ViewGene
Locus Name:
GeneID:
Location:

GeneID:299
TrivialName: PA14_70470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70470
PAO1 Ortholog Locus:PA5338
Sequence Length:2106
Protein Length:701
Start: 6279150
Stop: 6281255
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08723.1|AE004946_7 (AE004946) guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Pseudomonas aeruginosa PAO1]
Identities = 701/701 (100%), Positives = 701/701 (100%)
Sequence:
TTGCCGGGCATAGACGCCTTCGCCGACAGACTGTCGAGCTACCTCGGGCCGGACCAGGTCAACCTGGTCCGC
CGCGCCTACTACTACGCCGAGCAGGCCCACGACGGGCAACGCCGCCGCAGCGGCGAGCCGTACGTGACCCAC
CCGCTGGCGGTCTCCAACATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCCGCGATGCTGCAC
GACGTGATCGAGGACACCGGCATCGCCAAGGAAGCGCTCACCGCGCAGTTCGGCGAGACGGTTTCGGAACTG
GTCGACGGGGTCAGCAAGCTGACCCAGATGAACTTCGAGACCAAGGCCGAGGCCCAGGCCGAGAACTTCCAG
AAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATG
CGCACCCTCGAGGTGCTGTCCGGCGAGAAGCGCCGGCGCATCGCCAAGGAAACCCTGGAAATCTACGCGCCC
ATCGCCAACCGCCTGGGCATGCACACCATGCGCGTGGAGTTCGAGGACCTCGGTTTCAAGGCCATGCACCCG
ATGCGCGCCGAGCGCATCCGCCAGGCGGTGAAGAAGGCCCGCGGCAACCGCCGCGAGATCGTCGGCAAGATC
CAGGAGTCGCTGGTCAACTGCCTCGCCCGCGAAGGCATGGAAGGCCAGGTGATCGGCCGCGAGAAGCACCTC
TACAGCATCTACCAGAAGATGCGCGGCAAGCGTAAGGCGTTCAACGAGATCATGGACGTCTACGCCTTCCGC
ATCATCGTCGACAAGGTCGATACCTGCTATCGCGTACTGGGCGCCGTGCACAGCCTGTACAAGCCGTTCCCC
GGACGCTTCAAGGACTACATCGCGATTCCCAAGGCCAACGGCTACCAGTCGCTGCATACCACCCTGTTCGGC
ATGCACGGGGTGCCCATCGAGATCCAGATCCGTACCCGCGAGATGGAGGAGATGGCCAACCACGGGATCGCC
GCGCACTGGCTGTACAAGTCCAACGACGAGACGCCCAAGGGCACCCATGCCCGCGCCCGGCAATGGGTGAAG
GGCGTGCTGGAACTGCAGCAGCGCGCCGGCAACTCGCTGGAATTCATCGAGAACGTGAAGATCGACCTGTTC
CCGGACGAGGTCTACGTGTTCACGCCCAAGGGCCGCATCATGGAACTGCCCAAGGGTTCCACCGCGGTCGAC
TTCGCCTACGCGGTGCACACCGACGTCGGCAACAGTTGCATCGCCTGCCGCATCAACCGCCGCCTGGCGCCG
CTCTCCGAGCCGTTGCAGAGCGGCCAGACGGTGGAAATCGTCACCGCCCCGGGCGCCCGGCCGAACCCGGCC
TGGCTGAGCTTCGTGGTCACCGGCAAGGCGCGCACCCATATCCGCCATGCGCTCAAGCTGCAACGCCGCTCC
GAGTCGATCAACCTCGGCGAGCGCCTGCTGAACAAGACCCTCACCGGCTTCGACAGCCACCTCGAGAAGATT
CCCCAGGAACGCATCCAGGCCGTGCTCGCCGAGTACCGCATGGAAGTCTTCGAGGACCTGCTGGAAGAGATC
GGCCTGGGCAACCGCATGGCCTACGTGGTCGCCCGCCGCCTGCTGTCCAGCGAGGGCGAGGAAGCGCCGAGC
GCCGAGGGTCCGCTGGCGATCCGCGGCACCGAAGGCCTGGTCCTGAGCTACGCCAAGTGCTGCACGCCGATT
CCCGGCGACCCGATCGTCGGCCACCTGTCCGCCGGCAAGGGCATGGTGGTGCACTTGGAAAGCTGCAAGAAC
ATCAGCGAAGTACGCCACAACCCGGAAAAATGCATCCAGCTCAGCTGGGCCAAGGATGTCACCGGCGAATTC
AATGTCGAACTGCGCGTCGAGCTGGAGCACCAGCGTGGCCTGATCGCCCTGCTCGCCACCAGCGTCAACGCG
GCCGACGGCAACATCGAGAAGATCAGCATGGACGAGCGCGACGGTCGCATCAGCGTGGTCCAGCTGGTCGTC
AGCGTGCACGATCGCGTCCACCTGGCCCGCGTCATCAAGAAGCTGCGCGCCCTCAAGGGAGTGATCCGGATC
ACCCGCCTGCGCAGCTGA
Translation:
MPGIDAFADRLSSYLGPDQVNLVRRAYYYAEQAHDGQRRRSGEPYVTHPLAVSNILADMHMDHQSLMAAMLH
DVIEDTGIAKEALTAQFGETVSELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNM
RTLEVLSGEKRRRIAKETLEIYAPIANRLGMHTMRVEFEDLGFKAMHPMRAERIRQAVKKARGNRREIVGKI
QESLVNCLAREGMEGQVIGREKHLYSIYQKMRGKRKAFNEIMDVYAFRIIVDKVDTCYRVLGAVHSLYKPFP
GRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANHGIAAHWLYKSNDETPKGTHARARQWVK
GVLELQQRAGNSLEFIENVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAP
LSEPLQSGQTVEIVTAPGARPNPAWLSFVVTGKARTHIRHALKLQRRSESINLGERLLNKTLTGFDSHLEKI
PQERIQAVLAEYRMEVFEDLLEEIGLGNRMAYVVARRLLSSEGEEAPSAEGPLAIRGTEGLVLSYAKCCTPI
PGDPIVGHLSAGKGMVVHLESCKNISEVRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLATSVNA
ADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALKGVIRITRLRS*
AnnotationID:309GeneID:299
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 19:07:04
Gene Name:spoT
Confidence Code:2
GeneProduct:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
Cell Localization:()
Synonyms:(p)ppGpp synthetase II
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0317,pfam04607
Secondary Functional Category(ies):signal transduction mechanism
EC Number:3.1.7.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70867.1|AE016731_2 (AE016794) guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
           [Pseudomonas putida KT2440]
          Length = 702

 Score = 1271 bits (3289), Expect = 0.0
 Identities = 631/702 (89%), Positives = 678/702 (96%), Gaps = 1/702 (0%)

>gb|AAO53627.1| (AE016856) guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 701

 Score = 1256 bits (3251), Expect = 0.0
 Identities = 631/702 (89%), Positives = 671/702 (95%), Gaps = 2/702 (0%)

>gb|AAO89860.1| (AE016960) guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase
           [Coxiella burnetii RSA 493]
          Length = 707

 Score =  811 bits (2094), Expect = 0.0
 Identities = 397/692 (57%), Positives = 530/692 (76%), Gaps = 7/692 (1%)








 
Structural Features:
COG0317, SpoT, Guanosine polyphosphate 
pyrophosphohydrolases/synthetases 
[Signal transduction mechanisms / Transcription]
CD-Length = 701 residues,  99.3% aligned
Score =  876 bits (2265), Expect = 0.0
Identities = 300/707 (42%), Positives = 444/707 (62%), Gaps = 19/707 (2%)

pfam04607, RelA_SpoT, Region found in RelA / SpoT proteins.
CD-Length = 113 residues,  97.3% aligned
Score =  175 bits (445), Expect = 2e-44
Identities = 70/110 (63%), Positives = 79/110 (71%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14107




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17743




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34774




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:299_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:299
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
489
                           PA5338_tr
                           translation of PA5338
                           701
                           99.99
                           99.99
                           0
                           3582.0
     

     
     
24879
                           gb|AAG08723.1|AE004946_7
                           (AE004946) guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Pseudomonas aeruginosa PAO1]
                           701
                           99.85
                           99.99
                           0
                           3580.0
     

     
     
24880
                           gb|AAN70867.1|AE016731_2
                           (AE016794) guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Pseudomonas putida KT2440]
                           702
                           89.74
                           96.58
                           1
                           3288.0
     

     
     
24881
                           gb|AAO53627.1|
                           (AE016856) guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           702
                           89.74
                           95.58
                           2
                           3252.0
     

     
     
24883
                           gb|AAN53444.1|AE015484_2
                           (AE015484) guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Shewanella oneidensis MR-1]
                           706
                           56.09
                           74.78
                           10
                           2069.0
     

     






      

          
GC ORFID: 43301How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5338Glimmer Score: 

          
Start: 5542839Stop: 5544944

          
Length: 2106


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5338 translation of PA5338Homolog Bit Score 1384.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3942 (ORF: BLASTX 1260.0), t_PA5338 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGCCGGGCATAGACGCCTTCGCCGACAGACTGTCGAGCTACCTCGGGCCGGACCAGGTCAACCTGGTCCGC 
CGCGCCTACTACTACGCCGAGCAGGCCCACGACGGGCAACGCCGCCGCAGCGGCGAGCCGTACGTGACCCAC 
CCGCTGGCGGTCTCCAACATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCCGCGATGCTGCAC 
GACGTGATCGAGGACACCGGCATCGCCAAGGAAGCGCTCACCGCGCAGTTCGGCGAGACGGTTTCGGAACTG 
GTCGACGGGGTCAGCAAGCTGACCCAGATGAACTTCGAGACCAAGGCCGAGGCCCAGGCCGAGAACTTCCAG 
AAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATG 
CGCACCCTCGAGGTGCTGTCCGGCGAGAAGCGCCGGCGCATCGCCAAGGAAACCCTGGAAATCTACGCGCCC 
ATCGCCAACCGCCTGGGCATGCACACCATGCGCGTGGAGTTCGAGGACCTCGGTTTCAAGGCCATGCACCCG 
ATGCGCGCCGAGCGCATCCGCCAGGCGGTGAAGAAGGCCCGCGGCAACCGCCGCGAGATCGTCGGCAAGATC 
CAGGAGTCGCTGGTCAACTGCCTCGCCCGCGAAGGCATGGAAGGCCAGGTGATCGGCCGCGAGAAGCACCTC 
TACAGCATCTACCAGAAGATGCGCGGCAAGCGTAAGGCGTTCAACGAGATCATGGACGTCTACGCCTTCCGC 
ATCATCGTCGACAAGGTCGATACCTGCTATCGCGTACTGGGCGCCGTGCACAGCCTGTACAAGCCGTTCCCC 
GGACGCTTCAAGGACTACATCGCGATTCCCAAGGCCAACGGCTACCAGTCGCTGCATACCACCCTGTTCGGC 
ATGCACGGGGTGCCCATCGAGATCCAGATCCGTACCCGCGAGATGGAGGAGATGGCCAACCACGGGATCGCC 
GCGCACTGGCTGTACAAGTCCAACGACGAGACGCCCAAGGGCACCCATGCCCGCGCCCGGCAATGGGTGAAG 
GGCGTGCTGGAACTGCAGCAGCGCGCCGGCAACTCGCTGGAATTCATCGAGAACGTGAAGATCGACCTGTTC 
CCGGACGAGGTCTACGTGTTCACGCCCAAGGGCCGCATCATGGAACTGCCCAAGGGTTCCACCGCGGTCGAC 
TTCGCCTACGCGGTGCACACCGACGTCGGCAACAGTTGCATCGCCTGCCGCATCAACCGCCGCCTGGCGCCG 
CTCTCCGAGCCGTTGCAGAGCGGCCAGACGGTGGAAATCGTCACCGCCCCGGGCGCCCGGCCGAACCCGGCC 
TGGCTGAGCTTCGTGGTCACCGGCAAGGCGCGCACCCATATCCGCCATGCGCTCAAGCTGCAACGCCGCTCC 
GAGTCGATCAACCTCGGCGAGCGCCTGCTGAACAAGACCCTCACCGGCTTCGACAGCCACCTCGAGAAGATT 
CCCCAGGAACGCATCCAGGCCGTGCTCGCCGAGTACCGCATGGAAGTCTTCGAGGACCTGCTGGAAGAGATC 
GGCCTGGGCAACCGCATGGCCTACGTGGTCGCCCGCCGCCTGCTGTCCAGCGAGGGCGAGGAAGCGCCGAGC 
GCCGAGGGTCCGCTGGCGATCCGCGGCACCGAAGGCCTGGTCCTGAGCTACGCCAAGTGCTGCACGCCGATT 
CCCGGCGACCCGATCGTCGGCCACCTGTCCGCCGGCAAGGGCATGGTGGTGCACTTGGAAAGCTGCAAGAAC 
ATCAGCGAAGTACGCCACAACCCGGAAAAATGCATCCAGCTCAGCTGGGCCAAGGATGTCACCGGCGAATTC 
AATGTCGAACTGCGCGTCGAGCTGGAGCACCAGCGTGGCCTGATCGCCCTGCTCGCCACCAGCGTCAACGCG 
GCCGACGGCAACATCGAGAAGATCAGCATGGACGAGCGCGACGGTCGCATCAGCGTGGTCCAGCTGGTCGTC 
AGCGTGCACGATCGCGTCCACCTGGCCCGCGTCATCAAGAAGCTGCGCGCCCTCAAGGGAGTGATCCGGATC 
ACCCGCCTGCGCAGCTGA