ViewGene
Locus Name:
GeneID:
Location:

GeneID:2905
TrivialName: PA14_11760
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11760
PAO1 Ortholog Locus:PA4025
Sequence Length:822
Protein Length:273
Start: 1019336
Stop: 1018515
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07412.1|AE004819_5 (AE004819) probable ethanolamine ammonia-lyase light chain [Pseudomonas aeruginosa PAO1]
Identities = 272/273 (99%), Positives = 273/273 (100%)
Sequence:
ATGAACGACAAGCACCTGCCCGACGCCAGCGCCGAGAACCCCTGGCTGCCGCTGCGCCAGCTGACCCCCGCG
CGGATCGCCCTGGGCCGCACCGGCACCAGCCTGCCGACCCGCCCGCAACTGGACTTCCAGTACGCCCACGCC
CAGGCGCGCGACGCCGTGCACCTGCCCTTCGACCATGCGGCCATCAGCGACGGCCTGCGCCAGCGCGGCCGC
GACAGCCTGCTGCTGCACAGCGCCGCCGCCGACCGCCACGTCTACCTGCAACGCCCGGACCTCGGCCGGCGC
CTCGACGAGGCCTCGGTGCAACGCCTGCGCGAACACGCCGCCGGCTACGACGGGCAGATCGACCTGGCCATC
GTGGTCGCCGACGGCCTCTCCGCGCTGGCCGTGCAGCGTCACACCCTGCCCTTCCTCGAACGCCTGGAGGAA
CAGGCGCTGGCCGAAGGCTGGTCGCTGTCGCCGGTGGTCCTGGTGGAACAGGGACGGGTGGCGGTGGCCGAC
GAGATCGGCGAATTGCTGCGGGCGAAGATGAGCGTGATCCTGATCGGCGAGCGTCCCGGCCTCAGCTCGCCG
GACAGCCTCGGCCTGTACTTCACCTGGGCCCCGCGGGTCGGTCTCACCGACGCCTATCGCAACTGCATCTCC
AACGTCCGTCtGGAAGGCCTCAGCTACGGCATGGCCGCGCACCGCCTGCTCTACCTGATGCGCGAGGCCTGC
CGGCGGCAGCTTTCCGGGGTCAACCTGAAGGACGAGGCGGAGGTCCAGGCGCTGGAAGGCGAGGCTCCGCGC
ACCGGCAACTTCCTCCTGGCGAGGGACTGA
Translation:
MNDKHLPDASAENPWLPLRQLTPARIALGRTGTSLPTRPQLDFQYAHAQARDAVHLPFDHAAISDGLRQRGR
DSLLLHSAAADRHVYLQRPDLGRRLDEASVQRLREHAAGYDGQIDLAIVVADGLSALAVQRHTLPFLERLEE
QALAEGWSLSPVVLVEQGRVAVADEIGELLRAKMSVILIGERPGLSSPDSLGLYFTWAPRVGLTDAYRNCIS
NVRLEGLSYGMAAHRLLYLMREACRRQLSGVNLKDEAEVQALEGEAPRTGNFLLARD*
AnnotationID:2913GeneID:2905
AnnotatorUID: diggins
Modification Date/Time: 2006-02-06 21:09:04
Gene Name:
Confidence Code:3
GeneProduct:putative ethanolamine ammonia-lyase
Cell Localization:(1) Cytoplasmic
Synonyms:eutC
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4302,pfam05985
Secondary Functional Category(ies):carbon compound metabolism
EC Number:4.3.1.7
Status:ACTIVE
Pathway:
Ethanolamine utilization.
Glycerophospholipid metabolism.
Homology:
gb|AAL21351.1| (AE008810) ethanolamine ammonia-lyase, light chain
[Salmonella typhimurium LT2]
Length = 298
Score =  117 bits (294), Expect = 5e-26
Identities = 76/233 (32%), Positives = 121/233 (51%), Gaps = 10/233 (4%)
Structural Features:
gnl|CDD|13549 COG4302, EutC, Ethanolamine ammonia-lyase, small subunit
[Amino acid transport and metabolism]
Length = 294
Score =  340 bits (874), Expect = 2e-94
Identities = 134/253 (52%), Positives = 164/253 (64%), Gaps = 11/253 (4%)
Genomic Context:






   
Comment:


















    ReferenceID:5813




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22955




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34401




  
Author/Investigator(s):
        Faust LR, Connor JA, Roof DM, Hoch JA, Babior BM.





  
Title:
        Cloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium.




  
PubMed:
        2197274


MedLine:
        




    
Source:
J Biol Chem. 1990 Jul 25;265(21):12462-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=90324235&dopt=Citation

















    ReferenceID:38327




  
Author/Investigator(s):
        De Mot R, Nagy I, Schoofs G, Vanderleyden J.





  
Title:
        Sequence of Rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an APC-like permease.




  
PubMed:
        8069783


MedLine:
        




    
Source:
Can J Microbiol. 1994 May;40(5):403-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8069783&query_hl=3&itool=pubmed_docsum









           
Homologs By Global Alignment 

           

       



     
Gene ID:2905
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4705
                           PA4025_tr
                           translation of PA4025
                           273
                           99.63
                           99.99
                           0
                           1390.0
     

     
     
135345
                           gb|AAG07412.1|AE004819_5
                           (AE004819) probable ethanolamine ammonia-lyase light chain [Pseudomonas aeruginosa PAO1]
                           273
                           99.63
                           99.99
                           0
                           1390.0
     

     
     
135346
                           gb|AAN66169.1|AE016244_10
                           (AE016776) ethanolamine ammonia-lyase, light subunit, putative [Pseudomonas putida KT2440]
                           275
                           75.27
                           85.09
                           5
                           1050.0
     

     
     
135347
                           gb|AAO54268.1|
                           (AE016858) ethanolamine ammonia-lyase, light subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           289
                           70.93
                           82.00
                           18
                           1013.0
     

     
     
135348
                           emb|CAD16835.1|
                           (AL646073) PUTATIVE ETHANOLAMINE AMMONIA-LYASE SMALL SUBUNIT PROTEIN [Ralstonia solanacearum]
                           275
                           67.27
                           75.63
                           7
                           880.0
     

     






      

          
GC ORFID: 45907How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4025Glimmer Score: 

          
Start: 3934601Stop: 3935422

          
Length: 822


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4025 translation of PA4025Homolog Bit Score 540.0

          
Other Homologs:  t_PA4025 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGACAAGCACCTGCCCGACGCCAGCGCCGAGAACCCCTGGCTGCCGCTGCGCCAGCTGACCCCCGCG 
CGGATCGCCCTGGGCCGCACCGGCACCAGCCTGCCGACCCGCCCGCAACTGGACTTCCAGTACGCCCACGCC 
CAGGCGCGCGACGCCGTGCACCTGCCCTTCGACCATGCGGCCATCAGCGACGGCCTGCGCCAGCGCGGCCGC 
GACAGCCTGCTGCTGCACAGCGCCGCCGCCGACCGCCACGTCTACCTGCAACGCCCGGACCTCGGCCGGCGC 
CTCGACGAGGCCTCGGTGCAACGCCTGCGCGAACACGCCGCCGGCTACGACGGGCAGATCGACCTGGCCATC 
GTGGTCGCCGACGGCCTCTCCGCGCTGGCCGTGCAGCGTCACACCCTGCCCTTCCTCGAACGCCTGGAGGAA 
CAGGCGCTGGCCGAAGGCTGGTCGCTGTCGCCGGTGGTCCTGGTGGAACAGGGACGGGTGGCGGTGGCCGAC 
GAGATCGGCGAATTGCTGCGGGCGAAGATGAGCGTGATCCTGATCGGCGAGCGTCCCGGCCTCAGCTCGCCG 
GACAGCCTCGGCCTGTACTTCACCTGGGCCCCGCGGGTCGGTCTCACCGACGCCTATCGCAACTGCATCTCC 
AACGTCCGTCtGGAAGGCCTCAGCTACGGCATGGCCGCGCACCGCCTGCTCTACCTGATGCGCGAGGCCTGC 
CGGCGGCAGCTTTCCGGGGTCAACCTGAAGGACGAGGCGGAGGTCCAGGCGCTGGAAGGCGAGGCTCCGCGC 
ACCGGCAACTTCCTCCTGGCGAGGGACTGA