ViewGene
Locus Name:
GeneID:
Location:

GeneID:2954
TrivialName: PA14_21640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_21640
PAO1 Ortholog Locus:PA3277
Sequence Length:813
Protein Length:270
Start: 1876590
Stop: 1877402
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06665.1|AE004750_3 (AE004750) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 269/270 (99%), Positives = 270/270 (100%)
Sequence:
ATGCAAAACCGCATGATGATCACCGGTGCCGGCTCCGGGCTGGGGCGCGAAATCGCCCTGCGCTGGGCGCGT
GACGGCTGGCGCCTGGCGCTGGCCGACGTCAACGAAGCCGGCCTGGCGGAAAGCCTGAAACTGGTGCGCGAG
GCGGGTGGCGATGGCTTCACCCAGCGTTGCGACGTACGCGACTACAGTCAACTGACCGCGCTGGCGCAGAGC
TGCGAGGAGAAGTTCGGCGGCATCGACGTCATCGTCAACAACGCCGGGGTCGCCTCCGGCGGTTTCTTCGGC
GAGCTGTCCCTGGAGGACTGGGACTGGCAGATCGCGATCAACCTGATGGGCGTGGTCAAGGGCTGCAAGGCC
TTCCTGCCGCTGCTGGAGCGAAGCAAGGGCAAGATCGTCAACATCGCCTCGATGGCGGCGTTGATGCAGGGC
CCGGCGATGAGCAACTACAACGTGGCCAAGGCCGGGGTGGTGGCGCTTTCGGAAAGCCTGCTGGTAGAGCTG
GCGCTGGTCGAGGTCGGCGTCCATGTGGTCTGTCCTTCGTTCTTCCAGACCAACCTGCTGGACTCTTTCCGC
GGCCCGAGCCCCGAGATGAAGCACCAGGTCGGCAAGTTGCTGGAGAGTTCGCCGATCAGCGCTGCCGACATC
GCCGACTACATCCACGCCGAAGTGGCCAAGGGCAGTTTCCTGATCCTGCCCCACGAGCAGGCGCGGATGGCC
TGGAGGATCAAGCAGCAGAATCCCCAGGCGATCTATGACGAGATGACCGCGATGGCCGGCAAGATGCAGGCC
AAGCGTCAGCGCCAGGCCTGA
Translation:
MQNRMMITGAGSGLGREIALRWARDGWRLALADVNEAGLAESLKLVREAGGDGFTQRCDVRDYSQLTALAQS
CEEKFGGIDVIVNNAGVASGGFFGELSLEDWDWQIAINLMGVVKGCKAFLPLLERSKGKIVNIASMAALMQG
PAMSNYNVAKAGVVALSESLLVELALVEVGVHVVCPSFFQTNLLDSFRGPSPEMKHQVGKLLESSPISAADI
ADYIHAEVAKGSFLILPHEQARMAWRIKQQNPQAIYDEMTAMAGKMQAKRQRQA*
AnnotationID:2962GeneID:2954
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 06:06:35
Gene Name:
Confidence Code:3
GeneProduct:putative oxidoreductase, short chain dehydrogenase/reductase
Cell Localization:()
Synonyms:PA3277
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00106,COG0300,COG1028,COG4221
Secondary Functional Category(ies):putative enzymes
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55147.1| (AE016861) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 271

 Score =  447 bits (1149), Expect = e-125
 Identities = 215/268 (80%), Positives = 244/268 (91%)

>gb|AAN67365.1|AE016362_9 (AE016780) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas putida KT2440]
          Length = 269

 Score =  444 bits (1143), Expect = e-124
 Identities = 218/265 (82%), Positives = 239/265 (90%)






 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase. 
CD-Length = 247 residues,  91.1% aligned
Score =  172 bits (436), Expect = 5e-44
Identities = 77/228 (33%), Positives = 117/228 (51%), Gaps = 5/228 (2%)

COG4221, Short-chain alcohol dehydrogenase of unknown specificity 
CD-Length = 246 residues,  86.6% aligned
Score =  141 bits (356), Expect = 1e-34
Identities = 71/217 (32%), Positives = 105/217 (48%), Gaps = 6/217 (2%)

COG0300, DltE, Short-chain dehydrogenases of various substrate specificities
CD-Length = 265 residues,  98.5% aligned
Score =  140 bits (354), Expect = 2e-34
Identities = 88/270 (32%), Positives = 134/270 (49%), Gaps = 11/270 (4%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  81.3% aligned
Score =  134 bits (338), Expect = 9e-33
Identities = 73/206 (35%), Positives = 109/206 (52%), Gaps = 7/206 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5911




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23053




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32270




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2954_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2954
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4778
                           PA3277_tr
                           translation of PA3277
                           270
                           99.62
                           99.99
                           0
                           1371.0
     

     
     
136740
                           gb|AAG06665.1|AE004750_3
                           (AE004750) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           270
                           99.62
                           99.99
                           0
                           1371.0
     

     
     
136742
                           gb|AAN67365.1|AE016362_9
                           (AE016780) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas putida KT2440]
                           270
                           80.74
                           88.88
                           1
                           1141.0
     

     
     
136741
                           gb|AAO55147.1|
                           (AE016861) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas syringae pv. tomato str. DC3000]
                           271
                           79.33
                           90.03
                           1
                           1145.0
     

     






      

          
GC ORFID: 45956How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3277Glimmer Score: 

          
Start: 3077341Stop: 3076529

          
Length: 813


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3277 translation of PA3277Homolog Bit Score 532.0

          
Other Homologs:  t_PA3277 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAAAACCGCATGATGATCACCGGTGCCGGCTCCGGGCTGGGGCGCGAAATCGCCCTGCGCTGGGCGCGT 
GACGGCTGGCGCCTGGCGCTGGCCGACGTCAACGAAGCCGGCCTGGCGGAAAGCCTGAAACTGGTGCGCGAG 
GCGGGTGGCGATGGCTTCACCCAGCGTTGCGACGTACGCGACTACAGTCAACTGACCGCGCTGGCGCAGAGC 
TGCGAGGAGAAGTTCGGCGGCATCGACGTCATCGTCAACAACGCCGGGGTCGCCTCCGGCGGTTTCTTCGGC 
GAGCTGTCCCTGGAGGACTGGGACTGGCAGATCGCGATCAACCTGATGGGCGTGGTCAAGGGCTGCAAGGCC 
TTCCTGCCGCTGCTGGAGCGAAGCAAGGGCAAGATCGTCAACATCGCCTCGATGGCGGCGTTGATGCAGGGC 
CCGGCGATGAGCAACTACAACGTGGCCAAGGCCGGGGTGGTGGCGCTTTCGGAAAGCCTGCTGGTAGAGCTG 
GCGCTGGTCGAGGTCGGCGTCCATGTGGTCTGTCCTTCGTTCTTCCAGACCAACCTGCTGGACTCTTTCCGC 
GGCCCGAGCCCCGAGATGAAGCACCAGGTCGGCAAGTTGCTGGAGAGTTCGCCGATCAGCGCTGCCGACATC 
GCCGACTACATCCACGCCGAAGTGGCCAAGGGCAGTTTCCTGATCCTGCCCCACGAGCAGGCGCGGATGGCC 
TGGAGGATCAAGCAGCAGAATCCCCAGGCGATCTATGACGAGATGACCGCGATGGCCGGCAAGATGCAGGCC 
AAGCGTCAGCGCCAGGCCTGA