ViewGene
Locus Name:
GeneID:
Location:

GeneID:2926
TrivialName: PA14_04670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04670
PAO1 Ortholog Locus:PA0357
Sequence Length:813
Protein Length:270
Start: 415292
Stop: 416104
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03746.1|AE004473_8 (AE004473) formamidopyrimidine-DNA glycosylase [Pseudomonas aeruginosa PAO1]
Identities = 270/270 (100%), Positives = 270/270 (100%)
Sequence:
ATGCCCGAACTACCCGAAGTCGAAACCACCCGCCGGGGCATCGCCCCCTACCTCGAAGGCCAGCGCGTCGAG
CGCGTGATCGTGCGCGAGCGGCGCCTGCGCTGGCCGATCCCCGAAGACCTGGACGTGCGCCTGTCCGGACAA
CGCATCGTCAGCGTCGAGCGCCGCGCCAAGTACCTGCTGCTGGGCGCCGAGGCCGGTACGCTGATCAGCCAC
CTGGGCATGTCCGGCAGCCTGAGGCTGGTGGAGAGCGGCACCCCGGCCAGTCGCCACGAGCACGTCGACATC
GAGCTGGCCTCGGGCATGGCCCTGCGCTACACCGATCCGCGGCGCTTCGGCGCCATGCTCTGGAGCCTGGCG
CCACTGGAACACGAGCTGCTGCGCAACCTCGGCCCGGAACCGCTGACCGACGCTTTCGCCGGCCAGCGTCTG
TTCGAGCTGTCGCGCGGGCGGAGCATGGCGGTCAAGCCGTTCATCATGGACAACGCGGTGGTGGTCGGGGTC
GGCAACATCTACGCCAGCGAAGCGCTGTTCGCCGCCGGCATCGATCCGCGCAAGCCCGCCGGGAGCATCTCC
AAGGCGCGCTACCTGCGCCTGGCGGAGGAGATCAAGCGGATCCTGGCGATCGCCATCGAGCGCGGCGGCACC
ACCCTGCGCGATTTCGTCGGCGGCGACGGCCAGCCCGGTTACTTCCAGCAGGAATTGTTCGTCTATGGCCGT
GGCGGGGAGTTCTGCAAGGTCTGCGGCAGCACCCTGCGCGAGATCCGCCTAGGCCAGCGCGCCAGCGTGTAC
TGCCCGCGCTGCCAACGCTGA
Translation:
MPELPEVETTRRGIAPYLEGQRVERVIVRERRLRWPIPEDLDVRLSGQRIVSVERRAKYLLLGAEAGTLISH
LGMSGSLRLVESGTPASRHEHVDIELASGMALRYTDPRRFGAMLWSLAPLEHELLRNLGPEPLTDAFAGQRL
FELSRGRSMAVKPFIMDNAVVVGVGNIYASEALFAAGIDPRKPAGSISKARYLRLAEEIKRILAIAIERGGT
TLRDFVGGDGQPGYFQQELFVYGRGGEFCKVCGSTLREIRLGQRASVYCPRCQR*
AnnotationID:2934GeneID:2926
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 18:06:53
Gene Name:mutM
Confidence Code:1
GeneProduct:formamidopyrimidine-DNA glycosylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:mismatch repair protein MutM
Functional Category Confidence Code:5
COGs:COG0266,pfam01149,pfam06831
Secondary Functional Category(ies):
EC Number:3.2.2.23
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70690.1|AE016712_8 (AE016793) formamidopyrimidine-DNA glycosylase [Pseudomonas putida
           KT2440]
          Length = 270

 Score =  471 bits (1212), Expect = e-132
 Identities = 230/270 (85%), Positives = 251/270 (92%)

>gb|AAO53958.1| (AE016857) formamidopyrimidine-DNA glycosylase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 270

 Score =  449 bits (1155), Expect = e-126
 Identities = 221/270 (81%), Positives = 244/270 (90%)








 
Structural Features:
COG0266, Nei, Formamidopyrimidine-DNA glycosylase 
[DNA replication, recombination, and repair]
CD-Length = 273 residues, 100.0% aligned
Score =  355 bits (912), Expect = 3e-99
Identities = 155/273 (56%), Positives = 185/273 (67%), Gaps = 3/273 (1%)

pfam01149, Fapy_DNA_glyco, Formamidopyrimidine-DNA glycosylase N-terminal domain.
CD-Length = 119 residues,  99.2% aligned
Score =  128 bits (322), Expect = 8e-31
Identities = 60/118 (50%), Positives = 72/118 (61%), Gaps = 2/118 (1%)

pfam06831, H2TH, Formamidopyrimidine-DNA glycosylase H2TH domain.
CD-Length = 93 residues, 100.0% aligned
Score =  107 bits (269), Expect = 1e-24
Identities = 45/93 (48%), Positives = 56/93 (60%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5855




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22997




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33697




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2926_cdd.html


    		




 
URL:

















    ReferenceID:35406




  
Author/Investigator(s):
        Oliver A, Sanchez JM, Blazquez J.





  
Title:
        Characterization of the GO system of Pseudomonas aeruginosa.




  
PubMed:
        12445642


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2002 Nov 19;217(1):31-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12445642









           
Homologs By Global Alignment 

           

       



     
Gene ID:2926
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4738
                           PA0357_tr
                           translation of PA0357
                           270
                           99.99
                           99.99
                           0
                           1382.0
     

     
     
136007
                           gb|AAG03746.1|AE004473_8
                           (AE004473) formamidopyrimidine-DNA glycosylase [Pseudomonas aeruginosa PAO1]
                           270
                           99.99
                           99.99
                           0
                           1382.0
     

     
     
136008
                           gb|AAF42853.1|
                           (AF220059) mismatch repair protein MutM [Pseudomonas aeruginosa]
                           270
                           99.99
                           99.99
                           0
                           1382.0
     

     
     
136009
                           gb|AAN70690.1|AE016712_8
                           (AE016793) formamidopyrimidine-DNA glycosylase [Pseudomonas putida KT2440]
                           270
                           85.18
                           92.96
                           0
                           1212.0
     

     
     
136010
                           gb|AAO53958.1|
                           (AE016857) formamidopyrimidine-DNA glycosylase [Pseudomonas syringae pv. tomato str. DC3000]
                           270
                           81.85
                           90.37
                           0
                           1155.0
     

     






      

          
GC ORFID: 45928How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0357Glimmer Score: 

          
Start: 6216634Stop: 6217446

          
Length: 813


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0357 translation of PA0357Homolog Bit Score 536.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6601 (ORF: BLASTX 460.0), t_PA0357 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCGAACTACCCGAAGTCGAAACCACCCGCCGGGGCATCGCCCCCTACCTCGAAGGCCAGCGCGTCGAG 
CGCGTGATCGTGCGCGAGCGGCGCCTGCGCTGGCCGATCCCCGAAGACCTGGACGTGCGCCTGTCCGGACAA 
CGCATCGTCAGCGTCGAGCGCCGCGCCAAGTACCTGCTGCTGGGCGCCGAGGCCGGTACGCTGATCAGCCAC 
CTGGGCATGTCCGGCAGCCTGAGGCTGGTGGAGAGCGGCACCCCGGCCAGTCGCCACGAGCACGTCGACATC 
GAGCTGGCCTCGGGCATGGCCCTGCGCTACACCGATCCGCGGCGCTTCGGCGCCATGCTCTGGAGCCTGGCG 
CCACTGGAACACGAGCTGCTGCGCAACCTCGGCCCGGAACCGCTGACCGACGCTTTCGCCGGCCAGCGTCTG 
TTCGAGCTGTCGCGCGGGCGGAGCATGGCGGTCAAGCCGTTCATCATGGACAACGCGGTGGTGGTCGGGGTC 
GGCAACATCTACGCCAGCGAAGCGCTGTTCGCCGCCGGCATCGATCCGCGCAAGCCCGCCGGGAGCATCTCC 
AAGGCGCGCTACCTGCGCCTGGCGGAGGAGATCAAGCGGATCCTGGCGATCGCCATCGAGCGCGGCGGCACC 
ACCCTGCGCGATTTCGTCGGCGGCGACGGCCAGCCCGGTTACTTCCAGCAGGAATTGTTCGTCTATGGCCGT 
GGCGGGGAGTTCTGCAAGGTCTGCGGCAGCACCCTGCGCGAGATCCGCCTAGGCCAGCGCGCCAGCGTGTAC 
TGCCCGCGCTGCCAACGCTGA