ViewGene
Locus Name:
GeneID:
Location:

GeneID:3000
TrivialName: PA14_71960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71960
PAO1 Ortholog Locus:PA5451
Sequence Length:798
Protein Length:265
Start: 6414586
Stop: 6413789
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08836.1|AE004958_6 (AE004958) membrane subunit of A-band LPS efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 265/265 (100%), Positives = 265/265 (100%)
Sequence:
ATGCTTCTTGGCTTGTCTCGTTCCTTGTGGGGCTATCGTGGCTTCGTTCTCGGCAGCGTCAAGCGCGAGTTC
CAGTCGCGCTACCGCGGGTCGCTGTTCGGCGCGCTGTGGACGGTGCTCAATCCGCTGTCGATGATCGTCGTC
TACACGGTGATCTTCTCCCAGGTGATGCGCGCGCGCCTGCCGGGCGTCGACGACGGCCTGGCCTACAGCGTC
TATCTCTGCGCCGGGCTGCTGACCTGGGGCCTGTTCGCGGAAATCACCTCGCGCAGCCAGAGCATGTTCATC
GAGAACGCCAACCTGCTGAAGAAGATCAGCTTCCCGCGCATCTGCCTGCCGGTCATCGTACTGCTCAACGCC
GGGGTCAATTTCGCCATCATCCTCGCCTTGTTCCTCGGCTTTCTCGCACTCAGCGGGCGCCTGCCGGGCGCG
GCATTGCTCGCGCTGGTGCCGCTGCTGGCGATCCAGGTGCTGTTCGCGGCGGGACTGGGGATGATCCTCGGG
GTGCTCAACGTGTTCTTCCGCGACGTCGGCCAGTTGTTCGGCATCTGTCTGCAGTTCTGGTTCTGGCTGACG
CCGATCGTCTATCCGATCGGCATCCTGCCCGAGGGTATCCGCTCGCTGATCGAACTCAACCCGATGACCGCG
CTGATGCGCAGCTACCAGCAGCTGTTCCTCCACGGGCAATGGCCGGACTGGCCTTCATTGCTGCCGATCACC
CTGCTGGCGCTGCTGCTGTGCGCGCTCGGCCTGCGTCTGTTCCGCCAGCGGGTCGGCGAAATGGTGGATGAA
CTCTGA
Translation:
MLLGLSRSLWGYRGFVLGSVKREFQSRYRGSLFGALWTVLNPLSMIVVYTVIFSQVMRARLPGVDDGLAYSV
YLCAGLLTWGLFAEITSRSQSMFIENANLLKKISFPRICLPVIVLLNAGVNFAIILALFLGFLALSGRLPGA
ALLALVPLLAIQVLFAAGLGMILGVLNVFFRDVGQLFGICLQFWFWLTPIVYPIGILPEGIRSLIELNPMTA
LMRSYQQLFLHGQWPDWPSLLPITLLALLLCALGLRLFRQRVGEMVDEL*
AnnotationID:3008GeneID:3000
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 20:08:49
Gene Name:wzm
Confidence Code:1
GeneProduct:membrane subunit of A-band LPS efflux transporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:integral membrane component of ABC-transporter
Functional Category Confidence Code:5
COGs:pfam01061,COG1682
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA28324.1| (AB010150) integral membrane component of ABC-transporter
           [Escherichia coli]
          Length = 264

 Score =  323 bits (829), Expect = 5e-88
 Identities = 148/261 (56%), Positives = 202/261 (77%)

>dbj|BAA28343.1| (AB010296) integral membrane component of ABC-transporter
           [Klebsiella pneumoniae]
          Length = 261

 Score =  294 bits (753), Expect = 3e-79
 Identities = 135/258 (52%), Positives = 188/258 (72%)






 
Structural Features:
pfam01061, ABC2_membrane, ABC-2 type transporter.
CD-Length = 254 residues,  99.6% aligned
Score =  211 bits (539), Expect = 6e-56
Identities = 118/256 (46%), Positives = 167/256 (65%), Gaps = 3/256 (1%)

COG1682, TagG, ABC-type polysaccharide/polyol phosphate export systems, 
permease component 
[Carbohydrate transport and metabolism / 
Cell envelope biogenesis, outer membrane]
CD-Length = 263 residues,  98.5% aligned
Identities = 113/265 (42%), Positives = 158/265 (59%), Gaps = 6/265 (2%)
Score =  186 bits (475), Expect = 2e-48








   
Genomic Context:






   
Comment:


















    ReferenceID:6003




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23145




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34652




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3000_cdd.html


    		




 
URL:

















    ReferenceID:35623




  
Author/Investigator(s):
        Godfroid F, Cloeckaert A, Taminiau B, Danese I, Tibor A, de Bolle X, Mertens P, Letesson JJ.





  
Title:
        Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of brucella melitensis 16M (wbk).




  
PubMed:
        11081580


MedLine:
        




    
Source:
Res Microbiol. 2000 Oct;151(8):655-68.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11081580









           
Homologs By Global Alignment 

           

       



     
Gene ID:3000
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4859
                           PA5451_tr
                           translation of PA5451
                           265
                           99.99
                           99.99
                           0
                           1352.0
     

     
     
138105
                           gb|AAG08836.1|AE004958_6
                           (AE004958) membrane subunit of A-band LPS efflux transporter [Pseudomonas aeruginosa PAO1]
                           265
                           99.99
                           99.99
                           0
                           1352.0
     

     
     
138106
                           gb|AAC16667.1|
                           (U63722) membrane-spanning domain MSD [Pseudomonas aeruginosa]
                           265
                           99.62
                           99.62
                           0
                           1348.0
     

     
     
138107
                           dbj|BAA28324.1|
                           (AB010150) integral membrane component of ABC-transporter [Escherichia coli]
                           265
                           55.84
                           76.60
                           1
                           828.0
     

     
     
138112
                           dbj|BAA28335.1|
                           (AB010294) integral membrane component of ABC-transporter [Escherichia coli]
                           265
                           50.94
                           71.32
                           4
                           748.0
     

     






      

          
GC ORFID: 46002How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5451Glimmer Score: 

          
Start: 5678275Stop: 5677478

          
Length: 798


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5451 translation of PA5451Homolog Bit Score 525.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4276 (ORF: BLASTX 254.0), t_PA5451 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTTCTTGGCTTGTCTCGTTCCTTGTGGGGCTATCGTGGCTTCGTTCTCGGCAGCGTCAAGCGCGAGTTC 
CAGTCGCGCTACCGCGGGTCGCTGTTCGGCGCGCTGTGGACGGTGCTCAATCCGCTGTCGATGATCGTCGTC 
TACACGGTGATCTTCTCCCAGGTGATGCGCGCGCGCCTGCCGGGCGTCGACGACGGCCTGGCCTACAGCGTC 
TATCTCTGCGCCGGGCTGCTGACCTGGGGCCTGTTCGCGGAAATCACCTCGCGCAGCCAGAGCATGTTCATC 
GAGAACGCCAACCTGCTGAAGAAGATCAGCTTCCCGCGCATCTGCCTGCCGGTCATCGTACTGCTCAACGCC 
GGGGTCAATTTCGCCATCATCCTCGCCTTGTTCCTCGGCTTTCTCGCACTCAGCGGGCGCCTGCCGGGCGCG 
GCATTGCTCGCGCTGGTGCCGCTGCTGGCGATCCAGGTGCTGTTCGCGGCGGGACTGGGGATGATCCTCGGG 
GTGCTCAACGTGTTCTTCCGCGACGTCGGCCAGTTGTTCGGCATCTGTCTGCAGTTCTGGTTCTGGCTGACG 
CCGATCGTCTATCCGATCGGCATCCTGCCCGAGGGTATCCGCTCGCTGATCGAACTCAACCCGATGACCGCG 
CTGATGCGCAGCTACCAGCAGCTGTTCCTCCACGGGCAATGGCCGGACTGGCCTTCATTGCTGCCGATCACC 
CTGCTGGCGCTGCTGCTGTGCGCGCTCGGCCTGCGTCTGTTCCGCCAGCGGGTCGGCGAAATGGTGGATGAA 
CTCTGA