ViewGene
Locus Name:
GeneID:
Location:

GeneID:2969
TrivialName: PA14_14700
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14700
PAO1 Ortholog Locus:PA3816
Sequence Length:777
Protein Length:258
Start: 1252915
Stop: 1253691
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07203.1|AE004799_9 (AE004799) O-acetylserine synthase [Pseudomonas aeruginosa PAO1]
Identities = 257/258 (99%), Positives = 258/258 (100%)
Sequence:
ATGTTCGAACGTGTGCGTGAAGATATCCAAAGCGTATTCCACCGTGATCCGGCGGCTCGCAACGCACTCGAG
GTCCTCACCTGTTATCCGGGCCTGCACGCCGTCTGGCTGCATCGGCTGGCCCACGGCTTGTGGACGTCCGGC
TGGAAGTGGCTGGCGCGCCTGGTGTCCAATTTCGGCCGCTGGATGACCGGCATCGAGATCCACCCGGGGGCC
AGGATCGGCCGGCGCTTCTTCATCGACCACGGCATGGGCATCGTCATCGGCGAAACCGCCGAGATCGGCGAC
GACGTCACCCTCTACCAGGGCGTCACCCTGGGCGGTACCACCTGGAACAAGGGCAAGCGCCACCCCACCCTC
GGCAACAACGTGGTGGTCGGTGCCGGTGCCAAGGTGCTCGGCCCGTTCACCGTCGGCGAGGGCGCCAAGGTC
GGCTCCAATGCCGTGGTGACCAAGGAAGTCCCGCCGGGTGCCACCGTGGTCGGCATTCCCGGGCGGATCATC
ATGCGCGAGGATACCGAGCAGCAGGCCAAGCGCCAGGCGATGGCGGAGAAACTCGGCTTCGATGCCTACGGA
GTCAGTCAGGACATGCCGGACCCGGTGGCGCGTGCCATTGGTCAGTTGCTCGATCACCTGCAGGCCGTCGAC
GGCCGGCTCGAGGGCATGTGCCAGGCGCTCACCGCCCTGGGGAGCGACTACTGCGCGAAGGATCTGCCGGTA
TTGCGCGAGGAGGATTTCGCCGGGGTCAAGGACGAGGATGGCAATCCGGCCGCGTAA
Translation:
MFERVREDIQSVFHRDPAARNALEVLTCYPGLHAVWLHRLAHGLWTSGWKWLARLVSNFGRWMTGIEIHPGA
RIGRRFFIDHGMGIVIGETAEIGDDVTLYQGVTLGGTTWNKGKRHPTLGNNVVVGAGAKVLGPFTVGEGAKV
GSNAVVTKEVPPGATVVGIPGRIIMREDTEQQAKRQAMAEKLGFDAYGVSQDMPDPVARAIGQLLDHLQAVD
GRLEGMCQALTALGSDYCAKDLPVLREEDFAGVKDEDGNPAA*
AnnotationID:2977GeneID:2969
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 12:12:15
Gene Name:cysE
Confidence Code:2
GeneProduct:serine O-acetyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1045
Secondary Functional Category(ies):
EC Number:2.3.1.30
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54942.1| (AE016860) serine O-acetyltransferase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 258

 Score =  440 bits (1131), Expect = e-123
 Identities = 209/251 (83%), Positives = 227/251 (90%)

>dbj|BAB81027.1| (AP003190) serine O-acetyltransferase [Clostridium perfringens str.
           13]
          Length = 169

 Score =  219 bits (558), Expect = 1e-56
 Identities = 102/167 (61%), Positives = 131/167 (78%)








 
Structural Features:
COG1045, CysE, Serine acetyltransferase 
[Amino acid transport and metabolism]
CD-Length = 194 residues,  92.3% aligned
Score =  265 bits (680), Expect = 5e-72
Identities = 109/179 (60%), Positives = 135/179 (75%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5941




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23083




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31740




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2969_cdd.html


    		




 
URL:

















    ReferenceID:34302




  
Author/Investigator(s):
        Denk D, Bock A.





  
Title:
        L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant.




  
PubMed:
        3309158


MedLine:
        




    
Source:
J Gen Microbiol. 1987 Mar;133 ( Pt 3):515-25.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3309158

















    ReferenceID:34303




  
Author/Investigator(s):
        Lai CY, Baumann P.





  
Title:
        Sequence analysis of a DNA fragment from Buchnera aphidicola (an endosymbiont of aphids) containing genes homologous to dnaG, rpoD, cysE, and secB.




  
PubMed:
        1398077


MedLine:
        




    
Source:
Gene. 1992 Sep 21;119(1):113-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1398077









           
Homologs By Global Alignment 

           

       



     
Gene ID:2969
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4811
                           PA3816_tr
                           translation of PA3816
                           258
                           99.61
                           99.99
                           0
                           1365.0
     

     
     
137384
                           gb|AAG07203.1|AE004799_9
                           (AE004799) O-acetylserine synthase [Pseudomonas aeruginosa PAO1]
                           258
                           99.61
                           99.99
                           0
                           1365.0
     

     
     
137386
                           gb|AAN66465.1|AE016275_10
                           (AE016777) serine O-acetyltransferase [Pseudomonas putida KT2440]
                           262
                           81.29
                           87.78
                           5
                           1120.0
     

     
     
137385
                           gb|AAO54942.1|
                           (AE016860) serine O-acetyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           260
                           81.15
                           88.07
                           4
                           1125.0
     

     
     
137387
                           gb|AAC24481.1|
                           (AF010139) O-acetylserine synthase; CysE2 [Azotobacter vinelandii]
                           258
                           77.90
                           86.43
                           7
                           1075.0
     

     






      

          
GC ORFID: 45971How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3816Glimmer Score: 

          
Start: 3701023Stop: 3700247

          
Length: 777


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3816 translation of PA3816Homolog Bit Score 530.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4515 (ORF: BLASTX 438.0), t_PA3816 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTCGAACGTGTGCGTGAAGATATCCAAAGCGTATTCCACCGTGATCCGGCGGCTCGCAACGCACTCGAG 
GTCCTCACCTGTTATCCGGGCCTGCACGCCGTCTGGCTGCATCGGCTGGCCCACGGCTTGTGGACGTCCGGC 
TGGAAGTGGCTGGCGCGCCTGGTGTCCAATTTCGGCCGCTGGATGACCGGCATCGAGATCCACCCGGGGGCC 
AGGATCGGCCGGCGCTTCTTCATCGACCACGGCATGGGCATCGTCATCGGCGAAACCGCCGAGATCGGCGAC 
GACGTCACCCTCTACCAGGGCGTCACCCTGGGCGGTACCACCTGGAACAAGGGCAAGCGCCACCCCACCCTC 
GGCAACAACGTGGTGGTCGGTGCCGGTGCCAAGGTGCTCGGCCCGTTCACCGTCGGCGAGGGCGCCAAGGTC 
GGCTCCAATGCCGTGGTGACCAAGGAAGTCCCGCCGGGTGCCACCGTGGTCGGCATTCCCGGGCGGATCATC 
ATGCGCGAGGATACCGAGCAGCAGGCCAAGCGCCAGGCGATGGCGGAGAAACTCGGCTTCGATGCCTACGGA 
GTCAGTCAGGACATGCCGGACCCGGTGGCGCGTGCCATTGGTCAGTTGCTCGATCACCTGCAGGCCGTCGAC 
GGCCGGCTCGAGGGCATGTGCCAGGCGCTCACCGCCCTGGGGAGCGACTACTGCGCGAAGGATCTGCCGGTA 
TTGCGCGAGGAGGATTTCGCCGGGGTCAAGGACGAGGATGGCAATCCGGCCGCGTAA