ViewGene
Locus Name:
GeneID:
Location:

GeneID:2916
TrivialName: PA14_25710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25710
PAO1 Ortholog Locus:PA2964
Sequence Length:816
Protein Length:271
Start: 2245770
Stop: 2246585
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06352.1|AE004722_8 (AE004722) 4-amino-4-deoxychorismate lyase [Pseudomonas aeruginosa PAO1]
Identities = 269/271 (99%), Positives = 269/271 (99%)
Sequence:
ATGCTGGACTGGGTCGACGGCCGGCCCGCCGCCGAGCTGTCCGTGCGCGATCGCGGACTGGCCTATGGCGAC
GGGCTGTTCGAGACCCTGGCGGTGCGCGCCGGCACGCCGCGTCTCCTGGAGCGCCACCTGGCGCGCCTGGAA
GAGGGGTGTCGACGCCTCGCGATCCCTCTCGACGCCGCAGCGTTGCGCCAGGAGTTGCTGGCGTTCTGCGCG
GCGCTGGGCGACGGTGTCGCCAAGCTGATCGTCACCCGCGGCGAGGGTTTGCGTGGCTACGCGCCGCCTGCC
GAGGCCTCGCCGCGGCGAATCCTCTCCGGATCGCCGCGTCCGGCCTATCCCGAGCGCCACTGGCAGCAAGGC
GTGCGCCTGTTCGCCTGCCGTACGCGCCTGGCCGAGCAGCCCCTGCTGGCCGGCCTCAAGCACCTCAATCGC
CTGGAGCAGGTCCTCGCCCGTGCCGAGTGGGGCGACGCCGGGCATGCCGAAGGGCTCATGCTGGACGTGCAC
GAGCGGGTGGTGGAGGGGGTGTTCAGCAACCTGCTGCTGGTTCTCGACGGCACCCTCGTGGCGCCTGACCTG
CGGCGCTGCGGCGTCGCGGGAGTGATGCGCGCGGAATTGCTCGAGCGCGCCGAAGGAATCGGCGTCCCGCTG
GCGATACGGGATGTTTCCATGGCTGAACTGGCGACGGCCGACGAGGTCTTTCTGTGCAACAGCCAGTTCGGC
ATCTGGCCGGTCCGGGCATTGGACGAGCACGTCTGGCCGGTCGGCGAGCTGACCCGTAAACTGCAAGACCAA
CTTCGCGACGACCTGGATTTCTGA
Translation:
MLDWVDGRPAAELSVRDRGLAYGDGLFETLAVRAGTPRLLERHLARLEEGCRRLAIPLDAAALRQELLAFCA
ALGDGVAKLIVTRGEGLRGYAPPAEASPRRILSGSPRPAYPERHWQQGVRLFACRTRLAEQPLLAGLKHLNR
LEQVLARAEWGDAGHAEGLMLDVHERVVEGVFSNLLLVLDGTLVAPDLRRCGVAGVMRAELLERAEGIGVPL
AIRDVSMAELATADEVFLCNSQFGIWPVRALDEHVWPVGELTRKLQDQLRDDLDF*
AnnotationID:2924GeneID:2916
AnnotatorUID: danlee
Modification Date/Time: 2006-01-31 15:03:35
Gene Name:pabC
Confidence Code:2
GeneProduct:4-amino-4-deoxychorismate lyase
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd01559,cd01558,cd00449,COG0115
Secondary Functional Category(ies):
EC Number:4.1.3.38
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57297.1| (AE016869) 4-amino-4-deoxychorismate lyase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 271

 Score =  345 bits (885), Expect = 2e-94
 Identities = 175/270 (64%), Positives = 204/270 (75%)

>gb|AAN67535.1|AE016382_3 (AE016781) 4-amino-4-deoxychorismate lyase [Pseudomonas putida
           KT2440]
          Length = 271

 Score =  330 bits (846), Expect = 5e-90
 Identities = 166/270 (61%), Positives = 199/270 (73%)








 
Structural Features:
cd01559, ADCL_like, ADCL_like: 4-Amino-4-deoxychorismate lyase
CD-Length = 249 residues, 100.0% aligned
Score =  234 bits (599), Expect = 6e-63

cd01558, D-AAT_like, D-Alanine aminotransferase (D-AAT_like)
CD-Length = 270 residues, 100.0% aligned
Score =  149 bits (377), Expect = 4e-37

cd00449, PLPDE_IV, PyridoxaL 5'-Phosphate Dependent Enzymes class IV (PLPDE_IV).

COG0115, IlvE, Branched-chain amino acid 
aminotransferase/4-amino-4-deoxychorismate lyase 
[Amino acid transport and metabolism / Coenzyme metabolism] 
CD-Length = 284 residues,  97.5% aligned
Score =  192 bits (490), Expect = 3e-50
Identities = 102/278 (36%), Positives = 136/278 (48%), Gaps = 12/278 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5835




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22977




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36085




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2916_cdd.html


    		




 
URL:

















    ReferenceID:36434




  
Author/Investigator(s):
        Kutchma AJ, Hoang TT, Schweizer HP.





  
Title:
        Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD).




  
PubMed:
        10464226


MedLine:
        




    
Source:
J Bacteriol. 1999 Sep;181(17):5498-504.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10464226

















    ReferenceID:36435




  
Author/Investigator(s):
        Green JM, Merkel WK, Nichols BP.





  
Title:
        Characterization and sequence of Escherichia coli pabC, the gene encoding aminodeoxychorismate lyase, a pyridoxal phosphate-containing enzyme.




  
PubMed:
        1644759


MedLine:
        




    
Source:
J Bacteriol. 1992 Aug;174(16):5317-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1644759

















    ReferenceID:36436




  
Author/Investigator(s):
        Shen Z, Byers DM.





  
Title:
        Isolation of Vibrio harveyi acyl carrier protein and the fabG, acpP, and fabF genes involved in fatty acid biosynthesis.




  
PubMed:
        8550484


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(2):571-3.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550484









           
Homologs By Global Alignment 

           

       



     
Gene ID:2916
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4718
                           PA2964_tr
                           translation of PA2964
                           271
                           99.26
                           99.26
                           0
                           1387.0
     

     
     
135547
                           gb|AAG06352.1|AE004722_8
                           (AE004722) 4-amino-4-deoxychorismate lyase [Pseudomonas aeruginosa PAO1]
                           271
                           99.26
                           99.26
                           0
                           1387.0
     

     
     
135548
                           gb|AAO57297.1|
                           (AE016869) 4-amino-4-deoxychorismate lyase [Pseudomonas syringae pv. tomato str. DC3000]
                           271
                           64.57
                           75.27
                           0
                           885.0
     

     
     
135549
                           gb|AAN67535.1|AE016382_3
                           (AE016781) 4-amino-4-deoxychorismate lyase [Pseudomonas putida KT2440]
                           271
                           61.25
                           73.43
                           0
                           844.0
     

     
     
135550
                           gb|AAQ61074.1|
                           (AE016922) 4-amino-4-deoxychorismate lyase [Chromobacterium violaceum ATCC 12472]
                           272
                           40.07
                           54.41
                           3
                           499.0
     

     






      

          
GC ORFID: 45918How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2964Glimmer Score: 

          
Start: 2708155Stop: 2707340

          
Length: 816


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2964 translation of PA2964Homolog Bit Score 538.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2611 (ORF: BLASTX 348.0), t_PA2964 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGGACTGGGTCGACGGCCGGCCCGCCGCCGAGCTGTCCGTGCGCGATCGCGGACTGGCCTATGGCGAC 
GGGCTGTTCGAGACCCTGGCGGTGCGCGCCGGCACGCCGCGTCTCCTGGAGCGCCACCTGGCGCGCCTGGAA 
GAGGGGTGTCGACGCCTCGCGATCCCTCTCGACGCCGCAGCGTTGCGCCAGGAGTTGCTGGCGTTCTGCGCG 
GCGCTGGGCGACGGTGTCGCCAAGCTGATCGTCACCCGCGGCGAGGGTTTGCGTGGCTACGCGCCGCCTGCC 
GAGGCCTCGCCGCGGCGAATCCTCTCCGGATCGCCGCGTCCGGCCTATCCCGAGCGCCACTGGCAGCAAGGC 
GTGCGCCTGTTCGCCTGCCGTACGCGCCTGGCCGAGCAGCCCCTGCTGGCCGGCCTCAAGCACCTCAATCGC 
CTGGAGCAGGTCCTCGCCCGTGCCGAGTGGGGCGACGCCGGGCATGCCGAAGGGCTCATGCTGGACGTGCAC 
GAGCGGGTGGTGGAGGGGGTGTTCAGCAACCTGCTGCTGGTTCTCGACGGCACCCTCGTGGCGCCTGACCTG 
CGGCGCTGCGGCGTCGCGGGAGTGATGCGCGCGGAATTGCTCGAGCGCGCCGAAGGAATCGGCGTCCCGCTG 
GCGATACGGGATGTTTCCATGGCTGAACTGGCGACGGCCGACGAGGTCTTTCTGTGCAACAGCCAGTTCGGC 
ATCTGGCCGGTCCGGGCATTGGACGAGCACGTCTGGCCGGTCGGCGAGCTGACCCGTAAACTGCAAGACCAA 
CTTCGCGACGACCTGGATTTCTGA