ViewGene
Locus Name:
GeneID:
Location:

GeneID:2968
TrivialName: PA14_20300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20300
PAO1 Ortholog Locus:PA3384
Sequence Length:837
Protein Length:278
Start: 1748289
Stop: 1749125
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06772.1|AE004759_19 (AE004759) ATP-binding component of ABC phosphonate transporter [Pseudomonas aeruginosa PAO1]
Identities = 276/278 (99%), Positives = 278/278 (100%)
Sequence:
ATGAGCGCGGTGATCCGTGTCGACAGCCTGAACAAGACCTTTGCCCGCAAGCAGGCGCTTTTCAACCTGAAG
CTGGAGATCCAGGCGGGCGAGATGGTGGCGTTGATCGGTGCATCCGGCTCCGGCAAATCCACGCTCCTGCGT
CACGTCGCCGGGCTCGCCCGCTGCGACCGCGACAACGGCGGCAGCATCGACGTGCTGGGTCGCCGCCTGCAG
GCGTCCGGTCGCCTGAGCGGCGAAGTGCGCCGCCTGCGCGCCGACATCGGCTACATCTTCCAGCAGTTCAAC
CTGGTCAACCGCCTCAGCGTGCTGGACAACGTCCTGCTCGGCTTCCTCGGTCGCATGCCGCGCTGGCGCGGT
TCGCTGGGGCTGTTCAGCGCCGAGCAGAAGCGCCAGGCCCTGGAAGCGCTGGCGCGGGTCGGCCTGGCCGAT
TTCGCCGGGCAGCGCGCCTCGACCCTCTCCGGCGGCCAGCAGCAGCGCGTAGCCATCGCCCGCGCCCTGACC
CAGAAGGCCGAGGTGATTCTCGCCGACGAACCGATCGCCTCGCTCGACCCGGAGTCGGCGCGCAAGGTGATG
GACATCCTCGCCGACATCAATCGCCACGACGGCAAGACCGTGGTGGTGACCCTGCATCAGGTCGACTACGCG
TTGCGCTATTGCCCGCGCGCGGTGGCCCTGAAGGGCGGCCGGATCCTCTTCGACGGCAGCAGCGAGCACCTT
TCCGAAGGCTTCCTCAACGAACTCTACGGCGCCGAAGGGGACACCCCGTTGCTGTTTTCCGACCGTGCCCGG
CGCGGGGCGGAGAGCCAGCCGGAACTGACCCTCGCGCGCGCCTGA
Translation:
MSAVIRVDSLNKTFARKQALFNLKLEIQAGEMVALIGASGSGKSTLLRHVAGLARCDRDNGGSIDVLGRRLQ
ASGRLSGEVRRLRADIGYIFQQFNLVNRLSVLDNVLLGFLGRMPRWRGSLGLFSAEQKRQALEALARVGLAD
FAGQRASTLSGGQQQRVAIARALTQKAEVILADEPIASLDPESARKVMDILADINRHDGKTVVVTLHQVDYA
LRYCPRAVALKGGRILFDGSSEHLSEGFLNELYGAEGDTPLLFSDRARRGAESQPELTLARA*
AnnotationID:2976GeneID:2968
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 08:08:44
Gene Name:phnC
Confidence Code:2
GeneProduct:ATP-binding component of ABC phosphonate transporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:HisP-like nucleotide binding protein (phnC
Functional Category Confidence Code:5
COGs:COG3638,COG1126,COG1136
Secondary Functional Category(ies):
EC Number:3.6.3.28
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56060.1| (AE016865) phosphonates ABC transporter, ATP-binding protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 277

 Score =  409 bits (1050), Expect = e-113
 Identities = 209/277 (75%), Positives = 241/277 (87%), Gaps = 1/277 (0%)

>gb|AAA24339.1| (J05260) HisP-like nucleotide binding protein (phnC) [Escherichia
           coli]
          Length = 262

 Score =  302 bits (773), Expect = 2e-81
 Identities = 158/249 (63%), Positives = 190/249 (76%)

>gb|AAK22348.1| (AE005709) phosphonates ABC transporter, ATP-binding protein
           [Caulobacter crescentus CB15]
          Length = 264

 Score =  263 bits (673), Expect = 6e-70
 Identities = 140/249 (56%), Positives = 182/249 (73%), Gaps = 1/249 (0%)








 
Structural Features:
COG3638, ABC-type phosphate/phosphonate transport system, ATPase component 
[Inorganic ion transport and metabolism]
CD-Length = 258 residues, 100.0% aligned
Score =  326 bits (838), Expect = 1e-90
Identities = 153/269 (56%), Positives = 189/269 (70%), Gaps = 12/269 (4%)

COG1126, GlnQ, ABC-type polar amino acid transport system, ATPase component 
[Amino acid transport and metabolism]
CD-Length = 240 residues,  92.9% aligned
Score =  196 bits (499), Expect = 3e-51
Identities = 95/238 (39%), Positives = 140/238 (58%), Gaps = 15/238 (6%)

COG1136, SalX, ABC-type antimicrobial peptide transport system, 
ATPase component [Defense mechanisms]
CD-Length = 226 residues,  97.3% aligned
Score =  188 bits (479), Expect = 6e-49
Identities = 93/234 (39%), Positives = 145/234 (61%), Gaps = 20/234 (8%)










   
Genomic Context:






   
Comment:


















    ReferenceID:5939




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23081




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32177




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2968_cdd.html


    		




 
URL:

















    ReferenceID:34037




  
Author/Investigator(s):
        Yakovleva GM, Kim SK, Wanner BL.





  
Title:
        Phosphate-independent expression of the carbon-phosphorus lyase activity of Escherichia coli.




  
PubMed:
        9650256


MedLine:
        




    
Source:
Appl Microbiol Biotechnol. 1998 May;49(5):573-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9650256

















    ReferenceID:34038




  
Author/Investigator(s):
        Metcalf WW, Wanner BL.





  
Title:
        Evidence for a fourteen-gene, phnC to phnP locus for phosphonate metabolism in Escherichia coli.




  
PubMed:
        8335257


MedLine:
        




    
Source:
Gene. 1993 Jul 15;129(1):27-32. 	Related Articles, Links







    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8335257

















    ReferenceID:34039




  
Author/Investigator(s):
        Wanner BL, Metcalf WW.





  
Title:
        Molecular genetic studies of a 10.9-kb operon in Escherichia coli for phosphonate uptake and biodegradation.




  
PubMed:
        1335942


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1992 Dec 15;79(1-3):133-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1335942

















    ReferenceID:34040




  
Author/Investigator(s):
        Wanner BL.





  
Title:
        Genes for phosphonate biodegradation in Escherichia coli.




  
PubMed:
        1368181


MedLine:
        




    
Source:
SAAS Bull Biochem Biotechnol. 1992 Jan;5:1-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1368181

















    ReferenceID:34041




  
Author/Investigator(s):
        Makino K, Kim SK, Shinagawa H, Amemura M, Nakata A.





  
Title:
        Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12.




  
PubMed:
        1840580


MedLine:
        




    
Source:
J Bacteriol. 1991 Apr;173(8):2665-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1840580

















    ReferenceID:34042




  
Author/Investigator(s):
        Metcalf WW, Wanner BL.





  
Title:
        Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi.




  
PubMed:
        1846145


MedLine:
        




    
Source:
J Bacteriol. 1991 Jan;173(2):587-600.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1846145

















    ReferenceID:34043




  
Author/Investigator(s):
        Chen CM, Ye QZ, Zhu ZM, Wanner BL, Walsh CT.





  
Title:
        Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B.




  
PubMed:
        2155230


MedLine:
        




    
Source:
J Biol Chem. 1990 Mar 15;265(8):4461-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2155230









           
Homologs By Global Alignment 

           

       



     
Gene ID:2968
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4807
                           PA3384_tr
                           translation of PA3384
                           278
                           99.28
                           99.99
                           0
                           1366.0
     

     
     
137322
                           gb|AAG06772.1|AE004759_19
                           (AE004759) ATP-binding component of ABC phosphonate transporter [Pseudomonas aeruginosa PAO1]
                           278
                           99.28
                           99.99
                           0
                           1366.0
     

     
     
137323
                           gb|AAO56060.1|
                           (AE016865) phosphonates ABC transporter, ATP-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           278
                           75.17
                           86.69
                           1
                           1052.0
     

     
     
137329
                           dbj|BAB38511.1|
                           (AP002568) ATP-binding component of phosphonate transport [Escherichia coli O157:H7]
                           278
                           57.55
                           70.86
                           16
                           765.0
     

     
     
137328
                           dbj|BAA14262.1|
                           (D90227) ATP-binding protein [Escherichia coli]
                           278
                           57.55
                           70.14
                           16
                           765.0
     

     






      

          
GC ORFID: 45970How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3384Glimmer Score: 

          
Start: 3205643Stop: 3204807

          
Length: 837


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3384 translation of PA3384Homolog Bit Score 530.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1216 (ORF: BLASTX 401.0), t_PA4192 (ORF: BLASTX 154.0), t_PA3314 (ORF: BLASTX 152.0), t_PA3384 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCGGTGATCCGTGTCGACAGCCTGAACAAGACCTTTGCCCGCAAGCAGGCGCTTTTCAACCTGAAG 
CTGGAGATCCAGGCGGGCGAGATGGTGGCGTTGATCGGTGCATCCGGCTCCGGCAAATCCACGCTCCTGCGT 
CACGTCGCCGGGCTCGCCCGCTGCGACCGCGACAACGGCGGCAGCATCGACGTGCTGGGTCGCCGCCTGCAG 
GCGTCCGGTCGCCTGAGCGGCGAAGTGCGCCGCCTGCGCGCCGACATCGGCTACATCTTCCAGCAGTTCAAC 
CTGGTCAACCGCCTCAGCGTGCTGGACAACGTCCTGCTCGGCTTCCTCGGTCGCATGCCGCGCTGGCGCGGT 
TCGCTGGGGCTGTTCAGCGCCGAGCAGAAGCGCCAGGCCCTGGAAGCGCTGGCGCGGGTCGGCCTGGCCGAT 
TTCGCCGGGCAGCGCGCCTCGACCCTCTCCGGCGGCCAGCAGCAGCGCGTAGCCATCGCCCGCGCCCTGACC 
CAGAAGGCCGAGGTGATTCTCGCCGACGAACCGATCGCCTCGCTCGACCCGGAGTCGGCGCGCAAGGTGATG 
GACATCCTCGCCGACATCAATCGCCACGACGGCAAGACCGTGGTGGTGACCCTGCATCAGGTCGACTACGCG 
TTGCGCTATTGCCCGCGCGCGGTGGCCCTGAAGGGCGGCCGGATCCTCTTCGACGGCAGCAGCGAGCACCTT 
TCCGAAGGCTTCCTCAACGAACTCTACGGCGCCGAAGGGGACACCCCGTTGCTGTTTTCCGACCGTGCCCGG 
CGCGGGGCGGAGAGCCAGCCGGAACTGACCCTCGCGCGCGCCTGA