ViewGene
Locus Name:
GeneID:
Location:

GeneID:2980
TrivialName: PA14_10640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10640
PAO1 Ortholog Locus:PA4122
Sequence Length:780
Protein Length:259
Start: 917619
Stop: 916840
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07509.1|AE004828_10 (AE004828) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 257/259 (99%), Positives = 259/259 (100%)
Sequence:
ATGAAACACGCACGCATCCGCTACCAGGGCCGGGTTCACCAGGTCACCGTGGAAGACGCCAACGCCGTCCGC
CTGGCCGAAGGCACCCTGCTCGCCGAGGACCGGGTGGAGTGGCTGCCGCCGGCCACCGGGAGCATGTTCGCC
CTCGGCCTGAACTACGCCGACCATGCCGCCGAACTGTCGTTCAAGGCGCCCAGCGAACCGCTGGCGTTCATC
AAGTCGCCGGGCACCTACACCGGCCACCGCCAGGTCAGCTGGCGTCCGGACAACGTCGACTACATGCACTAC
GAGTGCGAACTGGTGGCAGTGATCGGCAAGCCGGCGCGCAACGTCCGCCGCGAGGACGCCCTCGGCTACCTG
GCCGGCTACACGGTGTGCAACGACTACGCGATCCGCGACTACCTGGAGAACTACTACCGGCCCAACCTGCGG
GTGAAGAACCGCGACGCCACCACCCCGGTGGGGCCGTGGATCGTCGACGCGGCCGAGGTTCCCGAGCCGAAC
CGGCTGACCCTGCGCACCTGGGTCAACGGCGAGCTGCGCCAGGAAGGCAGCACCGCGGACATGATCTTCGAC
ATTCCCTACCTGATCGAATACTTCTCCAGCTTCATGACCCTGCAGCCGGGCGACATGATCGCCACCGGCACC
CCGGAAGGCCTGTCCGACGTGGTCCCCGGCGACGAAGTGGTGGTGGAGGTGGAAGGCGTCGGTCGCCTGGTC
AACCGAATCGTCAGCGAGGCCGAATTCTTCCGCGCCCGCGCACAGGAGCACGCAGAATGA
Translation:
MKHARIRYQGRVHQVTVEDANAVRLAEGTLLAEDRVEWLPPATGSMFALGLNYADHAAELSFKAPSEPLAFI
KSPGTYTGHRQVSWRPDNVDYMHYECELVAVIGKPARNVRREDALGYLAGYTVCNDYAIRDYLENYYRPNLR
VKNRDATTPVGPWIVDAAEVPEPNRLTLRTWVNGELRQEGSTADMIFDIPYLIEYFSSFMTLQPGDMIATGT
PEGLSDVVPGDEVVVEVEGVGRLVNRIVSEAEFFRARAQEHAE*
AnnotationID:2988GeneID:2980
AnnotatorUID: diggins
Modification Date/Time: 2005-06-07 10:10:24
Gene Name:
Confidence Code:3
GeneProduct:putative 4-hydroxyphenylacetate degradation bifunctional isomerase
Cell Localization:()
Synonyms:hpaG2
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:putative fumarylacetoacetate hydrolase family protein
Functional Category Confidence Code:5
COGs:COG0179,pfam01557
Secondary Functional Category(ies):carbon compound metabolism
EC Number:
Status:ACTIVE
Pathway:
4-hydroxyphenylacetate catabolism.
Homology:
>gb|AAO17178.1| (AF346500) HpaG2 [Photorhabdus luminescens]
          Length = 253

 Score =  371 bits (953), Expect = e-102
 Identities = 175/249 (70%), Positives = 205/249 (82%)

>gb|AAL20033.1| (AE008747) 4-hydroxyphenylacetate catabolism protein [Salmonella
           typhimurium LT2]
          Length = 429

 Score =  331 bits (849), Expect = 2e-90
 Identities = 160/222 (72%), Positives = 181/222 (81%)

>gb|AAO69439.1| (AE016840) 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase;
           2-hydroxyhepta-2,4-diene-1,7-dioate decarboxylase
           [Salmonella enterica subsp. enterica serovar Typhi Ty2]
          Length = 429

 Score =  331 bits (849), Expect = 2e-90
 Identities = 160/222 (72%), Positives = 181/222 (81%)
Structural Features:
COG0179, MhpD, 2-keto-4-pentenoate hydratase/
2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 266 residues,  85.3% aligned
Score =  271 bits (693), Expect = 8e-74
Identities = 106/227 (46%), Positives = 129/227 (56%), Gaps = 3/227 (1%)

pfam01557, FAA_hydrolase, Fumarylacetoacetate (FAA) hydrolase family.
CD-Length = 168 residues, 100.0% aligned
Score =  188 bits (478), Expect = 8e-49
Identities = 78/168 (46%), Positives = 98/168 (58%), Gaps = 2/168 (1%)
Genomic Context:






   
Comment:


















    ReferenceID:5963




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23105




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36855




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2980_cdd.html


    		




 
URL:

















    ReferenceID:36958




  
Author/Investigator(s):
        Prieto MA, Diaz E, Garcia JL.





  
Title:
        Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.




  
PubMed:
        8550403


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):111-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550403









           
Homologs By Global Alignment 

           

       



     
Gene ID:2980
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4825
                           PA4122_tr
                           translation of PA4122
                           259
                           99.22
                           99.99
                           0
                           1359.0
     

     
     
137654
                           gb|AAG07509.1|AE004828_10
                           (AE004828) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           259
                           99.22
                           99.99
                           0
                           1359.0
     

     
     
137655
                           emb|CAE13284.1|
                           (BX571862) hpaG2 [Photorhabdus luminescens subsp. laumondii TTO1]
                           259
                           67.95
                           79.92
                           6
                           955.0
     

     
     
137656
                           gb|AAO17178.1|
                           (AF346500) HpaG2 [Photorhabdus luminescens]
                           259
                           67.56
                           79.53
                           6
                           949.0
     

     
     
137657
                           gb|AAK03613.1|
                           (AE006189) HpaG [Pasteurella multocida]
                           260
                           64.61
                           76.92
                           7
                           905.0
     

     






      

          
GC ORFID: 45982How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4122Glimmer Score: 

          
Start: 4036318Stop: 4037097

          
Length: 780


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4122 translation of PA4122Homolog Bit Score 528.0

          
Other Homologs:  t_PA4121 (ORF: BLASTX 161.0), t_PA4122 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACACGCACGCATCCGCTACCAGGGCCGGGTTCACCAGGTCACCGTGGAAGACGCCAACGCCGTCCGC 
CTGGCCGAAGGCACCCTGCTCGCCGAGGACCGGGTGGAGTGGCTGCCGCCGGCCACCGGGAGCATGTTCGCC 
CTCGGCCTGAACTACGCCGACCATGCCGCCGAACTGTCGTTCAAGGCGCCCAGCGAACCGCTGGCGTTCATC 
AAGTCGCCGGGCACCTACACCGGCCACCGCCAGGTCAGCTGGCGTCCGGACAACGTCGACTACATGCACTAC 
GAGTGCGAACTGGTGGCAGTGATCGGCAAGCCGGCGCGCAACGTCCGCCGCGAGGACGCCCTCGGCTACCTG 
GCCGGCTACACGGTGTGCAACGACTACGCGATCCGCGACTACCTGGAGAACTACTACCGGCCCAACCTGCGG 
GTGAAGAACCGCGACGCCACCACCCCGGTGGGGCCGTGGATCGTCGACGCGGCCGAGGTTCCCGAGCCGAAC 
CGGCTGACCCTGCGCACCTGGGTCAACGGCGAGCTGCGCCAGGAAGGCAGCACCGCGGACATGATCTTCGAC 
ATTCCCTACCTGATCGAATACTTCTCCAGCTTCATGACCCTGCAGCCGGGCGACATGATCGCCACCGGCACC 
CCGGAAGGCCTGTCCGACGTGGTCCCCGGCGACGAAGTGGTGGTGGAGGTGGAAGGCGTCGGTCGCCTGGTC 
AACCGAATCGTCAGCGAGGCCGAATTCTTCCGCGCCCGCGCACAGGAGCACGCAGAATGA