ViewGene
Locus Name:
GeneID:
Location:

GeneID:2933
TrivialName: PA14_20400
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20400
PAO1 Ortholog Locus:PA3376
Sequence Length:816
Protein Length:271
Start: 1754862
Stop: 1755677
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06764.1|AE004759_11 (AE004759) probable ATP-binding component of ABC transporter [Pseudomonas aeruginosa PAO1]
Identities = 271/271 (100%), Positives = 271/271 (100%)
Sequence:
ATGAACGCGGTCTGCGAATCCATCGATGCGAGCCTCGACGCGCGCGACCGGCCGCTGCTCTCGGTGCGCGGC
CTGACCCGCCTGTACGGGCCGGAAAAGGGCTGCCAGGAGGTCGACTTCGATCTCTATCCCGGCGAAGTGCTG
GGCATCGTCGGCGAGTCCGGCTCGGGCAAGTCGACCCTGCTCTCGCTGCTCTCCGGACGCTGTCCGCCGGAC
GCCGGCACGGTCGCCTACCGCGACGACGGCGACCGCTGGCTGGACCTCTACGCCGCCAGCGAAGCCGAGCGG
CGCACCCTGCTGCGCACCGAGTGGGGCTTCGTCGAGCAGAACCCGCGCGACGGCCTGCGCATGGGCGTGTCC
GCCGGCGCCAACATCGGCGAACGGCTGATGGCCCAGGGCGTGCGCCATTACGGTCGGCTGCGCCAGGCCGGG
CTGGACTGGCTGGAACAGGTGGAGATCGATCCGCTGCGCATCGACGACCTGCCGCGAACCTTTTCCGGCGGC
ATGCAGCAGCGCCTGCAGATCGCCCGCAACCTGGTCTCGGCGCCACGCCTGGTGTTCATGGACGAACCCACC
GGCGGCCTCGACGTGTCGGTGCAGGCGCGCCTGCTCGACCTGCTGCGCGGCCTGGTGCGCGAGCTGGACCTG
GCGGTGGTGATCGTCACCCACGACCTGGCGGTGGCGCGCCTGCTCGCCGACCGCCTGATGGTCATGCGCCGT
TCGCGGGTGGTGGAAGCCGGTCTCACCGACCAGATTCTCGACGACCCGCAGCATCCGTACACGCAATTGCTG
GTGTCGTCGGTCCTGCAACCCTGA
Translation:
MNAVCESIDASLDARDRPLLSVRGLTRLYGPEKGCQEVDFDLYPGEVLGIVGESGSGKSTLLSLLSGRCPPD
AGTVAYRDDGDRWLDLYAASEAERRTLLRTEWGFVEQNPRDGLRMGVSAGANIGERLMAQGVRHYGRLRQAG
LDWLEQVEIDPLRIDDLPRTFSGGMQQRLQIARNLVSAPRLVFMDEPTGGLDVSVQARLLDLLRGLVRELDL
AVVIVTHDLAVARLLADRLMVMRRSRVVEAGLTDQILDDPQHPYTQLLVSSVLQP*
AnnotationID:2941GeneID:2933
AnnotatorUID: danlee
Modification Date/Time: 2005-07-29 14:02:51
Gene Name:phnK
Confidence Code:3
GeneProduct:putative phosphonate ABC transporter, ATP-binding protein
Cell Localization:()
Synonyms:PA3376
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:HisP-like nucleotide binding protein (phnK)
Functional Category Confidence Code:5
COGs:cd00267,COG4107
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56068.1| (AE016865) phosphonate ABC transporter, ATP-binding protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 273

 Score =  451 bits (1159), Expect = e-126
 Identities = 227/255 (89%), Positives = 238/255 (93%)

>gb|AAN83527.1|AE016771_38 (AE016771) Phosphonates transport ATP-binding protein phnK
           [Escherichia coli CFT073]
          Length = 262

 Score =  352 bits (904), Expect = 1e-96
 Identities = 183/255 (71%), Positives = 205/255 (80%), Gaps = 5/255 (1%)

>gb|AAM84313.1|AE013674_9 (AE013674) ATP-binding component of phosphonate ABC transporter
           [Yersinia pestis KIM]
          Length = 285

 Score =  348 bits (893), Expect = 2e-95
 Identities = 179/260 (68%), Positives = 204/260 (78%)








 
Structural Features:
cd00267, ABC_ATPase, ABC (ATP-binding cassette) transporter 
nucleotide-binding domain
CD-Length = 217 residues,  99.1% aligned
Score =  157 bits (399), Expect = 1e-39
Identities = 83/229 (36%), Positives = 122/229 (53%), Gaps = 15/229 (6%)

COG4107, PhnK, ABC-type phosphonate transport system, ATPase component 
[Inorganic ion transport and metabolism]
CD-Length = 258 residues, 100.0% aligned
Score =  435 bits (1119), Expect = 3e-123
Identities = 205/258 (79%), Positives = 222/258 (86%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5869




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23011




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32186




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2933_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2933
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4747
                           PA3376_tr
                           translation of PA3376
                           271
                           99.99
                           99.99
                           0
                           1379.0
     

     
     
136272
                           gb|AAG06764.1|AE004759_11
                           (AE004759) probable ATP-binding component of ABC transporter [Pseudomonas aeruginosa PAO1]
                           271
                           99.99
                           99.99
                           0
                           1379.0
     

     
     
136273
                           gb|AAO56068.1|
                           (AE016865) phosphonate ABC transporter, ATP-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           279
                           83.51
                           87.81
                           14
                           1151.0
     

     
     
136274
                           dbj|BAB54545.1|
                           (AP003015) ABC transporter ATP-binding protein [Mesorhizobium loti]
                           271
                           69.00
                           77.49
                           6
                           918.0
     

     
     
136278
                           gb|AAA24349.1|
                           (J05260) HisP-like nucleotide binding protein (phnK) [Escherichia coli]
                           271
                           67.52
                           75.64
                           19
                           901.0
     

     






      

          
GC ORFID: 45935How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3376Glimmer Score: 

          
Start: 3199070Stop: 3198255

          
Length: 816


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3376 translation of PA3376Homolog Bit Score 535.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1224 (ORF: BLASTX 447.0), t_PA3376 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGCGGTCTGCGAATCCATCGATGCGAGCCTCGACGCGCGCGACCGGCCGCTGCTCTCGGTGCGCGGC 
CTGACCCGCCTGTACGGGCCGGAAAAGGGCTGCCAGGAGGTCGACTTCGATCTCTATCCCGGCGAAGTGCTG 
GGCATCGTCGGCGAGTCCGGCTCGGGCAAGTCGACCCTGCTCTCGCTGCTCTCCGGACGCTGTCCGCCGGAC 
GCCGGCACGGTCGCCTACCGCGACGACGGCGACCGCTGGCTGGACCTCTACGCCGCCAGCGAAGCCGAGCGG 
CGCACCCTGCTGCGCACCGAGTGGGGCTTCGTCGAGCAGAACCCGCGCGACGGCCTGCGCATGGGCGTGTCC 
GCCGGCGCCAACATCGGCGAACGGCTGATGGCCCAGGGCGTGCGCCATTACGGTCGGCTGCGCCAGGCCGGG 
CTGGACTGGCTGGAACAGGTGGAGATCGATCCGCTGCGCATCGACGACCTGCCGCGAACCTTTTCCGGCGGC 
ATGCAGCAGCGCCTGCAGATCGCCCGCAACCTGGTCTCGGCGCCACGCCTGGTGTTCATGGACGAACCCACC 
GGCGGCCTCGACGTGTCGGTGCAGGCGCGCCTGCTCGACCTGCTGCGCGGCCTGGTGCGCGAGCTGGACCTG 
GCGGTGGTGATCGTCACCCACGACCTGGCGGTGGCGCGCCTGCTCGCCGACCGCCTGATGGTCATGCGCCGT 
TCGCGGGTGGTGGAAGCCGGTCTCACCGACCAGATTCTCGACGACCCGCAGCATCCGTACACGCAATTGCTG 
GTGTCGTCGGTCCTGCAACCCTGA