ViewGene
Locus Name:
GeneID:
Location:

GeneID:2935
TrivialName: PA14_10670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10670
PAO1 Ortholog Locus:PA4119
Sequence Length:804
Protein Length:267
Start: 919472
Stop: 920275
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07506.1|AE004828_7 (AE004828) aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa PAO1]
Identities = 266/268 (99%), Positives = 266/268 (99%), Gaps = 1/268 (0%)
Sequence:
ATGCATGATGCAGCCACCTCCATGCCGCAGGCTCCCTCAACCTGGGCCGACTACCTTGCCGGCTACCGCTGG
CGAGGGCAGGGCGAAGGATGTTCCGCGGCCACGGTCCACCGCCTGGAGGCTGAGCGGCGGCCGACCCTGTTC
GTCAAGCAGGAAGTGCTGTCCGCACATGCCGAGCTGCCCGCCGAAATCGCCCGCCTGCGCTGGCTGCACGGT
GCCGGCATCGATTGCCCGCAGGTGCTGAACGAAACCCAGAGCGACGGCCGGCAATGGCTGCTGATGAGCGCA
GTGCCGGGGGACACGCTGTCCGCGCTGGCGCAGCGCGGCGAGCTGGAGCCCGAGCGCCTGGTGCGCCTGGTG
GCCGCCGCCCTGCGCCGGCTGCACGATCTCGATCCGGCCGCCTGTCCCTTCGACCATCGCCTGGAACGGCGT
CTGGACACCGTGCGCCAGCGGGTCGAGGCCGGGCTGGTGGACGAGGCGGACTTCGACGACGACCATCGCGGT
CGCAGCGCCACGGAGCTGTATCGCCTGCTGCTCGACCGGCGTCCGGCGGTCGAAGACCTGGTGGTCGCCCAT
GGCGACGCCTGCCTGCCAAATCTGCTGGCGGAGGGGCGGCGCTTCAGCGGCTTCATCGATTGCGGGCGGCTC
GGCGTCGCCGACCGGCACCAGGACCTGGCCCTGGCCGCGCGGGACATCGAGGCCGAACTCGGCGCGGCCTGG
GCCGAGGCCTTCCTCGTCGAATACGGCGGCGATATCGACGGCGAACGGCTGGCGTACTTCAGGCTATTGGAC
GAGTTCTTCTAG
Translation:
MHDAATSMPQAPSTWADYLAGYRWRGQGEGCSAATVHRLEAERRPTLFVKQEVLSAHAELPAEIARLRWLHG
AGIDCPQVLNETQSDGRQWLLMSAVPGDTLSALAQRGELEPERLVRLVAAALRRLHDLDPAACPFDHRLERR
LDTVRQRVEAGLVDEADFDDDHRGRSATELYRLLLDRRPAVEDLVVAHGDACLPNLLAEGRRFSGFIDCGRL
GVADRHQDLALAARDIEAELGAAWAEAFLVEYGGDIDGERLAYFRLLDEFF*
AnnotationID:2943GeneID:2935
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 12:12:52
Gene Name:aph
Confidence Code:1
GeneProduct:aminoglycoside 3'-phosphotransferase type IIb
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:neomycin phosphotransferase
Functional Category Confidence Code:5
COGs:COG3231,pfam01636
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG09774.1|AF244993_1 (AF244993) aminoglycoside 3'-phosphotransferase [Vibrio cholerae]
          Length = 264

 Score =  270 bits (690), Expect = 6e-72
 Identities = 137/258 (53%), Positives = 170/258 (65%), Gaps = 5/258 (1%)

>gb|AAA73390.1| (U00004) aminoglycoside-3'-O-phosphotransferase [Escherichia coli]
          Length = 264

 Score =  270 bits (690), Expect = 6e-72
 Identities = 137/258 (53%), Positives = 170/258 (65%), Gaps = 5/258 (1%)








 
Structural Features:
COG3231, Aph, Aminoglycoside phosphotransferase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 266 residues,  99.6% aligned
Score =  314 bits (805), Expect = 9e-87
Identities = 148/267 (55%), Positives = 173/267 (64%), Gaps = 4/267 (1%)

pfam01636, APH, Phosphotransferase enzyme family.
CD-Length = 241 residues,  99.2% aligned
Score =  133 bits (336), Expect = 2e-32
Identities = 93/243 (38%), Positives = 114/243 (46%), Gaps = 10/243 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5873




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23015




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36878




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2935_cdd.html


    		




 
URL:

















    ReferenceID:36953




  
Author/Investigator(s):
        Hachler H, Santanam P, Kayser FH.





  
Title:
        Sequence and characterization of a novel chromosomal aminoglycoside phosphotransferase gene, aph (3')-IIb, in Pseudomonas aeruginosa.




  
PubMed:
        8723476


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1996 May;40(5):1254-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8723476









           
Homologs By Global Alignment 

           

       



     
Gene ID:2935
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4750
                           PA4119_tr
                           translation of PA4119
                           268
                           99.25
                           99.25
                           1
                           1379.0
     

     
     
136320
                           gb|AAG07506.1|AE004828_7
                           (AE004828) aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa PAO1]
                           268
                           99.25
                           99.25
                           1
                           1379.0
     

     
     
136321
                           emb|CAA62365.1|
                           (X90856) aminoglycoside phosphotransferase (3')IIps [Pseudomonas aeruginosa]
                           268
                           98.50
                           98.88
                           1
                           1369.0
     

     
     
136322
                           emb|CAA23892.1|
                           (V00618) neomycin phosphotransferase [Escherichia coli]
                           275
                           50.90
                           63.27
                           19
                           692.0
     

     
     
136323
                           gb|AAG09774.1|AF244993_1
                           (AF244993) aminoglycoside 3'-phosphotransferase [Vibrio cholerae]
                           275
                           50.90
                           63.27
                           19
                           692.0
     

     






      

          
GC ORFID: 45937How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4119Glimmer Score: 

          
Start: 4034465Stop: 4033662

          
Length: 804


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4119 translation of PA4119Homolog Bit Score 535.0

          
Other Homologs:  t_PA4119 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCATGATGCAGCCACCTCCATGCCGCAGGCTCCCTCAACCTGGGCCGACTACCTTGCCGGCTACCGCTGG 
CGAGGGCAGGGCGAAGGATGTTCCGCGGCCACGGTCCACCGCCTGGAGGCTGAGCGGCGGCCGACCCTGTTC 
GTCAAGCAGGAAGTGCTGTCCGCACATGCCGAGCTGCCCGCCGAAATCGCCCGCCTGCGCTGGCTGCACGGT 
GCCGGCATCGATTGCCCGCAGGTGCTGAACGAAACCCAGAGCGACGGCCGGCAATGGCTGCTGATGAGCGCA 
GTGCCGGGGGACACGCTGTCCGCGCTGGCGCAGCGCGGCGAGCTGGAGCCCGAGCGCCTGGTGCGCCTGGTG 
GCCGCCGCCCTGCGCCGGCTGCACGATCTCGATCCGGCCGCCTGTCCCTTCGACCATCGCCTGGAACGGCGT 
CTGGACACCGTGCGCCAGCGGGTCGAGGCCGGGCTGGTGGACGAGGCGGACTTCGACGACGACCATCGCGGT 
CGCAGCGCCACGGAGCTGTATCGCCTGCTGCTCGACCGGCGTCCGGCGGTCGAAGACCTGGTGGTCGCCCAT 
GGCGACGCCTGCCTGCCAAATCTGCTGGCGGAGGGGCGGCGCTTCAGCGGCTTCATCGATTGCGGGCGGCTC 
GGCGTCGCCGACCGGCACCAGGACCTGGCCCTGGCCGCGCGGGACATCGAGGCCGAACTCGGCGCGGCCTGG 
GCCGAGGCCTTCCTCGTCGAATACGGCGGCGATATCGACGGCGAACGGCTGGCGTACTTCAGGCTATTGGAC 
GAGTTCTTCTAG