ViewGene
Locus Name:
GeneID:
Location:

GeneID:2949
TrivialName: PA14_02740
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02740
PAO1 Ortholog Locus:PA0224
Sequence Length:783
Protein Length:260
Start: 244787
Stop: 244005
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03613.1|AE004460_6 (AE004460) probable aldolase [Pseudomonas aeruginosa PAO1]
Identities = 260/260 (100%), Positives = 260/260 (100%)
Sequence:
ATGGCTACGACACTGGAATTACCCAAGGATCAACTGATCCAGCACGCGCTGAAGCAGATGAAAAGCAGCCTC
GGCGATAATACGTGGACTGACCGGCAAAAGCTGGCCCTGACCTGCCGGATTCTCTTCGAGGCCGGCCACGAC
TCCGGGCTCGCCGGGCAGATCACCTCGCGCGGCCCGGAGGCGGGGACCTACTACACCCAGCAACTGGGCCTG
GGCTTCGACGAGATCAGCGCCGGCAACCTGCTACTGGTCGACGAAGACCTCAACGTCCTCCACGGGCACGGC
ATGGCGAATCCGGCCAATCGCTTCCACAGCTGGGTCTACCGCGCCCGCCCGGACGTCAATTGCATCATCCAT
ACCCACCCGCTGCACATCGCCGCGCTGTCGACCCTGGAAGTACCGCTGCAGGTCTCGCACATGGACCTTTGC
CCGCTATACGACGACTGCGCCTTCCTCAAGGACTGGCCGGGCGTGCCGGTAGGCAACGAGGAAGGCGAGATC
ATCTCCGCCGCCCTCGGCGACAAGCGCGCCATCCTGCTCTCCCACCACGGCCTGCTGGCCACCGGTGCGAGC
ATCGAGGAGGCCTGCGTGATCGCGGTGCTGCTCGAGCGCGCGGCGAAGATGCAGTTGCTGGCGATGGCCGCT
GGGGAGATCAAGCCGATCCCGCCGGCCCTGGCGCGCGAGGCCCACGACTGGATCTCCACCCCGAAGCGCCAC
GGCGCCGCATTCAACTACTACGCCCGGCGCTGCCTGCCGCGCAACAGCGACTGCCTGAGCTGA
Translation:
MATTLELPKDQLIQHALKQMKSSLGDNTWTDRQKLALTCRILFEAGHDSGLAGQITSRGPEAGTYYTQQLGL
GFDEISAGNLLLVDEDLNVLHGHGMANPANRFHSWVYRARPDVNCIIHTHPLHIAALSTLEVPLQVSHMDLC
PLYDDCAFLKDWPGVPVGNEEGEIISAALGDKRAILLSHHGLLATGASIEEACVIAVLLERAAKMQLLAMAA
GEIKPIPPALAREAHDWISTPKRHGAAFNYYARRCLPRNSDCLS*
AnnotationID:2957GeneID:2949
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 13:01:31
Gene Name:
Confidence Code:3
GeneProduct:putative aldolase
Cell Localization:()
Synonyms:PA0224
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0235,pfam00596,cd00398
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67651.1|AE016395_2 (AE016781) aldolase, putative [Pseudomonas putida KT2440]
          Length = 260

 Score =  430 bits (1106), Expect = e-120
 Identities = 205/259 (79%), Positives = 229/259 (88%)

>emb|CAD83959.1| (BX321856) Class II Aldolase and Adducin N-terminal domain
           [Nitrosomonas europaea ATCC 19718]
          Length = 262

 Score =  355 bits (912), Expect = 1e-97
 Identities = 176/256 (68%), Positives = 202/256 (78%)

>emb|CAE33570.1| (BX640446) putative aldolase [Bordetella bronchiseptica]
          Length = 263

 Score =  280 bits (717), Expect = 5e-75
 Identities = 135/252 (53%), Positives = 179/252 (71%), Gaps = 1/252 (0%)








 
Structural Features:
COG0235, AraD, Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases 
[Carbohydrate transport and metabolism] 
CD-Length = 219 residues,  95.4% aligned
Score =  163 bits (414), Expect = 2e-41
Identities = 67/210 (31%), Positives = 101/210 (48%), Gaps = 3/210 (1%)

pfam00596, Aldolase_II, Class II Aldolase and Adducin N-terminal domain.
CD-Length = 198 residues,  97.0% aligned
Score =  151 bits (383), Expect = 7e-38
Identities = 68/193 (35%), Positives = 102/193 (52%), Gaps = 5/193 (2%)

cd00398, Aldolase_II, Class II Aldolase and Adducin head (N-terminal) domain.
CD-Length = 209 residues,  93.8% aligned
Score =  102 bits (256), Expect = 4e-23
Identities = 60/197 (30%), Positives = 103/197 (52%), Gaps = 4/197 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5901




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23043




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33567




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2949_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2949
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4771
                           PA0224_tr
                           translation of PA0224
                           260
                           99.99
                           99.99
                           0
                           1374.0
     

     
     
136666
                           gb|AAG03613.1|AE004460_6
                           (AE004460) probable aldolase [Pseudomonas aeruginosa PAO1]
                           260
                           99.99
                           99.99
                           0
                           1374.0
     

     
     
136667
                           gb|AAN67651.1|AE016395_2
                           (AE016781) aldolase, putative [Pseudomonas putida KT2440]
                           260
                           78.84
                           88.07
                           0
                           1106.0
     

     
     
136668
                           emb|CAD83959.1|
                           (BX321856) Class II Aldolase and Adducin N-terminal domain [Nitrosomonas europaea ATCC 19718]
                           262
                           67.17
                           77.09
                           2
                           908.0
     

     
     
136669
                           emb|CAC40968.1|
                           (AJ314791) hypothetical protein [Vibrio sp. CH-291]
                           260
                           58.46
                           75.38
                           1
                           827.0
     

     






      

          
GC ORFID: 45951How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0224Glimmer Score: 

          
Start: 6046128Stop: 6045346

          
Length: 783


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0224 translation of PA0224Homolog Bit Score 533.0

          
Other Homologs:  t_PA0224 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTACGACACTGGAATTACCCAAGGATCAACTGATCCAGCACGCGCTGAAGCAGATGAAAAGCAGCCTC 
GGCGATAATACGTGGACTGACCGGCAAAAGCTGGCCCTGACCTGCCGGATTCTCTTCGAGGCCGGCCACGAC 
TCCGGGCTCGCCGGGCAGATCACCTCGCGCGGCCCGGAGGCGGGGACCTACTACACCCAGCAACTGGGCCTG 
GGCTTCGACGAGATCAGCGCCGGCAACCTGCTACTGGTCGACGAAGACCTCAACGTCCTCCACGGGCACGGC 
ATGGCGAATCCGGCCAATCGCTTCCACAGCTGGGTCTACCGCGCCCGCCCGGACGTCAATTGCATCATCCAT 
ACCCACCCGCTGCACATCGCCGCGCTGTCGACCCTGGAAGTACCGCTGCAGGTCTCGCACATGGACCTTTGC 
CCGCTATACGACGACTGCGCCTTCCTCAAGGACTGGCCGGGCGTGCCGGTAGGCAACGAGGAAGGCGAGATC 
ATCTCCGCCGCCCTCGGCGACAAGCGCGCCATCCTGCTCTCCCACCACGGCCTGCTGGCCACCGGTGCGAGC 
ATCGAGGAGGCCTGCGTGATCGCGGTGCTGCTCGAGCGCGCGGCGAAGATGCAGTTGCTGGCGATGGCCGCT 
GGGGAGATCAAGCCGATCCCGCCGGCCCTGGCGCGCGAGGCCCACGACTGGATCTCCACCCCGAAGCGCCAC 
GGCGCCGCATTCAACTACTACGCCCGGCGCTGCCTGCCGCGCAACAGCGACTGCCTGAGCTGA