ViewGene
Locus Name:
GeneID:
Location:

GeneID:2970
TrivialName: PA14_10590
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10590
PAO1 Ortholog Locus:PA4127
Sequence Length:804
Protein Length:267
Start: 912510
Stop: 911707
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07514.1|AE004829_4 (AE004829) 2-oxo-hept-3-ene-1,7-dioate hydratase [Pseudomonas aeruginosa PAO1]
Identities = 267/267 (100%), Positives = 267/267 (100%)
Sequence:
ATGCTCGATCCCCGCCTGATCCAGGAAGCCGCCAACCGCCTCGACGCCGCCGAACGCTCGCGCCAGCAGGTA
AGGCAGTTCTCCCTCGACTACCCGGACATCGCCATCGAGGACGCCTACGCCATCCAGCGCGCCTGGGTCGAG
CGCAAGATCGCCGACGGGCGCGTACTCAAGGGCCACAAGATCGGCCTGACCTCGCGCGCCATGCAGGTCTCG
TCGAACATCAGCGAGCCGGACTACGGCGCCTTGCTCGACGACATGTTCTTCGAGGAAGGCAGCGACATCCCC
TTCCAGCGCTTCATCGTGCCGCGGGTCGAGGTGGAGCTGGCGTTCATCCTCGGCAAGCCGCTGAAGGGACCG
GGCTGCACCCTGTTCGACGTGCTCGAGGCCACCGAGTGGGTGATCCCGGCGCTGGAAATCATCGATGCGCGC
ATCCAGCAGGTCGACCCGCAAACGAAAGTCACGCGCAAGGTCTTCGACACCATCTCCGACAACGCCGCCAAC
GCCGGCGTGGTGATGGGCGGCCGGGCCGTGCGGCCAGGCGATATCGACCTGCGGCGGGTACCGGCGATCCTC
TACCGCAATGGCGTGATCGAGGAGTCTGGCGTCTCCGCCGCGGTGCTCAACCACCCGGCCAAGGGCGTCGCC
TGGCTGGCCAACAAGCTGGCGCCCTACGGCGTCTGCCTGGAGGCCGGCCAGGTGATCCTCGGCGGCTCCTTC
ACCCGCCCGGTGGCGGCCGCGCCGGGGGACACCTTCCACGTCGACTACGATCTGCTCGGCAGTATCTCCTGC
CGTTTCGTCTGA
Translation:
MLDPRLIQEAANRLDAAERSRQQVRQFSLDYPDIAIEDAYAIQRAWVERKIADGRVLKGHKIGLTSRAMQVS
SNISEPDYGALLDDMFFEEGSDIPFQRFIVPRVEVELAFILGKPLKGPGCTLFDVLEATEWVIPALEIIDAR
IQQVDPQTKVTRKVFDTISDNAANAGVVMGGRAVRPGDIDLRRVPAILYRNGVIEESGVSAAVLNHPAKGVA
WLANKLAPYGVCLEAGQVILGGSFTRPVAAAPGDTFHVDYDLLGSISCRFV*
AnnotationID:2978GeneID:2970
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 13:01:22
Gene Name:hpcG
Confidence Code:2
GeneProduct:2-oxo-hept-3-ene-1,7-dioate hydratase
Cell Localization:()
Synonyms:hpaH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:4-hydroxyphenylacetate catabolism protein
Functional Category Confidence Code:5
COGs:COG3971,pfam01689
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:4.2.-.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK03618.1| (AE006190) HpaH [Pasteurella multocida]
          Length = 267

 Score =  407 bits (1046), Expect = e-113
 Identities = 204/267 (76%), Positives = 233/267 (87%)

>emb|CAA86044.1| (Z37980) 2-oxo-hept-3-ene-1,7-dioate hydratase [Escherichia coli]
          Length = 267

 Score =  397 bits (1021), Expect = e-110
 Identities = 192/267 (71%), Positives = 222/267 (83%)

>gb|AAL20037.1| (AE008748) 4-hydroxyphenylacetate catabolism protein [Salmonella
           typhimurium LT2]
          Length = 267

 Score =  393 bits (1010), Expect = e-109
 Identities = 191/267 (71%), Positives = 223/267 (83%)






 
Structural Features:
COG3971, COG3971, 2-keto-4-pentenoate hydratase 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 264 residues,  99.2% aligned
Score =  367 bits (943), Expect = 9e-103
Identities = 168/267 (62%), Positives = 197/267 (73%), Gaps = 5/267 (1%)

pfam01689, Hyratase_decarb, Hydratase/decarboxylase.
CD-Length = 219 residues,  99.5% aligned
Score =  279 bits (714), Expect = 3e-76
Identities = 105/224 (46%), Positives = 141/224 (62%), Gaps = 6/224 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5943




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23085




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36885




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2970_cdd.html


    		




 
URL:

















    ReferenceID:36960




  
Author/Investigator(s):
        Prieto MA, Diaz E, Garcia JL.





  
Title:
        Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.




  
PubMed:
        8550403


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):111-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550403

















    ReferenceID:36966




  
Author/Investigator(s):
        Roper DI, Stringfellow JM, Cooper RA.





  
Title:
        Sequence of the hpcC and hpcG genes of the meta-fission homoprotocatechuic acid pathway of Escherichia coli C: nearly 40% amino-acid identity with the analogous enzymes of the catechol pathway.




  
PubMed:
        7737515


MedLine:
        




    
Source:
Gene. 1995 Apr 14;156(1):47-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7737515

















    ReferenceID:36974




  
Author/Investigator(s):
        Jenkins JR, Cooper RA.





  
Title:
        Molecular cloning, expression, and analysis of the genes of the homoprotocatechuate catabolic pathway of Escherichia coli C.




  
PubMed:
        3053656


MedLine:
        




    
Source:
J Bacteriol. 1988 Nov;170(11):5317-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3053656









           
Homologs By Global Alignment 

           

       



     
Gene ID:2970
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4813
                           PA4127_tr
                           translation of PA4127
                           267
                           99.99
                           99.99
                           0
                           1364.0
     

     
     
137509
                           gb|AAG07514.1|AE004829_4
                           (AE004829) 2-oxo-hept-3-ene-1,7-dioate hydratase [Pseudomonas aeruginosa PAO1]
                           267
                           99.99
                           99.99
                           0
                           1364.0
     

     
     
137510
                           gb|AAK03618.1|
                           (AE006190) HpaH [Pasteurella multocida]
                           267
                           76.40
                           87.26
                           0
                           1046.0
     

     
     
137512
                           emb|CAE13278.1|
                           (BX571862) 2-oxo-hepta-3-ene-1,7-dioic acid hydratase [Photorhabdus luminescens subsp. laumondii TTO1]
                           267
                           74.15
                           84.64
                           0
                           1040.0
     

     
     
137511
                           gb|AAO17184.1|
                           (AF346500) HpaH [Photorhabdus luminescens]
                           267
                           73.78
                           85.01
                           0
                           1042.0
     

     






      

          
GC ORFID: 45972How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4127Glimmer Score: 

          
Start: 4041427Stop: 4042230

          
Length: 804


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4127 translation of PA4127Homolog Bit Score 530.0

          
Other Homologs:  t_PA4127 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCGATCCCCGCCTGATCCAGGAAGCCGCCAACCGCCTCGACGCCGCCGAACGCTCGCGCCAGCAGGTA 
AGGCAGTTCTCCCTCGACTACCCGGACATCGCCATCGAGGACGCCTACGCCATCCAGCGCGCCTGGGTCGAG 
CGCAAGATCGCCGACGGGCGCGTACTCAAGGGCCACAAGATCGGCCTGACCTCGCGCGCCATGCAGGTCTCG 
TCGAACATCAGCGAGCCGGACTACGGCGCCTTGCTCGACGACATGTTCTTCGAGGAAGGCAGCGACATCCCC 
TTCCAGCGCTTCATCGTGCCGCGGGTCGAGGTGGAGCTGGCGTTCATCCTCGGCAAGCCGCTGAAGGGACCG 
GGCTGCACCCTGTTCGACGTGCTCGAGGCCACCGAGTGGGTGATCCCGGCGCTGGAAATCATCGATGCGCGC 
ATCCAGCAGGTCGACCCGCAAACGAAAGTCACGCGCAAGGTCTTCGACACCATCTCCGACAACGCCGCCAAC 
GCCGGCGTGGTGATGGGCGGCCGGGCCGTGCGGCCAGGCGATATCGACCTGCGGCGGGTACCGGCGATCCTC 
TACCGCAATGGCGTGATCGAGGAGTCTGGCGTCTCCGCCGCGGTGCTCAACCACCCGGCCAAGGGCGTCGCC 
TGGCTGGCCAACAAGCTGGCGCCCTACGGCGTCTGCCTGGAGGCCGGCCAGGTGATCCTCGGCGGCTCCTTC 
ACCCGCCCGGTGGCGGCCGCGCCGGGGGACACCTTCCACGTCGACTACGATCTGCTCGGCAGTATCTCCTGC 
CGTTTCGTCTGA