ViewGene
Locus Name:
GeneID:
Location:

GeneID:2930
TrivialName: PA14_43830
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43830
PAO1 Ortholog Locus:PA1598
Sequence Length:822
Protein Length:273
Start: 3902443
Stop: 3903264
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04987.1|AE004587_11 (AE004587) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 273/273 (100%), Positives = 273/273 (100%)
Sequence:
ATGAGCAGTCATCGCCCGCAACATCGCCTGTCCGTTCCCGATATCCAGCGCCGCAAGGGTGCCGGCAGCCTG
GTGGCGCTGACCGCCTACAGCACGCCCATGGCCCGCCTCCTCGATCCGCACGCCGACCTGCTGCTGGTCGGC
GATTCGCTGGGCATGGTGCTCTATGGCATGCCCAGTACCCTGGGAGTGAGCCTGGAGATGATGGTCGCCCAC
ACCCTGGCGGTGATGCGCGGCAGCCGGCGCGCCTGCGTGGTGGCCGACTTGCCGTTCGCCAGCTACCAGGAA
TCGCCCCGACAGGCGTTCCGCAACGCCGCACGCCTGCTGGCCGACAGCGGCGCCCAGGCGGTGAAGCTGGAA
GGCGGTGAGGAAATGGAAGAAACCGTGGACTTCCTGGTACGCCGTGGCATACCGGTGCTCGCCCATATCGGC
CTGATGCCGCAGCAGGTCAACGCCATGGGCGGCTTCAAGGCCCAGGGGCGTGACCCGGAAAGCGCCGAACGG
GTACGCCGCGACGGCCTCGCCATGCAGCGCGGCGGCGCCTTCGCGGTGGTCATCGAAGGCGTAGGCGAGCCG
CTGGCCCGTCGCCTTAGCGAAGAACTGGCGATCCCCTGCATCGGTATCGGCGCCTCGCCGGCCTGCGACGGC
CAGGTACTGGTCAGCGAGGACCTGCTCGGGCTAAGCGGCGAACAGGTACCGCGCTTCGTCGAACGCTACGCG
CGCCTCGACCGGGAAATCGACGAGGCGGCCCGGCGCTTCGCCGAGGACGTGCGCGAACGGCGCTTCCCCGag
gCCAGGCATTGCTTCGCCATGCGCGAGTGA
Translation:
MSSHRPQHRLSVPDIQRRKGAGSLVALTAYSTPMARLLDPHADLLLVGDSLGMVLYGMPSTLGVSLEMMVAH
TLAVMRGSRRACVVADLPFASYQESPRQAFRNAARLLADSGAQAVKLEGGEEMEETVDFLVRRGIPVLAHIG
LMPQQVNAMGGFKAQGRDPESAERVRRDGLAMQRGGAFAVVIEGVGEPLARRLSEELAIPCIGIGASPACDG
QVLVSEDLLGLSGEQVPRFVERYARLDREIDEAARRFAEDVRERRFPEARHCFAMRE*
AnnotationID:2938GeneID:2930
AnnotatorUID: diggins
Modification Date/Time: 2005-05-18 08:08:50
Gene Name:
Confidence Code:3
GeneProduct:putative 3-methyl-2-oxobutanoate hydroxymethyltransferase
Cell Localization:()
Synonyms:panB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0413,pfam02548
Secondary Functional Category(ies):coenzyme metabolism
EC Number:2.1.2.11
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL52773.1| (AE009595) 3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE
           [Brucella melitensis 16M]
          Length = 288

 Score =  327 bits (838), Expect = 5e-89
 Identities = 164/272 (60%), Positives = 205/272 (75%)

>gb|AAL44296.1| (AE009278) 3-methyl-2-oxobutanoate hydroxymethyltransferase
           [Agrobacterium tumefaciens str. C58 (U. Washington)]
          Length = 279

 Score =  327 bits (838), Expect = 5e-89
 Identities = 159/273 (58%), Positives = 203/273 (74%)






 
Structural Features:
COG0413, PanB, Ketopantoate hydroxymethyltransferase 
[Coenzyme metabolism]
CD-Length = 268 residues,  99.6% aligned
Score =  362 bits (931), Expect = 2e-101
Identities = 144/267 (53%), Positives = 185/267 (69%), Gaps = 1/267 (0%)

pfam02548, Pantoate_transf, Ketopantoate hydroxymethyltransferase. 
CD-Length = 261 residues,  96.9% aligned
Score =  306 bits (785), Expect = 2e-84
Identities = 131/253 (51%), Positives = 173/253 (68%), Gaps = 2/253 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5863




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23005




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37275




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2930_cdd.html


    		




 
URL:

















    ReferenceID:38145




  
Author/Investigator(s):
        Merkel WK, Nichols BP.





  
Title:
        Characterization and sequence of the Escherichia coli panBCD gene cluster.




  
PubMed:
        8837478


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1996 Oct 1;143(2-3):247-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8837478

















    ReferenceID:38146




  
Author/Investigator(s):
        Jones CE, Brook JM, Buck D, Abell C, Smith AG.





  
Title:
        Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme.




  
PubMed:
        8096212


MedLine:
        




    
Source:
J Bacteriol. 1993 Apr;175(7):2125-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8096212









           
Homologs By Global Alignment 

           

       



     
Gene ID:2930
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4742
                           PA1598_tr
                           translation of PA1598
                           273
                           99.99
                           99.99
                           0
                           1377.0
     

     
     
136157
                           gb|AAG04987.1|AE004587_11
                           (AE004587) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           273
                           99.99
                           99.99
                           0
                           1377.0
     

     
     
136159
                           gb|AAN29279.1|AE014344_14
                           (AE014344) 3-methyl-2-oxobutanoate hydroxymethyltransferase [Brucella suis 1330]
                           275
                           59.63
                           74.54
                           2
                           837.0
     

     
     
136158
                           gb|AAL52773.1|
                           (AE009595) 3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE [Brucella melitensis 16M]
                           289
                           57.78
                           70.93
                           17
                           840.0
     

     
     
136162
                           emb|CAC46740.1|
                           (AL591789) PROBABLE 3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE PROTEIN [Sinorhizobium meliloti]
                           273
                           57.14
                           73.26
                           0
                           810.0
     

     






      

          
GC ORFID: 45932How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1598Glimmer Score: 

          
Start: 1051466Stop: 1050645

          
Length: 822


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1598 translation of PA1598Homolog Bit Score 535.0

          
Other Homologs:  t_PA4729 (ORF: BLASTX 181.0), t_PA1598 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAGTCATCGCCCGCAACATCGCCTGTCCGTTCCCGATATCCAGCGCCGCAAGGGTGCCGGCAGCCTG 
GTGGCGCTGACCGCCTACAGCACGCCCATGGCCCGCCTCCTCGATCCGCACGCCGACCTGCTGCTGGTCGGC 
GATTCGCTGGGCATGGTGCTCTATGGCATGCCCAGTACCCTGGGAGTGAGCCTGGAGATGATGGTCGCCCAC 
ACCCTGGCGGTGATGCGCGGCAGCCGGCGCGCCTGCGTGGTGGCCGACTTGCCGTTCGCCAGCTACCAGGAA 
TCGCCCCGACAGGCGTTCCGCAACGCCGCACGCCTGCTGGCCGACAGCGGCGCCCAGGCGGTGAAGCTGGAA 
GGCGGTGAGGAAATGGAAGAAACCGTGGACTTCCTGGTACGCCGTGGCATACCGGTGCTCGCCCATATCGGC 
CTGATGCCGCAGCAGGTCAACGCCATGGGCGGCTTCAAGGCCCAGGGGCGTGACCCGGAAAGCGCCGAACGG 
GTACGCCGCGACGGCCTCGCCATGCAGCGCGGCGGCGCCTTCGCGGTGGTCATCGAAGGCGTAGGCGAGCCG 
CTGGCCCGTCGCCTTAGCGAAGAACTGGCGATCCCCTGCATCGGTATCGGCGCCTCGCCGGCCTGCGACGGC 
CAGGTACTGGTCAGCGAGGACCTGCTCGGGCTAAGCGGCGAACAGGTACCGCGCTTCGTCGAACGCTACGCG 
CGCCTCGACCGGGAAATCGACGAGGCGGCCCGGCGCTTCGCCGAGGACGTGCGCGAACGGCGCTTCCCCGag 
gCCAGGCATTGCTTCGCCATGCGCGAGTGA