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53854.1| (AE016857) sulfate ABC transporter, permease protein CysT
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 273

 Score =  491 bits (1264), Expect = e-138
 Identities = 249/272 (91%), Positives = 262/272 (96%)

>gb|AAN70735.1|AE016717_3 (AE016793) sulfate ABC transporter, permease protein [Pseudomonas
           putida KT2440]
          Length = 272

 Score =  491 bits (1263), Expect = e-138
 Identities = 251/272 (92%), Positives = 263/272 (96%)

>emb|CAD84489.1| (BX321858) cysU; sulfate transport ABC transporter protein
           [Nitrosomonas europaea ATCC 19718]
          Length = 277

 Score =  352 bits (904), Expect = 1e-96
 Identities = 170/259 (65%), Positives = 217/259 (83%)






 
Structural Features:
COG0555, CysU, ABC-type sulfate transport system, permease component 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 274 residues,  97.8% aligned
Score =  270 bits (691), Expect = 2e-73
Identities = 158/268 (58%), Positives = 207/268 (77%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6007




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23149




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33634




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3002_cdd.html


    		




 
URL:

















    ReferenceID:35442




  
Author/Investigator(s):
        Laudenbach DE, Grossman AR.





  
Title:
        Characterization and mutagenesis of sulfur-regulated genes in a cyanobacterium: evidence for function in sulfate transport.




  
PubMed:
        1708375


MedLine:
        




    
Source:
J Bacteriol. 1991 May;173(9):2739-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1708375

















    ReferenceID:35447




  
Author/Investigator(s):
        Sirko A, Hryniewicz M, Hulanicka D, Bock A.





  
Title:
        Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster.




  
PubMed:
        2188958


MedLine:
        




    
Source:
J Bacteriol. 1990 Jun;172(6):3351-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2188958









           
Homologs By Global Alignment 

           

       



     
Gene ID:3002
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4861
                           PA0282_tr
                           translation of PA0282
                           272
                           99.99
                           99.99
                           0
                           1351.0
     

     
     
138121
                           gb|AAG03671.1|AE004466_5
                           (AE004466) sulfate transport protein CysT [Pseudomonas aeruginosa PAO1]
                           272
                           99.99
                           99.99
                           0
                           1351.0
     

     
     
138123
                           gb|AAN70735.1|AE016717_3
                           (AE016793) sulfate ABC transporter, permease protein [Pseudomonas putida KT2440]
                           272
                           92.27
                           96.69
                           0
                           1263.0
     

     
     
138122
                           gb|AAO53854.1|
                           (AE016857) sulfate ABC transporter, permease protein CysT [Pseudomonas syringae pv. tomato str. DC3000]
                           273
                           91.20
                           95.97
                           1
                           1264.0
     

     
     
138124
                           emb|CAD84489.1|
                           (BX321858) cysU; sulfate transport ABC transporter protein [Nitrosomonas europaea ATCC 19718]
                           279
                           62.72
                           80.64
                           9
                           895.0
     

     






      

          
GC ORFID: 46004How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0282Glimmer Score: 

          
Start: 6132125Stop: 6131307

          
Length: 819


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0282 translation of PA0282Homolog Bit Score 525.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4049 (ORF: BLASTX 491.0), t_PA0282 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCACGACGCATTTCCCCGGTCATACCCGGCTTCGGGCTGACCCTGGGATACACCCTGGTGTACCTCAGC 
CTGCTGGTCCTGATCCCGCTGGGGGCCATGTTCCTCAAGACCACCCAGCTCAGCTGGGAGCAATTCTGGGCC 
ATCATCAGCGCGCCGCGGGTGCTCGCCGCGCTCAAGCTGAGCTTCGGCACCGCGCTCGCCGCCGCCGTGCTC 
AATGGCTTGATCGGTACGCTGCTGGCCTGGGTCCTGGTGCGCTACGAATTCCCCGGACGGAAGATCATCGAC 
GCCATGATCGACCTGCCCTTCGCCCTGCCCACCGCCGTCGCCGGTATCGCCCTCACCGCGCTCTATGCGCCG 
GCCGGCCTGGTCGGCCAGTTCGCCAGCGACCTCGGCTTCAAGATCGCCTATACCCCCCTGGGGATCACCCTG 
GCGCTGACCTTCGTGACCCTGCCGTTCGTGGTCCGCACGGTGCAGCCGGTGCTCGCCGACATTCCCAAGGAA 
GTCGAGGAAGCCGCCGCCTGCCTCGGCGCCAGGCCGCTGCAGGTGTTCCGCCATATCCTCGTGCCGGCGCTG 
CTGCCGGCCTGGCTGACCGGTTTCGCCCTGGCCTTCGCCCGGGGCGTCGGCGAATACGGCTCGGTGATCTTC 
ATCGCCGGCAACATGCCGATGAAGACCGAGATCCTGCCGCTGCTGATCATGGTCAAGCTGGACCAGTACGAC 
TACACCGGCGCCACCGCCATCGGCGTGCTGATGCTGGTGGTGTCCTTCATCCTGCTGCTGCTGATCAACCTG 
CTGCAGCGTCGCATCGAAACCCCGTAA